Article
Biochemistry & Molecular Biology
Anurag Noonikara-Poyil, Alvaro Munoz-Castro, H. V. Rasika Dias
Summary: This study describes the isolation and full characterization of a thermally stable, copper(I) acetylene complex using a highly fluorinated bis(pyrazolyl)borate ligand support, as well as the details of a related copper(I) complex. Raman data show significant red-shifts in C equivalent to C stretch of different alkynes bound to copper(I), with computational analysis indicating that the interaction is primarily of the electrostatic character. The study also highlights the catalytic activity of a mononuclear copper complex in [3 + 2] cycloadditions and compares its effectiveness with a trinuclear copper catalyst.
Article
Nanoscience & Nanotechnology
Michael J. Giannetto, Eric P. Johnson, Adam Watson, Edgar Dimitrov, Andrew Kurth, Wenbo Shi, Francesco Fornasiero, Eric R. Meshot, Desiree L. Plata
Summary: Current research provides experimental evidence that short-chained alkynes can directly incorporate into the lattice of carbon nanotubes during growth, affecting their morphology and structure. Different feedstock hydrocarbons result in distinct morphological differences and nanoscale alignment of the nanotubes. These findings have important implications for exploring the structure and properties of carbon nanotubes.
ACS NANOSCIENCE AU
(2023)
Article
Chemistry, Multidisciplinary
Jashuva V. P. Katuri, Kuppuswamy Nagarajan
Summary: A simple, efficient, and scalable one-pot procedure for synthesizing N-protected beta-cyano L-alanine tertiary butyl esters from N-protected L-asparagines was reported. The method provides good yields without the need for column chromatography. Additionally, nitrile containing dipeptides were successfully synthesized by coupling N-Boc-beta-cyano L-alanine with amino acid methyl ester hydrochlorides.
Article
Chemistry, Multidisciplinary
Ioana Nicolau, Anca Paun, Codruta C. Popescu, Niculina D. D. Hadade, Mihaela Matache
Summary: This communication presents an updated experiment of peptide synthesis, involving the determination of loading capacity of a Fmoc-protected amino functionalized resin and subsequent synthesis of a dipeptide. The experiment highlights the importance of solid-phase peptide synthesis (SPPS) in accessing proteins or small-molecule peptide-based chemical probes. It combines skills in organic and analytical chemistry, requiring knowledge in amino acid reactivity, protecting groups, UV-vis spectrophotometric determinations, and mass spectrometry.
JOURNAL OF CHEMICAL EDUCATION
(2023)
Article
Microbiology
Dominik Wuellner, Maren Gesper, Annika Haupt, Xiaofei Liang, Pei Zhou, Pascal Dietze, Franz Narberhaus, Julia E. Bandow
Summary: This study investigated the physiological response of Pseudomonas aeruginosa to various antibiotics, revealing cellular defense strategies and similarities in response profiles. The findings provide insights into the effects of commonly used antibiotics on P. aeruginosa and contribute to the evaluation of novel compounds.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biochemistry & Molecular Biology
Jordi F. Keijzer, Bauke Albada
Summary: This review discusses the use of DNA in protein modification to achieve concentration of reactants, selectivity, and enhancement of reactions.
Article
Chemistry, Multidisciplinary
Daniel Obitz, Karmel S. Gkika, Marvin Heller, Tia E. Keyes, Nils Metzler-Nolte
Summary: A lanthanide(iii) complex connected to a light harvesting antenna was synthesized and tested as a potential photosensitizer in photodynamic therapy. The complex showed significant phototoxic activity on cancer cells upon irradiation, with ROS production identified as the compound's phototoxic mode of action. In cell viability assays, a 10-fold decrease in IC50 value was observed compared to the dark control.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Marion Graf, Jasmine Ochs, Nils Metzler-Nolte, Peter Mayer, Hans-Christian Bottcher
Summary: This paper describes the synthesis and characterization of six new organometallic half-sandwich iridium(III) compounds with modified bipyridine ligands. The cytotoxic properties of the compounds against two cancer cell lines were evaluated, and compound 3 showed the most remarkable biological activity with significantly enhanced toxicity compared to cisplatin.
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
(2023)
Article
Chemistry, Multidisciplinary
Paramita Sarkar, Kathakali De, Malvika Modi, Geetika Dhanda, Richa Priyadarshini, Julia E. Bandow, Jayanta Haldar
Summary: Resistance to vancomycin requires developing alternative therapeutics. In this study, vancomycin derivatives with mechanisms beyond d-Ala-d-Ala binding were reported. The lead molecule, VanQAmC(10), inhibits bacterial cell division, which is a previously unknown property for glycopeptide antibiotics.
Article
Biochemical Research Methods
Boudewijn Hollebrands, Jos A. Hageman, Jasper W. van de Sande, Bauke Albada, Hans-Gerd Janssen
Summary: Peptides play a significant role in determining the taste of food products, with their taste impressions depending on the amino acids present and their sequence. Identifying short peptides in foods is challenging, but a method was developed to accurately predict retention times of short peptides using reversed phase separation. By training a model using a combination of peptide descriptors and a newly derived set of amino acid index derived sequence-specific peptide descriptors, the model achieved a high accuracy in predicting retention times and elution order for 93% of the short peptides.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Bauke Albada
Summary: Bioconjugation of chemical entities to biologically active proteins has provided valuable insights into cellular processes and yielded innovative therapeutic agents. However, a current challenge lies in efficiently generating uniform conjugates of native proteins in their natural environment. By combining various features of protein-modifying enzymes, artificial constructs have been developed. This review evaluates the current status of this approach, with a focus on the protein-binding anchor, modification chemistry, linker, and suggests the incorporation of trigger-responsive switches for regulating protein modification.
Article
Chemistry, Multidisciplinary
Johannes A. M. Damen, Jorge Escorihuela, Han Zuilhof, Floris L. van Delft, Bauke Albada
Summary: The rates of reaction between strained cycloalkynes and cycloalkenes with 1,2-quinone were studied using stopped flow UV-Vis spectroscopy and computational analysis. The results showed that strained alkyne BCN OH 3 reacted over 150 times faster than strained alkene TCO-OH 5, and the introduction of a carbamate derivative reduced the rate constant by almost half. Additionally, the 8-membered strained alkyne BCN OH 3 reacted 16 times faster than the more strained 7-membered THS 2. Thermodynamic activation parameters were determined using the linearized Eyring equation, providing valuable insights into these rapid click reactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Medicinal
Irina Kollmorgen, Nathalie Bachmann, Michael Dal Molin, Carsten Degenhart, Eldar Zent, Vikram Pareek, Uwe Koch, Jan Rybniker, Nils Metzler-Nolte, Raphael Stoll, Bert Klebl, Julia Elisabeth Bandow, Jurgen Scherkenbeck
Summary: Due to increasing resistances, there is a need for antibacterial compounds with unique modes of action. One such compound, moiramide B, shows strong activity against gram-positive bacteria but weaker activity against gram-negative bacteria. The structure-activity relationship of moiramide B presents a challenge for optimization strategies, while the fatty acid tail is considered as a transport vehicle. However, the sorbic acid unit is found to be relevant for ACC inhibition and allows the development of moiramide derivatives with altered antibacterial profiles.
Article
Chemistry, Organic
Jasper W. van de Sande, Bauke Albada
Summary: The researchers reported the total synthesis of post-translationally modified peptides, including beta-1,2-linked L-arabinosylated Fmoc-protected hydroxyproline building blocks and sulfated tyrosine and hydroxyproline, which are important regulating molecules for living organisms. Clean glycopeptides were obtained by removing acetyl groups from the L-arabinose and neopentyl-protected sulfated tyrosine before deprotection.
Article
Biochemical Research Methods
Melissa Vazquez-Hernandez, Stephanie L. Leedom, Kenneth C. Keiler, Julia Elisabeth Bandow
Summary: Trans-translation is the most effective ribosome rescue system in bacteria, but Bacillus subtilis possesses two alternative ribosome rescue mechanisms. In this study, the proteomes of B. subtilis 168 and a ssrA mutant were compared using quantitative proteomics. The mutant strain showed lower growth rate and protein synthesis rates, altered motility and chemotaxis phenotype, and alterations in the abundance of ribosomal proteins and proteins related to amino acid biosynthesis, reflecting slow ribosome recycling.
Article
Infectious Diseases
Leonie Sagurna, Sascha Heinrich, Lara-Sophie Kaufmann, Christian Rueckert-Reed, Tobias Busche, Alexander Wolf, Jan Eickhoff, Bert Klebl, Joern Kalinowski, Julia E. Bandow
Summary: Bacteria of the genus Streptomyces produce various specialized metabolites. Single biosynthetic gene clusters (BGCs) can give rise to different products with varying biological activities. In this study, alnumycin and 1,6-dihydro 8-propylanthraquinone were isolated from a Streptomyces strain containing the alnumycin BGC. Both compounds exhibited antimicrobial activity, with 1,6-dihydro 8-propylanthraquinone showing higher activity against E. coli & UDelta;tolC compared to alnumycin. The modes of action of these compounds are worth further investigation.
Review
Biochemistry & Molecular Biology
Jaccoline Zegers, Maartje Peters, Bauke Albada
Summary: This article summarizes the importance of stabilizing guanine quadruplexes (G4s) for cancer treatment and explores the methods and techniques for stabilizing G4s using metal complexes. By analyzing the G4-stabilizing capacity and bioactivity data of 74 metal-based ligands, guidelines for future studies in this field are proposed.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tim Dirks, Marco Krewing, Katharina Vogel, Julia E. Bandow
Summary: Cold atmospheric pressure plasmas have a significant impact on protein aggregation, and Hsp33 plays an important role in preventing protein aggregation. It has been found that over-production of Hsp33 can increase bacterial resistance to cold plasmas.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Tim Dirks, Abdulkadir Yayci, Sabrina Klopsch, Marco Krewing, Wuyuan Zhang, Frank Hollmann, Julia E. Bandow
Summary: This study demonstrates that immobilization universally protects enzymes under plasma-operating conditions and improves their activity, opening up new possibilities for emerging applications.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
