Review
Plant Sciences
Yao Zheng, Cecile Cabassa-Hourton, Severine Planchais, Sandrine Lebreton, Arnould Savoure
Summary: Proline, an amino acid known for its role in proteins and osmotic regulation, has been found to have additional functions such as regulating redox balance and energy status. The well-described proline cycle in animals and plants is responsible for proline accumulation and redox equivalent transfer. This cycle not only affects proline levels, but also facilitates the transfer of reducing power between cellular compartments.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemical Research Methods
Walaa Abdallah, Xiao Hong, Scott Banta, Ian Wheeldon
Summary: Natural cascades often use spatial organization to introduce beneficial substrate channeling mechanisms, which has been widely adopted in engineered multienzyme cascades. Utilizing new molecular scaffolds now allows testing the effects of spatial organization on cascade kinetics, but these scaffolds can also change the microenvironment experienced by the assembled enzymes, complicating kinetic analysis.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Chaoyi Zhu, Xia You, Tao Wu, Wen Li, Hefeng Chen, Yaping Cha, Min Zhuo, Bo Chen, Shuang Li
Summary: This study describes the efficient biotransformation of mannitol into the high value-added sesquiterpenoid valencene using an evolved strain of Saccharomyces cerevisiae. The evolved strain exhibited superior performance in mannitol assimilation and showed significantly higher valencene titer and carbon conversion efficiency compared to glucose. Further engineering strategies increased the valencene titer, and the maximum titer obtained in a bioreactor was 5.61 g L-1. This research presents a novel strategy for terpenoid production in S. cerevisiae and expands the application of renewable biomass marine macroalgae.
Article
Biochemistry & Molecular Biology
Martina Chatzigianni, Dimitrios Savvas, Evgenia-Anna Papadopoulou, Konstantinos A. Aliferis, Georgia Ntatsi
Summary: This study aimed to investigate the impact of salinity and nitrogen supply on the primary metabolism of two ecotypes of Stamnagathi. The results showed that the montane ecotype had fluctuations in TCA cycle intermediates, while the seaside ecotype remained unaffected. Increase in proline levels and variable responses of other osmoprotectant metabolites were observed under low nitrogen supply and high salt stress conditions. The carbohydrate content of the plants was significantly affected by the treatments. The study also suggests that the seaside ecotype may have developed unique adaptation mechanisms, making it a promising candidate for breeding stress-tolerant varieties of C. spinosum.
Article
Environmental Sciences
Gholamreza Sayyadi, Yosoof Niknezhad, Hormoz Fallah
Summary: This study investigated the effects of nitric oxide (NO) on rice plants under lead (Pb) toxicity. The results showed that NO improved chlorophyll and proline metabolism, leading to enhanced growth and photosynthetic performance of rice plants under Pb stress. Additionally, NO reduced the accumulation of oxidative stress markers and promoted the immobilization of Pb in the roots. These findings suggest that NO could be a promising tool for enhancing the tolerance of rice plants to Pb toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Cell Biology
Hannah N. Bell, Amanda K. Huber, Rashi Singhal, Navyateja Korimerla, Ryan J. Rebernick, Roshan Kumar, Marwa O. El-derany, Peter Sajjakulnukit, Nupur K. Das, Samuel A. Kerk, Sumeet Solanki, Jadyn G. James, Donghwan Kim, Li Zhang, Brandon Chen, Rohit Mehra, Timothy L. Frankel, Balazs Gyorffy, Eric R. Fearon, Marina Pasca di Magliano, Frank J. Gonzalez, Ruma Banerjee, Daniel R. Wahl, Costas A. Lyssiotis, Michael Green, Yatrik M. Shah
Summary: There is a lack of effective therapies for advanced colorectal cancer (CRC) patients. In the CRC tumor microenvironment, there is an accumulation of metabolic waste products, such as ammonia, due to altered metabolism and proximity to the microbiota. The role of metabolite waste in tumor development, progression, and treatment resistance is not well understood. This study demonstrates that high ammonia levels induce T cell metabolic reprogramming, leading to T cell exhaustion and decreased proliferation. Enhancing ammonia clearance can reactivate T cells and enhance the efficacy of immunotherapies.
Article
Plant Sciences
Damian Balfagon, Jose L. Rambla, Antonio Granell, Vicent Arbona, Aurelio Gomez-Cadenas
Summary: The Mediterranean basin and other citrus-growing areas are highly vulnerable to the impacts of global warming, leading to water scarcity and high temperatures that affect citrus plant growth and yield. Grafting, specifically using Carrizo as the rootstock, has been shown to improve the metabolic and hormonal responses of Cleopatra scions to drought and heat stress, resulting in increased plant tolerance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Cell Biology
Marcin Naliwajski, Maria Sklodowska
Summary: The study found that acclimated cucumber plants showed higher antioxidant enzyme activity and alpha-tocopherol levels in response to salt stress compared to non-acclimated plants.
Article
Biochemistry & Molecular Biology
Adam Kazberuk, Magda Chalecka, Jerzy Palka, Katarzyna Bielawska, Arkadiusz Surazynski
Summary: This study found that non-steroidal anti-inflammatory drugs (NSAIDs) induce apoptosis in breast cancer cells through the activation of peroxisome proliferator-activated receptor-gamma (PPAR gamma) and the generation of reactive oxygen species (ROS) via proline dehydrogenase/proline oxidase (PRODH/POX). The effect of NSAIDs on cell apoptosis is independent of cyclooxygenase-2 (COX-2) inhibition. Additionally, the study showed that the role of PRODH/POX in NSAID-induced apoptosis is more pronounced in breast cancer cells with PRODH/POX knockout. The findings suggest a potential therapeutic strategy for cancer treatment using NSAIDs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Vanda Maria de Aquino Figueiredo, Silvana de Paula Quintao Scalon, Cleberton Correia Santos, Jessica Aline Linne, Juliana Milene Silverio, Wallas Matos Cerqueira, Joao Lucas da Costa Santos de Almeida
Summary: This study evaluated the effects of silicon and salicylic acid application on the growth and metabolism of Talisia esculenta Radlk. seedlings under water deficit conditions. The results showed that silicon application attenuated the harmful effects of water deficit on gas exchange and initial fluorescence, while salicylic acid application contributed to maintaining the relative water content of leaves.
Article
Plant Sciences
Mohamed Abdel-Mawgoud, Nahla Alsayd Bouqellah, Shereen Magdy Korany, Ahmed Mohamed Reyad, Abdelrahim H. A. Hassan, Emad A. Alsherif, Hamada Abdelgawad
Summary: This study demonstrated that the utilization of AMF can alleviate the harmful effects of thallium on the growth and metabolism of soybean plants. Thallium stress inhibited the growth and photosynthesis of soybean plants, but the application of AMF mitigated the toxicity. Additionally, AMF increased the levels of osmolytes and polyamines, as well as the activity of related enzymes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
S. K. Singh, T. Husain, M. Suhel, S. M. Prasad, V. P. Singh
Summary: This study investigates the alleviating effect of H2S on hexavalent chromium stress in two cereal crops (wheat and rice). The results show that supplementation with H2S can enhance the tolerance of crops to hexavalent chromium stress.
Article
Agronomy
Sushree Suparna Mahapatra, Dhaneswar Swain, Surjya Kanta Beura, Gyana Ranjan Rout
Summary: This study evaluated the resistance and susceptibility of mung bean genotypes against Cercospora canescens through field and greenhouse experiments. The results showed that there was a significant increase in enzymatic activities, total phenol, and proline contents in resistant and susceptible genotypes after inoculation, while the total chlorophyll content decreased. Higher enzyme activities were observed in the resistant genotypes compared to the susceptible genotypes.
Article
Plant Sciences
Lais L. Lima, Gabriella Frosi, Rafaela Lopes, Mauro Guida Santos
Summary: The study showed that Cenostigma pyramidale utilizes Na+ recirculation mechanisms to endure salt stress, with gas exchange and carbon metabolism being affected differently depending on the time in the day when the stress began. Morning-stressed plants exhibited more damage in terms of synthesized proline, H2O2, and malondialdehyde compared to night-stressed plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Veronica Castaneda, Esther M. Gonzalez
Summary: The study highlights the different response mechanisms of plants to drought stress and salinity stress. Plants enhance the protection of salt-stressed shoots and non-irrigated roots through regulating proline and carbon metabolism differently under these two pressure conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Bliss Kaneshiro, Alison Edelman, Chandravanu Dash, Jui Pandhare, Faapisa M. Soli, Jeffrey T. Jensen
Article
Microbiology
Chelsie Swepson, Alok Ranjan, Muthukumar Balasubramaniam, Jui Pandhare, Chandravanu Dash
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Muthukumar Balasubramaniam, Benem Davids, Amma B. Addai, Jui Pandhare, Chandravanu Dash
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Review
Virology
Muthukumar Balasubramaniam, Jui Pandhare, Chandravanu Dash
Article
Multidisciplinary Sciences
Sabyasachi Dash, Muthukumar Balasubramaniam, Chandravanu Dash, Jui Pandhare
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Virology
Muthukumar Balasubramaniam, Jing Zhou, Amma Addai, Phillip Martinez, Jui Pandhare, Christopher Aiken, Chandravanu Dash
JOURNAL OF VIROLOGY
(2019)
Article
Multidisciplinary Sciences
Binah Baht Ysrayl, Muthukumar Balasubramaniam, Ife Albert, Fernando Villalta, Jui Pandhare, Chandravanu Dash
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Hussein Kaddour, Yuan Lyu, Jennifer L. Welch, Victor Paromov, Sammed N. Mandape, Shruti S. Sakhare, Jui Pandhare, Jack T. Stapleton, Siddharth Pratap, Chandravanu Dash, Chioma M. Okeoma
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Multidisciplinary Sciences
Sabyasachi Dash, Muthukumar Balasubramaniam, Freddyson J. Martinez-Rivera, Arthur Godino, Emily G. Peck, Srinivas Patnaik, Mrutyunjay Suar, Erin S. Calipari, Eric J. Nestler, Fernando Villalta, Chandravanu Dash, Jui Pandhare
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Bobby Jones, Muthukumar Balasubramaniam, Joseph J. Lebowitz, Anne Taylor, Fernando Villalta, Habibeh Khoshbouei, Carrie Grueter, Brad Grueter, Chandravanu Dash, Jui Pandhare
Summary: The study reveals the essential role of proline biosynthesis in maintaining METH-induced glutamate homeostasis. METH exposure results in a significant upregulation of pyrroline-5-carboxylate synthase (P5CS), key enzyme for proline synthesis. Depletion of P5CS leads to an increase in glutamate levels upon METH exposure, emphasizing the protective mechanism to maintain glutamate homeostasis.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Ariana N. Renrick, Menaka C. Thounaojam, Maria Teresa P. de Aquino, Evan Chaudhuri, Jui Pandhare, Chandravanu Dash, Anil Shanker
Summary: The study demonstrates that bortezomib can enhance the function of anti-tumor CD8(+) T cells by inducing the downregulation of negative regulatory proteins SOCS1 and SHIP1 through the miR-155 pathway, thus overcoming T cell exhaustion in the tumor microenvironment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sabyasachi Dash, Chandravanu Dash, Jui Pandhare
Summary: Cocaine induces persistent cellular changes in brain reward regions by altering neuronal gene expression via chromatin remodeling. The energy-dependent PARP-1 catalyzed PARylation of p53 by cocaine results in nuclear accumulation of p53 and induction of proline oxidase, a gene involved in cellular metabolism. These findings demonstrate a novel metabolic pathway in response to cocaine treatment.
Article
Virology
Benem-Orom Davids, Muthukumar Balasubramaniam, Nicklas Sapp, Prem Prakash, Shalonda Ingram, Min Li, Robert Craigie, Thomas Hollis, Jui Pandhare, Chandravanu Dash
Summary: HIV-1 infection is associated with upregulation, perinuclear accumulation, and nuclear localization of TREX1. TREX1 plays a role in promoting HIV-1 integration by enhancing PIC activity and preferentially degrading viral DNAs that are incompatible with chromosomal insertion.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sabyasachi Dash, Chandravanu Dash, Jui Pandhare
Summary: The COVID-19 pandemic has caused devastation to global healthcare and economies. Targeting key host factors such as PARP-1 is proposed as a novel strategy to counter the virus. PARP-1 regulation by non-coding RNAs plays a critical role in cell survival and other cellular processes.
Article
Neurosciences
Sabyasachi Dash, Muthukumar Balasubramaniam, Tanu Rana, Arthur Godino, Emily G. Peck, Jeffery Shawn Goodwin, Fernando Villalta, Erin S. Calipari, Eric J. Nestler, Chandravanu Dash, Jui Pandhare