Article
Cell Biology
Torsten W. B. Goetz, Dmytro Puchkov, Veronika Lysiuk, Janine Luetzkendorf, Alexander G. Nikonenko, Christine Quentin, Martin Lehmann, Stephan J. Sigrist, Astrid G. Petzoldt
Summary: This study demonstrates that mutations in the small GTPase Rab2 lead to accumulation of presynaptic material in the neuronal cell body, resulting in neurotransmission deficits. This abnormal assembly process is dependent on Rab2-regulated protein export and sorting at the trans-Golgi, ultimately affecting neurotransmission.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Article
Multidisciplinary Sciences
Chiaki Ishii, Natsumi Shibano, Mio Yamazaki, Tomoki Arima, Yuna Kato, Yuki Ishii, Yo Shinoda, Yugo Fukazawa, Tetsushi Sadakata, Yoshitake Sano, Teiichi Furuichi
Summary: CAPS1 is a key molecule involved in vesicular exocytosis, but its role in synaptic plasticity and learning behavior remains unclear. Studies showed impaired synaptic plasticity and learning behavior in Caps1 cKO mice, with reduced docked vesicles in the hippocampal CA3 region and impaired long-term potentiation in CA1 and CA3 regions. These findings suggest that CAPS1 plays a crucial role in hippocampal synaptic release and plasticity, which is essential for hippocampus-associated learning.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Swapneeta S. Date, Peng Xu, Nathaniel L. Hepowit, Nicholas S. Diab, Jordan Best, Boyang Xie, Jiale Du, Eric R. Strieter, Lauren P. Jackson, Jason A. MacGurn, Todd R. Graham
Summary: This study reveals the role of COPI in SNARE trafficking and Golgi SNARE localization through ubiquitin recognition, and demonstrates how ubiquitination enhances the binding between Arf and COPI with Gos1.
Review
Biochemistry & Molecular Biology
Grace Kennedy, Olivia Gibson, Daire T. O'Hare, Ian G. Mills, Emma Evergren
Summary: Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a serine/threonine protein kinase involved in regulating energy homeostasis and cell growth. Its deregulation has implications in metabolic diseases and cancer. Recent research has explored new functions of CaMKK2, such as lipogenesis and Golgi vesicle trafficking. This review discusses the role of CaMKK2 in membrane trafficking mechanisms and its implications for disease.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Danette Kowal Seiler, Jesse C. Hay
Summary: Fluorescent proteins (FPs) have become essential tools for studying the constitutive secretory pathway in live cells since their discovery over fifty years ago. Genetically encoded chemical tags and innovative methods have also contributed to the understanding of sequential trafficking steps in the secretory pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Medicine, Research & Experimental
Weijie Yan, Jiahui Fan, Xia Zhang, Huimeng Song, Rongqi Wan, Wei Wang, Yanling Yin
Summary: The study investigated the impact of SNAP29 reduction on poststroke cognitive impairments using in vitro and in vivo ischemic models. It found that SNAP29 decrease was associated with synaptic dysfunction and cognitive deficits, without affecting neuronal survival. This suggests that strategies targeting SNAP29 could be effective in treating poststroke cognitive impairments.
Editorial Material
Cell Biology
Sisi Yang, Daniel A. Colon-Ramos
Summary: ATG-9 is involved in synaptic autophagy and exhibits activity-dependent exo-endocytosis at synapses. It is transported from the trans-Golgi network to synapses via the AP-3 complex in C. elegans. Disruption of the endocytosis pathway leads to abnormal ATG-9 accumulation and defects in activity-induced synaptic autophagy.
Article
Plant Sciences
Fan Wang, Zhijun Cheng, Jiachang Wang, Feng Zhang, Baocai Zhang, Sheng Luo, Cailin Lei, Tian Pan, Yongfei Wang, Yun Zhu, Min Wang, Weiwei Chen, Qibing Lin, Shanshan Zhu, Yihua Zhou, Zhichao Zhao, Jie Wang, Xiuping Guo, Xin Zhang, Ling Jiang, Yiqun Bao, Yulong Ren, Jianmin Wan
Summary: This study identified a rice mutant, dph1-1, with limited cell expansion, resulting in reduced plant height and smaller panicles and grains. The protein OsSCD2, homologous to Arabidopsis SCD2, was found to be associated with clathrin and involved in defective endocytosis and post-Golgi trafficking in the dph1-1 mutant. The mutant also displayed impaired cellulose synthesis. These findings suggest that OsSCD2 plays a crucial role in clathrin-related vesicular trafficking and is important for maintaining plant growth in rice.
Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Hsiao-Yun Cheng, Ting-Feng Hsiao, Tai-Wei Lin, Jia-Wei Hsu, Lien-Hung Huang, Chia-Jung Yu
Summary: ADP-ribosylation factors (Arfs) and Arf-like (Arl) GTPases are crucial regulators of intracellular vesicle trafficking and Golgi structure. In this study, it was found that ArfGAP1 can promote GTP hydrolysis of Arl1, affecting its function at the trans-Golgi network (TGN).
Article
Biochemistry & Molecular Biology
Roni Rahimi, Israel Malek, Tali Lerrer-Goldshtein, Yoav Elkis, Irit Shoval, Avi Jacob, Sally Shpungin, Uri Nir
Summary: The study investigates the regulatory role of TMF1 in GLUT4-mediated glucose uptake and insulin-directed functioning, showing that TMF1 is essential for the formation of insulin responsive, GLUT4-containing vesicles. Absence of TMF1 leads to impaired glucose uptake, resulting in hyperglycemia and decreased body weight in mice, indicating a novel effector in insulin-regulated glucose homeostasis.
Article
Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Article
Biology
Shataakshi Dube O'Neil, Bence Racz, Walter Evan Brown, Yudong Gao, Erik J. Soderblom, Ryohei Yasuda, Scott H. Soderling
Summary: The study uncovers the contribution of activity-dependent cytoskeletal signaling to presynaptic plasticity, revealing that altering Rac1 pathway negatively regulates synaptic vesicle replenishment bidirectionally sculpting short-term synaptic depression. Using three independent approaches to manipulate presynaptic Rac1 activity, the study demonstrates a previously unrecognized mechanism of actin-regulated short-term presynaptic plasticity that is conserved across excitatory and inhibitory terminals.
Review
Cell Biology
Daniel Ortuno-Sahagun, Julia Enterria-Rosales, Vanesa Izquierdo, Christian Grinan-Ferre, Merce Pallas, Celia Gonzalez-Castillo
Summary: This article discusses the important role of the miR-17-92 cluster in regulating cellular metabolism and autophagy, as well as its potential involvement in the pathogenesis of lysosomal storage diseases.
Review
Neurosciences
Ryota Fukaya, Rinako Miyano, Himawari Hirai, Takeshi Sakaba
Summary: Presynaptic plasticity refers to the activity-dependent changes in neurotransmitter release, playing a crucial role in modulating synaptic strength. Among these changes, presynaptic potentiation mediated by cyclic adenosine monophosphate (cAMP) is widely observed and implicated in learning and memory. Researchers have used hippocampal mossy fiber-CA3 pyramidal cell synapses as a model to investigate presynaptic potentiation, as the mechanisms underlying it can be dissected through direct presynaptic recordings. Recent advancements in super-resolution microscopy and flash-and-freeze electron microscopy have provided insights into the nanoscale localization of release site molecules and synaptic vesicles during potentiation, shedding light on the molecular mechanisms involved. In light of these findings, this study aims to propose plausible mechanisms underlying cAMP-mediated presynaptic potentiation.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Vikram Thimaradka, Jae Hoon Oh, Christina Heroven, A. Radu Aricescu, Michisuke Yuzaki, Tomonori Tamura, Itaru Hamachi
Summary: In this study, a new reactive peptide tag/probe pair system for site-specific covalent protein labeling was developed. The method relies on the recognition-driven reaction of a peptide tag and a molecular probe. The reactive profile and site-specificity of this method were characterized using model peptides and proteins in vitro, and the general utility for production of a nanobody-chemical probe conjugate without compromising its binding ability was demonstrated.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Neurosciences
Shigeo Miyata, Toshikazu Kakizaki, Kazuyuki Fujihara, Hideru Obinata, Touko Hirano, Junichi Nakai, Mika Tanaka, Shigeyoshi Itohara, Masahiko Watanabe, Kenji F. Tanaka, Manabu Abe, Kenji Sakimura, Yuchio Yanagawa
Summary: The study developed Gad1(tTA/STOP-tetO) biallelic knock-in mice, where GAD67 protein expression was reduced and GABA content decreased after doxycycline treatment, leading to emotional abnormalities in these mice. These findings provide valuable insights into the neurobiological mechanisms of emotional abnormalities related to psychiatric disorders.
Article
Behavioral Sciences
Makoto Wada, Kouji Takano, Masakazu Ide, Yoshitake Sano, Yo Shinoda, Teiichi Furuichi, Kenji Kansaku
Summary: This study aimed to elucidate the neural basis of the rubber tail illusion (RTI) response and its impairment in Caps2-KO mice by investigating c-Fos expression in both wild-type (WT) and Caps2-KO mice during the task. The results suggest that decreased c-Fos expression in the posterior parietal cortex may be related to impaired multisensory integrations in Caps2-KO mice, highlighting the importance of this brain region in body ownership illusions.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2021)
Article
Neurosciences
Teiichi Furuichi, Yuko Muto, Tetsushi Sadakata, Yumi Sato, Kanehiro Hayashi, Yoko Shiraishi-Yamaguchi, Yo Shinoda
Summary: This study elucidated the physiological roles of the Homer protein family in mouse cerebellar granule cells, revealing that the Homer2a N-terminal domain acts as a dominant negative protein to attenuate NMDAR-mediated excitotoxicity. Additionally, a novel short form N-terminal domain-containing Homer2, named Homer2e, was identified and found to be induced by apoptotic stimulation like ischemic brain injury. These findings suggest that long and short forms of Homer2 are involved in apoptosis of cerebellar granule cells.
Article
Multidisciplinary Sciences
Kansai Fukumitsu, Misato Kaneko, Teppo Maruyama, Chihiro Yoshihara, Arthur J. Huang, Thomas J. McHugh, Shigeyoshi Itohara, Minoru Tanaka, Kumi O. Kuroda
Summary: Social animals experience stress upon isolation and actively engage in contact with conspecifics. This study demonstrates that amylin-calcitonin receptor signaling in the medial preoptic area mediates affiliative social contacts among adult female mice. Isolation leads to active contact-seeking behavior and depressive-like behavior, along with a decrease in Amylin mRNA expression. Reuniting with peers induces physical contacts and a recovery of Amylin mRNA expression.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Juntan Li, Yo Shinoda, Shuhei Ogawa, Shunsuke Ikegaya, Shuo Li, Yukihiro Matsuyama, Kohji Sato, Satoru Yamagishi
Summary: FLRT2 protein and leucine-rich transmembrane (FLRT) proteins play crucial roles in various developmental processes and pathological conditions. The expression pattern of FLRT2 in CNS development was analyzed using Flrt2-LacZ knock-in mice, revealing dynamic changes in expression in different brain regions during development and after spinal cord injury.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Biology
Yuki Sakamoto, Anna Ishimoto, Yuuki Sakai, Moeko Sato, Ryuichi Nishihama, Konami Abe, Yoshitake Sano, Teiichi Furuichi, Hiroyuki Tsuji, Takayuki Kohchi, Sachihiro Matsunaga
Summary: Researchers developed an improved tissue clearing method, iTOMEI, for fluorescence microscopy of both plant and animal tissues. This method efficiently removes chlorophyll and autofluorescence signals while preserving the desired fluorescence protein signals.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Leon van Gurp, Leon Fodoulian, Daniel Oropeza, Kenichiro Furuyama, Eva Bru-Tari, Anh Nguyet Vu, John S. Kaddis, Ivan Rodriguez, Fabrizio Thorel, Pedro L. Herrera
Summary: The authors used single-cell transcriptomics meta-analysis to construct gene sets that accurately describe the identity of various human islet cells. These gene sets have proven effective in analyzing cell identity changes and their underlying genetic mechanisms, providing reliable tools for cell therapy and diabetes treatment.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Kento Ojima, Wataru Kakegawa, Tokiwa Yamasaki, Yuta Miura, Masayuki Itoh, Yukiko Michibata, Ryou Kubota, Tomohiro Doura, Eriko Miura, Hiroshi Nonaka, Seiya Mizuno, Satoru Takahashi, Michisuke Yuzaki, Itaru Hamachi, Shigeki Kiyonaka
Summary: The authors developed a chemogenetic method for direct activation of mGlu1 and demonstrated its use in mouse brain tissue.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yotaroh Sato, Miho Tsuyusaki, Hiromi Takahashi-Iwanaga, Rena Fujisawa, Atsushi Masamune, Shin Hamada, Ryotaro Matsumoto, Yu Tanaka, Yoichi Kakuta, Yumi Yamaguchi-Kabata, Tamio Furuse, Shigeharu Wakana, Takuya Shimura, Rika Kobayashi, Yo Shinoda, Ryo Goitsuka, So Maezawa, Tetsushi Sadakata, Yoshitake Sano, Teiichi Furuichi
Summary: CAPS2 plays a crucial role in the regulation of the pancreatic exocrine pathway and its deficiency is associated with the risk of pancreatic acinar cell pathology.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Neurosciences
Takahiro Aimi, Keiko Matsuda, Michisuke Yuzaki
Summary: Changes in neural activity induced by learning and novel environments can lead to the formation of new synapses in the adult brain. This study discovered that Purkinje cells in the cerebellum can be reinnervated by multiple climbing fibers through increased expression of the synaptic organizer C1ql1 or the receptor Bai3. The results indicate that C1ql1-Bai3 signaling, regulated by neuronal activity, may mediate climbing fiber synaptogenesis in mature Purkinje cells.
Article
Biochemistry & Molecular Biology
Chika Saegusa, Wataru Kakegawa, Eriko Miura, Takahiro Aimi, Sachiyo Mogi, Tatsuhiko Harada, Taku Yamashita, Michisuke Yuzaki, Masato Fujioka
Summary: This study investigated the expression and function of Bai3, an adhesion G-protein-coupled receptor, in the cochlea. The results showed that Bai3 plays a crucial role in the development and maintenance of pillar cells, and its loss can lead to abnormal formation of pillar cells and impaired hearing function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Takumi Katsuzawa, Kohei Kujirai, Shinji Kamisuki, Yo Shinoda
Summary: Malignant meningioma has a poor prognosis and lacks effective therapies. Avenaciolide, a water-insoluble organic compound derived from Aspergillus avenaceus, was found to exhibit effective anticancer activity in a human malignant meningioma cell line. This activity was mediated by the induction of reactive oxygen species-induced apoptosis, potentially due to mitochondrial dysfunction. These findings suggest that avenaciolide holds potential as a therapeutic drug for malignant meningioma.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Toxicology
Yo Shinoda, Yuta Yamada, Eiko Yoshida, Tsutomu Takahashi, Yayoi Tsuneoka, Komyo Eto, Toshiyuki Kaji, Yasuyuki Fujiwara
Summary: The study found that pain sensation is the sensory modality most susceptible to MeHg toxicity, while other sensory modalities are not affected by MeHg exposure. This sensitivity is reversible following discontinuation of exposure.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2021)
