Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Erika Cosset, Youssef Hibaoui, Sten Ilmjarv, Pierre-Yves Dietrich, Caroline Tapparel, Karl-Heinz Krause
Summary: This study presents a standardized protocol for differentiating human embryonic stem cells into engineered neural tissue enriched with motor neurons, and highlights the role of motor neurons in poliovirus infection within these tissues. The research also reveals the molecular events occurring during PV-3 infection in the motor neurons and emphasizes the modulation of a set of genes involved in the EGR-EP300 complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Anastasiya Mamaeva, Olga Krasnova, Irina Khvorova, Konstantin Kozlov, Vitaly Gursky, Maria Samsonova, Olga Tikhonova, Irina Neganova
Summary: Human pluripotent stem cells have great potential for research and therapy, but their maintenance in culture requires careful control of their pluripotent and clonal status. In this study, we developed a classifier using a convolutional neural network to analyze images of human embryonic stem cell colonies and predict their morphological phenotypes associated with pluripotency and clonality. The classifier showed high accuracy in predicting phenotype (89%) and the spatial scale of around 144 μm was found to be most informative for classification. Proteomic analysis also revealed molecular differences between cells with different phenotypes. This non-invasive automated analysis approach could be valuable for identifying developmental anomalies in pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Biochemistry & Molecular Biology
Seung-Bin Na, Bong-Jong Seo, Tae-Kyung Hong, Seung-Yeon Oh, Yean-Ju Hong, Jae-Hoon Song, Sang-Jun Uhm, Kwonho Hong, Jeong-Tae Do
Summary: This study investigated the impact of knocking out the key regulator of mitochondrial fission, the Dnm1l gene, on neural stem cells (NSCs). The results suggest that Dnm1l deficiency leads to impaired self-renewal ability and accelerated cellular aging in NSCs, possibly due to mitochondrial morphological and functional changes as well as increased inflammation and cellular stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Soraya Savoj, Mohammad Hossein Nasr Esfahani, Akbar Karimi, Fereshteh Karamali
Summary: This study assessed the inducing activity of SCAP in the differentiation of hESCs into retinal cells in a 3D organoid system. Increasing the number of SCAPs improved the formation of the optic vesicle structure.
Article
Cell Biology
Guan-Yu Zhang, Zhu-Man Lv, Hao-Xin Ma, Yu Chen, Yuan Yuan, Ping-Xin Sun, Yu-Qi Feng, Ya-Wen Li, Wen-Jie Lu, Yu-Dong Yang, Cheng Yang, Xin-Lu Yu, Chao Wang, Shu-Long Liang, Ming-Liang Zhang, Hui-Liang Li, Wen-Lin Li
Summary: This study has developed an efficient method to induce pMN progenitors from human embryonic stem cells. These pMN progenitors can be stably maintained for multiple passages and retain their ability to rapidly produce spinal motor neurons and oligodendrocytes. These self-renewing pMN progenitors may have important clinical implications for the treatment of spinal cord injury and demyelinating disorders.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jieun Lee, Hal Sternberg, Paola A. Bignone, James Murai, Nafees N. Malik, Michael D. West, Dana Larocca
Summary: This study presents a panel of clonal embryonic endothelial progenitor cell lines derived from human embryonic stem cells. These cell lines exhibit characteristics of endothelial cells and show strong transcriptomic similarity to adult endothelial primary cell lines. The cells have demonstrated scalability and stable angiogenic properties, making them a potential source for therapeutic cells in cardiovascular disease treatment.
Article
Biochemistry & Molecular Biology
Seon-Ung Hwang, Kiyoung Eun, Mirae Kim, Junchul David Yoon, Lian Cai, Hyerin Choi, Dongjin Oh, Gabsang Lee, Hyunggee Kim, Eunhye Kim, Sang-Hwan Hyun
Summary: This study demonstrates successful establishment of neuro-organoids derived from porcine embryonic stem-like cells in vitro. The protocol developed in this study can serve as a tool for drug development, patient-specific chemotherapy, and human central nervous system disease studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Salomeh Salari, Eli Y. Adashi, Laura Keller, Timothy R. B. Johnson, Gary D. Smith
Summary: Human embryonic stem cells (hESCs) have shown great potential in disease modeling, understanding early human development, pharmaceutical screening, disease treatment, and regenerative medicine. This article discusses the current regulatory environment in the US and presents anonymized embryo donor statements to personalize the decision-making process for hESC derivation. The use of preimplantation genetic-tested and disease-specific hESCs provides valuable insights into early human genetics and developmental biology. Future applications of hESCs and other pluripotent stem cells in reproductive medicine are also discussed.
FERTILITY AND STERILITY
(2023)
