Article
Chemistry, Medicinal
Masahiro Hashimoto, Takayuki Miki, Iou Ven Chang, Hiroshi Tsutsumi, Hisakazu Mihara
Summary: This study demonstrated the selection of biosensors that selectively bind to Gal-3 from an NBD-modified peptide phage library. The fluorescence intensity of these biosensors increased with Gal-3 concentration, with a visible fluorescent response.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Sarah Barakat, Melike Berksoz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman
Summary: Camelidae derived single-domain antibodies, known as nanobodies, are small antibody fragments with full antigen-binding capacity. They have gained popularity due to their small size, high specificity, strong affinity, excellent stability, and modularity. Nanobodies are now widely used as molecular probes in the field of molecular imaging, allowing the detection and tracking of various targets. This review provides an overview of their applications and discusses current techniques for their generation and delivery.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Analytical
Yaxue Dai, Yongjia Tang, Wenjing Huang, Yue Zhao, Xin Gao, Yueqing Gu
Summary: This study designed novel EpCAM-targeting peptides YQ-S for precise detection of breast cancer. YQ-S2 showed greater binding affinity and ideal biosafety profile, and was chosen to represent YQ-S. By linking with a near-infrared fluorescent dye, the EpCAM-targeting probe YQ-S2-MPA was developed for real-time imaging and fluorescence-guided resection of breast cancer tumors. In vivo experiments showed higher tumor uptake of YQ-S2-MPA compared to SNF-MPA, and successful detection of metastatic lesions. YQ-S2-MPA also performed excellently in surgical navigation studies in preclinical models.
Article
Biophysics
Rafael da Fonseca Alves, Isabella Castro Martins, Diego Leoni Franco, Adriane das Gracas Silva, Paula de Souza Santos, Luiz Ricardo Goulart, Yara Cristina de Paiva Maia, Alinne Tatiane Faria Silva, Thaise Goncalves Araujo, Maria Del Pilar Taboada Sotomayor
Summary: In this study, a new electrochemical biosensor was developed using peptides selected by Phage Display as bio-recognition phase for Breast Cancer (BC) characterization. The peptides were validated and found to have stronger reactivity to a specific type of BC. A new matrix for immobilizing biomolecules was generated through electrochemical modification of graphite electrodes. The biosensor was developed by immobilizing the peptides in the system for the detection of BC tumor markers in serological samples. The biosensor showed better performance compared to ELISA test.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Biochemistry & Molecular Biology
Hai Shin Pung, Gee Jun Tye, Chiuan Herng Leow, Woei Kean Ng, Ngit Shin Lai
Summary: Cancer is a major cause of death globally, with nearly 10 million deaths in 2020. The lack of effective screening methods and early detection contributes to the high mortality rate, preventing early intervention. Non-invasive and deep-tissue imaging is beneficial for cancer diagnosis, and targeting ligands can enhance sensitivity and specificity. Phage display technology identifies ligands with binding specificity for target receptors. Tumor-targeting peptides show promise in molecular imaging, and nanotechnology enables the design of more potent imaging probes for cancer diagnosis and targeting therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Medicine, General & Internal
Shruti Rajesh Patel, Joerg Herrmann, Robert A. Vierkant, Janet E. Olson, Fergus J. Couch, Antonious Hazim, Jeff A. Sloan, Charles L. Loprinzi, Kathryn J. Ruddy
Summary: This study compared the levels of cardiac biomarkers in breast cancer survivors who received anthracycline-based chemotherapy with those who did not. The findings suggest that NT-proBNP may be a biomarker for cardiovascular toxicity after anthracycline-based chemotherapy, while sST2 and galectin-3 do not differentiate between recipients and non-recipients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Jiayao Yan, Xiaoxiao Yu, Xiaotong Chen, Fangcen Liu, Fangjun Chen, Naiqing Ding, Lixia Yu, Fanyan Meng, Jie Shen, Jia Wei, Baorui Liu
Summary: The study identified a specific binding peptide F3 targeting GPC-3 through phage display, which showed promising applications in HCC tumor imaging and therapy. F3 peptide exhibited specific binding to HCC cells and enabled specific tumor detection through PET imaging, indicating its potential clinical value.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Daniel Xin Zhang, Xuan T. T. Dang, Luyen Tien Vu, Claudine Ming Hui Lim, Eric Yew Meng Yeo, Brenda Wan Shing Lam, Sai Mun Leong, Noorjehan Omar, Thomas Choudary Putti, Yu Chen Yeh, Victor Ma, Jia-Yuan Luo, William C. Cho, Gang Chen, Victor Kwan Min Lee, Andrew Grimson, Minh T. N. Le
Summary: Breast cancer cells release extracellular vesicles (EVs) that contain a large amount of biocargo, promoting intercellular communication and metastasis. It has been found that highly metastatic breast cancer cells have a higher enrichment of integrins, particularly alpha v and beta 1 subunits, in their EVs compared to poorly metastatic cells. Integrin alpha v has also been associated with disease progression in breast cancer patients. The export of integrin alpha v beta 1 into EVs is facilitated by members of the galectin family. Inhibition of the integrin alpha v beta 1 complex reduces the binding of EVs to fibronectin and decreases the metastatic potential of breast cancer cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Chemistry, Multidisciplinary
Hye Jin Lee, Metti K. Gari, David R. Inman, Zachary T. Rosenkrans, Brian M. Burkel, Aeli P. Olson, Jonathan W. Engle, Reinier Hernandez, Suzanne M. Ponik, Glen S. Kwon
Summary: The study demonstrates that PEGylated FUD peptide has improved tumor exposure in a murine breast cancer model compared to non-PEGylated FUD. PEG-FUD maintains nanomolar binding affinity for FN in vitro and stability for up to 48 hours, showing potential as a therapeutic and imaging agent for breast cancer.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Cell Biology
Nancy Nistico, Annamaria Aloisio, Antonio Lupia, Anna Maria Zimbo, Selena Mimmi, Domenico Maisano, Rossella Russo, Fabiola Marino, Mariangela Scalise, Emanuela Chiarella, Teresa Mancuso, Giuseppe Fiume, Daniela Omodei, Antonella Zannetti, Giuliana Salvatore, Ileana Quinto, Enrico Iaccino
Summary: In this study, we used phage display to select two highly specific peptide ligands for targeting the overexpressed EGFR in TNBC cells. Molecular docking predicted the peptides' binding affinities and sites on the extracellular domain of EGFR. Flow cytometry validated the binding of FITC-conjugated peptides to TNBC cells. Confocal microscopy confirmed the peptide binding specificity to EGFR-positive tumor tissues. These peptides could be used in conjunction with nanoparticles for tumor-targeted delivery of anticancer drugs.
Article
Pharmacology & Pharmacy
Dindyal Mandal, Sandeep Lohan, Muhammad Imran Sajid, Abdulelah Alhazza, Rakesh Kumar Tiwari, Keykavous Parang, Hamidreza Montazeri Aliabadi
Summary: In this study, several linear peptides containing tryptophan (W) and arginine (R) residues were designed and synthesized. These peptides were attached to lipophilic fatty acyl chains, cholesterol, or PEG. The newly developed peptides efficiently protected siRNA against early degradation and successfully delivered siRNA into cells, resulting in significant downregulation of target proteins.
Article
Biochemistry & Molecular Biology
Ana Claudia Pereira, Debora Ferreira, Catia Santos-Pereira, Tatiana F. Vieira, Sergio F. Sousa, Goreti Sales, Ligia R. Rodrigues
Summary: Diagnosing breast cancer remains a challenge due to its heterogeneity, which leads to delayed treatments and lower overall survival rates. Conventional detection techniques, while advancing in sophistication, still lack quick, convenient, and accurate results. Discovering novel biomarkers for breast cancer is crucial for early diagnosis.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Gastroenterology & Hepatology
Diyoly Ayona, Sandra Madariaga Zarza, Ludovic Landemarre, Benoit Roubinet, Philippe Decloquement, Didier Raoult, Pierre-Edouard Fournier, Benoit Desnues
Summary: Tropheryma whipplei is an actinobacterium that causes various infections in humans, including Whipple's disease. The bacterium's complex surface glycoproteins contain different sugars, and they interact with Galectin-1 and Galectin-3 to promote infection.
Review
Endocrinology & Metabolism
Deborah Simao Souza, Carla Macheroni, Gustavo Jose Silva Pereira, Carolina Meloni Vicente, Catarina Segreti Porto
Summary: Prostate cancer is the most common cancer among men worldwide, and hormone-related factors, such as androgens, estrogens, and their receptors, play a crucial role in its development and progression. Galectin-3, a family of proteins, is involved in various stages of tumor development and progression, but its expression regulation is still poorly understood. This review focuses on the interplay between Galectin-3 and estrogen receptor signaling pathways in prostate cancer cells and proposes a molecular mechanism involving ER, Galectin-3, and beta-catenin in the modulation of nuclear transcriptional events. Further understanding of the role of Galectin-3 and ER in prostate cancer could lead to the discovery of new drug targets to treat castration-resistant prostate cancer.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Oncology
Iwona Radziejewska
Summary: MUC1 mucin and galectin-3 with high affinity to T disaccharide are both overexpressed in human cancers, supporting cancer development and metastasis. Inhibiting their interactions may reduce tumor progression and metastasis. This review aims to summarize the relationship between MUC1 and galectin-3.