期刊
JOURNAL OF NUTRITION
卷 145, 期 2, 页码 207-214出版社
OXFORD UNIV PRESS
DOI: 10.3945/jn.114.199604
关键词
muscle protein synthesis; nutrition; essential amino acids; skeletal muscle; blood flow; anabolic signaling; muscle-full
资金
- Ajinomoto, Inc., Tokyo, Japan
- Medical Research Council [MR/K00414X/1] Funding Source: researchfish
- MRC [MR/K00414X/1] Funding Source: UKRI
Background: The anabolic response of skeletal muscle to essential amino acids (EAAs) is dose dependent, maximal at modest doses, and short lived, even with continued EAA availability, a phenomenon termed muscle-full. However, the effect of EAA ingestion profile on muscle metabolism remains undefined. Objective: We determined the effect of Bolus vs. Spread EAA feeding in young men and hypothesized that muscle-full is regulated by a dose-, not delivery profile-, dependent mechanism. Methods: We provided 16 young healthy men with 15 g mixed-EAA, either as a single dose (Bolus; n = 8) or in 4 fractions at 45-min intervals (Spread; n = 8). Plasma insulin and EAA concentrations were assayed by ELISA and ion-exchange chromatography, respectively. Limb blood flow by was determined by Doppler ultrasound, muscle microvascular flow by Sonovue (Bracco) contrast-enhanced ultrasound, and phosphorylation of mammalian target of rapamycin complex 1 substrates by immunoblotting. Intermittent muscle biopsies were taken to quantify myofibrillar-bound C-13(6)-phenylalanine to determine muscle protein synthesis (MPS). Results: Bolus feeding achieved rapid insulinemia (13.6 mu IU . mL(-1), 25 min after commencement of feeding), aminoacidemia (similar to 2500 mu M at 45 min), and capillary recruitment (+45% at 45 min), whereas Spread feeding achieved attenuated insulin responses, gradual low-amplitude aminoacidemia (peak: similar to 1500 mu M at 135 min), and no detectable capillary recruitment (all P < 0.01 vs. Bolus). Despite these differences, identical anabolic responses were observed; fasting fractional synthetic rates of 0.054% . h(-1) (Bolus) and 0.066% . h(-1) (Spread) increased to 0.095% and 0.104% . h(-1) (no difference in increment or final values between regimens). With both Spread and Bolus feeding strategies, a latency of at least 90 min was observed before an upswing in MPS was evident. Similarly with both feeding strategies, MPS returned to fasting rates by 180 min despite elevated circulating EAAs. Conclusion: These data do not support EAA delivery profile as an important determinant of anabolism in young men at rest, nor rapid aminoacidemia/leucinemia as being a key factor in maximizing MPS. This trial was registered at clinicaltrials.gov as NCT01735539.
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