Central Adiposity and Protein Intake Are Associated with Arterial Stiffness in Overweight Children

Being overweight is associated with vascular abnormalities, which are important in the development of atherosclerosis. However, little is known about dietary and lifestyle determinants of vascular function in overweight children. In adults, dietary protein and milk intake are associated with reduced blood pressure and reduced risk of metabolic syndrome. This study examined the associations between dietary protein, milk intake, physical activity, and adiposity on arterial stiffness in overweight children. In a cross-sectional study, overweight children with habitual milk intakes <= 250 mL/d were examined by DXA scans, pedometer counts, anthropometry, and metabolic variables. Dietary intake was registered for 4 d. The outcomes were arterial stiffness measured by pulse wave velocity (PWV) (n = 182) and augmentation index (Aix) (n = 183). The PWV (mean +/- SD) was 4.78 +/- 0.72 m/s and the Aix was -0.77 +/- 9.44%. In multivariate models, the android fat: gynoid fat and android fat:body fat ratios were positively associated with PWV (beta = 1.49 and beta = 10.3, both P < 0.05) and Aix (beta = 28.3, P < 0.01 and beta = 153, P < 0.05), whereas the gynoid fat:body fat ratio was negatively associated with the Aix (beta = -134; P < 0.001). Protein intake (percentage energy) was positively associated with PWV (beta = 0.05; P < 0.01). Milk intake (Lid) tended to be negatively associated with PWV (beta = -0.64; P = 0.05). Pedometer counts were negatively associated with the Aix; however, the association became nonsignificant after controlling for HOMA, which was positively associated with the Aix (beta = 0.95; P < 0.01). In conclusion, central adiposity and protein intake are associated with increased arterial stiffness measured as PWV in overweight children independent of blood pressure and heart rate. The effect of protein intake may be caused by meat, because the milk intake was low. J. Nutr. 142: 878-885, 2012.