期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 18, 页码 6405-6412出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5302-13.2014
关键词
dendritic spines; development; fragile X syndrome; spine dysgenesis; STED; super-resolution microscopy
资金
- Royal Society
- Medical Research Council
- Shirley Foundation
- Patrick Wild Centre
- Institut National de la Sante et de la Recherche Medicale
- Agence Nationale pour la Recherche
- Human Frontier Science Program
- France-BioImaging [ANR-10-INSB-04]
- Medical Research Council [MR/K014137/1] Funding Source: researchfish
- MRC [MR/K014137/1] Funding Source: UKRI
Dendritic spines are basic units of neuronal information processing and their structure is closely reflected in their function. Defects in synaptic development are common in neurodevelopmental disorders, making detailed knowledge of age-dependent changes in spine morphology essential for understanding disease mechanisms. However, little is known about the functionally important fine-morphological structures, such as spine necks, due to the limited spatial resolution of conventional light microscopy. Using stimulated emission depletion microscopy (STED), we examined spine morphology at the nanoscale during normal development in mice, and tested the hypothesis that it is impaired in a mouse model of fragile X syndrome (FXS). In contrast to common belief, we find that, in normal development, spine heads become smaller, while their necks become wider and shorter, indicating that synapse compartmentalization decreases substantially with age. In the mouse model of FXS, this developmental trajectory is largely intact, with only subtle differences that are dependent on age and brain region. Together, our findings challenge current dogmas of both normal spine development as well as spine dysgenesis in FXS, highlighting the importance of super-resolution imaging approaches for elucidating structure-function relationships of dendritic spines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据