Article
Neurosciences
Hisham Sharif, Heather Alexander, Anika Azam, John H. Martin
Summary: The study found that dual epidural motor cortex intermittent theta burst stimulation (iTBS) and cathodal transcutaneous spinal direct stimulation (tsDCS) enhance the efficacy of rehabilitation in improving motor function after spinal cord injury (SCI). Stimulation+rehabilitation group showed increased corticospinal tract axon length caudal to the injury, reduced proprioceptive afferent terminal labeling in the intermediate zone, and a negative correlation between afferent fiber terminal labeling and motor recovery.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Neurosciences
Tokiharu Sato, Yuka Nakamura, Akinori Takeda, Masaki Ueno
Summary: The study found that after stroke, remaining contralesional motor and sensory CST axons crossed the midline and sprouted into the denervated side of the cervical spinal cord in a large cortical area. However, in smaller strokes, contralesional CST fibers did not sprout, while ipsilesional axons from spared motor areas grew on the denervated side. The research also showed that motor and sensory CST axons did not innervate the projecting areas mutually when either one was injured.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Neurosciences
Frances S. Hausmann, John M. Barrett, Megan E. Martin, Huiqing Zhan, Gordon M. G. Shepherd
Summary: This study used MAPseq to investigate the diversity of brainstem branching patterns of the pyramidal tract projections from the forelimb-related areas of the motor cortex. The results showed high yet systematic diversity in PT axons at the level of brainstem projection patterns. The projections from M1 and M2 were qualitatively similar, but with quantitative differences in subclasses and clusters.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Biwu Wu, Lei Yang, Caihua Xi, Haijun Yao, Long Chen, Fengqi Fan, Gang Wu, Zhouying Du, Jin Hu, Shukun Hu
Summary: This study reveals a role of Shh in enhancing neuroplasticity in the adult central nervous system, leading to improved outcomes of rehabilitative training.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Ryota Nakahama, Aika Saito, Sensho Nobe, Kazuya Togashi, Ikuo K. Suzuki, Akira Uematsu, Kazuo Emoto
Summary: This study aimed to enhance the transgene expression efficiency of AAV2-retro vectors by introducing YF mutations in the virus capsid. The results showed that this mutation significantly increased transgene expression levels and retrograde transduction in neurons. These findings are important for dissecting neural circuits and future clinical applications.
Article
Neurosciences
Weiping Pan, Xueyin Huang, Zikai Yu, Qiongqiong Ding, Liping Xia, Jianfeng Hua, Bokai Gu, Qisong Xiong, Hualin Yu, Junbo Wang, Zhenzhong Xu, Linghui Zeng, Ge Bai, Huaqing Liu
Summary: Diabetic neuropathic pain (DNP) is a common complication of diabetes where the aberrant sprouting of sensory axons into the epidermal area is linked to its pathogenesis. The axon guidance molecule, Netrin-3 (Ntn-3), was found to be expressed in sensory neurons and its downregulation was correlated with the severity of DNP in a mouse model. Genetic ablation of Ntn-3 increased axon sprouting and worsened DNP, while elevation of Ntn-3 levels inhibited axon sprouting and alleviated DNP. These findings indicate that Ntn-3 is an important regulator of DNP and a potential target for treatment.
NEUROSCIENCE BULLETIN
(2023)
Article
Neurosciences
Audra A. Kramer, Greta M. Olson, Advaita Chakraborty, Murray G. Blackmore
Summary: Forced expression of the transcription factor KLF6 enhanced axon sprouting in adult CST neurons after spinal injuries, but this effect required an injury stimulus and showed significant growth enhancement within four weeks. However, treatment with KLF6 did not lead to significant improvements in forelimb function, despite robust cross-midline sprouting by CST axons. Additional interventions or further optimization may be needed to translate the improvements in axon growth into functional gains.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Neurosciences
Mariajose Metcalfe, Oswald Steward
Summary: Spinal cord injuries cause permanent functional impairments, but the deletion of the PTEN gene can promote the recovery of motor function. Injections of AAV-rg/Cre can enhance forelimb motor recovery after SCI, but late-developing functional abnormalities occur with the experimental conditions used here.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Neurosciences
Mariajose Metcalfe, Kelly M. Yee, Juan Luo, Jacob H. Martin-Thompson, Sunil P. Gandhi, Oswald Steward
Summary: This paper explores the potential of using rAAV2-retro to deliver gene modifying cargoes to the cells of origin of multiple pathways affected by spinal cord injury (SCI). Results show that rAAV-retro exhibits effective and robust retrograde transport from pre-terminal axons back to neuronal bodies. It can simultaneously transduce large numbers of neurons of origin of multiple spinal pathways with single injections. The study demonstrates the potential applications of rAAV-retro for promoting axon regeneration after SCI.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Virology
Andrew D. Esteves, Orkide O. Koyuncu, Lynn W. Enquist
Summary: Neurons are highly polarized cells with axons that can reach centimeters in length. Communication between axons and distant cell bodies involves slow active transport of chemical messengers. The viral kinase US3 plays a critical role in promoting efficient retrograde transport of nucleocapsids in axons, leading to productive infection.
JOURNAL OF VIROLOGY
(2022)
Review
Cell Biology
Hajime Takase, Robert W. Regenhardt
Summary: Acute central nervous system injuries, particularly involving the motor tract, can lead to disability and have significant social and economic impacts. Understanding the pathophysiology of injury and subsequent motor tract reorganization is crucial for developing therapies to enhance recovery after such injuries, including targeted rehabilitation and novel pharmacotherapies. Experimental data suggest a promising future with potential advances in neurotechnologies and alignment of preclinical studies with human clinical trials.
NEURAL REGENERATION RESEARCH
(2021)
Review
Neurosciences
Chase A. Weinholtz, Michael J. Castle
Summary: Emerging techniques using axonal transport of adeno-associated virus (AAV) vectors can precisely target gene expression in specific neuron populations based on their axonal connectivity, allowing researchers to dissect neural circuits and understand the structure and function of the nervous system in mammals.
JOURNAL OF NEUROSCIENCE RESEARCH
(2021)
Article
Neurosciences
Maria de Fatima dos Santos Sampaio, Marcela Santana Bastos Boechat, Igor Augusto Gusman Cunha, Messias Gonzaga Pereira, Norberto Cysne Coimbra, Arthur Giraldi-Guimaraes
Summary: This study demonstrates that intravenous treatment with BMMCs promotes the reconnection of lesioned cortico-cortical and cortico-striatal pathways, increases axonal sprouting, and improves motor performance. In the acute phase of ischaemia, upregulation of Neurotrophin-3 in response to the lesion is observed, while long-term therapy with BMMCs leads to axonal sprouting, reconnection of damaged neuronal circuitry, and a significant enhancement in motor performance.
Article
Neurosciences
Masahiro Sawada, Kimika Yoshino-Saito, Taihei Ninomiya, Takao Oishi, Toshihide Yamashita, Hirotaka Onoe, Masahiko Takada, Yukio Nishimura, Tadashi Isa
Summary: This study investigates the morphologic changes in the corticospinal tract (CST) after injury, and reveals that multiple reorganizations of the corticospinal projections to spinal segments contribute to the recovery of hand function after spinal cord injury.
Article
Developmental Biology
Cody J. Drozd, Christopher C. Quinn
Summary: This study reveals the role of UNC-116/KIF5C and NEKL-3/NEK6/7 kinase in axon targeting in Caenorhabditis elegans. UNC-116 and UNC-16/JIP3 play important roles in axon termination, and NEKL-3 functions with RPM-1 ubiquitin ligase to promote axon termination.
Review
Neurosciences
Damien D. Pearse, Zoe A. Hughes
Review
Neurosciences
Thomas Moller, Frederique Bard, Anindya Bhattacharya, Knut Biber, Brian Campbell, Elena Dale, Claudia Eder, Li Gan, Gwenn A. Garden, Zoe A. Hughes, Damien D. Pearse, Roland G. W. Staal, Faten A. Sayed, Paul D. Wes, Hendrikus W. G. M. Boddeke
Article
Immunology
Mousumi Ghosh, Yong Xu, Damien D. Pearse
JOURNAL OF NEUROINFLAMMATION
(2016)
Article
Neurosciences
Johana Bastidas, Gagani Athauda, Gabriela De La Cruz, Wai-Man Chan, Roozbeh Golshani, Yerko Berrocal, Martha Henao, Anil Lalwani, Chikato Mannoji, Mazen Assi, P. Anthony Otero, Aisha Khan, Alexander E. Marcillo, Michael Norenberg, Allan D. Levi, Patrick M. Wood, James D. Guest, W. Dalton Dietrich, Mary Bartlett Bunge, Damien D. Pearse
Article
Biochemistry & Molecular Biology
Dominic M. Maggio, Amanpreet Singh, J. Bryan Iorgulescu, Drew H. Bleicher, Mousumi Ghosh, Michael M. Lopez, Luis M. Tuesta, Govinder Flora, W. Dalton Dietrich, Damien D. Pearse
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Review
Biochemistry & Molecular Biology
Eric P. Knott, Mazen Assi, Sudheendra N. R. Rao, Mousumi Ghosh, Damien D. Pearse
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Critical Care Medicine
Kim D. Anderson, James D. Guest, W. Dalton Dietrich, Mary Bartlett Bunge, Rosie Curiel, Marine Dididze, Barth A. Green, Aisha Khan, Damien D. Pearse, Efrat Saraf-Lavi, Eva Widerstrom-Noga, Patrick Wood, Allan D. Levi
JOURNAL OF NEUROTRAUMA
(2017)
Article
Biochemistry & Molecular Biology
Damien D. Pearse, Johana Bastidas, Sarah S. Izabel, Mousumi Ghosh
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Clinical Neurology
Joshua D. Burks, Katie L. Gant, James D. Guest, Aria G. Jamshidi, Efrem M. Cox, Kim D. Anderson, W. Dalton Dietrich, Mary Bartlett Bunge, Barth A. Green, Aisha Khan, Damien D. Pearse, Efrat Saraf-Lavi, Allan D. Levi
NEUROSURGICAL FOCUS
(2019)
Article
Orthopedics
Harvey Chim, Christopher J. Salgado, Nicole L. Miller, Erin M. Wolfe, Sudheendra Rao, Damien D. Pearse
JOURNAL OF HAND SURGERY-AMERICAN VOLUME
(2019)
Meeting Abstract
Surgery
Erin M. Wolfe, Sydney A. Mathis, Steven Ovadia, Taylor Wiener, Daisy Gonzalez, Prakash Mathew, Damien D. Pearse, Christopher J. Salgado, Zubin J. Panthaki
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2019)
Article
Neurosciences
Damien D. Pearse, Sudheendra N. R. Rao, Alejo A. Morales, Warren Wakarchuk, Urs Rutishauser, Abderrahman El-Maarouf, Mousumi Ghosh
Summary: This study compared two methods of inducing PolySia on Schwann cells and found that PSTNm was more effective in enhancing cell migration and wound closure. PolySia induction significantly improved neurorepair after spinal cord injury and had effects on both host cells and transplanted cells.
NEUROSCIENCE LETTERS
(2021)
Article
Neurosciences
Damien D. Pearse, Peter Anthony Otero, Ashley Diaz, Xiaoqi Pan, Mousumi Ghosh
Summary: TG2 is a multifunctional enzyme involved in the pathogenesis of EAE and MS. It is found to be induced in neurons following EAE, particularly around perivascular lesion sites, and co-localizes with proteins related to apoptosis and ERK1/2 signaling. Additionally, TG2 is also expressed in vascular cells and relates to the upregulation of VEGF. Its cell autonomous functions during disease progression remain to be elucidated.
Meeting Abstract
Cell & Tissue Engineering
D. D. Pearse, J. Bastidas, S. S. Izabel, Y. Xu, A. A. Morales, M. Ghosh
CELL TRANSPLANTATION
(2018)
Meeting Abstract
Cell & Tissue Engineering
M. Ghosh, Y. Xu, A. A. Morales, A. El Maarouf, U. Rutishauser, D. D. Pearse
CELL TRANSPLANTATION
(2018)