Review
Physiology
Victoria Maneu, Ricardo Borges, Luis Gandia, Antonio G. Garcia
Summary: This article reviews the evolution of knowledge on the biology of the adrenal medulla gland and its chromaffin cells over the past two centuries. It is divided into two periods: before 1982 and from 1982 to 2022. The article assesses the important breakthroughs and advancements made in the field of adrenal medulla gland and chromaffin cells.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: This study examined the temporal and concentration effects of chemical stimulation on exocytotic molecular plasticity. The results showed that catecholamine release and release dynamics depended on the concentration of the stimulant solution. Increased release was observed at 30 and 50 mM stimulation, while decreased release was observed at 100 mM stimulation. These findings suggest a possible link between stimulation intensity and synaptic (or adrenal) plasticity.
Article
Multidisciplinary Sciences
Sarah B. Nyenhuis, Nakul Karandikar, Volker Kiessling, Alex J. B. Kreutzberger, Anusa Thapa, Binyong Liang, Lukas K. Tamm, David S. Cafiso
Summary: The study shows that an arginine-containing region in synaptotagmin 1 controls the expansion of the fusion pore, regulating the concentration of released neurotransmitters. Different faces of the C2B domain play distinct roles in regulating neurotransmitter release, with the expansion of the fusion pore mediated by membrane contact of the C2B arginine apex.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Sebastien Houy, Joana S. Martins, Noa Lipstein, Jakob Balslev Sorensen
Summary: This study investigated the relationship between phorbolesters and Munc13-1 and ubMunc13-2, and found that phorbolesters only stimulated secretion when ubMunc13-2 was dominant. The results showed that DAG/phorbolesters, ubMunc13-2, and Syt7 are important factors for stimulating dense-core vesicle priming.
Article
Cell Biology
Ryan Khounlo, Brenden J. D. Hawk, Tung-Mei Khu, Gyeongji Yoo, Nam Ki Lee, Josh Pierson, Yeon-Kyun Shin
Summary: Alpha-synuclein plays a crucial role in promoting the expansion of fusion pores by increasing the probability and duration of large pores, specifically through its interaction with the membrane. This finding contributes to a better understanding of neurotransmitter release mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Eva-Maria Blumrich, Jessica C. Nicholson-Fish, Marie Pronot, Elizabeth C. Davenport, Dominic Kurian, Adam Cole, Karen J. Smillie, Michael A. Cousin
Summary: Phosphatidylinositol 4-kinase IIa (PI4KIIa) is essential for activity-dependent bulk endocytosis (ADBE) in primary neuronal cultures. The phosphorylation of Ser-47 by glycogen synthase kinase 3(3 (GSK3(3) is crucial for ADBE. Phosphomimetic PI4KIIa interacts with specific presynaptic molecules, including AGAP2 and CAMKV, which are also essential for ADBE when depleted in neurons.
Article
Cell Biology
Kumari Kamalesh, Nadav Scher, Tom Biton, Eyal D. Schejter, Ben-Zion Shilo, Ori Avinoam
Summary: This study found that vesicular membranes prevent fusion with the apical surface through actomyosin-mediated folding and retention, and isolate the membranes by crumpling to limit protein and lipid diffusion. Actomyosin contraction and membrane crumpling play key roles in recruiting endocytosis.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Fabio L. Urbina, Shalini Menon, Dennis Goldfarb, Reginald Edwards, M. Ben Major, Patrick Brennwald, Stephanie L. Gupton
Summary: During neuronal development, membrane expansion and exocytosis play crucial roles in morphogenesis. Different fusion modes, such as FVF and KNR, have been identified in developing neurons, with distinct behaviors of fusion pores impacting membrane incorporation and cargo secretion. Further research shows that TRIM67 promotes FVF-like exocytosis by regulating SNARE complexes, highlighting the importance of specific fusion modes in neuronal morphogenesis.
Article
Cell Biology
Barbara Pernaute, Salvador Perez-Montero, Juan Miguel Sanchez Nieto, Aida Di Gregorio, Ana Lima, Katerina Lawlor, Sarah Bowling, Gianmaria Liccardi, Alejandra Tomas, Pascal Meier, Hiromi Sesaki, Guy A. Rutter, Ivana Barbaric, Tristan A. Rodriguez
Summary: This study reveals the importance of mitochondrial apoptotic regulation in early mouse development. The researchers found that when cells exit naive pluripotency and start differentiation, the mitochondrial apoptotic threshold decreases, making the cells more sensitive to cell death. This enhanced apoptotic response is caused by a reduction in mitochondrial fission and a decrease in mitophagy levels.
DEVELOPMENTAL CELL
(2022)
Article
Genetics & Heredity
Manisha Sinha, Ankita Shree, Kunal Singh, Kamal Kumar, Shreenivas Kumar Singh, Vimlesh Kumar, Praveen Kumar Verma
Summary: ArF-BAR protein in the filamentous fungus Ascochyta rabiei regulates endocytosis and actin dynamics in hyphal growth, playing a crucial role in fungal virulence on the host plant. Its functional significance is highlighted by its regulation through a stress-induced transcription factor and involvement in effector secretion and actin cytoskeleton regulation. This study sheds light on the importance of F-BAR domain proteins in fungal pathophysiology, especially in the context of plant infection.
Article
Pharmacology & Pharmacy
Sohrab Rahmani, Ali Roohbakhsh, Gholamreza Karimi
Summary: Mitochondria are dynamic organelles that undergo fission and fusion processes to regulate their integrity and bioenergetics. Excessive mitochondrial fission can lead to structural changes, dysfunction, and cell damage. Accumulating evidence shows that mitochondrial dynamics play a role in neurodegenerative and cardiovascular diseases, as well as other diseases.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Dinara Bulgari, Samantha L. Cavolo, Brigitte F. Schmidt, Katherine Buchan, Marcel P. Bruchez, David L. Deitcher, Edwin S. Levitan
Summary: Neuronal dense-core vesicles (DCVs) release their contents through kiss-and-run exocytosis at the Drosophila neuromuscular junction. cAMP-induced extra fusions with dilating fusion pores allow for the release of large cargoes that cannot pass through narrower fusion pores. These findings indicate that the fusion pore acts as a variable filter, determining the composition of proteins released at the synapse.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Analytical
Gregory S. McCarty, Lars E. Dunaway, J. Dylan Denison, Leslie A. Sombers
Summary: This study investigates the reasons why released neurotransmitters can escape detection in amperometric measurements. The results reveal that both diffusional loss and the action of the norepinephrine transporter contribute to the undetected neurotransmitter. This has important implications for understanding the mechanisms of neurotransmitter release.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Delphine Mion, Louis Bunel, Paul Heo, Frederic Pincet
Summary: Membrane fusion is an energy-consuming process that involves the formation of SNAREpins to bridge vesicle and target membranes and provide the necessary energy. Three energy barriers have been identified and can be overcome by multiple SNAREpins acting in concert.
Article
Cell Biology
Che-Wei Chang, Yu-Tien Hsiao, Katherine S. Scheuer, Meyer B. Jackson
Summary: This study introduces a new method to analyze amperometry data and improve the classification of exocytosis fusion modes. The study discovers that fusion pore lifetimes are dependent on vesicle size and are regulated by the secretory vesicle protein synaptophysin. Incorporating vesicle size into the analysis provides a better understanding of the kinetics and distribution of exocytosis.
Article
Cell Biology
Prattana Samasilp, Kyle Lopin, Shyue-An Chan, Rajesh Ramachandran, Corey Smith
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2014)
Meeting Abstract
Biophysics
Barbara A. Kuri, Shyue-An Chan, Corey Smith
BIOPHYSICAL JOURNAL
(2009)
Meeting Abstract
Biophysics
Prattana Samasilp, Shyue-An Chan, Corey B. Smith
BIOPHYSICAL JOURNAL
(2012)
Article
Cell Biology
Shyue-An Chan, Jacqueline Hill, Corey Smith
Article
Cell Biology
Shyue-An Chan, Bryan Doreian, Corey Smith
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2010)
Article
Endocrinology & Metabolism
N. Stroth, B. A. Kuri, T. Mustafa, S. -A. Chan, C. B. Smith, L. E. Eiden
Article
Neurosciences
Thomas Ladas, Shyue-An Chan, Michael Ogier, Corey Smith, David M. Katz
EUROPEAN JOURNAL OF NEUROSCIENCE
(2009)
Article
Biochemistry & Molecular Biology
Jacqueline Hill, Shyue-An Chan, Barbara Kuri, Corey Smith
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Barbara A. Kuri, Shyue-An Chan, Corey B. Smith
JOURNAL OF NEUROCHEMISTRY
(2009)
Article
Cardiac & Cardiovascular Systems
Shyue-An Chan, Marmar Vaseghi, Nicholas Kluge, Kalyanam Shivkumar, Jeffrey L. Ardell, Corey Smith
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Nicholas Kluge, Michael Dacey, Joseph Hadaya, Kalyanam Shivkumar, Shyue-An Chan, Jeffrey L. Ardell, Corey Smith
Summary: Sympathetic control of regional cardiac function is primarily mediated through norepinephrine (NE) and neuropeptide Y (NPY) release. Elevated sympathetic stress can lead to increased NE and NPY release, implicated in various cardiovascular disorders. Current methods for detecting NPY in myocardium are limited, but a new minimally invasive method using a capacitive immunoprobe shows promise in providing high-resolution, multichannel measurement of NPY release in vivo. This approach can capture rapid and local changes in neurotransmitter profiles in response to sympathetic cardiac stressors, with potential future applications for real-time intraoperative monitoring.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Kyle Wolf, Georgy Zarkua, Shyue-An Chan, Arun Sridhar, Corey Smith
PHYSIOLOGICAL REPORTS
(2016)
Meeting Abstract
Biophysics
Jacqueline Hill Tudor, Shyue-An Chan, Corey Smith
BIOPHYSICAL JOURNAL
(2011)
Meeting Abstract
Biophysics
Prattana Samasilp, Bryan Doreian, Shyue-An Chan, Corey Smith
BIOPHYSICAL JOURNAL
(2011)