期刊
JOURNAL OF NEUROSCIENCE
卷 31, 期 41, 页码 14481-14487出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2950-11.2011
关键词
-
资金
- NIH [R21-NS045880, R01-NS041596, K99-NR010797, R21-NS060098, P20-RR15588]
- Christopher and Dana Reeve Foundation [TB2-0602]
Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for this in vivo. We have used a genetic approach to test for in vivo axonal transport of reporter mRNAs. We show that beta-actin's 3'-UTR can drive axonal localization of GFP mRNA in mature DRG neurons, but mice with gamma-actin's 3'-UTR show no axonal GFP mRNA. Peripheral axotomy triggers transport of the beta-actin 3'-UTR containing transgene mRNA into axons. This GFP-3'-beta-actin mRNA accumulates in injured PNS axons before activation of the transgene promoter peaks in the DRG. Spinal cord injury also increases axonal GFP signals in mice carrying this transgene without any increase in transgene expression in the DRGs. These data show for the first time that the beta-actin 3'-UTR is sufficient for axonal localization in both PNS and CNS neurons in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据