4.7 Article

Neuronal Glutamate Transporters Regulate Glial Excitatory Transmission

期刊

JOURNAL OF NEUROSCIENCE
卷 32, 期 5, 页码 1528-1535

出版社

SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5232-11.2012

关键词

-

资金

  1. National Institutes of Health [NS065920]

向作者/读者索取更多资源

In the CNS, excitatory amino acid transporters (EAATs) localized to neurons and glia terminate the actions of synaptically released glutamate. Whereas glial transporters are primarily responsible for maintaining low ambient levels of extracellular glutamate, neuronal transporters have additional roles in shaping excitatory synaptic transmission. Here we test the hypothesis that the expression level of the Purkinje cell (PC)-specific transporter, EAAT4, near parallel fiber (PF) release sites controls the extrasynaptic glutamate concentration transient following synaptic stimulation. Expression of EAAT4 follows a parasagittal banding pattern that allows us to compare regions of high and low EAAT4-expressing PCs. Using EAAT4 promoter-driven eGFP reporter mice together with pharmacology and genetic deletion, we show that the level of neuronal transporter expression influences extrasynaptic transmission from PFs to adjacent Bergmann glia (BG). Surprisingly, a twofold difference in functional EAAT4 levels is sufficient to alter signaling to BG, although EAAT4 may only be responsible for removing a fraction of released glutamate. These results demonstrate that physiological regulation of neuronal transporter expression can alter extrasynaptic neuroglial signaling.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Neurosciences

Constitutive and Synaptic Activation of GIRK Channels Differentiates Mature and Newborn Dentate Granule Cells

Jose Carlos Gonzalez, S. Alisha Epps, Sean J. Markwardt, Jacques I. Wadiche, Linda Overstreet-Wadiche

JOURNAL OF NEUROSCIENCE (2018)

Article Chemistry, Multidisciplinary

Design of a Highly Bistable Photoswitchable Tethered Ligand for Rapid and Sustained Manipulation of Neurotransmission

Wan-Chen Lin, Ming-Chi Tsai, Rajit Rajappa, Richard H. Kramer

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Neurosciences

α-Synuclein fibril-induced paradoxical structural and functional defects in hippocampal neurons

Jessica M. Froula, Benjamin W. Henderson, Jose Carlos Gonzalez, Jada H. Vaden, John W. Mclean, Yumei Wu, Gokulakrishna Banumurthy, Linda Overstreet-Wadiche, Jeremy H. Herskowitz, Laura A. Volpicelli-Daley

ACTA NEUROPATHOLOGICA COMMUNICATIONS (2018)

Article Multidisciplinary Sciences

NeuroD2 controls inhibitory circuit formation in the molecular layer of the cerebellum

Alexander Pieper, Stephanie Rudolph, Georg L. Wieser, Tilmann Goetze, Hendrik Miessner, Tomoko Yonemasu, Kuo Yan, Iva Tzvetanova, Bettina Duverge Castillo, Ulli Bode, Ingo Bormuth, Jacques I. Wadiche, Markus H. Schwab, Sandra Goebbels

SCIENTIFIC REPORTS (2019)

Article Biology

The readily-releasable pool dynamically regulates multivesicular release

Jada H. Vaden, Gokulakrishna Banumurthy, Eugeny S. Gusarevich, Linda Overstreet-Wadiche, Jacques I. Wadiche

Article Neurosciences

A Role for PGC-1α in Transcription and Excitability of Neocortical and Hippocampal Excitatory Neurons

L. J. McMeekin, A. F. Bartley, A. S. Bohannon, E. W. Adlaf, T. van Groen, S. M. Boas, S. N. Fox, P. J. Detloff, D. K. Crossman, L. S. Overstreet-Wadiche, J. J. Hablitz, L. E. Dobrunz, R. M. Cowell

NEUROSCIENCE (2020)

Article Cell Biology

RGS6 Mediates Effects of Voluntary Running on Adult Hippocampal Neurogenesis

Yu Gao, Minjie Shen, Jose Carlos Gonzalez, Qiping Dong, Sudharsan Kannan, Johnson T. Hoang, Brian E. Eisinger, Jyotsna Pandey, Sahar Javadi, Qiang Chang, Daifeng Wang, Linda Overstreet-Wadiche, Xinyu Zhao

CELL REPORTS (2020)

Article Biology

Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells

Ryan J. Vaden, Jose Carlos Gonzalez, Ming-Chi Tsai, Anastasia J. Niver, Allison R. Fusilier, Chelsea M. Griffith, Richard H. Kramer, Jacques I. Wadiche, Linda Overstreet-Wadiche

Article Neurosciences

Relocation of an Extrasynaptic GABAA Receptor to Inhibitory Synapses Freezes Excitatory Synaptic Strength and Preserves Memory

Christopher M. Davenport, Rajit Rajappa, Ljudmila Katchan, Charlotte R. Taylor, Ming-Chi Tsai, Caleb M. Smith, Johannes W. de Jong, Don B. Arnold, Stephan Lammel, Richard H. Kramer

Summary: The study uncovers a hidden form of inhibitory synaptic plasticity that prevents the accumulation of excitatory long-term potentiation (LTP). Induction of excitatory LTP relocates α5-GABARs to inhibitory synapses, interrupting further LTP induction. This dual plasticity between inhibitory and excitatory synapses may be a critical early step in memory preservation.

NEURON (2021)

Article Neurosciences

Climbing Fiber-Mediated Spillover Transmission to Interneurons Is Regulated by EAAT4

Shreya Malhotra, Gokulakrishna Banumurthy, Reagan L. Pennock, Jada H. Vaden, Izumi Sugihara, Linda Overstreet-Wadiche, Jacques Wadiche

Summary: Neurotransmitter spillover, a form of communication not easily predicted by anatomy, is regulated by the patterned expression of the excitatory amino acid transporter 4 in the cerebellum. Through experiments using Aldolase C-Venus knock-in mice, researchers found that heterogeneity in EAAT4 expression is the primary determinant of differential spillover between neuronal microzones. This study highlights how neuronal glutamate transporters limit extrasynaptic transmission in a non-cell-autonomous manner and provides new insights into the functional specialization of cerebellar microzones.

JOURNAL OF NEUROSCIENCE (2021)

Article Health Care Sciences & Services

Machine Learning-Based Text Analysis to Predict Severely Injured Patients in Emergency Medical Dispatch: Model Development and Validation

Kuan-Chen Chin, Yu-Chia Cheng, Jen-Tang Sun, Chih-Yen Ou, Chun-Hua Hu, Ming-Chi Tsai, Matthew Huei-Ming Ma, Wen-Chu Chiang, Albert Y. Chen

Summary: A machine learning-based model, called the PAMT model, was developed to predict severe road accident trauma. The study found that the accuracy of the PAMT model is similar to that of the participating dispatchers, but it may provide higher accuracy when the dispatchers lack confidence in their judgments.

JOURNAL OF MEDICAL INTERNET RESEARCH (2022)

Article Cell Biology

Circadian regulation of dentate gyrus excitability mediated by G-protein signaling

Jose Carlos Gonzalez, Haeun Lee, Angela M. Vincent, Angela L. Hill, Lacy K. Goode, Gwendalyn D. King, Karen L. Gamble, I. Wadiche, Jacques I. Wadiche, Linda Overstreet-Wadiche

Summary: The central circadian regulator in the suprachiasmatic nucleus controls the diurnal spiking rhythm through molecular clock genes regulated membrane excitability, transmitting time information. Most brain regions, including the hippocampus, possess similar intrinsic circadian transcription machinery, but whether they generate oscillations of membrane properties is uncertain. This study demonstrates that mouse dentate granule neurons exhibit a 24-hour oscillation in intrinsic excitability which regulates spiking probability. The ciradian transcriptional machinery coordinates ion channels through G-protein signaling to regulate intrinsic excitability, providing a potential mechanism for cell-autonomous oscillations.

CELL REPORTS (2023)

暂无数据