Article
Biotechnology & Applied Microbiology
Wannes Van Holm, Justien Ghesquiere, Nico Boon, Tim Verspecht, Kristel Bernaerts, Naiera Zayed, Ioanna Chatzigiannidou, Wim Teughels
Summary: The use of viability quantitative PCR (v-qPCR) has revolutionized the assessment of microbial viability, but the selection of qPCR amplicon length is crucial for accurate live/dead distinction. Longer amplicon lengths increase the difference in quantification cycle (C-q) between live and dead cells, but decrease qPCR efficiency. The optimal amplicon length for v-qPCR is around 400 bp, balancing efficiency with the ability to distinguish between live and dead bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Clemence Loublier, Bernard Taminiau, Julia Heinen, Laureline Lecoq, Helene Amory, Georges Daube, Carla Cesarini
Summary: This in vitro study aimed to assess the effect of a commonly used pre-FMT processing method on the bacterial composition and viability of the fecal filtrate. The results showed that the major bacterial populations in equine feces remained viable when using the described pre-FMT protocol, but the relative abundances of some minor genera were significantly different in the final filtrate.
Article
Food Science & Technology
Yuan-qing Hu, Wan-ying Wang, Turmidzi Fath, Feng-xia Li, Lin-fang Fang, Zan-hu Zhou, Dan-feng Zhang
Summary: In this study, a PMA-mPCR assay was developed for accurately screening viable vibrios in aquatic foods. The assay exhibited 100% specificity and maintained its sensitivity after PMA treatment. The results showed that the assay could detect viable V. parahaemolyticus, V. alginolyticus, and V. cholerae as low as 1.0 x 102 CFU/mL after 10 hours of enrichment. The practical application of the assay using 453 actual samples also demonstrated its accuracy in screening target vibrios in seafood.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Lisa F. Stinson, Michelle L. Trevenen, Donna T. Geddes
Summary: This study characterized the viable bacterial content and total bacterial DNA content in fresh human milk, finding that the majority of DNA recovered was from non-viable organisms. Samples treated with PMA showed significant differences in bacterial composition compared to untreated samples. These results suggest that the total bacterial DNA content in human milk does not accurately represent the viable human milk microbiome, raising questions about the validity of previous conclusions in this field.
Article
Microbiology
Lida Chen, Lei Li, Xuewen Xie, Ali Chai, Yanxia Shi, Tengfei Fan, Jianming Xie, Baoju Li
Summary: A new detection method was developed to quantify viable Fusarium cells in contaminated soil by pretreating tissue fluid with PMA and extracting DNA. The method showed high sensitivity, efficiency, and time saving.
Article
Biochemistry & Molecular Biology
Yao Liu, Caijiao Wei, Hui Wan, Sarengaowa Sarengaowa, Xiaoping Liang, Tao Jiang, Yuhe Dong, Xihong Zhao, Tian Zhong
Summary: A rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. The use of propidium monoazide (PMA) at an optimum concentration of 5.0 μg/mL successfully eliminated false positive results. The PMA-mPCR assay showed a detection limit of 10(4) CFU/mL for both pure culture and food matrix samples.
Article
Microbiology
Ya Wang, Yan Yan, Kelsey N. Thompson, Sena Bae, Emma K. Accorsi, Yancong Zhang, Jiaxian Shen, Hera Vlamakis, Erica M. Hartmann, Curtis Huttenhower
Summary: This study comprehensively evaluates PMA-seq in synthetic and complex microbial communities, finding that the technique is effective for semi-quantitative purposes in simple synthetic communities but provides only qualitative assessments in realistically complex community samples.
Article
Plant Sciences
Xuewen Xie, Lida Chen, Yanxia Shi, Ali Chai, Tengfei Fan, Lei Li, Baoju Li
Summary: This study reveals a new mode of transmission in which leaf blade disease residues in soil can spread again into the air and infect plants. Calcium cyanamide and polyethylene treatments are effective ways to block the infection of cucumber leaves by Corynespora blight residues in soil. These treatments are considered to comprise a feasible and sustainable technique for vegetable leaf residues in greenhouses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Wei Guo, Qian Yang, Yunzhe Zhang, Xin Lu, Chenchen Wu, Jianxin Tan, Wei Zhang
Summary: The aim of this study was to establish a rapid and visual nucleic acid isothermal amplification method for the detection of viable Staphylococcus aureus (S. aureus) in pork and pork products. The PMA-SRCA assay showed a higher sensitivity and reduced the false-positive detection rate of S. aureus in pork and pork products.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Haibo Zhou, Xinmei Liu, Zhaoxin Lu, Antuo Hu, Wenjie Ma, Changzheng Shi, Xiaomei Bie, Yiyu Cheng, Haijing Wu, Jun Yang
Summary: A new method called PMA-duplex ddPCR was developed for the quantitative determination of viable Vibrio parahaemolyticus in aquatic products, with lower limit of detection and high accuracy. This method allows for the detection of V. parahaemolyticus without pre-enrichment.
Article
Environmental Sciences
B. Reyneke, M. Waso, T. Ndlovu, T. Clements, B. Havenga, S. Khan, W. Khan
Summary: This study compared the effectiveness of EMA and PMA combined with ABS and qPCR assays in detecting viable bacterial species in rainwater. The results showed that both EMA and PMA reduced the detection of nonviable bacteria in rainwater samples, but EMA treatment significantly altered the relative abundance of dominant bacterial families and genera, as well as decreased the detection of unclassified bacteria. Additionally, the use of EMA and PMA increased the detection of less abundant pathogenic bacteria. The combination of viability pre-treatments with ABS and qPCR provides a comprehensive monitoring approach for detecting less abundant bacterial genera and/or pathogens in water sources.
WATER AIR AND SOIL POLLUTION
(2022)
Article
Microbiology
Aifen Ou, Kan Wang, Yanrui Ye, Ling Chen, Xiangjun Gong, Lu Qian, Junyan Liu
Summary: A method using propidium monoazide-crossing priming amplification (PMA-CPA) was developed to detect Salmonella enterica rapidly with high sensitivity and identify VBNC cells in food samples. Specific primers and testing on 20 bacterial strains confirmed the effectiveness and specificity of this method in detecting S. enterica. The successful application of PMA-CPA in detecting S. enterica in VBNC state from food samples was demonstrated with significant color change.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyung-Ho Kim, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Chan-Il Park
Summary: Red sea bream iridovirus (RSIV) is a significant aquatic virus causing high mortality in marine fish. A viability qPCR assay based on PMAxx was developed, which effectively differentiated between infectious and inactive viruses and detected infectious RSIV more efficiently than conventional methods. This method can prevent overestimation of red sea bream iridoviral disease caused by RSIV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Surangi H. H. Thilakarathna, Taryn Stokowski, Linda Chui
Summary: The vPCR protocol using PMAxx (TM) as the intercalating agent was optimized for selective amplification of live bacterial DNA. The study examined different methods of PMAxx (TM) addition, a wash step post-treatment, and a tube change before DNA extraction. The optimized protocol successfully eliminated false positives and minimized false negatives. With further improvements, vPCR has great potential as a culture-independent diagnostic tool.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Chenxia Cao, Weichen Bao, Weicheng Li, Feiyan Zhao, Lai-yu Kwok, Wenyi Zhang, Heping Zhang
Summary: Inoculating Lactobacillus plantarum PS-8 during alfalfa silage fermentation can improve silage quality by accelerating acidification, reducing harmful microorganisms, and increasing protein and organic acid levels. Tracking the dynamics of viable microbiota provides insights into their physiological contribution to silage fermentation.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kevin M. Koch, Matthew F. Koff, Thomas W. Bauer, Parina H. Shah, Andrew S. Nencka, S. Sivaram Kaushik, Hollis G. Potter
MAGNETIC RESONANCE IN MEDICINE
(2018)
Article
Medical Laboratory Technology
Andrew J. Evans, Thomas W. Bauer, Marilyn M. Bui, Toby C. Cornish, Helena Duncan, Eric F. Glassy, Jason Hipp, Robert S. McGee, Doug Murphy, Charles Myers, Dennis G. O'Neill, Anil V. Parwani, B. Alan Rampy, Mohamed E. Salama, Liron Pantanowitz
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2018)
Article
Medical Laboratory Technology
Ayoub Nahal, Crystal Mildred O. Batac, Renee J. Slaw, Thomas W. Bauer
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
(2018)
Article
Orthopedics
Jaiben George, Yaxia Zhang, Michael Jawad, Mhamad Faour, Alison K. Klika, Thomas W. Bauer, Carlos A. Higuera
JOURNAL OF ARTHROPLASTY
(2018)
Article
Orthopedics
Matthew F. Koff, Christina Esposito, Parina Shah, Mauro Miranda, Elexis Baral, Kara Fields, Thomas Bauer, Douglas E. Padgett, Timothy Wright, Hollis G. Potter
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2019)
Review
Orthopedics
Nicolas S. Piuzzi, Mitchell Ng, Morad Chughtai, Anton Khlopas, Prem N. Ramkumar, Steven F. Harwin, Michael A. Mont, Thomas W. Bauer, George F. Muschler
Article
Orthopedics
Thomas W. Bauer, Hany Bedair, Jonathan D. Creech, Carl Deirmengian, Hannah Eriksson, Yale Fillingham, Grigor Grigoryan, Noreen Hickok, Veit Krenn, Vincent Krenn, Stergios Lazarinis, Lars Lidgren, Jess Lonner, Susan Odum, Jay Shah, AliSina Shahi, Noam Shohat, Majd Tarabichi, Annette W-Dahl, Montri D. Wongworawat
JOURNAL OF ARTHROPLASTY
(2019)
Article
Orthopedics
Arash Aalirezaie, Thomas W. Bauer, Hangama Fayaz, William Griffin, Carlos A. Higuera, Veit Krenn, Vincent Krenn, Miguel Molano, Dirk-Jan Moojen, Camilo Restrepo, AliSina Shahi, Igor Shubnyakov, Scott Sporer, Aree Tanavalee, Marco Teloken, Job Diego Velazquez Moreno
JOURNAL OF ARTHROPLASTY
(2019)
Article
Orthopedics
Noam Shohat, Thomas Bauer, Martin Buttaro, Nicolaas Budhiparama, James Cashman, Craig J. Della Valle, Lorenzo Drago, Thorsten Gehrke, Luiz S. Marcelino Gomes, Karan Goswami, Nils P. Hailer, Seung Beom Han, Carlos A. Higuera, Yutaka Inaba, Jean-Yves Jenny, Per Kjaersgaard-Andersen, Mel Lee, Adolfo Llinas, Konstantinos Malizos, Michael A. Mont, Rhidian Morgan Jones, Javad Parvizi, Trisha Peel, Salvador Rivero-Boschert, John Segreti, Alex Soriano, Ricardo Sousa, Mark Spangehl, Timothy L. Tan, Rashid Tikhilov, Ibrahim Tuncay, Heinz Winkler, Eivind Witso, Marjan Wouthuyzen-Bakker, Simon Young, Xianlong Zhang, Yixin Zhou, Werner Zimmerli
JOURNAL OF ARTHROPLASTY
(2019)
Meeting Abstract
Orthopedics
S. G. Lessard, T. Pannellini, L. Chen, P. Singh, A. A. Nocon, D. Oliver, P. Mukherjee, G. Perino, M. A. Kirksey, M. F. Koff, H. G. Potter, L. B. Ivashkiv, A. B. Pernis, T. W. Bauer, T. P. Sculco, T. M. Wright, P. K. Sculco, M. Otero
OSTEOARTHRITIS AND CARTILAGE
(2019)
Article
Orthopedics
Kordo Saeed, Parham Sendi, William Arnold, Thomas W. Bauer, Debora C. Coraca-Huber, Antonia F. Chen, Hyonmin Choe, John L. Daiss, Michelle Ghert, Noreen J. Hickok, Kohei Nishitani, Bryan D. Springer, Paul Stoodley, Thomas P. Sculco, Barry D. Brause, Javad Parvizi, Alex C. McLaren, Edward M. Schwarz
Summary: Researchers have delved into the role of bacterial toxins in musculoskeletal infections, concluding that further research is necessary to understand the effects of toxins on MSKI. They have proposed a series of research directions and goals to maximize understanding of the impact of toxins on MSKI, including studying the role of toxins in infection, researching and designing antitoxins as antimicrobial agents, and designing a cocktail of antitoxins against specific pathogens.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Orthopedics
Mohammad Sherafati, Thomas W. Bauer, Hollis G. Potter, Matthew F. Koff, Kevin M. Koch
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Orthopedics
James B. Chen, Elexis C. Baral, Robert H. Hopper, James F. McDonald, Matthew F. Koff, Hollis G. Potter, Thomas W. Bauer, Charles A. Engh, Timothy M. Wright, Douglas E. Padgett
Summary: This study compared the damage patterns, MRI presentation, and histology between mobile bearing and fixed tibial inserts retrieved postmortem. The results showed that there were differences in damage modes and severity, as well as tissue reactions, between the two types of implants.
JOURNAL OF ARTHROPLASTY
(2022)
Proceedings Paper
Engineering, Biomedical
Timothy M. Wright, Matthew F. Koff, Christina I. Esposito, Douglas E. Padgett, Thomas W. Bauer, Hollis G. Potter
BEYOND THE IMPLANT: RETRIEVAL ANALYSIS METHODS FOR IMPLANT SURVEILLANCE
(2018)
Article
Surgery
Chun Hoi Yan, Carla Renata Arciola, Alex Soriano, L. Scott Levin, Thomas W. Bauer, Javad Parvizi