期刊
JOURNAL OF NUCLEAR MEDICINE
卷 54, 期 12, 页码 2161-2167出版社
SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.112.115436
关键词
resveratrol; F-18-FDG; cancer; reactive oxygen species; HIF-1 alpha
资金
- Ministry of Health & Welfare, Republic of Korea [A110086]
- Korea Health Promotion Institute [A110086] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Resveratrol is gaining attention for its anticancer effects and is also recognized for its antioxidant properties and influence on glucose metabolism. Augmented reactive oxygen species (ROS) and high glycolytic flux are common characteristics of malignant cells. We thus evaluated the effect of resveratrol on cancer cell glucose metabolism and investigated the role of ROS in the response. Methods: Cancer cells were measured for cell content and F-18-FDG uptake. Assays were performed for lactate production; hexokinase activity and intracellular ROS; and immunoblotting for hypoxia-inducible factor-1 alpha (HIF-1 alpha), Akt, mammalian target of rapamycin, and glucose transporter type 1 (Glut-1). Animal studies were performed with small-animal PET imaging of Lewis lung carcinoma tumor bearing mice. Results: Resveratrol mildly decreased cell content and more pronouncedly suppressed F-18-FDG uptake in Lewis lung carcinoma, HT-29 colon, and T47D breast cancer cells. Hence, F-18-FDG uptake normalized to cell content was reduced to less than half of controls by 24-h exposure to resveratrol. This reduction was attributed to reduced glycolytic flux and Glut-1 expression. Resveratrol also decreased intracellular ROS in patterns that closely paralleled F-18-FDG uptake. Scavenging of ROS with N-acetyl cysteine, but not inhibition of nicotinamide adenine dinucleotide phosphate oxidase, was sufficient to suppress F-18-FDG uptake. Conversely, ROS inducers effectively reversed the metabolic response of resveratrol. HIF-1 alpha protein was markedly reduced by resveratrol, and inhibiting HIF-1 alpha expression with cycloheximide or specific small interfering RNAs suppressed F-18-FDG uptake. The proteosomal inhibitor MG132 partly restored HIF-1 alpha level and F-18-FDG uptake in resveratrol-treated cells. Resveratrol also inhibited Akt activation; in addition, inhibitors and small interfering RNAs against phosphoinositide 3-kinase decreased F-18-FDG uptake. Finally, small-animal PET results showed resveratrol treatment to suppress tumor F-18-FDG uptake in vivo. Conclusion: Resveratrol suppresses cancer cell F-18-FDG uptake and glycolytic metabolism in a manner that depends on the capacity of resveratrol to inhibit intracellular ROS, which downregulates HIF-1 alpha accumulation.
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