Article
Developmental Biology
Cody J. Drozd, Christopher C. Quinn
Summary: This study reveals the role of UNC-116/KIF5C and NEKL-3/NEK6/7 kinase in axon targeting in Caenorhabditis elegans. UNC-116 and UNC-16/JIP3 play important roles in axon termination, and NEKL-3 functions with RPM-1 ubiquitin ligase to promote axon termination.
Article
Cell Biology
Hyun Gwan Park, Yeongjin David Kim, Eunsang Cho, Ting-Yi Lu, Chi-Kuang Yao, Jihye Lee, Seungbok Lee
Summary: Modulation of presynaptic actin dynamics is crucial for synaptic growth and functional plasticity. The Rho-type GEF Vav inhibits synaptic growth through macropinocytosis and plays a role in synaptic plasticity. The Vav-Rac1-SCAR signaling pathway independently regulates structural and functional presynaptic plasticity by driving macropinocytosis and mobilization of reserve pool vesicles.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Developmental Biology
Megan M. Corty, Alexandria L. Hulegaard, Jo Q. Hill, Amy E. Sheehan, Sue A. Aicher, Marc R. Freeman
Summary: This study uses Drosophila wrapping glia to investigate the development and function of non-myelinating axon ensheathment. The selective ablation of these glia severely impairs larval locomotor behavior. Through an RNA interference screen, the conserved receptor tyrosine kinase Discoidin domain receptor (Ddr) is identified as a key regulator in axon ensheathment. Loss of Ddr results in reduced ensheathment of axons and decreased axon caliber, suggesting that Ddr functions as a collagen receptor to drive axon wrapping. In adult nerves, loss of Ddr decreases long-term survival of sensory neurons and significantly reduces axon caliber without overtly affecting ensheathment.
Article
Physiology
Caroline Heymann, Christine Paul, Na Huang, Jaqueline C. C. Kinold, Ann-Christin Dietrich, Hermann Aberle
Summary: This study presents a structure-function analysis of the guidance molecules Sidestep (Side) and Beaten path Ia (Beat) in Drosophila. The results show that a single amino acid mutation in the second immunoglobulin domain of Side disrupts its function and subcellular localization. The first immunoglobulin domains of Side and Beat are found to be necessary for their interaction. Additionally, the subcellular distribution of Beat constructs suggests a potential posttranslational processing step in the endoplasmic reticulum (ER) compartments.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Liwei Zhang, Xuan Guo, Wei Zhang
Summary: This study reveals that sugar intake immediately suppresses the sexual drive of male Drosophila, a regulation that is mediated by insulin signaling and the action of P1 neurons. The same pathway is also utilized by anaphrodisiac pheromones to suppress sexual hyperactivity. Additionally, repellent pheromones induce the release of CCAP, which triggers insulin release and inhibits P1 neurons.
Article
Neurosciences
Kar Men Mah, Wei Wu, Hassan Al-Ali, Yan Sun, Qi Han, Ying Ding, Melissa Munoz, Xiao-Ming Xu, Vance P. Lemmon, John L. Bixby
Summary: The recovery from spinal cord injury and other central nervous system trauma is limited by the ability of axonal regeneration. By targeting kinases involved in both intrinsic and extrinsic regulatory pathways, a therapeutic strategy has been developed to promote axon growth and regeneration. This strategy has shown promising results in vitro as well as in multiple mouse models of spinal cord injury, suggesting its potential for clinical application.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Cell Biology
Pablo Guzman-Palma, Esteban G. Contreras, Natalia Mora, Macarena Smith, M. Constanza Gonzalez-Ramirez, Jorge M. Campusano, Jimena Sierralta, Bassem A. Hassan, Carlos Oliva
Summary: The Slit-Robo pathway is involved in the development of motion sensitive neurons in the Drosophila visual system, regulating processes such as neuronal precursor migration, cell segregation, and connectivity pattern formation. This signaling pathway plays a versatile role in different developmental stages of a neural lineage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Mengying Yang, Yige Guo, Shuran Wang, Changyan Chen, Yung-Heng Chang, Margaret Su-chun Ho
Summary: Protein homeostasis plays a crucial role in regulating cellular processes in the nervous system. The ubiquitination proteasome system (UPS) is involved in the development of synaptic structures, such as the Drosophila mushroom body, and the newly characterized Drosophila F-box protein CG5003 contributes to MB development. Depletion of CG5003 leads to axon defects in MB neurons, highlighting its role in regulating neuronal development.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Oncology
Jana Koch, Dina Moench, Annika Maass, Alina Mangold, Miodrag Guzvic, Thomas Muerdter, Tobias Leibold, Marc-H Dahlke, Philipp Renner
Summary: We investigated the effects of MMPs on the peritoneal attachment of colorectal cancer cells. Overexpression of MMP2/9 and enhanced cleavage of fibronectin occurred during peritoneal colonization, which could be inhibited by specific MMP inhibition, thereby reducing cancer cell attachment.
Article
Multidisciplinary Sciences
Tomonori Shibata, Konami Nagano, Morio Ueyama, Kensuke Ninomiya, Tetsuro Hirose, Yoshitaka Nagai, Kinya Ishikawa, Gota Kawai, Kazuhiko Nakatani
Summary: The study identifies naphthyridine carbamate dimer (NCD) as a compound that targets disease-causing r(UGGAA)(n) repeat RNAs in spinocerebellar ataxia type 31. NCD disrupts naturally occurring RNA foci built on repeat RNA and suppresses splicing events by interfering with RNA-protein interactions.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Chi-Chiu Lee, Chia-Jui Yu, Sushree Shankar Panda, Kai-Chi Chen, Kang-Hao Liang, Wan-Chen Huang, Yu-Shiuan Wang, Pei-Chin Ho, Han-Chung Wu
Summary: This study explores the interaction between EpCAM and HGFR and its impact on the metastatic and invasive abilities of colon cancer cells. The results demonstrate that EpCAM binds to HGFR and activates the ERK and FAK-AKT signaling pathways, leading to the promotion of colon cancer metastasis. Furthermore, the combined treatment with EpCAM and HGFR antibodies significantly inhibits tumor progression and improves patient survival.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Qingyang Li, Chao Zhang, Chuchu Zhang, Renjie Duan, Yongzhi Hua
Summary: In this study, the immune function of CG4968, which encodes a typical OTU-type Dub in Drosophila melanogaster, was explored. The results showed that CG4968 plays a positive regulatory role in the IMD pathway, but not the Toll pathway, in a manner dependent on the OTU domain. Mechanistically, CG4968 was found to be associated with Imd and restrict its K48-linked ubiquitination, thereby contributing to its turnover. This study uncovered a novel regulatory mechanism involving the K48-linked ubiquitination of Imd in Drosophila innate immunity.
Article
Multidisciplinary Sciences
Elena E. Grintsevich, Giasuddin Ahmed, Anush A. Ginosyan, Heng Wu, Shannon K. Rich, Emil Reisler, Jonathan R. Terman
Summary: Cellular events require the spatiotemporal interplay between actin assembly and disassembly. Profilin and Mical combine to impair F-actin assembly and promote F-actin disassembly, while simultaneously facilitating cellular remodeling and plasticity.Actin-based structures in cells and tissues are built and maintained through a poorly understood balance between assembly and disassembly.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Michael Hobin, Katherine Dorfman, Mohamed Adel, Emmanuel J. Rivera-Rodriguez, Elena A. Kuklin, Dingbang Ma, Leslie C. Griffith
Summary: The sleep-regulating microRNA bantam in adult Drosophila is found to promote early nighttime sleep through inhibiting a population of glutamatergic neurons, which play a role in contextual information processing. This study identifies a new role for bantam as an active regulator of sleep and neural activity.
Article
Cardiac & Cardiovascular Systems
Li Liu, Qianwen Zhao, Ming Kong, Lei Mao, Yuyu Yang, Yong Xu
Summary: Macrophage-specific deletion of MRTF-A can alleviate cardiac hypertrophy, reduce heart function loss, and decrease cardiac fibrosis. The study suggests that MRTF-A may regulate macrophage activity and contribute to the pathogenesis of cardiac hypertrophy by activating ITGB2 transcription.
CARDIOVASCULAR RESEARCH
(2022)
Article
Developmental Biology
James C. Sears, Heather T. Broihier
DEVELOPMENTAL BIOLOGY
(2016)
Article
Cell Biology
Colleen N. McLaughlin, Inna V. Nechipurenko, Nan Liu, Heather T. Broihier
JOURNAL OF CELL BIOLOGY
(2016)
Editorial Material
Biochemistry & Molecular Biology
Heather T. Broihier
Editorial Material
Biochemistry & Molecular Biology
Heather T. Broihier
Article
Neurosciences
Kelsey A. Herrmann, Heather T. Broihier
CURRENT OPINION IN NEUROBIOLOGY
(2018)
Review
Genetics & Heredity
Colleen N. McLaughlin, Heather T. Broihier
TRENDS IN GENETICS
(2018)
Article
Developmental Biology
Rebecca E. James, Heather T. Broihier
Article
Cell Biology
Rebecca E. James, Kendall M. Hoover, Dinara Bulgari, Colleen N. McLaughlin, Christopher G. Wilson, Kristi A. Wharton, Edwin S. Levitan, Heather T. Broihier
DEVELOPMENTAL CELL
(2014)
Article
Cell Biology
Inna V. Nechipurenko, Heather T. Broihier
JOURNAL OF CELL BIOLOGY
(2012)
Article
Neurosciences
Yi-Lan Weng, Nan Liu, Aaron DiAntonio, Heather T. Broihier
JOURNAL OF NEUROSCIENCE
(2011)
Article
Cell Biology
Colleen N. McLaughlin, Jahci J. Perry-Richardson, Jaeda C. Coutinho-Budd, Heather T. Broihier
DEVELOPMENTAL CELL
(2019)
Editorial Material
Cell Biology
Heather T. Broihier
JOURNAL OF CELL BIOLOGY
(2019)
Article
Genetics & Heredity
Kelsey A. A. Herrmann, Yizhou Liu, Arnau Llobet-Rosell, Colleen N. N. McLaughlin, Lukas J. J. Neukomm, Jaeda C. C. Coutinho-Budd, Heather T. T. Broihier
Summary: This study reveals divergent signaling requirements for dSARM in glial phagocytosis and axon degeneration. The results demonstrate that the NAD(+) hydrolase activity of dSARM is essential for both functions, while SAM domain-mediated multimerization is only important for axon degeneration. In addition, dSARM functions in a genetic pathway with MAP3K Ask1 during development.