期刊
JOURNAL OF NUCLEAR MEDICINE
卷 51, 期 1, 页码 112-120出版社
SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.109.067074
关键词
positron emission tomography; brain imaging neuroimaging
资金
- Cooperative Research and Development Agreement with Eli Lilly
- Intramural Program of NIMH [Z01-MH-002852-04, Z01-MH002793-06]
- Academy of Finland
- Finnish Cultural Foundation
- Finnish Foundation for Alcohol Studies
- Finnish Medical Foundation
- Instrumentarium Foundation
- Jalmari and Rauha Ahokas Foundation
- Paulo Foundation
- Research Foundation of Orion Corporation
- Yrjo Jahnsson Foundation
- NATIONAL INSTITUTE OF MENTAL HEALTH [ZIAMH002852, ZIAMH002795, ZIAMH002793] Funding Source: NIH RePORTER
We recently demonstrated that C-11-MePPEP, a PET ligand for CB1 receptors, has such high uptake in the human brain that it can be imaged for 210 min and that receptor density can be quantified as distribution volume (V-T) using the gold standard of compartmental modeling. However, C-11-MePPEP had relatively poor retest and intersubject variabilities, which were likely caused by errors in the measurements of radioligand in plasma at low concentrations by 120 min. We sought to find an analog of C-11-MePPEP that would provide more accurate plasma measurements. We evaluated several promising analogs in the monkey brain and chose the F-18-di-deutero fluoromethoxy analog (F-18-FMPEP-d(2)) to evaluate further in the human brain. Methods: C-11-FMePPEP, F-18-FEPEP, F-18-FMPEP, and F-18-FMPEP-d(2) were studied in 5 monkeys with 10 PET scans. We calculated VT using compartmental modeling with serial measurements of unchanged parent radioligand in arterial plasma and radioactivity in the brain. Nonspecific binding was determined by administering a receptor-saturating dose of rimonabant, an inverse agonist at the CB1 receptor. Nine healthy human subjects participated in 17 PET scans using F-18-FMPEP-d(2), with 8 subjects having 2 PET scans to assess retest variability. To identify sources of error, we compared intersubject and retest variability of brain uptake, arterial plasma measurements, and VT. Results: F-18-FMPEP-d(2) had high uptake in the monkey brain, with greater than 80% specific binding, and yielded less radioactivity uptake in bone than did F-18-FMPEP. High brain uptake with F-18-FMPEP-d(2) was also observed in humans, in whom VT was well identified within approximately 60 min. Retest variability of plasma measurements was good (16%); consequently, VT had a good retest variability (14%), intersubject variability (26%), and intraclass correlation coefficient (0.89). VT increased after 120 min, suggesting an accumulation of radiometabolites in the brain. Radioactivity accumulated in the skull throughout the entire scan but was thought to be an insignificant source of data contamination. Conclusion: Studies in monkeys facilitated our development and selection of F-18-FMPEP-d(2), compared with F-18-FMPEP, as a radioligand demonstrating high brain uptake, high percentage of specific binding, and reduced uptake in bone. Retest analysis in human subjects showed that F-18-FMPEP-d(2) has greater precision and accuracy than C-11-MePPEP, allowing smaller sample sizes to detect a significant difference between groups.
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