期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 22, 期 11, 页码 1047-1054出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2010.09.002
关键词
Desaturase; Elongase; beta-oxidation; Piglet
资金
- National Research Initiative [2005 35200-16174, 2007-35206-17897]
- USDA National Institute for Food and Agriculture
Effects of dietary conjugated linoleic acid (CLA, 1% mixed isomers) on n-6 long-chain polyunsaturated fatty acid (LCPUFA) oxidation and biosynthesis were investigated in liver and brain tissues of neonatal piglets. Fatty acid beta-oxidation was measured in tissue homogenates using [1-C-14]linoleic acid (LA) and -arachidonic acid (ARA) substrates, while fatty acid desaturation and elongation were traced using [U-C-13]LA and GC-MS. Dietary CLA had no effect on fatty acid beta-oxidation, but significantly decreased n-6 LCPUFA biosynthesis by inhibition of LA elongation and desaturation. Differences were noted between our C-13 tracer assessment of desaturation/elongation and simple precursor-product indices computed from fatty acid composition data, indicating that caution should be exercised when employing the later. The inhibitory effects of CLA on elongation/desaturation were more pronounced in pigs fed a low fat diet (3% fat) than a high fat diet (25% fat). Direct elongation of linoleic acid to C20:2n-6 via the alternate elongation pathway might play an important role in n-6 LCPUFA synthesis because more than 40% of the synthetic products of [U-C-13]LA accumulated in [C-13]20:2n-6. Overall, the data show that dietary CLA shifted the distribution of the synthetic products of [U-C-13]LA between elongation and desaturation in liver and decreased the total synthetic products of [U-C-13]LA in brain by inhibiting LA elongation to C20:2n-6. The impact of CIA on brain LCPUFA metabolism of the developing neonate merits consideration and further investigation. (C) 2011 Elsevier Inc. All rights reserved.
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