Article
Chemistry, Applied
Dongdong Xu, Haiqi Jia, Yuqin Niu, Shouchun Yin
Summary: Heavy metal ions are toxic pollutants and detecting them is crucial for global public health. Fluorescent chemosensors based on fluorine-boron compounds have gained attention due to their ease of synthesis, high sensitivity, and modifiability. This review presents the progress in the development of these chemosensors for metal ion detection, discussing mechanisms and providing examples for the detection of specific metal ions, as well as briefly discussing other metal ions and proposing future directions.
Article
Chemistry, Applied
Peng Liao, Xianzhe Yu, Cailing Fan, Binjie Zhang, Jiarong Huang, Yadong Wu, Gengyu Du, Qinxi Dong, Chaoyuan Zeng
Summary: A novel fluorescent probe (R)-3 based on BINOL was synthesized and it could selectively detect Arg and Lys by complexing with zinc ions. The detection limit for D-/L-Arg was 60 nM and 123 nM, respectively, and the detection limit for Lys was 53 nM. Mechanistic studies revealed that the chemoselectivity comes from the selective 1,4-Michael addition to the acrylate group of the probe. A test strip for rapid and visual detection of Arg was prepared.
Review
Chemistry, Analytical
Hamed M. Al-Saidi, Sikandar Khan
Summary: This article introduces the excellent biological and non-biological applications of thioureas and their derivatives, as well as their use as chemosensors for detecting environmental pollutants and analyzing samples. The performance of thiourea-based chemosensors is compared and discussed.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Mariangela Oggianu, Cristiana Figus, Suchithra Ashoka-Sahadevan, Noemi Monni, Daniela Marongiu, Michele Saba, Andrea Mura, Giovanni Bongiovanni, Claudia Caltagirone, Vito Lippolis, Carla Cannas, Enzo Cadoni, Maria Laura Mercuri, Francesco Quochi
Summary: The potential of silicon-based fluorescent platforms for trace toxic metal ion detection in aqueous environments was investigated, showing high sensitivity and selectivity, as well as good reversibility. The architecture allows tailoring of selectivity towards other metal ions by proper fluorophore selection.
Article
Chemistry, Multidisciplinary
Changjia Hu, Junbo Chen, Peng Yang, Lijie Du, Lingying Xia, Juan He, Xiandeng Hou
Summary: In this study, we developed a monitoring scheme for nucleic acid amplification process using UiO-66-NH2. The release of pyrophosphate ion (PPi) during amplification competitively coordinates with Zr, weakening the ligand-to-metal charge transfer and causing fluorescence recovery, thus indicating the progress of amplification.
CHEMICAL COMMUNICATIONS
(2022)
Review
Chemistry, Analytical
Ning Xia, Yong Chang, Qian Zhou, Shoujie Ding, Fengli Gao
Summary: This review systematically introduces the recent advancements of MOFs-based fluorescent chemosensors and biosensors, focusing on the sensing mechanisms and analytes. It emphasizes the potential value of MOFs-based sensing platforms in environmental monitoring and clinical diagnosis.
Article
Chemistry, Inorganic & Nuclear
R. Selva Kumar, S. K. Ashok Kumar
Summary: A dual-mode chemosensor based on phenanthroline dicarbohydrazide (L) was synthesized for selective and sensitive monitoring of Zr4+. The sensor showed selective distinct colorimetric response and a turn-on fluorescence when interacting with Zr4+ in DMSO:water (8:2, v/v) media. The sensor could form 1:2 stoichiometric complex with Zr4+ and work in a wide pH range of 3 to 8, with a LOD of 180 nM and 9 nM by UV-visible and spectrofluorometric analysis respectively.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Xianzhe Yu, Binjie Zhang, Peng Liao, Jiarong Huang, Cailing Fan, Hui Hu, Qinxi Dong, Gengyu Du, Yanan Gao, Chaoyuan Zeng
Summary: Arginine is an essential amino acid with many biological significances. The chemoselective detection of Arg using fluorescent techniques is challenging. A new fluorescent probe has been synthesized for the selective detection of Arg.
Review
Chemistry, Inorganic & Nuclear
Jiaqi Xu, Rundong Jiang, Yuxin Feng, Zhuqing Liu, Jin Huang, Changbei Ma, Kemin Wang
Summary: Metal ions are ubiquitous elements in the environment and their excessive accumulation poses a threat to human health. Functional nucleic acid (FNA)-based fluorescent probes have been proposed as ideal options for the detection and imaging of various metal ions due to their high sensitivity and selectivity. This review summarizes the advancements of FNA-based fluorescent methods for metal ion sensing, focusing on the unique properties of metal ions and versatile design strategies, as well as discussing the applications of DNA-templated nanomaterials, conformation-specific fluorophores, and isothermal amplification.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Yongjie Zhang, Ning Feng, Shujin Zhou, Xia Xin
Summary: Gold nanoclusters were modified with Zn2+ and 2-methylimidazole to enhance their luminescence, resulting in nanocomposites with either blue-green emission from ZnS quantum dots or orange emission from a ZIF-8 shell, demonstrating potential applications in chemical sensing and light-converting devices.
Article
Chemistry, Multidisciplinary
Oguzhan Dalkilic, Ebru Bozkurt, Haydar Kilic
Summary: Novel hexaphenylbenzene derivatives were synthesized and their optical properties were investigated. The results showed that HPB-1 and HPB-2 can selectively sense F- ions and be used as turn-on fluorometric sensors.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Pritam Ghosh, Partha Roy
Summary: Rhodamine-based chemosensors have wide applications in detecting various metal ions in biological and environmental sciences. Their color and fluorescence properties can be regulated by the opening and closing of spirolactam ring. The selectivity of metal sensing in rhodamine dyes is influenced by factors such as solvent, pH, and interaction mode with cations. This research article discusses the correlation between structure and metal ion selectivity in rhodamine derivatives, presenting more than 140 research articles.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Applied
Qianyi Wang, Weiquan Ke, Hengyi Lou, Yihui Han, Junmin Wan
Summary: The synthesis of ZnTCPP-MOF allows for efficient detection of Pb2+ ions in water, demonstrating excellent selectivity and sensitivity. The fluorescent properties of ZnTCPP-MOF were prominent in the experiment, enabling rapid detection of Pb2+ ions even at low concentrations. The study highlights the ability of ZnTCPP-MOF to detect Pb2+ ions in water, with a low limit of detection of 4.99 x 10-8 M, which outperforms other MOFs.
Article
Chemistry, Multidisciplinary
Jiahui Zhang, Yan Jing, Peng Zhang, Benhua Xu
Summary: In this study, oxygen-doped g-C3N4 quantum dots (OCNQDs) were successfully synthesized by sintering and ultrasonic-assisted liquid-phase exfoliation methods. The OCNQDs exhibited uniform size, high crystalline quality, and excellent fluorescence properties. They showed high sensitivity and selectivity to Fe3+ ions, and were successfully applied for live cell imaging and Fe3+ ion detection with low cytotoxicity, good biocompatibility, and high permeability.
Article
Chemistry, Analytical
Yue Xin, Dun Zhang, Yan Zeng, Yingwen Wang, Peng Qi
Summary: In this research, a dual-emission ratiometric fluorescent probe Cu NCs-Al@ZIF-90 was developed for accurate and rapid detection of ATP. The probe was synthesized by encapsulating copper nanoclusters (Cu NCs) into zeolitic imidazolate framework-90 (ZIF-90) using a simple one-pot method. The probe exhibited reverse fluorescence changes at 620 nm and 450 nm in response to ATP, allowing for efficient and rapid ATP determination with a detection limit of 0.034 mM.
ANALYTICAL METHODS
(2023)
Review
Chemistry, Organic
Mireille Vonlanthen, Fabian Cuetara-Guadarrama, Pasquale Porcu, Kendra Sorroza-Martinez, Israel Gonzalez-Mendez, Ernesto Rivera
Summary: This review discusses different methods and strategies for synthesizing flexible and rigid dendronized porphyrins, with a particular focus on those reported in the last 10 years. The Lindsey methodology allows for the synthesis of porphyrins with various substitution patterns, and different synthetic methodologies have been used to prepare porphyrin dendrimers. Click chemistry reaction has been advantageous for the development of more complex porphyrin dendritic structures. The constructs presented in this review have potential applications in artificial photosynthesis, sensing, nanomedicine, and biological sciences.
CURRENT ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jenny W. Cheung, William D. Kinney, Joshua S. Wesalo, Megan Reed, Eve M. Nicholson, Alexander Deiters, T. Ashton Cropp
Summary: The use of light to control protein function is a critical tool in chemical biology. In this paper, we describe the addition of a photocaged histidine to the genetic code, allowing for the optical control of enzymes. By genetically encoding photocaged histidine in mammalian cells, we were able to successfully manipulate and control firefly and Renilla luciferases using light.
Article
Chemistry, Multidisciplinary
Wes Brown, Joshua Wesalo, Michael Tsang, Alexander Deiters
Summary: Precise temporal regulation of protein function is important for embryo development. The zebrafish embryo is an excellent model for studying development, but there is a lack of small molecule switches for protein function control. Genetic code expansion was used to incorporate unnatural amino acids with caging groups into proteins, allowing for conditional control through small molecules. This method was successfully used to activate enzymes in zebrafish embryos with temporal precision, leading to insights into heart defect development and control of DNA and protein cleavage events.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Wes Brown, Jason D. Galpin, Carolyn Rosenblum, Michael Tsang, Christopher A. Ahern, Alexander Deiters
Summary: Genetic code expansion has allowed the incorporation of unnatural amino acids into proteins. In this study, chemically acylating tRNAs were used to site-specifically incorporate unnatural amino acids into proteins in zebrafish embryos. This approach was successful in incorporating a photocaged histidine analogue that could not be achieved through enzyme engineering. Additionally, optical control over different enzymes in live embryos was demonstrated by installing photocaged histidine into their active sites.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Kristie E. Darrah, Savannah Albright, Rohan Kumbhare, Michael Tsang, James K. Chen, Alexander Deiters
Summary: The structure and mechanism of the bacterial enzyme beta-lactamase, which is clinically important for antibiotic resistance, have been extensively studied. Beta-lactamase can hydrolyze the beta-lactam ring of the cephalosporin scaffold, leading to self-immolation. This study presents a circular caged morpholino oligonucleotide (cMO) activated by beta-lactamase-mediated cleavage of a cephalosporin motif, showing potential for silencing gene expression and eliciting observable phenotypes in aquatic embryos.
ACS CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wes Brown, Joshua Wesalo, Subhas Samanta, Ji Luo, Steven E. Caldwell, Michael Tsang, Alexander Deiters
Summary: The technique of strategically placing unnatural amino acids into kinases and phosphatases' active sites has resulted in the development of photocaged signaling proteins, enabling precise light control over pathway activation. However, applying this technology to study cell signaling in embryonic development has been limited. In this study, we demonstrate light-induced activation of Protein Kinase A and a RASopathy mutant of NRAS in zebrafish embryos using a novel light-activated amino acid. This approach provides insights into the roles of these proteins in gastrulation and heart development, opening up possibilities for further investigation of RASopathy mutant proteins in animals.
ACS CHEMICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Elisa Ruffo, Adam A. Butchy, Yaniv Tivon, Victor So, Michael Kvorjak, Avani Parikh, Eric L. Adams, Natasa Miskov-Zivanov, Olivera J. Finn, Alexander Deiters, Jason Lohmueller
Summary: This study introduces engineered cell-surface receptors called chimeric antigen receptors (CARs) and synthetic Notch (synNotch) receptors, which can sense target antigens and respond accordingly. The authors develop universal receptor systems that can be post-translationally directed to specific antigens through covalent attachment of BG-conjugated antibodies. They demonstrate successful targeting of SNAP-CAR and SNAP-synNotch receptors using clinically relevant BG-conjugated antibodies, showing anti-tumor activity in a human tumor xenograft mouse model. A mathematical model is also developed to better understand the parameters affecting universal receptor signaling.
NATURE COMMUNICATIONS
(2023)
Article
Polymer Science
Ricardo D. Martinez-Serrano, Fabian Cuetara-Guadarrama, Mireille Vonlanthen, Javier Illescas, Xiao-Xia Zhu, Ernesto Rivera
Summary: Poly(ethylene glycol) methyl ether acrylate (PEGMA) fluorescent polymer hydrogels containing pyrenebutyl pendant groups were prepared using frontal polymerization (FP). The hydrogels were characterized by FT-IR spectroscopy, TGA, DSC, and swelling behavior studies. The optical properties of the hydrogels were determined by UV-visible absorption and fluorescence spectroscopies, revealing the coexistence of monomer and excimer emissions.
Article
Chemistry, Inorganic & Nuclear
Ina Hierlmeier, Amaury Guillou, Daniel F. Earley, Anthony Linden, Jason P. Holland, Mark D. Bartholomae
Summary: In this study, we synthesized and investigated the coordination chemistry of novel copper(II) and zinc(II) complexes using two heterocyclic triazacyclononane(tacn)-based chelators (HNODThia and NODThia-AcNHEt). The modified chelator HNODThia was used to develop a PSMA-based bioconjugate (NODThia-PSMA) and a photoactivatable azamacrocyclic analogue (NODThia-PEG ( 3 ) -ArN ( 3 )) for copper-64 radiopharmaceuticals. The metal-binding chelates showed rapid radiolabeling, stability in human plasma, and successful photoconjugation to albumin and antibodies. These findings have significant implications for the development of stable Cu-64-based radiopharmaceuticals and the future application of Cu-67 in targeted radioimmunotherapy.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Savannah Albright, Mary Cacace, Yaniv Tivon, Alexander Deiters
Summary: Covalent aptamers are innovative biochemical tools that can transfer labels to target proteins quickly and selectively. Equipped with cleavable electrophiles, these nucleic acid probes allow for the installation of functional handles on native proteins. Their high affinity and specificity enable covalent labeling that can compete with nuclease-mediated degradation. In this study, we successfully applied covalent aptamers to modify a specific cell surface protein, protein tyrosine kinase 7 (PTK7), by transferring biotin to specific lysine residues on its extracellular domain. This enabled us to track PTK7 expression, localization, and cellular internalization. These findings validate the programmability of covalent aptamers and demonstrate their applicability in a cellular context for protein and small molecule delivery.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Organic
Brittany M. Klootwyk, Amy E. Ryan, Arbil Lopez, Mitchell J. R. McCloskey, Chasity P. Janosko, Alexander Deiters, Paul E. Floreancig
Summary: Phosphate mono- and diesters can be efficiently released from boryl allyloxy (BAO) and related phosphotriesters by H2O2. This method was successfully applied to release a phosphorylated serine derivative and the nucleotide analogue AZT monophosphate. The release of nucleotides in the presence of ATP and a kinase leads to the formation of diphosphate, demonstrating the potential application of this method in biological processes.
Article
Biochemical Research Methods
Michael Kvorjak, Elisa Ruffo, Yaniv Tivon, Victor So, Avani Parikh, Alexander Deiters, Jason Lohmueller
Summary: Engineered T cell therapies as living drugs have unique functional capabilities that revolutionize disease treatment. However, they have limitations such as unpredictable behavior, toxicities, and nontraditional pharmacokinetics. To address these issues, we have developed OFF-switch adaptors that can conditionally control CAR T cell activity, enhancing the programmability and potential safety of universal CAR T cells.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Chasity P. Janosko, Olivia Shade, Taylor M. Courtney, Trevor J. Horst, Melinda Liu, Sagar D. Khare, Alexander Deiters
Summary: An arylazopyrazole was studied as an enhanced photoswitchable amino acid for genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in bacterial and mammalian cells. Although pulsed irradiations were required for photocontrol of translation, complete selectivity for the trans-configuration was achieved by the pyrrolysyl tRNA synthetase, demonstrating the selective control of gene expression through light exposure.
Article
Chemistry, Organic
Steven E. Caldwell, Chasity P. Janosko, Alexander Deiters
Summary: In this study, a light-activated STING agonist was developed to selectively activate the STING pathway by blocking a key interaction. This finding expands the toolbox of conditionally controlled STING agonists and provides a potential solution to avoid systemic immune activation.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wenyuan Zhou, Amy Ryan, Chasity P. Janosko, Karsen E. Shoger, Jason M. Haugh, Rachel A. Gottschalk, Alexander Deiters
Summary: Evolution has diversified the mammalian proteome through the generation of protein isoforms, but it is difficult to differentiate between these isoforms in cellular contexts. This study presents a method for the precise light control of individual isoforms of the mitogen-activated protein kinase (MAPK) p38, revealing a novel point of crosstalk between two MAPK pathways.
RSC CHEMICAL BIOLOGY
(2023)