期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 26, 期 3, 页码 322-331出版社
JOHN WILEY & SONS INC
DOI: 10.1002/jor.20508
关键词
chondrogenesis; progenitor cells; tissue engineering
类别
资金
- NIBIB NIH HHS [EB003805] Funding Source: Medline
Bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived progenitor cells (ADPCs) are potential alternatives to autologous chondrocytes for cartilage resurfacing strategies. In this study, the chondrogenic potentials of these cell types were compared by quantifying neo-tissue synthesis and assaying gene expression and accumulation of extracellular matrix (ECM) components of cartilage. Adult equine progenitor cells encapsulated in agarose or self-assembling peptide hydrogels were cultured in the presence or absence of TGF beta 1 for 3 weeks. In BM-MSCs-seeded hydrogels, TGF beta 1 stimulated EGM synthesis and accumulation 3-41-fold relative to TGF beta 1-free culture. In ADPC cultures, TGF beta 1 stimulated a significant increase in ECM synthesis and accumulation in peptide (18-29-fold) but not agarose hydrogels. Chromatographic analysis of BM-MSC-seeded agarose and peptide hydrogels cultured in TGF beta 1 medium showed extensive synthesis of aggrecan-like proteoglycan monomers. ADPCs seeded in peptide hydrogel also synthesized aggrecan-like proteoglycans, although to a lesser extent than seen in BM-MSC hydrogels, whereas aggrecan-like proteoglycan synthesis in ADPC-seeded agarose was minimal. RT-PCR analysis of TGF beta 1 cultures showed detectable levels of type II collagen gene expression in BM-MSC but not ADPC cultures. Histological analysis of TGF beta 1-cultured peptide hydrogels showed the deposition of a continuous proteoglycan- and type II collagen rich ECM for BM-MSCs but not ADPCs. Therefore, this study showed both protein and gene expression evidence of superior chondrogenesis of BM-MSCs relative to ADPCs. (C) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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