期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 179, 期 2, 页码 191-200出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2009.01.023
关键词
Electrophysiology; Local field potential (LFP); Amperometry; Neurochemical; Acetylcholine; Simultaneous recording; Hippocampus
资金
- NARSAD
- NIH [5R01 DE011451-11]
- NIH NINDS [R33NS049534]
Simultaneous acquisition of in vivo electrophysiological and neurochemical information is essential for understanding how endogenous neurochemicals modulate the dynamics of brain activity. However, up to now such a task has rarely been accomplished due to the major technical challenge of operating two independent recording systems simultaneously in real-time. Here we propose a simpler solution for achieving this goal by using only a standard electrochemical technique - amperometry. To demonstrate its feasibility, we compared amperometric signals with simultaneously recorded local field potential (LFP) signals. We found that the high frequency component (HFC) of the amperometric signals did not reflect neurochemical fluctuations, but instead it resembled LFPs in several aspects, including: (1) coherent spectral fluctuations; (2) clear characterization of different brain states; (3) identical hippocampal theta depth profile. As such, our findings provide the first demonstration that both LFP and local neurochemical information can be simultaneously acquired from electrochemical sensors alone. (C) 2009 Elsevier B.V. All rights reserved.
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