Review
Neurosciences
Frederic A. A. Vallejo, Anthony Diaz, Emily L. L. Errante, Taylor Smartz, Aisha Khan, Risset Silvera, Adriana E. E. Brooks, Yee-Shuan Lee, Stephen Shelby Burks, Allan D. D. Levi
Summary: This study systematically evaluated the therapeutic use of Schwann cells (SC) in the repair of peripheral nerve injuries. The results showed that SCs have clear potential in improving morphological and functional outcomes in animal studies of long gap peripheral nerve injuries. However, the optimal nerve conduit or graft for delivering and retaining SCs for nerve regeneration has yet to be determined.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Emily L. Errante, Anthony Diaz, Taylor Smartz, Aisha Khan, Risset Silvera, Adriana E. Brooks, Yee-Shuan Lee, S. Shelby Burks, Allan D. Levi
Summary: Peripheral nerve injury (PNI) is common in trauma patients, and severe PNI can be challenging to treat. The use of cell therapies, particularly Schwann cells, has shown effectiveness in PNI treatment. Our laboratory has demonstrated the effectiveness of Schwann cells in the treatment of severe PNI when used with axon guidance channels, but optimal cellular placement techniques are crucial.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Andres Fuentes-Flores, Cristian Geronimo-Olvera, Karina Girardi, David Necunir-Ibarra, Sandip Kumar Patel, Joanna Bons, Megan C. Wright, Daniel Geschwind, Ahmet Hoke, Jose A. Gomez-Sanchez, Birgit Schilling, Daniela L. Rebolledo, Judith Campisi, Felipe A. Court
Summary: Reparative Schwann cells transition into a senescent phenotype in aging and chronic denervation, leading to inhibition of axonal regeneration. Elimination of senescent cells improves nerve regeneration and functional recovery.
EMBO MOLECULAR MEDICINE
(2023)
Article
Biology
Tian-Kun Hui, Xin-Sheng Lai, Xia Dong, Hongyang Jing, Ziyang Liu, Erkang Fei, Wen-Bing Chen, Shunqi Wang, Dongyan Ren, Suqi Zou, Hai-Tao Wu, Bing-Xing Pan
Summary: Schwann cells play a crucial role in peripheral nerve regeneration, while the conditional knockout of Lrp4 in SCs contributes to enhanced nerve regeneration by promoting SC proliferation and myelin debris clearance. These results suggest a novel role for Lrp4 in peripheral nerve recovery and provide a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Jose A. Gomez-Sanchez, Nikiben Patel, Fernanda Martirena, Shaline Fazal, Clara Mutschler, Hugo Cabedo
Summary: The peripheral nervous system (PNS) has superior regenerative capacity compared to the central nervous system (CNS). This regenerative ability is attributed to the reprogramming of Schwann cells (SC) into repair Schwann cells, which support axonal growth, myelin degradation, neurotrophic factor secretion, and axonal growth guidance. Epigenetic regulations, particularly histone acetylation and deacetylation, play a crucial role in SC reprogramming and nerve regeneration. This review focuses on Zn2+-dependent histone deacetylases (HDACs) and their importance in repair SC biology and remyelination after PNS injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Carina Hromada, Jaana Hartmann, Johannes Oesterreicher, Anton Stoiber, Anna Daerr, Barbara Schaedl, Eleni Priglinger, Andreas H. Teuschl-Woller, Wolfgang Holnthoner, Johannes Heinzel, David Hercher
Summary: This study reveals increased lymphangiogenesis after peripheral nerve injury and repair. The findings suggest that Schwann cells may have an inhibitory effect on lymphatic vessel formation.
Review
Neurosciences
Qing Min, David B. Parkinson, Xin-Peng Dun
Summary: Schwann cells in the peripheral nervous system have the ability to promote repair of various tissues, including nerve gaps, skin wounds, digit tips, and tooth regeneration. They are highly motile, secrete signaling molecules, attract macrophages, support neuronal survival, promote axonal regrowth, and interact with other cell types in the regeneration process. The migration of Schwann cells is essential for successful tissue repair, especially in cases of peripheral nerve transection injuries where the cells form cords to guide axon regeneration. Understanding the molecular mechanisms of Schwann cell migration may lead to new therapeutic strategies for peripheral nerve repair.
Article
Engineering, Environmental
Ying Ma, Hao Wang, Qiqi Wang, Xiaodong Cao, Huichang Gao
Summary: This study developed a multi-channel nerve guidance conduit (NGC) with dual electrical activity of piezoelectricity and conductivity to enhance peripheral nerve regeneration. In vitro and in vivo experiments demonstrated that the electroactive NGC promoted cell proliferation, differentiation, axonal regeneration, and functional recovery of peripheral nerves, indicating its great potential in repairing peripheral nerve injuries.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Cell & Tissue Engineering
Liangfu Jiang, Thomas Mee, Xijie Zhou, Xiaofeng Jia
Summary: This review discusses the critical role of adipose-derived stem cells (ADSCs) in treating PNIs, including ADSCs differentiating into Schwann cells, secreting growth factors to promote nerve growth, promoting myelination growth, and treatment with scaffolds. It compares the roles of ADSCs and mesenchymal stem cells in regenerative treatment, and discusses administration routes, cell dosages, and cell fates. ADSCs secrete neurotrophic factors and exosomes, and have the potential to differentiate into Schwann cell-like cells that can promote nerve regeneration in the peripheral nervous system.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Neurosciences
Deepak Prasad Gupta, Anup Bhusal, Md Habibur Rahman, Jae-Hong Kim, Youngshik Choe, Jaemyung Jang, Hyun Jin Jung, Un-Kyung Kim, Jin-Sung Park, Lee-so Maeng, Kyoungho Suk, Gyun Jee Song
Summary: This study reveals the essential role of EBP50 in the repair function of Schwann cells and regeneration following nerve injury. EBP50 deficiency leads to deficits in Schwann cell functions and delays nerve repair and functional recovery. Additionally, EBP50 enhances regeneration by binding and activating ErbB2.
PROGRESS IN NEUROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Peng Yu, Guanhua Zhang, Bo Hou, Enpeng Song, Jiaming Wen, Yueyang Ba, Donglin Zhu, Gangwei Wang, Feng Qin
Summary: Complete myelination and formation of myelinated fibers are essential for functional nerve regeneration after peripheral nerve injury. Suboptimal myelin regeneration is common and can hinder ideal nerve regeneration. Therefore, closely monitoring and supporting myelin regeneration in patients with PNI is important for optimal outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Orthopedics
Guidong Shi, Dingyu Hao, Lei Zhang, Jia Qin, Guangyuan Tian, Boyuan Ma, Xianhu Zhou
Summary: Through analysis of normal and activated Schwann cells, this study identified key genes and proteins involved in endocytosis and exosomes which may control the molecular mechanism of peripheral nerve repair. The study suggests that targeting endocytosis-associated pathways in Schwann cells could be a novel therapeutic approach for nerve tissue engineering and regeneration.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Bing Xia, Xue Gao, Jiaqi Qian, Shengyou Li, Beibei Yu, Yiming Hao, Bin Wei, Teng Ma, Haining Wu, Shijie Yang, Yi Zheng, Xueli Gao, Lingli Guo, Jianbo Gao, Yujie Yang, Yongfeng Zhang, Yitao Wei, Borui Xue, Yan Jin, Zhuojing Luo, Jin Zhang, Jinghui Huang
Summary: A superparamagnetic nanocomposite scaffold is developed to control the production of extracellular vesicles (EVs) by applying a rotating magnetic field. This non-invasive and remotely time-scheduled method accelerates tissue regeneration, including that of peripheral nerves.
ADVANCED MATERIALS
(2023)
Review
Neurosciences
Qian Wang, Fang-Yu Chen, Zhuo-Min Ling, Wen-Feng Su, Ya-Yu Zhao, Gang Chen, Zhong-Ya Wei
Summary: Peripheral neuropathy is a common neurological issue with limited treatment options. Schwann cell transplantation plays a crucial role in resolving peripheral neuropathy, and stem cells have the potential to differentiate into Schwann-like cells. These studies provide insights into cell therapy and uncover prospective therapeutic strategies for peripheral neuropathy.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Urology & Nephrology
Luisa Muratori, Federica Fregnan, Giulia Ronchi, Kirsten Haastert-Talini, Jennifer Metzen, Riccardo Bertolo, Francesco Porpiglia, Stefano Geuna
Article
Cell & Tissue Engineering
Giulia Ronchi, Giovanna Gambarotta, Michela Morano, Federica Fregnan, Pierfrancesco Pugliese, Pierluigi Tos, Stefano Geuna, Kirsten Haastert-Talini
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Review
Cell Biology
Zhong Huang, Rebecca Powell, James B. Phillips, Kirsten Haastert-Talini
Article
Anatomy & Morphology
Anja Boeckers, Horst Claassen, Kirsten Haastert-Talini, Jurgen Westermann
Summary: In this viewpoint, representatives of the Teaching Commission of the Anatomical Society summarize their teaching experiences during the COVID-19 pandemic and recommend universities to prioritize face-to-face teaching while also using online teaching as necessary. The transition to remote learning during the pandemic revealed technical shortcomings and deficits in practical hands-on teaching.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2021)
Review
Neurosciences
Svenja Kankowski, Claudia Grothe, Kirsten Haastert-Talini
Summary: This review discusses neuropathic pain development and handling. The first part describes the pathways of neuropathic pain and different in vivo models. The second part presents the knowledge about cellular and molecular mechanisms that may lead to neuropathic pain. Established animal models can simulate the diversity of neuropathic pain observed in clinics.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Marwa El Soury, Benedetta Elena Fornasari, Giacomo Carta, Federica Zen, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerves are easily prone to injuries and require supportive strategies to stimulate nerve growth and functional recovery. The impact of diet on health and body function is crucial, with balanced nutrition being essential for the health of the central and peripheral nervous systems. Many dietary supplements have shown to play a significant role in peripheral nerve development and regeneration, emphasizing the importance of tailored dietary plans for patients with nerve injuries in promoting nerve regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhong Huang, Svenja Kankowski, Ella Ertekin, Mara Almog, Zvi Nevo, Shimon Rochkind, Kirsten Haastert-Talini
Summary: The study found that hydrogel luminal fillers can play a positive role in nerve regeneration, but functional recovery still falls short compared to autologous nerve grafts. In addition, M-HAL showed improvement in the performance of different types of hollow nerve guides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Oleksandr Gryshkov, Fedaa Al Halabi, Antonia Isabel Kuhn, Sara Leal-Marin, Lena Julie Freund, Maria Forthmann, Nils Meier, Sven-Alexander Barker, Kirsten Haastert-Talini, Birgit Glasmacher
Summary: PVDF and P(VDF-TrFE) are promising biomaterials for nerve regeneration, with piezoelectric properties stimulating cell growth. The study compared their structural and piezoelectric properties, using a dynamic impact test machine. PVDF showed increased beta-phase content post-electrospinning and similar zeta potential to P(VDF-TrFE), while also demonstrating guiding properties for nerve outgrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Luisa Muratori, Federica Fregnan, Monica Maurina, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerve injuries can lead to loss of motor and sensory function, and current methods for nerve regeneration have limited success. A potential approach for enhancing peripheral nerve regeneration is through improving diet and lifestyle, and consuming polyphenols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Neurosciences
Kirsten Haastert-Talini
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zhong Huang, Rebecca Powell, Svenja Kankowski, James B. Phillips, Kirsten Haastert-Talini
Summary: Researchers have successfully generated Schwann cells from human induced pluripotent stem cells by modifying existing protocols, resulting in sufficient cell numbers. They also found that specific coating and glucose levels are crucial for improving the differentiation process and increasing the yield of viable cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sonia Calabro, Svenja Kankowski, Matilde Cescon, Giovanna Gambarotta, Stefania Raimondo, Kirsten Haastert-Talini, Giulia Ronchi
Summary: It is widely recognized that the gut microbiota plays a crucial role in human health, and its disruption is associated with various diseases. Recent studies have expanded our understanding of the relationship between the gut microbiota and the peripheral nervous system (PNS), highlighting that the PNS can be affected by GM perturbations. This review provides a comprehensive summary of the current knowledge on the impact of the gut microbiota on the PNS, considering both its somatic and autonomic divisions in physiological, regenerative, and pathological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Nina Dietzmeyer, Zhong Huang, Tobias Schuening, Shimon Rochkind, Mara Almog, Zvi Nevo, Thorsten Lieke, Svenja Kankowski, Kirsten Haastert-Talini
CELL TRANSPLANTATION
(2020)
Article
Medicine, General & Internal
Alexander Kaltenborn, Nicco Krezdorn, Sebastian Hoffmann, Andre Gutcke, Kirsten Haastert-Talini, Peter M. Vogt, Axel Haverich, Bettina Wiegmann
MILITARY MEDICAL RESEARCH
(2020)
Review
Cell Biology
Nina Dietzmeyer, Maria Foerthmann, Claudia Grothe, Kirsten Haastert-Talini
NEURAL REGENERATION RESEARCH
(2020)
Article
Biochemical Research Methods
Aline Silva da Cruz, Maria Margarida Drehmer, Wagner Baetas-da-Cruz, Joao Carlos Machado
Summary: This study quantified microcirculation cerebral blood flow in a rat model of ischemic stroke using ultrasound biomicroscopy and ultrasound contrast agents. The results showed high sensitivity and specificity of this method, making it a valuable tool for preclinical studies.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Christina Dalla, Ivana Jaric, Pavlina Pavlidi, Georgia E. Hodes, Nikolaos Kokras, Anton Bespalov, Martien J. Kas, Thomas Steckler, Mohamed Kabbaj, Hanno Wuerbel, Jordan Marrocco, Jessica Tollkuhn, Rebecca Shansky, Debra Bangasser, Jill B. Becker, Margaret McCarthy, Chantelle Ferland-Beckham
Summary: Many funding agencies have emphasized the importance of considering sex as a biological variable in experimental design to improve the reproducibility and translational relevance of preclinical research. Omitting the female sex from experimental designs in neuroscience and pharmacology can result in biased or limited understanding of disease mechanisms. This article provides methodological considerations for incorporating sex as a biological variable in in vitro and in vivo experiments, including the influence of age and hormone levels, and proposes strategies to enhance methodological rigor and translational relevance in preclinical research.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Wenyu Gu, Dongxu Li, Jia-Hong Gao
Summary: We developed a precise and rapid method for positioning and labelling triaxial OPMs on a wearable magnetoencephalography (MEG) system, improving the efficiency of OPM positioning and labelling.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Kai Lin, Linhang Zhang, Jing Cai, Jiaqi Sun, Wenjie Cui, Guangda Liu
Summary: The article introduces an EEG feature map processing model for emotion recognition, which achieves significantly improved accuracy by fusing EEG information at different spatial scales and introducing a channel attention mechanism.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
John E. Parker, Asier Aristieta, Aryn H. Gittis, Jonathan E. Rubin
Summary: This work presents a toolbox that implements a methodology for automated classification of neural responses based on spike train recordings. The toolbox provides a user-friendly and efficient approach to detect various types of neuronal responses that may not be identified by traditional methods.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Yun Liang, Ke Bo, Sreenivasan Meyyappan, Mingzhou Ding
Summary: This study compared the performance of SVM and CNN on the same datasets and found that CNN achieved consistently higher classification accuracies. The classification accuracies of SVM and CNN were generally not correlated, and the heatmaps derived from them did not overlap significantly.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Antonino Visalli, Maria Montefinese, Giada Viviani, Livio Finos, Antonino Vallesi, Ettore Ambrosini
Summary: This study introduces an analytical strategy that allows the use of mixed-effects models (LMM) in mass univariate analyses of EEG data. The proposed method overcomes the computational costs and shows excellent performance properties, making it increasingly important in the field of neuroscience.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Xavier Cano-Ferrer, Alexandra Tran -Van -Minh, Ede Rancz
Summary: This study developed a novel rotation platform for studying neural processes and spatial navigation. The platform is modular, affordable, and easy to build, and can be driven by the experimenter or animal movement. The research demonstrated the utility of the platform, which combines the benefits of head fixation and intact vestibular activity.
JOURNAL OF NEUROSCIENCE METHODS
(2024)