期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 24, 期 6, 页码 1070-1077出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2012.08.005
关键词
Resveratrol; Methionine sulfoxide reductases A; Parkinson's disease; 1-methyl-4-phenyl-pyridinium; Oxidative stress
资金
- Key Project of NSFC [30930104]
- International Science& Technology Cooperation Program of China [2011DFA32670]
- Important National Science & Technology Specific Projects [2012ZX09103-101-045]
Methionine sulfoxide reductases A (MsrA) has been postulated to act as a catalytic antioxidant system involved in the protection of oxidative stress-induced cell injury. Recently, attention has turned to MsrA in coupling with the pathology of Parkinson's disease, which is closely related to neurotoxins that cause dopaminergic neuron degeneration. Here, we firstly provided evidence that pretreatment with a natural polyphenol resveratrol (RSV) up-regulated the expression of MsrA in human neuroblastoma SH-SY5Y cells. It was also observed that the expression and nuclear translocation of forkhead box group O 3a (FOXO3a), a transcription factor that activates the human MsrA promoter, increased after RSV pretreatment. Nicotinamide, an inhibitor of silent information regulator 1 (SIRT1), prevented RSV-induced elevation of FOXO3a and MsrA expression, indicating that the effect of RSV was mediated by a SIRT1-dependent pathway. RSV preconditioning increased methionine sulfoxide(MetO)-reducing activity in SH-SY5Y cells and enhanced their resistance to neurotoxins, including chloramine-T and 1-methyl-4-phenyl-pyridinium. In addition, the enhancement of cell resistance to neurotoxins caused by RSV preconditioning can be largely prevented by MsrA inhibitor dimethyl sulfoxide. Our findings suggest that treatment with polyphenols such as RSV can be used as a potential regulatory strategy for MsrA expression and function. (C) 2013 Elsevier Inc. All rights reserved.
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