Article
Biology
Nandish Khanra, Patricia M. G. E. Brown, Amanda M. Perozzo, Derek Bowie, Joel R. Meyerson
Summary: The study presents the first structure of a KAR heteromer, showing how GluK2 subunits undergo structural rearrangements during desensitization to facilitate channel closure. It also reveals that the large conformational differences between antagonist-bound and desensitized states are mediated by the linkers connecting the pore helices to the ligand binding domains.
Review
Cell Biology
Satyendra Chandra Tripathi, Disha Vedpathak, Edwin Justin Ostrin
Summary: Cell-mediated immunity is driven by antigenic peptide presentation on major histocompatibility complex (MHC) molecules. Immunoproteasomes, with inducible activity by inflammatory stimuli, play central roles in cancer, autoimmunity, and inflammation by altering the catalytic activity of the proteasome.Understanding the roles of immunoproteasome in different cancers offers insights into developing targeted therapies.
Review
Biochemistry & Molecular Biology
Nishadh Rathod, Jessi J. Bak, Joseph O. Primeau, M'Lynn E. Fisher, Lennane Michel Espinoza-Fonseca, Mary Joanne Lemieux, Howard S. Young
Summary: SERCA is responsible for maintaining calcium homeostasis in cells and is regulated by the regulin family, including PLN and SLN, which inhibit its calcium transport properties. New members of the regulin family, such as MLN, ELN, and ALN, have been discovered and shown to have distinct inhibitory effects on SERCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ruijuan Guo, Huili Li, Rong Shi, Yun Wang
Summary: In this study, the synaptic delivery of spinal KA receptor subunits and the interaction between KA receptor subunits and GRIP were investigated during postoperative pain. The results showed that disrupting the GluK2- GRIP interaction could provide a new approach for relieving postoperative pain by reducing pain sensitivity and synaptic abundance of GluK2 in dorsal horn neurons.
NEUROCHEMICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Hanyu Zhang, Min Yang, Xu Wu, Qianxiu Li, Xin Li, Yueshui Zhao, Fukuan Du, Yu Chen, Zhigui Wu, Zhangang Xiao, Jing Shen, Qinglian Wen, Wei Hu, Chi Hin Cho, Meijuan Chen, Yejiang Zhou, Mingxing Li
Summary: Stromal cells in the gastric tumor microenvironment play a supportive role in tumor growth, while exosomes serve as crucial transporters of substances for cell-cell communication during tumor-stroma crosstalk. Therefore, exosomes present new biomarkers for gastric cancer diagnosis, prognosis, and potential treatment approaches.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Microbiology
Norihiro Takekawa, Akihiro Kawamoto, Mayuko Sakuma, Takayuki Kato, Seiji Kojima, Miki Kinoshita, Tohru Minamino, Keiichi Namba, Michio Homma, Katsumi Imade
Summary: The bacterial flagellum is a protein nanomachine that is essential for bacterial motility, with the MS-ring and M-ring in the earliest stages of assembly showing different rotational symmetries. The protein FliF adopts two distinct conformations in the M-ring, forming various symmetries for its multiple functions within the MS-ring.
Editorial Material
Immunology
Cindy Audiger, Sara Tomei, Shalin H. H. Naik
Summary: There is confusion regarding whether transitional dendritic cells are a distinct subset or simply a transitional state. Recent data have revealed heterogeneity within these cells, further complicating the matter.
Article
Biochemistry & Molecular Biology
Zhifeng Ye, Tingting Zhao, Qianhao Wei, Haoran Lin, Yong Zhang, Shuisheng Li
Summary: This study investigated the expression of vitellogenin and estrogen receptors in the liver of orange-spotted grouper. The results showed that their expression levels increased significantly during the breeding season and were upregulated by estradiol (E-2) injection. ER beta 2 was found to mediate the E-2-induced expression of vitellogenin and ER alpha, and the transcriptional activity of ER alpha was enhanced through a classical ERE-dependent pathway. This study provides insights into the roles of ER subtypes in VTG expression and the efficient VTG production in orange-spotted grouper during the breeding season.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mackenzie J. Thompson, Jaimee A. Domville, Claire H. Edrington, Angelica Venes, Patrick M. Giguere, John E. Baenziger
Summary: The outermost lipid-exposed alpha-helix (M4) in each of the homologous alpha, beta, delta, and gamma/epsilon subunits of the muscle nicotinic acetylcholine receptor (nAChR) is proposed as a lipid sensor. However, the mechanism of this sensor is not clear. To explore the function of M4 alpha-helix in each subunit of the nAChR, alanine mutations were characterized in alpha M4, beta M4, delta M4, and epsilon M4. The results suggest different functional roles for M4 alpha-helix in each subunit and lipid sensing occurs through cumulative interactions at the M4-M1/M3 interface.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Akihiro Ezoe, Kazumasa Shirai, Kousuke Hanada
Summary: Gene duplication is a major mechanism for creating new genes in plants, resulting in duplicated genes with varying degrees of functional diversification. High diversified duplicates from tandem duplications tend to have lineage-specific functions, while low diversified duplicates from whole-genome duplications are related to essential signaling pathways, impacting plant evolution differently.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Review
Neurosciences
Samantha C. Salvage, Kamalan Jeevaratnam, Christopher L. -H. Huang, Antony P. Jackson
Summary: The paragraph discusses the role of the beta 1 and beta 3 subunits in regulating the Na(V)1.5 sodium channel in cardiac myocytes, and how specific mutations in these subunits are associated with inherited cardiac arrhythmias. It also highlights new structural insights that raise questions about the physiological function of these proteins.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Multidisciplinary Sciences
Qiang Su, Mengying Chen, Yan Wang, Bin Li, Dan Jing, Xiechao Zhan, Yong Yu, Yigong Shi
Summary: This study investigates the structure of the murine PKD1L3/PKD2L1 heteromeric complex and the mechanism of calcium-induced channel activation, revealing a potential new Ca2+ binding site.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Nataliya V. Mushenkova, Nikita G. Nikiforov, Alexandra A. Melnichenko, Vladislav Kalmykov, Nikolay K. Shakhpazyan, Varvara A. Orekhova, Alexander N. Orekhov
Summary: This review discusses the functional phenotypes of intraplaque macrophages and their distinct contribution to atherosclerosis inflammation.
Review
Microbiology
Tohru Minamino, Miki Kinoshita, Keiichi Namba
Summary: Most motile bacteria use the flagellar type III secretion system (fT3SS) to construct the flagellum. The fT3SS consists of the transmembrane export complex and the cytoplasmic ATPase ring complex, which requires the FliH(12)-FliI(6)-FliJ(1) ring complex as an active protein transporter. The FliI(6) ring hydrolyzes ATP and FliJ activates the export complex. FliH anchors the FliI(6)-FliJ(1) ring to the base of the flagellum. The flagellar cytoplasmic ATPase complex plays multiple roles in efficient flagellar assembly.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Robert S. Oakes, Lisa H. Tostanoski, Senta M. Kapnick, Eugene Froimchuk, Sheneil K. Black, Xiangbin Zeng, Christopher M. Jewell
Summary: This study utilized nanotechnology to create immune complexes containing self-peptides and regulatory nucleic acids, which successfully reversed established paralysis in a preclinical MS model. Through a therapeutic library isolating specific components, it was found that regulatory nucleic acids suppress inflammatory genes, while disease-matched peptide sequences control specificity of tolerance.Distinct gene expression profiles were associated with the immune signals administered in particulate and soluble forms, indicating the importance of biophysical presentation of signals in driving tolerance.
Article
Endocrinology & Metabolism
Claudia A. Grillo, Gerardo G. Piroli, Robert C. Lawrence, Shayna A. Wrighten, Adrienne J. Green, Steven P. Wilson, Randall R. Sakai, Sandra J. Kelly, Marlene A. Wilson, David D. Mott, Lawrence P. Reagan
Article
Neurosciences
Jay F. Muller, Franco Mascagni, Violeta Zaric, David D. Mott, Alexander J. McDonald
JOURNAL OF COMPARATIVE NEUROLOGY
(2016)
Review
Neurosciences
Alexander J. McDonald, David D. Mott
JOURNAL OF NEUROSCIENCE RESEARCH
(2017)
Article
Neurosciences
Ana Fajardo-Serrano, Lei Liu, David D. Mott, Alexander J. McDonald
Article
Clinical Neurology
Denise K. Grosenbaugh, David D. Mott
Letter
Behavioral Sciences
David D. Mott, Denise K. Grosenbaugh, Janet L. Fisher
EPILEPSY & BEHAVIOR
(2013)
Article
Neurosciences
Janet L. Fisher, David D. Mott
JOURNAL OF NEUROSCIENCE
(2012)
Article
Neurosciences
Janet L. Fisher, David D. Mott
JOURNAL OF PHYSIOLOGY-LONDON
(2013)
Article
Geriatrics & Gerontology
Emily M. Stanley, Jim R. Fadel, David D. Mott
NEUROBIOLOGY OF AGING
(2012)
Article
Neurosciences
Mikhail Alexeev, Denise K. Grosenbaugh, David D. Mott, Janet L. Fisher
Article
Neurosciences
Denise K. Grosenbaugh, David D. Mott
Article
Psychology, Biological
Claudia A. Grillo, Gerardo G. Piroli, Ashlie N. Evans, Victoria A. Macht, Steven P. Wilson, Karen A. Scott, Randall R. Sakai, David D. Mott, Lawrence P. Reagan
PHYSIOLOGY & BEHAVIOR
(2011)
Article
Neurosciences
Alexander J. McDonald, Grace C. Jones, David D. Mott
Article
Neurosciences
Sarah C. Tryon, Joshua X. Bratsch-Prince, James W. Warren, Grace C. Jones, Alexander J. McDonald, David D. Mott
Summary: The amygdalar anterior basolateral nucleus (BLa) plays a crucial role in emotional behaviors, receiving dense cholinergic projections from the basal forebrain and modulating glutamatergic inputs. Electrophysiology and optogenetics experiments in mouse brain slices reveal that cholinergic signaling in BLa has a biphasic effect on cortical inputs, with rapid facilitation followed by slower depression. However, sustained elevation of acetylcholine only suppresses glutamatergic transmission at cortical inputs, not at midline thalamic nuclei inputs. The inhibition at cortex input requires presynaptic M4 mAChRs, while at thalamic input it depends on M3 mAChR-mediated endocannabinoid signaling. The inhibition at both pathways is frequency-dependent, allowing only high-frequency activity to pass.
JOURNAL OF NEUROSCIENCE
(2023)