Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: The study reveals the significant impact of Hofmeister effects on the activity and functions of biological molecules. It investigates the influence of anionic species treatment on the vesicular content and dynamics of exocytosis, showing distinct changes in the dynamics of vesicle release and opening after treatment with chaotropic anions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Laura M. Vecchio, Patricia Sullivan, Amy R. Dunn, Marie Kristel Bermejo, Rong Fu, Shababa T. Masoud, Emil Gregersen, Nikhil M. Urs, Reza Nazari, Poul Henning Jensen, Amy Ramsey, David S. Goldstein, Gary W. Miller, Ali Salahpour
Summary: TH plays a crucial role in Parkinson's disease, increasing TH activity leads to oxidative stress and accumulation of toxic dopamine metabolites in vivo, augmenting toxicity.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Tho Duc Khanh Nguyen, Lisa Mellander, Alicia Lork, Aurelien Thomen, Mai Philipsen, Michael E. Kurczy, Nhu T. N. Phan, Andrew G. Ewing
Summary: The traditional understanding of cell-to-cell communication was that full or none and kiss-and-run were the major exocytotic release modes, while the significance of partial release was not widely accepted.With the help of advanced imaging techniques and stable isotope labeling, this study provides direct evidence for partial release and demonstrates that it leads to measurable loss of transmitter in individual vesicles.
Article
Biology
Ricardo M. Santos, Anton Sirota
Summary: This study investigated a novel ChOx sensor for simultaneous measurement of COA and O-2, revealing that non-steady-state enzyme responses to O-2 result in phasic COA dynamics. The results suggest that probing phasic cholinergic signals under most behavioral paradigms with current ChOx biosensors is unfeasible.
Article
Biochemistry & Molecular Biology
Josette Zaklit, Alex Cabrera, Aaron Shaw, Rita Aoun, P. Thomas Vernier, Normand Leblanc, Gale L. Craviso
Summary: The study found that under certain conditions, ultra-short electric pulses can stimulate the release of catecholamines through exocytosis, with real-time observations showing that the release is Ca2+-dependent and the release events are relatively prolonged. Furthermore, the effectiveness of this stimulation method was demonstrated, indicating its potential as a novel electrostimulation modality for neurosecretion.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Juan Qiao, Deyu Wu, Yuying Song, Wenliang Ji, Qingwei Yue, Lanqun Mao, Li Qi
Summary: A new fluorescent probe was designed to simultaneously measure intracellular temperature and norepinephrine (NE) variations, revealing an increase in cell temperature and decrease in NE reuptake during antidepressant drug inhibition. This study provides insight into the underlying mechanisms of neurotransmitter signaling pathways, which could aid in the treatment of depression.
ANALYTICAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Phillip M. Mackie, Adithya Gopinath, Dominic M. Montas, Alyssa Nielsen, Aidan Smith, Rachel A. Nolan, Kaitlyn Runner, Stephanie M. Matt, John McNamee, Joshua E. Riklan, Kengo Adachi, Andria Doty, Adolfo Ramirez-Zamora, Long Yan, Peter J. Gaskill, Wolfgang J. Streit, Michael S. Okun, Habibeh Khoshbouei
Summary: Monocyte-derived macrophages play crucial roles in tissue homeostasis and diseases, regulated by various signaling molecules. Recent studies have shown the ability of biogenic amines to control macrophage functions, with a focus on the mechanisms surrounding immune cells remaining unclear. Investigating the functions of norepinephrine transporter (NET) and dopamine transporter (DAT) on human MDMs, researchers found that DAT may play a potential role in regulating innate immunity through an immunomodulatory mechanism mediated by LPS-induced dopamine reverse transport.
Article
Chemistry, Multidisciplinary
Chaoyi Gu, Andrew G. Ewing
Summary: A new technique was developed to simultaneously monitor and compare the storage and release of vesicular neurotransmitters in a neuroendocrine cell line. This technique allowed for the investigation of dynamic changes in vesicular transmitter content during chemical stimulation, providing insights into the reloading time scale of vesicles. Additionally, the effect of l-DOPA on vesicular storage alteration was studied, revealing a potential mechanism for plasticity in the exocytotic machinery.
Article
Neurosciences
Laura L. Grima, Marios C. Panayi, Oliver Harmson, Emilie C. J. Syed, Sanjay G. Manohar, Masud Husain, Mark E. Walton
Summary: Pharmacologically manipulating dopamine transmission affects animals' reward-driven behavioral control, especially in contexts where it is necessary to withhold actions to achieve beneficial outcomes. The balance of activity at NAcC D1Rs plays a key role in enabling animals to rapidly activate a focused, reward-seeking state.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Jarkko Johansson, Madelene Ericsson, Jan Axelsson, Sara af Bjerken, Ana Virel, Nina Karalija
Summary: Mapping the effects of drugs on the entire brain is crucial for understanding the neural mechanisms behind drug-related behaviors. Using PET, this study investigated the regional and temporal characteristics of amphetamine-induced dopamine release in the brain, revealing distinct responses in different brain regions.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Article
Psychology, Social
Jiaqi Yu, Shereen J. Chaudhry
Summary: Many studies have shown that expressing and experiencing gratitude has positive effects on individuals and relationships. This study examines the impact of culture and relational distance on people's expectations for gratitude. The findings suggest that expressing gratitude conveys exceeding relationship expectations and in collectivist cultures like China, people are less likely to express gratitude to close others.
JOURNAL OF PERSONALITY AND SOCIAL PSYCHOLOGY
(2023)
Article
Multidisciplinary Sciences
Silvia Maggi, Carlotta Bon, Stefano Gustincich, Valter Tucci, Raul R. Gainetdinov, Stefano Espinoza
Summary: Trace amine-associated receptor 5 (TAAR5) modulates cognitive processes, potentially affecting the number of errors and the rate of task improvement.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sevag Hamamah, Andras Hajnal, Mihai Covasa
Summary: Alterations in dopamine neurotransmission are associated with obesity and food preferences. OLETF rats with impaired satiation and obesity have a heightened preference for palatable sweet solutions, increased dopamine release, reduced D2R binding, and increased sensitivity to sucrose reward. Basal and stimulated motor activity in OLETF rats are affected by sucrose consumption, and DAT availability is reduced in the nucleus accumbens shell.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Jose A. Pino, Gabriel Nunez-Vivanco, Gabriela Hidalgo, Miguel Reyes Parada, Habibeh Khoshbouei, Gonzalo E. Torres
Summary: This study reveals that G protein beta gamma subunits can bind to the dopamine transporter (DAT) and activate DAT-mediated dopamine efflux, similar to the mechanism of action of psychostimulants like amphetamine (AMPH). Through a combination of computational biology, mutagenesis, biochemical, and functional assays, the amino acid residues within the 582-596 sequence of the DAT carboxy terminus involved in the DAT-G beta gamma interaction and G beta gamma-induced dopamine efflux were identified. Residues like R588 and F587 within the carboxy terminus of DAT play critical roles in the physical interaction between DAT and G beta gamma, influencing dopamine efflux.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Karolina Farrell, Armin Lak, Aman B. Saleem
Summary: Midbrain dopamine neurons encode reward prediction error signals to improve goal-directed navigation.