Article
Food Science & Technology
Runqi Fu, Hengzhi Zhang, Daiwen Chen, Gang Tian, Ping Zheng, Jun He, Jie Yu, Xiangbing Mao, Zhiqing Huang, Junning Pu, Wenwu Yang, Bing Yu
Summary: This study investigated the effects of dietary betaine supplementation on the growth performance, meat quality, and muscle lipid metabolism of growing-finishing pigs. The results showed that betaine supplementation significantly increased average daily gain and tended to improve average daily feed intake and feed intake to gain ratio. Furthermore, betaine supplementation had effects on meat quality, intramuscular fat content, and lipid metabolism in pigs.
Article
Chemistry, Applied
Yue Sun, Qi Wang, Xian Wu, Fang Li, Mingyue Song, Minqi Wang, Xiaokun Cai, Zhengze Li, Zili Gao, Jinkai Zheng, Ce Qi, Jin Sun, Hang Xiao
Summary: The study revealed that pterostilbene underwent demethylation, hydroxylation, and conjugation in the mouse gastrointestinal tract, with microbiota playing an important role in deconjugation and demethylation. The metabolites of pterostilbene may have significant implications for its bioactivity in mice.
Article
Agriculture, Dairy & Animal Science
Ivan Bahelka, Roman Stupka, Jaroslav Citek, Michal Sprysl, Ondrej Bucko, Pavel Flak
Summary: The European pig industry needs to address the concerns about animal welfare, especially regarding the surgical castration of male piglets. Several EU countries are trying to stop this practice, and the European Commission (EC) supports these efforts. One alternative to surgical castration is the fattening of entire male pigs with the use of additives, such as hydrolysable tannins. However, higher doses of tannins can negatively affect the juiciness and tenderness of pork from entire males, especially in men's evaluation.
Article
Microbiology
Zhongwei Xie, Junhua Du, Mailin Gan, Chengpeng Zhou, Menglin Li, Chengming Liu, Meng Wang, Lei Chen, Ye Zhao, Yan Wang, Yanzhi Jiang, Wenqiang Cheng, Kangping Zhu, Yi Luo, Li Zhu, Linyuan Shen
Summary: Short-term supplementation of choline significantly increases body weight gain and carcass weight in finishing pigs, potentially through alterations in gut microbiota and liver metabolism. This provides new insights into the potential of choline-mediated gut microbiota/metabolites in improving growth performance, carcass characteristics, and health.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Mervat A. Abdel-Latif, Ahmed R. Elbestawy, Ali H. El-Far, Ahmed E. Noreldin, Mohamed Emam, Roua S. Baty, Ghadeer M. Albadrani, Mohamed M. Abdel-Daim, Hatem S. Abd El-Hamid
Summary: The experiment demonstrated that quercetin supplementation can improve growth performance and gut environment in broiler chickens, as well as increase the amount of beneficial bacteria in the intestines.
Article
Food Science & Technology
Hanna Dai, Xiaoling Chen, Jun He, Ping Zheng, Yuheng Luo, Bing Yu, Daiwen Chen, Zhiqing Huang
Summary: This study investigated the effects of dietary L-theanine supplementation on finishing pigs' antioxidant capacity and lipid metabolism. The results showed that L-theanine supplementation increased the activity of antioxidant enzymes and reduced lipid levels in finishing pigs. Additionally, L-theanine activated a signaling pathway related to lipid metabolism and decreased the expression of genes related to adipogenesis. These findings suggest that L-theanine supplementation enhances antioxidant capacity and improves lipid metabolism in finishing pigs.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemistry & Molecular Biology
A. Risely, Phillip G. Byrne, Bethany J. Hoye, Aimee J. Silla
Summary: The amphibian skin microbiome is affected by carotenoid supplementation, which has subtle but widespread effects on the skin microbiome, even 4.5 years post supplementation. Carotenoid supplementation increases abundance of lactic acid bacteria, which are linked to protection against the amphibian fungal pathogen.
Article
Biochemistry & Molecular Biology
Zhongyang Guo, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jie Yu, Hui Yan, Ping Zheng, Yuheng Luo
Summary: This study utilized pigs as experimental subjects to investigate the effects of dietary dihydromyricetin (DHM) supplementation on antioxidant capacity and lipid metabolism. Results showed that DHM supplementation improved the antioxidant capacity and lipid metabolism in finishing pigs.
Article
Biotechnology & Applied Microbiology
Dongyan Zhang, Hui Liu, Sixin Wang, Wei Zhang, Shuaiqin Wang, Yamin Wang, Haifeng Ji
Summary: Supplementation with Lactobacillus showed gender-specific effects on fecal microbiota diversity and composition in pigs, with more pronounced effects on male pigs and significant impacts on metabolites in female pigs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Long, Yonghui Liu, Xihong Zhou, Liuqin He
Summary: The study showed that serine supplementation had no effect on selenium content in pigs, but 0.5% serine supplementation significantly increased selenium content in the liver. Supplementation with serine at different levels significantly increased the gene expression of selenoproteins in the skeletal muscle and liver of pigs. Pigs supplemented with 0.5% serine had the highest selenoprotein P concentration and enzymatic activity, while those with 0.25% serine had the highest enzymatic activity in the liver.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Nutrition & Dietetics
Miroslawa Cieslicka, Blazej Stankiewicz, Radoslaw Muszkieta, Malgorzata Tafil-Klawe, Jacek Klawe, Anna Skarpanska-Stejnborn, Joanna Ostapiuk-Karolczuk
Summary: This study investigated the effects of bovine colostrum supplementation on immune system functioning, iron management, and anabolic/catabolic balance in young football players. The results showed that supplementation significantly increased immunoglobulin G concentration and decreased inflammatory markers. However, it had no significant effect on athletes' performance, iron management, and hormonal response. Bovine colostrum could be a relatively mild and safe intervention for reducing inflammation induced by intense physical exercise.
Article
Fisheries
Jun Luo, Eric Amenyogbe, Wei-Jie Fu, Jian-Sheng Huang, Gang Chen
Summary: In this study, RNA sequencing of grouper liver revealed that quercetin and sodium quercetin-5'-sulfonates regulate gene expression in the liver, with down-regulation of genes involved in steroid biosynthesis pathway and up-regulation of genes related to polyamine synthesis. Glutaminasel (GLS1), the rate-limiting enzyme responsible for glutamine catabolism, was down-regulated by quercetin and sodium quercetin-5'-sulfonates exposure, suggesting its involvement in preventing fatty liver.
Article
Neurosciences
Hayarelis Moreno, Isabel de Brugada
Summary: Previous studies have shown that supplementing choline during the embryonic period can improve memory tasks in adulthood. This study aimed to investigate the effect of prenatal dietary choline supplementation on long-term memory development in rats. The results suggest that prenatal choline supplementation accelerates the development of long-term memory in rats.
NUTRITIONAL NEUROSCIENCE
(2021)
Article
Veterinary Sciences
Vasileios Kanoulas, Georgios A. Papadopoulos, Panagiotis Tassis, Georgia Koutouzidou, Georgios Arsenos, Paschalis Fortomaris
Summary: The study suggests that dietary supplementation of attapulgite can improve the growth and feed efficiency of pigs, especially when continuously supplemented after weaning.
VETERINARY SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Jose M. Rodriguez, M. Jose Diez, Matilde Sierra, Juan J. Garcia, Nelida Fernandez, Raquel Diez, Ana M. Sahagun
Summary: Flumequine concentrations in pig plasma, colon tissue and intestinal contents were not high enough to be effective against common intestinal pathogenic microorganisms in swine, including Brachyspira hyodysenteriae, suggesting that the dosage used may not be sufficient for treatment.
Article
Endocrinology & Metabolism
Bart Lagerwaard, Olga Pougovkina, Anna F. Bekebrede, Heleen te Brinke, Ronald J. A. Wanders, Arie G. Nieuwenhuizen, Jaap Keijer, Vincent C. J. de Boer
Summary: Post-translational protein modifications derived from metabolic intermediates, such as acyl-CoAs, have been shown to regulate mitochondrial function. Patients with a genetic defect in the propionyl-CoA carboxylase (PCC) gene clinically present symptoms related to mitochondrial disorders and are characterised by decreased mitochondrial respiration. Increased protein propionylation levels were observed in PCC deficient patients, leading to defective mitochondrial respiration, especially in the liver. Mimicking pathological protein propionylation levels in cultured cells resulted in a global increase in protein propionylation, which was correlated with decreased mitochondrial respiration in liver and fibroblasts, but not in muscle myotubes. This suggests that protein propionylation may play a role in the pathology of the disease, particularly in the liver.
JOURNAL OF INHERITED METABOLIC DISEASE
(2021)
Article
Multidisciplinary Sciences
Joelle J. E. Janssen, Bart Lagerwaard, Annelies Bunschoten, Huub F. J. Savelkoul, R. J. Joost van Neerven, Jaap Keijer, Vincent C. J. de Boer
Summary: Analyzing metabolism of PBMCs provides insights into various diseases, but new methods are needed to account for the dynamic nature of PBMCs. Our novel approach combining brightfield imaging with metabolic flux analysis improves accuracy and precision in XF assay data, reducing variability and increasing relevance in future studies.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Bart Lagerwaard, Marjanne D. van der Hoek, Joris Hoeks, Lotte Grevendonk, Arie G. Nieuwenhuizen, Jaap Keijer, Vincent C. J. de Boer
Summary: The study suggests that protein propionylation and propionyl-CoA may play important roles in muscle cell differentiation and aging through regulation of histone acylation.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Cecilia Loza, Supriya Verma, Siegfried Wolffram, Andreas Susenbeth, Ralf Blank, Friedhelm Taube, Ralf Loges, Mario Hasler, Christof Kluss, Carsten Stefan Malisch
Summary: Changes in agriculture towards simpler and more intensive systems have led to increased environmental problems, with ryegrass dominating temperate sown grasslands and limited use of forage legume species. Dicot forage plants rich in plant specialized metabolites, particularly tannins, have the potential to reduce methane emissions from ruminants. In vitro studies showed that dicot species reduced methane production, with chicory exhibiting the lowest methane emissions per digestible forage unit. Contrary to total gas production, methane production did not show linear changes with increasing proportions of legume or herb partner species.
Article
Nutrition & Dietetics
Inge van der Stelt, Wenbiao Shi, Melissa Bekkenkamp-Grovenstein, Ruben Zapata-Perez, Riekelt H. Houtkooper, Vincent C. J. de Boer, Maria A. Hegeman, Jaap Keijer
Summary: This study investigated how female mice respond to a diet that induces mild vitamin B-3 deficiency previously observed in male mice. The results showed that female mice did not exhibit vitamin B-3 deficiency under the same conditions where male mice did, highlighting the importance of studying both sexes in research.
EUROPEAN JOURNAL OF NUTRITION
(2022)
Article
Multidisciplinary Sciences
Xiang Zhang, Taolin Yuan, Jaap Keijer, Vincent C. J. de Boer
Summary: Seahorse XF analyzer is commonly used to measure mitochondrial OCR, but its complex data structure has not been adequately addressed in statistical analysis, impacting the robustness of statistical inference. OCRbayes, a Bayesian hierarchical modeling framework, has been developed to rigorously incorporate the complex data structure, showing potential for diagnosing patients with mitochondrial diseases.
Article
Geriatrics & Gerontology
Bart Lagerwaard, Arie G. Nieuwenhuizen, Annelies Bunschoten, Vincent C. J. Boer, Jaap Keijer
Summary: The study compared the transcriptome signatures of the vastus lateralis muscle in healthy older and young males with similar physical activity patterns, and found significant differences at the protein level, including downregulation of mitochondrial function and upregulation of inflammation processes. Despite similar physical activity levels, early affected physiological processes were observed in the elderly muscle.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Agriculture, Dairy & Animal Science
Edwin Westreicher-Kristen, Ralf Blank, Monika Paschke-Beese, Wiebke Kuehl, Siegfried Wolffram, Cornelia C. Metges, Andreas Susenbeth
Summary: The study aimed to evaluate the impact of replacing soybean meal crude protein with red clover silage in total mixed rations on ruminal degradation and intestinal digestibility of essential amino acids. The results showed that increasing levels of red clover silage in the diet led to higher ruminal degradation of EAA but reduced their intestinal digestibility, ultimately affecting the absorbable EAA in the intestine.
Article
Endocrinology & Metabolism
Joelle J. E. Janssen, Bart Lagerwaard, Mojtaba Porbahaie, Arie G. Nieuwenhuizen, Huub F. J. Savelkoul, R. J. Joost van Neerven, Jaap Keijer, Vincent C. J. de Boer
Summary: The mitochondrial metabolism in PBMCs from high-fit females is significantly higher than that in low-fit females, and this is not affected by PBMC composition or a recent exercise bout. Therefore, PBMC metabolism can be used as a marker to study the impact of lifestyle factors on human health.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Nutrition & Dietetics
Joelle J. E. Janssen, Bart Lagerwaard, Arie G. Nieuwenhuizen, Silvie Timmers, Vincent C. J. de Boer, Jaap Keijer
Summary: A single bout of exercise significantly increased EGR activity in both high-fit and low-fit females, but did not affect EGRAC, indicating that vitamin B2 status was not affected. The exercise response on EGRAC and EGR did not differ between high-fit and low-fit females.
Article
Multidisciplinary Sciences
A. F. Bekebrede, J. Keijer, W. J. J. Gerrits, V. C. J. de Boer
Summary: Intestinal epithelial cells are crucial for intestinal function and barrier maintenance, relying on energy production for proper function. A novel method was developed to accurately measure IEC metabolic function in order to gain better understanding of interventions on IEC function.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Evan E. E. Santo, Rasmus Ribel-Madsen, Peter J. J. Stroeken, Vincent C. J. de Boer, Ninna S. S. Hansen, Maaike Commandeur, Allan A. A. Vaag, Rogier Versteeg, Jihye Paik, Ellen M. M. Westerhout
Summary: This study identified a primate-specific transcriptional isoform named FOXO3A-Short (FOXO3A-S) in FOXO3A that is associated with human longevity. The study found that a specific SNP within the FOXO3A-S mRNA, rs9400239, influences the stability and functionality of the encoded protein. Carriers of the longevity T-allele have faster glucose clearance rates and longer lifespans.
Article
Biochemistry & Molecular Biology
Joelle J. E. Janssen, Bart Lagerwaard, Arie G. G. Nieuwenhuizen, Xavier Escote, Nuria Canela, Josep M. M. del Bas, Vincent C. J. de Boer, Jaap Keijer
Summary: This study examined the behavior of biomarkers and metabolic parameters in young healthy female adults with different aerobic fitness levels, as well as the impact of recent exercise on these markers. The results showed that overall biomarker and metabolic parameter profiles were similar between high-fit and low-fit females, but recent exercise had significant effects on certain individual biomarkers and metabolic parameters related to inflammation and lipid metabolism. This study provides valuable insights into the behavior of circulating biomarkers and metabolic parameters in healthy females and identifies functional categories that can be used to characterize human health physiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna F. Bekebrede, Thirza van Deuren, Walter J. J. Gerrits, Jaap Keijer, Vincent C. J. de Boer
Summary: Our study found that long-term exposure to butyrate did not affect mitochondrial bioenergetics in cultured cells, but decreased glycolytic function regardless of glucose availability. Furthermore, both high- and low-glucose-grown cells showed increased lipid accumulation after long-term butyrate exposure. Acute exposure to butyrate increased oxygen consumption rate (OCR) in cultured cells, with a simultaneous decrease in extracellular acidification rate (ECAR).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Bart Lagerwaard, Joelle J. E. Janssen, Iris Cuijpers, Jaap Keijer, Vincent C. J. de Boer, Arie G. Nieuwenhuizen
Summary: The study found that high aerobically fit females had faster m(V) over dotO(2) recovery in the gastrocnemius muscle compared to low aerobically fit females. Additionally, (V) over dotO(2)peak was significantly correlated with m(V) over dotO(2) recovery in both the gastrocnemius muscle and wrist flexors.
PHYSIOLOGICAL REPORTS
(2021)