Article
Microbiology
Can-Hua Lu, Ying-Ying Zhang, Ning Jiang, Wei Chen, Xiaoli Shao, Zhi-Ming Zhao, Wen-Lin Lu, Xiaodong Hu, Yi-Xuan Xi, Si-Yuan Zou, Qiu-Ju Wei, Zhong-Long Lin, Li Gong, Xiao-Tong Gai, Li-Qun Zhang, Jun-Ying Li, Yan Jin, Zhen-Yuan Xia
Summary: Eight Gram-negative, aerobic, motile with paired polar flagella and rod-shaped bacteria from tobacco fields in Yunnan, PR China were identified as Ralstonia genus. The strains showed high similarity to R. wenshanensis, R. pickettii, and R. insidiosa. Genome sequencing revealed the presence of new species within the genus Ralstonia. Based on the data, three new species names were proposed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Suchi Parvin Biki, Shobuz Mahmud, Sumaia Akhter, Md Jiaur Rahman, Jessica J. Rix, Md Adnan Al Bachchu, Maruf Ahmed
Summary: In this study, bacteria were separated from landfill soil and their ability to degrade LDPE was evaluated. Ralstonia sp. strain SKM2 and Bacillus sp. strain SM1 were identified as effective LDPE-degrading microorganisms, causing weight loss and pH changes in the media. Structural changes in LDPE sheet were observed under microscope and FTIR analysis showed alterations in carbon bonds and groups.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Engineering, Environmental
Tianyuan Gu, Wenjing Niu, Yongming Wu, Lili Huo, Zulfiqar Ahmad, Mi Deng, Hua Zhong
Summary: Molasses has potential for both chemical reduction and bioreduction of Cr(VI) in groundwater. Low concentration of molasses can significantly enhance bioreduction of Cr(VI) and outperforms sucrose. Bacillus sp., isolated from the site soils, can reduce Cr(VI) using molasses as the sole carbon source.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Engineering, Environmental
Xitong Wang, Hao Li, Huayan Huang, Huanyan Luo, Shihua Luo, Lili Jiang, Su Feng, Heng Xu
Summary: The study found that strain TH-1, a Cr(VI) reducing bacterium, had great potential to efficiently remove Cr(VI) from contaminated sites under different environmental conditions. The optimal growth conditions and reduction mechanisms of the strain were identified, highlighting its significance for the remediation of Cr(VI) contaminated environmental sites.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Environmental Sciences
Jia Chen, Xiaoguang Li, Longzhan Gan, Guangyang Jiang, Ruoshi Zhang, Zhe Xu, Yongqiang Tian
Summary: The isolated strain Lysinibacillus HST-98 shows promising results in reducing Cr(VI) pollution, even at high concentrations. Optimal growth conditions for the strain include a pH of 8-9, a temperature of 36 degrees Celsius, and sodium lactate as an electron donor. Coexisting metal ions like Cu2+, Co2+, and Mn2+ enhance Cr(VI) reduction, while Zn2+, Ni2+, and Cd2+ have the opposite effect. The reduction mechanism of strain HST-98 is primarily mediated by intracellular enzymes, and the genes and enzymes related to chromium metabolism in this strain have been annotated to further understand its mechanism.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Kilian Colas, A. Catarina V. D. dos Santos, Stefanie Kohlhepp, Abraham Mendoza
Summary: The addition of organometallic reagents to aliphatic carboxylic acids for the synthesis of ketones is limited to organolithium reagents, but can be extended to Grignard reagents using a bulky aniline-derived turbo-Hauser base. This method allows for the straightforward preparation of a variety of aliphatic and perfluoroalkyl ketones.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Renata Kolodziejska, Renata Studzinska, Agnieszka Tafelska-Kaczmarek, Hanna Pawluk, Dominika Mlicka, Alina Wozniak
Summary: This study utilized the bioreduction capabilities of Aureobasidium pullulans strains DSM 14940 and DSM 14941 to successfully synthesize high enantiomeric purity of (S)-benzoin. Mild conditions were employed for benzoin synthesis using two different methodologies, and enrichment of (R)-benzoin was achieved through changes in reaction conditions and additives.
Article
Horticulture
Rachidatou Sikirou, Marie Epiphane Dossoumou, Judith Honfoga, Victor Afari-Sefa, Ramasamy Srinivasan, Mathews Paret, Wubetu Bihon
Summary: Bacterial wilt caused by Ralstonia solanacearum is a growing concern in amaranth production in Benin. Host resistance is identified as the most sustainable disease control measure. The study suggests that the UG-AMES13-2 variety from the World Vegetable Center shows high resistance to R. solanacearum and should be promoted for seed production to support farmers.
Article
Chemistry, Inorganic & Nuclear
Himani Ahuja, Harleen Kaur, Rebeca Arevalo
Summary: The manganese(ii) complex [Mn(SiNSi)Cl2] is an efficient catalyst for the chemoselective C(sp)-H borylation of terminal alkynes.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Microbiology
Can-Hua Lu, Wei Chen, Hong -Hui Yin, Zhong-Long Lin, Jun-Ying Li, Xiao-Tong Gai, Ning Jiang, Zheng-Hua Cao, Lei Qian, Li-Qun Zhang, Yan Jin, Zhen- Yuan Xia
Summary: A new bacterium strain, named Ralstonia wenshanensis sp. nov., was isolated from tobacco planting soil in Yunnan, PR China. It shares similarities with other members of the genus Ralstonia in terms of physiological characteristics and gene sequences, but also exhibits distinct differences.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Doeun Jeon, Lingmin Jiang, Ki-Hyun Kim, Yuxin Peng, Donghyun Cho, Rae-Dong Jeong, Cha Young Kim, Jae Cheol Jeong, Jiyoung Lee
Summary: A new rod-shaped bacterium strain DM-R-R2A-13(T), named Massilia endophytica sp. nov., was isolated from the plant Cannabis sativa L. 'Cheungsam'. The strain has the potential to produce the bioplastic poly-3-hydroxybutyrate (PHB).
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Inorganic & Nuclear
Mahsa Zarei, Faezeh Fatemi, Razieh Ghasemi, Mohammad Mir-Derikvand, Hamzeh Hosseinpour, Touran Rabiee Samani
Summary: The native strain Shewanella sp. RCRI7 can reduce uranium in anaerobic conditions. This study investigated the mutual effects of uranium and the bacteria and found that uranium reduced the population of nonanaerobicized bacteria and affected the growth and development of the anaerobicized bacteria. The study also revealed that creamy-colored Shewanella can remove soluble uranium, but bacteria with red cytochromes are the most effective.
APPLIED RADIATION AND ISOTOPES
(2023)
Article
Chemistry, Multidisciplinary
Baoqi Zhang, Jiale Sun, Yanqiu Zheng, Xinlei Mao, Jinping Lin, Dongzhi Wei
Summary: This study reports the heterologous expression of a novel FMN-binding ene reductase (PaER) from Pichia angusta and its excellent catalytic performance in the asymmetric reduction reaction, especially for the selective reduction of ketoisophorone. Through the engineering of E. coli expression system, efficient asymmetric reduction of ketoisophorone was achieved.
Article
Chemistry, Organic
Kaori Ando, Haruka Watanabe, Xiaoxian Zhu
Summary: (E)-alpha,beta-Unsaturated aldehydes were synthesized by the Julia-Kocienski reaction of 2,2-dimethoxyethyl 1-phenyl-1H-tetrazol-5-yl (PT) sulfone 3 with various aldehydes, followed by acid hydrolysis. The reaction could be carried out in one pot, and various (E)-alpha,beta-unsaturated aldehydes were obtained in a short time and with high yields.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Plant Sciences
Si Wang, Ming Hu, Huilin Chen, Chuhao Li, Yang Xue, Xinyue Song, Yuqing Qi, Fan Liu, Xiaofan Zhou, Lian-hui Zhang, Jianuan Zhou
Summary: Two Pseudomonas strains, Q1-7 and Q4-3, capable of degrading 3-OH PAME were isolated and identified. They exhibited significant inhibition effects on Ralstonia solanacearum and effectively protected Casuarina equisetifolia, peanut and tomato plants from infection. These findings provide potential resources for the prevention and control of bacterial wilt caused by Ralstonia solanacearum.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Sergio Gonzalez-Granda, Jesus Albarran-Velo, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The combination of metal-, photo-, enzyme-, and/or organocatalysis is a versatile approach in synthetic chemistry, particularly for the creation of chiral centers. This review focuses on the recent developments in the simultaneous use of enzymes and transition metal catalysts, as well as the integration of multiple bioprocesses and metal-catalyzed transformations in linear cascades. Challenges such as catalyst compatibility, reaction media, and cross-reactivities are addressed by strategies such as catalyst coimmobilization, compartmentalization, and flow chemistry. The review also highlights the application of bionanohybrid materials, where enzymes and metals are coimmobilized on suitable supports.
Article
Chemistry, Applied
Philipp Petermeier, Christoph Kohlfuerst, Ana Torvisco, Roland C. C. Fischer, Alejandro Mata, Doris Dallinger, C. Oliver Kappe, Joerg H. H. Schrittwieser, Wolfgang Kroutil
Summary: Substituted piperidine rings are efficiently prepared with multiple stereocentres using multi-enzymatic and chemo-enzymatic methods from achiral diketoester precursors. Highly enantioselective transamination and diastereoselective reduction processes enable the synthesis of optically pure piperidines. This approach offers a more streamlined and efficient route for the synthesis of trisubstituted piperidines with potential applications in drug discovery.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Applied
P. Petermeier, C. Kohlfuerst, A. Torvisco, R. C. Fischer, A. Mata, D. Dallinger, C. O. Kappe, J. H. Schrittwieser, W. Kroutil
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Applied
Mathias Pickl, Markus Ebner, Samantha Gittings, Pere Clapes, Wolfgang Kroutil
Summary: Although optical pure amino alcohols are highly demanded, their synthesis remains challenging due to the requirement of elaborate protection strategies. This study presents a multi-enzymatic methodology that allows the synthesis of enantioenriched 1,3-amino alcohols from commodity chemicals. By utilizing a Type I aldolase and enzymatic transamination, both diastereomers of the 1,3-amino alcohol motif were accessible, resulting in the desired amino alcohols with high enantioselectivity.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Applied
Laura Rodriguez-Fernandez, Jesus Albarran-Velo, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The photocatalytic Meerwein arylation between aromatic diazonium salts and isopropenyl acetate in aqueous medium was studied under blue LED light irradiation. Optimization of reaction conditions allowed the synthesis of various 1-arylpropan-2-ones with up to 95% yield using [Acr-Mes]ClO4 as the photocatalyst. Furthermore, a one-pot sequential photobiocatalytic approach combining the Meerwein arylation with bioreduction of ketone intermediates was developed, leading to the synthesis of 1-arylpropan-2-ol enantiomers with up to 76% overall yield and high stereoselectivity.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Applied
Sergio Gonzalez-Granda, Georg Steinkellner, Karl Gruber, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The combination of gold(I) and enzyme catalysis allows for the selective synthesis of a series of nor(pseudo)ephedrine derivatives. This approach involves the hydration of 1-phenylprop-2-yn-1-yl acetate or N-(1-phenylprop-2-yn-1-yl)acetamide using IPrAuNTf2 catalyst, followed by (dynamic) asymmetric biotransamination or bioreduction of the resulting keto ester or keto amide intermediates. Enzyme actions exhibit high stereoselectivity towards methyl ketones, enabling the synthesis of enantio- and diastereomerically enriched products. The use of propargyl esters or amides as starting materials allows for the production of amino alcohol, diol, and diamine derivatives with good yields and high (stereo)selectivity.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Review
Chemistry, Multidisciplinary
Sergio Gonzalez-Granda, Lorena Escot, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The combination of metal and enzyme catalysis has attracted great attention in organic synthesis due to its ability to achieve diverse organic molecules with the stereospecificity displayed by enzymes. This minireview focuses on the action of metal species (Pd, Ru, Au, Ir, Fe, etc.) in combination with different enzymes, highlighting the design of sequential processes and concurrent cascades to overcome incompatibilities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Nicoletta Cascelli, Vincenzo Lettera, Giovanni Sannia, Vicente Gotor-Fernandez, Ivan Lavandera
Summary: Laccases are shown to be valuable in biocatalysis for the green and selective oxidation of furfuryl alcohol into furfural using mediators. Various parameters including pH, enzyme/mediator composition, buffer type, cosolvent tolerance, and reaction times are investigated. Under optimal conditions, quantitative production of furfural is achieved after 16 h using 20 mol % of TEMPO as mediator and 5.8 U mL(-1) of POXC and POXA1b laccases from Pleurotus ostreatus. POXC exhibits remarkable catalytic ability at pH 6.5, while POXA1b is notable for its high stability. Furfural conversions of up to 95% are obtained after 72 h using only 5 mol % of TEMPO at 100 mM. Furthermore, furfuryl alcohol bioamination is successfully achieved through a one-pot sequential approach using amine transaminase from Chromobacterium violaceum, providing furfuryl amine, a key compound for the polymer industry.
Review
Chemistry, Physical
Veronique Alphand, Willem J. H. van Berkel, Valentina Jurkas, Selin Kara, Robert Kourist, Wolfgang Kroutil, Francesco Mascia, Marc M. Nowaczyk, Caroline E. Paul, Sandy Schmidt, Jelena Spasic, Paula Tamagnini, Christoph K. Winkler
Summary: The recent increase of interest in photocatalysis has expanded to biocatalysis and led to a surge in the development of light-dependent enzyme-mediated or enzyme-coupled processes. Reviewing the progress in photobiocatalysis, we suggest categorizing it into different disciplines and providing guidelines for reporting research results and performing photobiocatalytic reactions. Overall, we believe that this field enhances the diversity of biocatalytic reactions and harnesses the selectivity of enzymes in photocatalysis. We anticipate that the ongoing enthusiasm for light-dependent enzymatic processes will uncover new photobiocatalytic mechanisms, complementing biocatalysis with novel bond-forming reactions and innovative strategies for utilizing light as a sustainable energy source.
Article
Biochemistry & Molecular Biology
Antia Pintor, Nicoletta Cascelli, Alexey Volkov, Vicente Gotor-Fernandez, Ivan Lavandera
Summary: This study discloses the biocatalytic amination of furan-based compounds using amine transaminases (ATAs) and isopropylamine (IPA) as amine donors. Optimal biocatalysts were identified and high levels of product formation were achieved. pH was found as a key factor affecting the transformation.
Article
Chemistry, Multidisciplinary
Antia Pintor, Ivan Lavandera, Alexey Volkov, Vicente Gotor-Fernandez
Summary: The study focused on finding an ideal biocatalyst for the acylation of furfurylamine and 5-hydroxymethylfurfurylamine (HMFA), and identified immobilized Candida antarctica lipase B (CALB) as a suitable option. CALB was immobilized on different supports and the use of a polymer-coated controlled porosity glass carrier material yielded an active and stable enzymatic preparation for the acylation reaction. By controlling the amount of acyl donor in the reaction, selective modification of the amine group of HMFA was achieved, resulting in the formation of hydroxy amides. The reaction conditions and substrate concentration were optimized to achieve high conversions and yields. The study also demonstrated the possibility of obtaining orthogonally protected HMFA-derived amido esters through a one-pot process.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Aleksandra Rudzka, Beata Zdun, Natalia Antos, Lia Martinez Montero, Tamara Reiter, Wolfgang Kroutil, Pawel Borowiecki
Summary: There is a need for biocatalysts capable of transferring hydrogen to prepare optically pure alcohols, especially for sterically demanding ketones. This study describes the biocatalytic potential of an anti-Prelog (R)-specific variant of Lactobacillus kefir ADH as a whole-cell biocatalyst for the reduction of prochiral carbonyl substrates. The results show that the biocatalyst exhibits activity towards a variety of ketones, producing optically active alcohol products with high conversion and excellent stereoselectivity.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Christopher Grimm, Simona Pompei, Kristina Egger, Michael Fuchs, Wolfgang Kroutil
Summary: Lignin-derived aryl methyl ethers have potential as carbon sources in chemistry. An alternative method for their demethylation is through cobalamin-dependent enzymes, which require a methyltransferase and a carrier protein. Fusion proteins, connecting the methyltransferase with the corrinoid-binding protein, showed better performance than separate proteins, enabling a simpler protocol for demethylation. The fusion enzyme has promising applications in diversifying natural products and valorizing lignin.
Article
Chemistry, Physical
Willem B. Breukelaar, Nakia Polidori, Amit Singh, Bastian Daniel, Silvia M. Glueck, Karl Gruber, Wolfgang Kroutil
Summary: The biocatalytic reduction of oxime to amine has been observed in ene-reductases transforming alpha-oximo beta-keto esters. Through structural analysis, molecular dynamics simulations, biocatalytic cascades, and investigation of possible intermediates, it has been determined that the reaction proceeds via an imine intermediate and not through a hydroxylamine intermediate. A non-canonical tyrosine residue in ene-reductase OPR3 was found to play a role in protonating the hydroxyl group of the oxime in the first reduction step.
Article
Chemistry, Multidisciplinary
Emmanuel Cigan, Jakob Pletz, Sarah A. Berger, Bettina Hierzberger, Michael Grilec-Zlamal, Alexander Steiner, Isabel Oroz-Guinea, Wolfgang Kroutil
Summary: This study demonstrates an efficient access to the morphinan scaffold using a combination of organic synthesis and enzymatic reactions. The synthesis of a medicinally important compound class, promorphinans, was achieved by minimizing protecting group techniques and utilizing biocatalysis.
