Article
Cell Biology
Guanhua Bai, Yu Wang, Mingjie Zhang
Summary: Inhibitory synapses, although lacking prominent PSDs, have been found to contain thin sheet-like PSDs. Gephyrin protein, when in complex with neurotransmitter receptors, can form condensed molecular assemblies through phase separation. The formation of iPSD condensates is regulated by phosphorylation of specific residues in gephyrin or binding of target proteins to the linker domain.
Article
Biochemistry & Molecular Biology
Victoria R. Sanders, Aaron Sweeney, Maya Topf, Neil S. Millar
Summary: Quinolone antibiotics can disrupt bacterial DNA synthesis and also act as inhibitors of pentameric ligand-gated ion channels, including the alpha 4 beta 2 nicotinic acetylcholine receptor. These antibiotics selectively inhibit different stoichiometries of the alpha 4 beta 2 receptor, which can result in different pharmacological properties.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chenlu Yu, Hendrik F. P. Runge, Antara Mukhopadhyay, Gerd Zolles, Maximilian H. Ulbrich
Summary: Using single-molecule imaging, this study investigates the binding of auxiliary subunits gamma-2 and GSG1L to the AMPA receptor. The results show that both subunits compete for binding sites on the receptor and have similar binding affinities, suggesting dynamic changes in receptor composition under native conditions.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Pharmacology & Pharmacy
Sandra M. Martin-Guerrero, Paula Alonso, Alba Iglesias, Marta Cimadevila, Jose Brea, M. Isabel Loza, Pedro Casado, David Martin-Oliva, Pedro R. Cutillas, Javier Gonzalez-Maeso, Juan F. Lopez-Gimenez
Summary: Antipsychotic drugs induce phosphorylation of multiple pathways downstream of 5-HT2AR, and the single nucleotide polymorphism encoding 5-HT2AR-H452Y affects these drug-induced phosphorylation-dependent signaling networks.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Neurosciences
Hailong Yu, Xiao-Chen Bai, Weiwei Wang
Summary: The strychnine-sensitive pentameric glycine receptor plays a crucial role in fast inhibitory neurotransmission in the mammalian nervous system. The study found that a single beta subunit in each GlyR is responsible for the characteristic electrophysiological properties of heteromeric GlyR.
Review
Pharmacology & Pharmacy
Anna Pittaluga
Summary: This review summarizes the results from synaptosome studies over the past 40 years, supporting the existence of auto and hetero NMDA receptors controlling the release of transmitters in the CNS of mammals. Additionally, immunochemical studies in isolated nerve endings confirm these functional observations.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Rodolfo Ciuffa, Federico Uliana, Jonathan Mannion, Martin Mehnert, Tencho Tenev, Cathy Marulli, Ari Satanowski, Lena Maria Leone Keller, Pilar Natalia Rodilla Ramirez, Alessandro Ori, Matthias Gstaiger, Pascal Meier, Ruedi Aebersold
Summary: Protein-protein interactions (PPIs) are the main mode of proteome organization in cells. Traditional PPI networks lack contextual information. Generating context-dependent PPI networks is necessary for structural and systems-level modeling, but remains challenging. In this study, an experimental/computational strategy is described to achieve contextual modeling of PPIs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Xuerui Yang, Xi Ding, Lei Zhou, Qing Zhao, Yuefei Ji, Xingbao Wang, Jean-Marc Chovelon, Guangli Xiu
Summary: The study found that unactivated PMS can directly oxidize TMP through a non-radical process, promoting PMS decay, with alkaline conditions favoring TMP degradation. The two amine groups on the pyrimidine ring were identified as reactive sites, and the oxidation products were stable.
Article
Multidisciplinary Sciences
Tomoki Ota, Akinobu Senoo, Masumi Shirakawa, Hiroshi Nonaka, Yutaro Saito, Sho Ito, Go Ueno, Satoru Nagatoishi, Kouhei Tsumoto, Shinsuke Sando
Summary: Bile acids play a crucial role in lipid digestion and absorption in the small bowel, as well as regulating their own metabolism and impacting other biological systems. The study suggests that secondary bile acid conjugates may act as modulators of serine hydroxymethyltransferase activity.
Article
Biochemistry & Molecular Biology
Patrick Lambert-Lanteigne, Adrian Young, Chantal Autexier
Summary: In human cancer cells, cellular immortalization relies on the maintenance of telomere length by telomerase. The interaction between telomere protein TPP1 and the telomerase reverse transcriptase (hTERT)'s N-terminal domain, TEN, regulates telomerase recruitment to telomeres. The hTERT 'insertion in fingers domain' (IFD) is involved in this process. The IFD domain can interact with TPP1, but its presence is not essential for the interaction. Several conserved residues in the IFD-TRAP region regulate telomerase recruitment and cell immortalization, and mutations in these residues compromise the interaction between the IFD domain and TPP1.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Agronomy
Toshifumi Nakao, Shinichi Banba
Summary: The research demonstrates the differential antagonist activities of meta-diamides on insect and human receptors by studying the effects of equivalent mutations in human GABA receptors. This study reveals the underlying mechanisms of selectivity of meta-diamides between insect RDL and human GABA and glycine receptors.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lisa Desiree Westrich, Vincent Leon Gotsmann, Claudia Herkt, Fabian Ries, Tanja Kazek, Raphael Troesch, Laura Armbruster, Jens Stephan Muehlenbeck, Silvia Ramundo, Joerg Nickelsen, Iris Finkemeier, Markus Wirtz, Zuzana Storchova, Markus Raeschle, Felix Willmund
Summary: The research established a ribosome affinity purification method to uncover potential ribosome-associated proteins in chloroplasts, revealing interactors of the holo complex and the two subunits. This broad set of ribosome interactors highlights the potential to regulate chloroplast gene expression through protein synthesis.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xiangdong Zheng, Zhengshan Hu, Huan Li, Jian Yang
Summary: The cryo-EM structure of the human cone photoreceptor CNG channel reveals an unusual subunit stoichiometry and unique structural features due to a distinct subunit, contributing to the understanding of cone CNG channel physiology and channelopathies. This study provides insight into the critical role of CNGB3 in shaping cone photoresponses, highlighting the importance of its structural features and disease mutations in determining visual function.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Tiina Heinonen, Thomas Flegel, Hanna Mueller, Alexandra Kehl, Sruthi Hundi, Kaspar Matiasek, Andrea Fischer, Jonas Donner, Oliver P. Forman, Hannes Lohi, Marjo K. Hytoenen
Summary: In this study, a Miniature Australian Shepherd family with symptoms similar to hereditary hyperekplexia in humans was reported. Whole genome sequence data analysis revealed a 36-bp deletion in the GLRA1 gene, which was found to be completely associated with the disease in pedigree samples and additional cohorts of related dog breeds. This study establishes a spontaneous large animal disease model for human hereditary hyperekplexia by associating a variant in the canine GLRA1 gene with the disorder for the first time.
Article
Multidisciplinary Sciences
Hongtao Zhu, Eric Gouaux
Summary: The study presents the cryo-electron microscopy structures of native GlyRs, revealing a predominant subunit stoichiometry of 4 alpha:1 beta and unique structural features at the beta(+)-alpha(-) interface. The presence of a phenylalanine residue in the beta-subunit disrupts the canonical picrotoxin site, impacting receptor symmetry and ion channel pharmacology. Furthermore, incomplete receptor complexes and partially assembled alpha-trimers and alpha-tetramers are identified through structural elucidation.
Article
Chemistry, Multidisciplinary
Noernie Danne, Mijin Kim, Antoine G. Godin, Hyejin Kwon, Zhenghong Gao, Xiaojian Wu, Nicolai F. Hartmann, Stephen K. Doorn, Brahim Lounis, YuHuang Wang, Laurent Cognet
Article
Neurosciences
Nela Durisic, Angelo Keramidas, Christine L. Dixon, Joseph W. Lynch
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2018)
Article
Clinical Neurology
Annemarie Dedek, Jian Xu, Chaya M. Kandegedara, Louis-Etienne Lorenzo, Antoine G. Godin, Yves De Koninck, Paul J. Lombroso, Eve C. Tsai, Michael E. Hildebrand
Article
Biophysics
Daniel J. Foust, Antoine G. Godin, Alessandro Ustione, Paul W. Wiseman, David W. Piston
BIOPHYSICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Antoine G. Godin, Antonio Setaro, Morgane Gandil, Rainer Haag, Mohsen Adeli, Stephanie Reich, Laurent Cognet
Article
Multidisciplinary Sciences
Louis-Etienne Lorenzo, Antoine G. Godin, Francesco Ferrini, Karine Bachand, Isabel Plasencia-Fernandez, Simon Labrecque, Alexandre A. Girard, Dominic Boudreau, Irenej Kianicka, Martin Gagnon, Nicolas Doyon, Alfredo Ribeiro-da-Silva, Yves De Koninck
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Anh T. Nguyen, Feng Gao, Dustin Baucom, Colin D. Heyes
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Neurosciences
Melina Papalampropoulou-Tsiridou, Simon Labrecque, Antoine G. Godin, Yves De Koninck, Feng Wang
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Francesco Ferrini, Jimena Perez-Sanchez, Samuel Ferland, Louis-Etienne Lorenzo, Antoine G. Godin, Isabel Plasencia-Fernandez, Martin Cottet, Annie Castonguay, Feng Wang, Chiara Salio, Nicolas Doyon, Adalberto Merighi, Yves De Koninck
NATURE COMMUNICATIONS
(2020)
Article
Biophysics
Dustin R. Baucom, Mercede Furr, Vivek Govind Kumar, Patience Okoto, James L. Losey, Ralph L. Henry, Mahmoud Moradi, Thallapuranam Krishnaswamy Suresh Kumar, Colin D. Heyes
Summary: The study reveals that Alb3-Cterm contains transient secondary structure in certain motifs, which may play a crucial role in its recognition and binding to the transit complex. The excellent agreement between experimental and computational data allowed for the successful prediction of the apparent unfolding free energy of this secondary structure.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Mamello Mohale, Ravi Kumar Gundampati, Thallapuranam Krishnaswamy Suresh Kumar, Colin D. Heyes
Summary: This study investigated the binding of human fibroblast growth factor-1 (hFGF1) to its receptor and heparin, as well as the application of fluorescence imaging in studying protein interactions. Two different regions of the protein were labeled to study the effect of dye structure on labeling efficiency. High or low flexibility in either the protein labeling site or the dye structure affected the labeling efficiency, while too much rigidity resulted in protein aggregation. Importantly, site-specific labeling maintained biological activity.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Clinical Neurology
Annemarie Dedek, Jian Xu, Louis-Etienne Lorenzo, Antoine G. Godin, Chaya M. Kandegedara, Genevieve Glavina, Jeffrey A. Landrigan, Paul J. Lombroso, Yves De Koninck, Eve C. Tsai, Michael E. Hildebrand
Summary: This study discovered sexual dimorphism in a central neuronal mechanism of chronic pain, providing a foundational step towards a better understanding and treatment of pain in both sexes.
Article
Chemistry, Medicinal
Mitchell Benton, Mercede Furr, Vivek Govind Kumar, Adithya Polasa, Feng Gao, Colin David Heyes, Thallapuranam Krishnaswamy Suresh Kumar, Mahmoud Moradi
Summary: The novel protein cpSRP43 plays a unique role in the post-translational chloroplast signal recognition particle (cpSRP) targeting pathway. It acts as a chaperone to LHCPs and can dissolve LHCP aggregates without external energy input. Through computational and experimental techniques, the study reveals that cpSRP43 is simultaneously highly stable and flexible, with different conformations and dynamics when bound to cpSRP54.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Multidisciplinary Sciences
Tristan P. Wallis, Anmin Jiang, Kyle Young, Huiyi Hou, Kye Kudo, Alex J. McCann, Nela Durisic, Merja Joensuu, Dietmar Oelz, Hien Nguyen, Rachel S. Gormal, Frederic A. Meunier
Summary: Single-molecule localization microscopy techniques are essential for understanding the organization of protein clusters at the nanometer scale in living cells. In this study, the R-tree spatial indexing algorithm was used to determine the membership of individual molecular trajectories in nanoclusters. By extending the spatial indexing into the time dimension, spatiotemporal clusters were identified, revealing the dynamics of neuroexocytosis. The findings have been incorporated into a free and open-source Python graphic user interface called NASTIC.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Xiaorong Pan, Jie Li, Wei Li, Haofei Wang, Nela Durisic, Zhenyu Li, Yu Feng, Yifan Liu, Chun-Xia Zhao, Tong Wang
Summary: Researchers have successfully modeled DAI and monitored instant cellular responses using an "axon-on-a-chip" (AoC) model. They found that the severity of DAI is correlated with the intensity of the mechanical impact. Additionally, they observed periodic disruption of the axonal cytoskeleton after the impact. These findings provide insights into the early mechanisms of DAI and offer a platform for screening effective treatments for TBI.
