期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 188, 期 1, 页码 127-140出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2010.01.030
关键词
Anchovy; Area centralis; Acute vision; Cell pattern; Convergence; Fish eye; Polarization vision
资金
- Deutsche Forschungsgemeinschaft [HE 5428]
We demonstrate methods to simultaneously acquire and evaluate the pattern of cell nuclei in the three cell layers of the vertebrate retina as an aspect of its functional morphology. 3D-position, shape and quantity of fluorescence-labelled cell nuclei are measured using laser scanning microscopy at several retinal locations, the pros and cons of single and two-photon excitation are compared. Subsequently topographies of all discriminable morphotypes are calculated via linear interpolation of local countings. In addition derived maps are calculated correlating density- and layer thickness-distributions to demonstrate the potential of 3D-morphometry in the retina. In the European anchovy Engraulis encrasicolus L (Engraulididae, Teleostei) the angular density of all involved cell types varies considerably with the location in the hemispherical coordinate system. All cells belonging to the photopic system show a density peak in the ventro-temporal quadrant, suggesting acute vision in the frontal binocular visual field. A second, less pronounced maximum is found nasally. (C) 2010 Elsevier B.V. All rights reserved.
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