Article
Cell Biology
Kun-Zhang Lin, Lei Li, Wen-Yu Ma, Xin Yang, Zeng-Peng Han, Neng-Song Luo, Jie Wang, Fu-Qiang Xu
Summary: Analyzing and understanding the brain's neural network is crucial for unraveling brain functioning and identifying brain diseases. Recombinant rabies viral vectors offer a powerful tool for studying synaptic inputs, but their use is limited by long preparation cycles and low yield. To overcome these limitations, a new production system for the CVS-N2c-Delta G virus was developed, demonstrating enhanced efficiency in accessing projection neurons and trans-monosynaptic tracing. The CVS-N2c-Delta G-based toolkit proved versatile in neural circuit studies, showcasing potential for structural and functional investigations.
NEURAL REGENERATION RESEARCH
(2023)
Article
Behavioral Sciences
Ulrich Stefan Bauer, Vegard Fiskum, Rajeevkumar Raveendran Nair, Rosanne van de Wijdeven, Clifford Kentros, Ioanna Sandvig, Axel Sandvig
Summary: In vitro neuroengineering approaches offer significant benefits for studying and manipulating neurons, networks, and functional units selectively in preclinical models of neurodegenerative diseases like ALS. Custom-designed compartmentalized microfluidic culture systems facilitate co-culturing of different cell types in interconnected microenvironments, enabling the study of neuromuscular junctions and mechanistic aspects of ALS pathological cascade. The functionality of engineered neuromuscular junctions can be demonstrated within a microfluidic chip using designer pseudotyped & UDelta;G-rabies virus for retrograde monosynaptic tracing.
FRONTIERS IN INTEGRATIVE NEUROSCIENCE
(2022)
Article
Neurosciences
Shenqin Yao, Quanxin Wang, Karla E. Hirokawa, Benjamin Ouellette, Ruweida Ahmed, Jasmin Bomben, Krissy Brouner, Linzy Casal, Shiella Caldejon, Andy Cho, Nadezhda I. Dotson, Tanya L. Daigle, Tom Egdorf, Rachel Enstrom, Amanda Gary, Emily Gelfand, Melissa Gorham, Fiona Griffin, Hong Gu, Nicole Hancock, Robert Howard, Leonard Kuan, Sophie Lambert, Eric Kenji Lee, Jennifer Luviano, Kyla Mace, Michelle Maxwell, Marty T. Mortrud, Maitham Naeemi, Chelsea Nayan, Nhan-Kiet Ngo, Thuyanh Nguyen, Kat North, Shea Ransford, Augustin Ruiz, Sam Seid, Jackie Swapp, Michael J. Taormina, Wayne Wakeman, Thomas Zhou, Philip R. Nicovich, Ali Williford, Lydia Potekhina, Medea McGraw, Lydia Ng, Peter A. Groblewski, Bosiljka Tasic, Stefan Mihalas, Julie A. Harris, Ali Cetin, Hongkui Zeng
Summary: Identification of structural connections between neurons is essential for understanding brain function. In this study, the researchers developed a systematic mapping process to determine brain-wide monosynaptic input connections in genetically defined neuronal populations and discovered differences in target specificity, layer specificity, and cell class specificity in the visual cortex.
NATURE NEUROSCIENCE
(2023)
Article
Neurosciences
Wei-Xiang Ma, Lei Li, Ling-Xi Kong, Hui Zhang, Ping-Chuan Yuan, Zhi-Li Huang, Yi-Qun Wang
Summary: This study investigates the monosynaptic inputs to glutamatergic neurons in the lateral periaqueductal gray (LPAG), which is involved in social responses, pain, and offensive and defensive behaviors. Using retrograde tracing systems and immunofluorescence analysis, the study reveals that 59 nuclei project monosynaptic inputs to LPAG glutamatergic neurons, with the hypothalamus being the most densely connected. Furthermore, the input neurons are colocalized with markers related to physiological behaviors.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biology
Maribel Patino, Willian N. Lagos, Neelakshi S. Patne, Paula A. Miyazaki, Sai Krishna Bhamidipati, Forrest Collman, Edward M. Callaway
Summary: Retrograde monosynaptic tracing is an important tool for studying neural circuit structure and connectivity. This study used a glycoprotein-deleted rabies virus to label synaptic contacts in the mouse primary visual cortex and found similar proportions of labeled contacts across different cell types and subcellular locations.
Article
Microbiology
Caiquan Zhao, Jie Gao, Yongzhi Wang, Lina Ji, Hui Qin, Wei Hu, Yang Yang
Summary: This study describes a novel recombinant bovine herpes virus type I expressing rabies virus glycoprotein, which induced a protective immune response in mice and cattle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Feng Xiong, Hong Yang, Yi-Ge Song, Hai-Bin Qin, Qing-Yang Zhang, Xian Huang, Wei Jing, Manfei Deng, Yang Liu, Zhixiang Liu, Yin Shen, Yunyun Han, Youming Lu, Xiangmin Xu, Todd C. Holmes, Minmin Luo, Fei Zhao, Min-Hua Luo, Wen-Bo Zeng
Summary: The H129(Amp) tracer system is a powerful tool for tracing neuronal connectomes. It allows for fast and bright monosynaptic tracing with low toxicity through the use of an HSV amplicon-based system.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Yuji Masaki, Masahiro Yamaguchi, Ryosuke F. Takeuchi, Fumitaka Osakada
Summary: The study established an efficient RVAG tracing method to label the input-output organization of single neuronal networks, aiding in analyzing the heterogeneity of neural circuits and revealing information processing and circuit computation in the brain.
NEUROSCIENCE RESEARCH
(2022)
Article
Neurosciences
Zengpeng Han, Nengsong Luo, Jiaxin Kou, Lei Li, Zihong Xu, Siyuan Wei, Yang Wu, Jie Wang, Chaohui Ye, Kunzhang Lin, Fuqiang Xu
Summary: Retrograde tracers based on viral vectors are important tools for studying neural circuits. However, current tracers have limitations. We have developed a new method and a cell-type-specific retrograde tracing system to overcome these limitations.
Article
Virology
Meina Cai, Ziliang Hu, Yi Yang, Ting Mao, Yacui Liu, Guangwen Lu, Fanli Yang, Jianxun Qi, Weijin Huang, Youchun Wang
Summary: Currently, the cost and limited availability of horse or human rabies immunoglobulin (RIG) for postexposure prevention of human rabies (PEP) need to be addressed. Mouse and human monoclonal antibodies have shown promise in protecting against lethal rabies virus (RABV) attacks. In this study, a human-mouse chimeric monoclonal antibody, 12-2A12, demonstrated strong neutralization potency against multiple street viruses of RABV. The antibody targets the viral glycoprotein (G) and may interfere with the receptor binding and membrane fusion that are crucial for viral infection.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Neurosciences
Hong-Yan Geng, Gordon Arbuthnott, Wing-Ho Yung, Ya Ke
Summary: This study mapped all long-range monosynaptic inputs to M1 deep output neurons in layer 5 (L5) and revealed that most upstream areas innervate both dendritic compartments concurrently. The dichotomous inputs arise mostly from spatially segregated neuronal subpopulations within an upstream nucleus, indicating a previously unknown and highly intricate synaptic input pattern of M1L5 neurons.
Article
Infectious Diseases
Tong Xu, Lele Liu, Chenjuan Shi, Wenkai Liu, Min Wang, Li Tian, Ye Zheng, Hongmei Wang, Wenwen Zheng, Hongbin He, Xianzhu Xia, Xuexing Zheng
Summary: A bivalent vaccine targeting cystic echinococcosis (CE) and rabies, generated in this study, showed promising results in protecting sheep and cattle in endemic areas against both diseases. This vaccine induced robust immune responses and provided high levels of protection in animal models.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Multidisciplinary Sciences
Nikolas L. Jorstad, Janet H. T. Song, David Exposito-Alonso, Hamsini Suresh, Nathan Castro-Pacheco, Fenna M. Krienen, Anna Marie Yanny, Jennie Close, Emily Gelfand, Brian Long, Stephanie C. Seeman, Kyle J. Travaglini, Soumyadeep Basu, Marc Beaudin, Darren Bertagnolli, Megan Crow, Song-Lin Ding, Jeroen Eggermont, Alexandra Glandon, Jeff Goldy, Katelyn Kiick, Thomas Kroes, Delissa Mcmillen, Trangthanh Pham, Christine Rimorin, Kimberly Siletti, Saroja Somasundaram, Michael Tieu, Amy Torkelson, Guoping Feng, William D. Hopkins, Thomas Hollt, C. Dirk Keene, Sten Linnarsson, Steven A. Mccarroll, Boudewijn P. Lelieveldt, Chet C. Sherwood, Kimberly Smith, Christopher A. Walsh, Alexander Dobin, Jesse Gillis, Ed S. Lein, Rebecca D. Hodge, Trygve E. Bakken
Summary: Our study used snRNA-seq to analyze the transcriptome of the cerebral cortex in humans and four nonhuman primate species. We found that cell types in the cerebral cortex are highly conserved among great apes, with marmosets showing some unique cell type proportions. Profiling gorillas allowed us to identify expression differences between humans and chimpanzees that are specialized in humans. We also discovered that neuronal gene expression has evolved faster on the human lineage, while glial expression changes have occurred rapidly in all species. Human-specific DEGs are enriched near adaptive genomic changes and may contribute to specialized cortical function.
Article
Environmental Sciences
Zhang Jun Nan, Meng Ya Juan, Bai Yun Hua, Li Yu Feng, Yang Li Qing, Shi Nian Min, Han Hui Xia, Gao Jian, Zhu Li Juan, Li Shu Ping, Zhang Jing, Zhao Qin Hua, Wang Xiu Qin, Wei Jing Shuang, Ren Le Min, Cao Chen Hua, Chen Chen, Zhao Wei, Li Li
Summary: This study aimed to evaluate the rabies virus neutralizing activity, safety, and immunogenicity of a recombinant human rabies antibody (NM57) compared with human rabies immunoglobulin (HRIG) in Chinese healthy adults. The results showed that the neutralizing activity of 40 IU/kg NM57 was superior to that of 20 IU/kg NM57 and 20 IU/kg HRIG, while the neutralizing activity of 20 IU/kg NM57 and 20 IU/kg HRIG were similar. Safety was comparable between NM57 and HRIG.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Sabrina Kali, Corinne Jallet, Saliha Azebi, Thomas Cokelaer, Juliana Pipoli Da Fonseca, Yu Wu, Julien Barbier, Jean-Christophe Cintrat, Daniel Gillet, Noel Tordo
Summary: ABMA and DABMA, members of the adamantane family, have been shown to inhibit cell infection of the Rabies Virus in vitro by perturbing the endosomal pathway. They act at different stages of the late endosomal pathway and exhibit different profiles of synergy/antagonism with other antiviral drugs. These results suggest new therapeutic perspectives against rabies.
ANTIVIRAL RESEARCH
(2021)
Article
Neurosciences
Euiseok J. Kim, Zhuzhu Zhang, Ling Huang, Tony Ito-Cole, Matthew W. Jacobs, Ashley L. Juavinett, Gokhan Senturk, Mo Hu, Manching Ku, Joseph R. Ecker, Edward M. Callaway
Article
Biology
Shinya Ohara, Stefan Blankvoort, Rajeevkumar Raveendran Nair, Maximiliano J. Nigro, Eirik S. Nilssen, Clifford Kentros, Menno P. Witter
Summary: Research indicates the presence of different operational principles in the connections from layer Vb to Va in the entorhinal cortex, with these connections being stronger in dorsal LEC compared to dorsal MEC. This suggests a potential difference in how LEC and MEC mediate episodic memory consolidation in the brain.
Article
Neurosciences
Regina L. Faulkner, Nicholas R. Wall, Edward M. Callaway, Hollis T. Cline
Summary: The Xenopus laevis experimental system has provided important insights into the development and plasticity of neural circuits, and additional tools are needed to study the structure and function of neural circuits. Rabies virus has been widely used to study mesoscale connectomics but its use in transducing neurons and expressing transgenes in Xenopus has not been systematically investigated.
Article
Biochemistry & Molecular Biology
Megan A. Kirchgessner, Alexis D. Franklin, Edward M. Callaway
Summary: The study demonstrates different functional influences of layer 5 and layer 6 corticothalamic pathways on the higher-order thalamus (HO), with layer 5 projections driving visual responses in the pulvinar. Additionally, it shows that inputs from cortical layer 5 and subcortical structures both contribute to visual thalamus activity in awake mice.
Article
Multidisciplinary Sciences
Edward M. Callaway, Hong-Wei Dong, Joseph R. Ecker, Michael J. Hawrylycz, Z. Josh Huang, Ed S. Lein, John Ngai, Pavel Osten, Bing Ren, Andreas Savas Tolias, Owen White, Hongkui Zeng, Xiaowei Zhuang, Giorgio A. Ascoli, M. Margarita Behrens, Jerold Chun, Guoping Feng, James C. Gee, Satrajit S. Ghosh, Yaroslav O. Halchenko, Ronna Hertzano, Byung Kook Lim, Maryann E. Martone, Lydia Ng, Lior Pachter, Alexander J. Ropelewski, Timothy L. Tickle, X. William Yang, Kun Zhang, Trygve E. Bakken, Philipp Berens, Tanya L. Daigle, Julie A. Harris, Nikolas L. Jorstad, Brian E. Kalmbach, Dmitry Kobak, Yang Eric Li, Hanqing Liu, Katherine S. Matho, Eran A. Mukamel, Maitham Naeemi, Federico Scala, Pengcheng Tan, Jonathan T. Ting, Fangming Xie, Meng Zhang, Zhuzhu Zhang, Jingtian Zhou, Brian Zingg, Darren Bertagnolli, Tamara Casper, Kirsten Crichton, Nick Dee, Dinh Diep, Song-Lin Ding, Weixiu Dong, Elizabeth L. Dougherty, Olivia Fong, Melissa Goldman, Jeff Goldy, Rebecca D. Hodge, Lijuan Hu, C. Dirk Keene, Fenna M. Krienen, Matthew Kroll, Blue B. Lake, Kanan Lathia, Sten Linnarsson, Christine S. Liu, Evan Z. Macosko, Steven A. McCarroll, Delissa McMillen, Naeem M. Nadaf, Thuc Nghi Nguyen, Carter R. Palmer, Thanh Pham, Nongluk Plongthongkum, Nora M. Reed, Aviv Regev, Christine Rimorin, William J. Romanow, Steven Savoia, Kimberly Siletti, Kimberly Smith, Josef Sulc, Bosiljka Tasic, Michael Tieu, Amy Torkelson, Herman Tung, Cindy T. J. van Velthoven, Charles R. Vanderburg, Anna Marie Yanny, Rongxin Fang, Xiaomeng Hou, Jacinta D. Lucero, Julia K. Osteen, Antonio Pinto-Duarte, Olivier Poirion, Sebastian Preissl, Xinxin Wang, Andrew Aldridge, Anna Bartlett, Lara Boggeman, Carolyn O'Connor, Rosa G. Castanon, Huaming Chen, Conor Fitzpatrick, Chongyuan Luo, Joseph R. Nery, Michael Nunn, Angeline C. Rivkin, Wei Tian, Bertha Dominguez, Tony Ito-Cole, Matthew Jacobs, Xin Jin, Cheng-Ta Lee, Kuo-Fen Lee, Paula Assakura Miyazaki, Yan Pang, Mohammad Rashid, Jared B. Smith, Minh Vu, Elora Williams, Ethan Armand, Tommaso Biancalani, A. Sina Booeshaghi, Megan Crow, Sandrine Dudoit, Stephan Fischer, Jesse Gillis, Qiwen Hu, Peter Kharchenko, Sheng-Yong Niu, Vasilis Ntranos, Elizabeth Purdom, Davide Risso, Hector Roux de Bezieux, Saroja Somasundaram, Kelly Street, Valentine Svensson, Eeshit Dhaval Vaishnav, Koen Van den Berge, Joshua D. Welch, Zizhen Yao, Xu An, Helen S. Bateup, Ian Bowman, Rebecca K. Chance, Nicholas N. Foster, William Galbavy, Hui Gong, Lin Gou, Joshua T. Hatfield, Houri Hintiryan, Karla E. Hirokawa, Gukhan Kim, Daniel J. Kramer, Anan Li, Xiangning Li, Qingming Luo, Rodrigo Munoz-Castaneda, David A. Stafford, Zhao Feng, Xueyan Jia, Shengdian Jiang, Tao Jiang, Xiuli Kuang, Rachael Larsen, Phil Lesnar, Yaoyao Li, Yuanyuan Li, Lijuan Liu, Hanchuan Peng, Lei Qu, Miao Ren, Zongcai Ruan, Elise Shen, Yuanyuan Song, Wayne Wakeman, Peng Wang, Yimin Wang, Yun Wang, Lulu Yin, Jing Yuan, Sujun Zhao, Xuan Zhao, Arun Narasimhan, Ramesh Palaniswamy, Samik Banerjee, Liya Ding, Dhananjay Huilgol, Bingxing Huo, Hsien-Chi Kuo, Sophie Laturnus, Xu Li, Partha P. Mitra, Judith Mizrachi, Quanxin Wang, Peng Xie, Feng Xiong, Yang Yu, Stephen W. Eichhorn, Jim Berg, Matteo Bernabucci, Yves Bernaerts, Cathryn Rene Cadwell, Jesus Ramon Castro, Rachel Dalley, Leonard Hartmanis, Gregory D. Horwitz, Xiaolong Jiang, Andrew L. Ko, Elanine Miranda, Shalaka Mulherkar, Philip R. Nicovich, Scott F. Owen, Rickard Sandberg, Staci A. Sorensen, Zheng Huan Tan, Shona Allen, Dirk Hockemeyer, Angus Y. Lee, Matthew B. Veldman, Ricky S. Adkins, Seth A. Ament, Hector Corrada Bravo, Robert Carter, Apaala Chatterjee, Carlo Colantuoni, Jonathan Crabtree, Heather Creasy, Victor Felix, Michelle Giglio, Brian R. Herb, Jayaram Kancherla, Anup Mahurkar, Carrie McCracken, Lance Nickel, Dustin Olley, Joshua Orvis, Michael Schor, Greg Hood, Benjamin Dichter, Michael Grauer, Brian Helba, Anita Bandrowski, Nikolaos Barkas, Benjamin Carlin, Florence D. D'Orazi, Kylee Degatano, Thomas H. Gillespie, Farzaneh Khajouei, Kishori Konwar, Carol Thompson, Kathleen Kelly, Stephanie Mok, Susan Sunkin
Summary: This study presents a multimodal cell census and atlas of the mammalian primary motor cortex, integrating various molecular and spatial information to reveal a unified genetic landscape of cortical cell types. The results establish a mechanistic framework of neuronal cell-type organization by linking molecular genetic information with phenotypic properties.
Article
Multidisciplinary Sciences
Hanqing Liu, Jingtian Zhou, Wei Tian, Chongyuan Luo, Anna Bartlett, Andrew Aldridge, Jacinta Lucero, Julia K. Osteen, Joseph R. Nery, Huaming Chen, Angeline Rivkin, Rosa G. Castanon, Ben Clock, Yang Eric Li, Xiaomeng Hou, Olivier B. Poirion, Sebastian Preissl, Antonio Pinto-Duarte, Carolyn O'Connor, Lara Boggeman, Conor Fitzpatrick, Michael Nunn, Eran A. Mukamel, Zhuzhu Zhang, Edward M. Callaway, Bing Ren, Jesse R. Dixon, M. Margarita Behrens, Joseph R. Ecker
Summary: The study comprehensively assessed the epigenomes of different cell types in the mouse brain, revealing the diversity and spatial organization of cell types, as well as the repetitive usage of regulators for distinguishing cell subtypes. By constructing an artificial neural network model, it is possible to accurately predict the cell type and spatial location of individual neurons in the brain.
Article
Multidisciplinary Sciences
Zhuzhu Zhang, Jingtian Zhou, Pengcheng Tan, Yan Pang, Angeline C. Rivkin, Megan A. Kirchgessner, Elora Williams, Cheng-Ta Lee, Hanqing Liu, Alexis D. Franklin, Paula Assakura Miyazaki, Anna Bartlett, Andrew Aldridge, Minh Vu, Lara Boggeman, Conor Fitzpatrick, Joseph R. Nery, Rosa G. Castanon, Mohammad Rashid, Matthew W. Jacobs, Tony Ito-Cole, Carolyn O'Connor, Antonio Pinto-Duartec, Bertha Dominguez, Jared B. Smith, Sheng-Yong Niu, Kuo-Fen Lee, Xin Jin, Eran A. Mukamel, M. Margarita Behrens, Joseph R. Ecker, Edward M. Callaway
Summary: By combining retrograde labeling with single-nucleus DNA methylation sequencing, researchers were able to identify unique epigenetic signatures of projection neurons that corresponded to their laminar and regional location and projection patterns. These findings helped to distinguish intra-telencephalic cells projecting to different cortical targets and confirmed the correlation between L5ET cortical neurons and their axonal projections.
Article
Neurosciences
Jonathan R. Cook, Hao Li, Bella Nguyen, Hsiang-Hsuan Huang, Payaam Mahdavian, Megan A. Kirchgessner, Patrick Strassmann, Max Engelhardt, Edward M. Callaway, Xin Jin
Summary: The study reveals a mechanism for action timing in mice mediated by the secondary auditory cortex, showing that auditory deprivation disrupts learned action timing. Neuronal activity in the secondary auditory cortex is both correlated with and necessary for proper execution of learned action timing.
NATURE NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Maribel Patino, Will N. Lagos, Neelakshi S. Patne, Bosiljka Tasic, Hongkui Zeng, Edward M. Callaway
Summary: Using modified rabies virus for cortical circuit tracing can identify input neurons directly connecting to neurons of interest. We used single-nucleus RNA sequencing to study the gene expression profiles of rabies-infected neurons and found that they can be characterized transcriptomically despite global and cell-type-specific transcriptional changes induced by rabies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ying Zhang, Dheeraj S. Roy, Yi Zhu, Yefei Chen, Tomomi Aida, Yuanyuan Hou, Chenjie Shen, Nicholas E. Lea, Margaret E. Schroeder, Keith M. Skaggs, Heather A. Sullivan, Kyle B. Fischer, Edward M. Callaway, Ian R. Wickersham, Ji Dai, Xiao-Ming Li, Zhonghua Lu, Guoping Feng
Summary: This study suggests that targeting the neural circuits in the parafascicular thalamus may be an effective strategy for treating both motor and non-motor symptoms in Parkinson's disease.
Article
Neurosciences
Helen Wang, Oyshi Dey, Willian N. Lagos, Edward M. Callaway
Summary: The mouse visual system consists of various cortical areas specialized for different visual features and tasks. Previous studies mainly focused on the tuning preferences of superficial layer neurons, while fewer studies investigated the deep-layer neurons in these areas. In this study, the researchers used electrophysiology and calcium imaging to characterize the tuning properties of different layer 5 cell classes in these areas. They found that deep-layer neurons, similar to superficial layer neurons, are specialized for different spatial and temporal frequencies.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Peichao Li, Anupam K. Garg, Li A. Zhang, Mohammad S. Rashid, Edward M. Callaway
Summary: This study demonstrates that cone-opponent signals are integrated through organized circuits in the primary visual cortex, leading to the orderly transition from cone-opponency to color appearance.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Kyle B. Fischer, Hannah K. Collins, Yan Pang, Dheeraj S. Roy, Ying Zhang, Guoping Feng, Shu-Jing Li, Adam Kepecs, Edward M. Callaway
Summary: Identification of synaptic partners is a fundamental task, but reliable techniques for whole brain labeling of downstream partners in a cell-type-dependent and monosynaptic manner are lacking. In this study, the authors developed a novel anterograde tracing system based on genetic manipulation of a virus, resulting in reliable labeling of postsynaptic partners in a brain-wide fashion. This system provides a valuable tool for studying neural circuits.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Neurosciences
Joachim S. Grimstvedt, Andrew M. Shelton, Anna Hoerder-Suabedissen, David K. Oliver, Christin H. Berndtsson, Stefan Blankvoort, Rajeevkumar R. Nair, Adam M. Packer, Menno P. Witter, Clifford G. Kentros
Summary: Accurate anatomical characterizations of the claustrum complex (CLCX) in rodents are important for studying its neural circuitry. This study utilized a multifaceted analysis to investigate the fiber- and cytoarchitecture, genetic marker expression, and connectivity of the CLCX in mice. The findings identified four distinct subregions within CLCX and provided a comprehensive guide for delineating its boundaries. This research contributes to enhancing our anatomical knowledge of the CLCX and its functional properties.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Biology
Maribel Patino, Willian N. Lagos, Neelakshi S. Patne, Paula A. Miyazaki, Sai Krishna Bhamidipati, Forrest Collman, Edward M. Callaway
Summary: Retrograde monosynaptic tracing is an important tool for studying neural circuit structure and connectivity. This study used a glycoprotein-deleted rabies virus to label synaptic contacts in the mouse primary visual cortex and found similar proportions of labeled contacts across different cell types and subcellular locations.