Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: This study examined the temporal and concentration effects of chemical stimulation on exocytotic molecular plasticity. The results showed that catecholamine release and release dynamics depended on the concentration of the stimulant solution. Increased release was observed at 30 and 50 mM stimulation, while decreased release was observed at 100 mM stimulation. These findings suggest a possible link between stimulation intensity and synaptic (or adrenal) plasticity.
Article
Neurosciences
Che-Wei Chang, Yu-Tien Hsiao, Meyer B. Jackson
Summary: This study demonstrates that Synaptophysin (Syp) and synaptogyrin (Syg) play a regulatory role in dense-core vesicle function at various stages, affecting fusion initiation, mode selection between full fusion and kiss-and-run, and influencing the dynamics of both initial and late-stage fusion pores. The transmembrane domain (TMD) of Syp influences small initial fusion pores, while the C-terminal domain influences large late-stage fusion pores, possibly through an interaction with dynamin.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Chemistry, Analytical
Nicolas Maino, Arnaud Bertsch, Philippe Renaud
Summary: For more than 30 years, carbon fiber microelectrodes have been the gold standard for measuring processes at the synaptic level. However, these microelectrodes have limitations in terms of low throughput and detecting molecules due to their featureless nature. In this study, a new electrochemical sensor with insulated protruding volcano-shaped tips is introduced, which allows confined and parallel recordings of exocytosis from adherent cells. The sensor reveals faster exocytosis kinetics, potentially due to the perturbation of the plasma membrane by the sensor's topography, suggesting that substrate geometry can manipulate exocytosis kinetics and open up new avenues for studying synaptic processes.
Article
Chemistry, Multidisciplinary
Zhaohan Lin, Yinglin Li, Yuqi Hang, Changhe Wang, Bing Liu, Jie Li, Lili Yin, Xiaohan Jiang, Xingyu Du, Zhongjun Qiao, Feipeng Zhu, Zhe Zhang, Quanfeng Zhang, Zhuan Zhou
Summary: This study reveals that secretogranin II (SgII), a vesicle matrix protein, plays a role in regulating the size of large dense-core vesicles (LDCVs) through its liquid-liquid phase separation. SgII undergoes pH-dependent polymerization and forms droplets with Ca2+. These droplets recruit lipids similar to LDCV biogenesis. Knockdown of SgII leads to a decrease in neurotransmitter release by affecting LDCV size.
Article
Chemistry, Multidisciplinary
J. Dylan Denison, A. Chathuri De Alwis, Ruby Shah, Gregory S. Mccarty, Leslie A. Sombers
Summary: This study used a planar carbon-fiber microelectrode as a sensor to detect the release dynamics of catecholamine and opioid neuropeptides from chromaffin cells. The data revealed unique kinetics for these two neurochemical classes at the single-vesicle level. The study provides unprecedented insight into key differences between catecholamine and opioid neuropeptide release from isolated chromaffin cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Gregory S. McCarty, Lars E. Dunaway, J. Dylan Denison, Leslie A. Sombers
Summary: This study investigates the reasons why released neurotransmitters can escape detection in amperometric measurements. The results reveal that both diffusional loss and the action of the norepinephrine transporter contribute to the undetected neurotransmitter. This has important implications for understanding the mechanisms of neurotransmitter release.
ANALYTICAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Raed A. Joundi, Amy Y. X. Yu, Eric E. Smith, Charlotte Zerna, Andrew M. Penn, Robert F. Balshaw, Kristine Votova, Maximilian B. Bibok, Melanie Penn, Viera Saly, Janka Hegedus, Shelagh B. Coutts
Summary: The relationship between the duration of transient neurological events and the presence of diffusion-weighted lesions varies depending on symptom type. For focal-only symptoms, there is a continuous increase in the probability of DWI lesion with longer duration, particularly in women. However, for nonfocal or mixed symptoms, the probability of DWI lesion remains constant regardless of duration.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Clinical Neurology
Nicole Schwab, Richard Wennberg, Karl Grenier, Carmela Tartaglia, Charles Tator, Lili-Naz Hazrati
Summary: In this cohort of 35 former collision sports athletes, no significant associations were found between career duration, position played, and role played, and the presence of CTE at autopsy. While limited by the small and nonrepresentative sample studied, these findings suggest that nonsport factors may play a role in determining differing susceptibilities among athletes to CTE.
Article
Environmental Sciences
Sheela Katuwal, Amanda J. Ashworth, Philip R. Owens
Summary: Preferential flow through soil macropores plays a significant role in the rapid transport of contaminants. This study found that soil with a higher fraction of coarse fragments, specifically karst-derived stony soils, drained significantly more water and Br- compared to nonkarst-derived soil. This indicates that soils with a high fraction of coarse fragments are more susceptible to leaching losses.
VADOSE ZONE JOURNAL
(2021)
Article
Endocrinology & Metabolism
Botey Katamu Benjamin, Chunguang Qiu, Zhanying Han, Wenjie Lu, Guoju Sun, Xiaofei Qin, Xule Wang, Xi Wang, Ran Li, Liang Pan
Summary: The study demonstrated that longer diabetes duration is associated with an increased risk of myocardial infarction and repeat coronary revascularization after PCI, but did not significantly affect all-cause mortality.
JOURNAL OF DIABETES RESEARCH
(2021)
Article
Medicine, General & Internal
Scott R. Bauer, Louise C. Walter, Kristine E. Ensrud, Anne M. Suskind, John C. Newman, William A. Ricke, Teresa T. Liu, Kevin T. McVary, Kenneth Covinsky
Summary: This study found that frailty is independently associated with a greater risk of clinical BPH progression and serious adverse events. Older frail men with BPH should be counseled regarding their higher risk of progression despite combination therapy and their likelihood of experiencing serious adverse events regardless of treatment choice.
Article
Infectious Diseases
Rosa Polo, Xabier Garcia-Albeniz, Carolina Teran, Miguel Morales, David Rial-Crestelo, M. Angeles Garcinuno, Miguel Garcia del Toro, Cesar Hita, Juan Luis Gomez-Sirvent, Luis Buzon, Alberto Diaz de Santiago, Jose Perez Arellano, Jesus Sanz, Pablo Bachiller, Elisa Martinez Alfaro, Vicente Diaz-Brito, Mar Masia, Alicia Hernandez-Torres, Jose M. Guerra, Jesus Santos, Piedad Arazo, Leopoldo Munoz, Jose Ramon Arribas, Pablo Martinez de Salazar, Santiago Moreno, Miguel A. Hernan, Julia Del Amo
Summary: This study evaluated the effectiveness of hydroxychloroquine (HCQ) and Tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) as pre-exposure prophylaxis for COVID-19. The results suggest that the use of TDF/FTC and HCQ may have both benefits and risks in preventing COVID-19.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Medicine, Research & Experimental
Veronica Jimenez-Sabado, Sergi Casabella-Ramon, Anna Llach, Ignasi Gich, Sandra Casellas, Francisco Ciruela, S. R. Wayne Chen, Jose M. Guerra, Antonino Ginel, Raul Benitez, Juan Cinca, Carmen Tarifa, Leif Hove-Madsen
Summary: This study aimed to examine the impact of I3-blocker treatment on spontaneous calcium release events or transient inward currents (ITI) in patients with atrial fibrillation (AF). The results showed that I3-blocker treatment did not affect calcium current density, but it reduced ITI frequency and calcium spark frequency, as well as the levels of phosphorylated RyR2 and phospholamban.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Ana Carolina Schwab, Ante Anic, Michal M. Farkowski, Jose Guerra, Konstantinos E. Iliodromitis, Kristine Jubele, Rui Providencia, Julian K. R. Chun, Serge Boveda
Summary: This study conducted a survey to show how clinicians currently approach the knowledge gap in dealing with recurrences after a failed ablation for atrial fibrillation (AF). The majority of respondents perform routine monitoring for AF recurrences after ablation and prefer a long-term monitoring strategy. The combination of absence of symptoms and recorded AF is considered as the definition of success by most respondents.
Article
Physiology
Gerard Amoros-Figueras, Sergi Casabella-Ramon, Georgina Company-Se, Dabit Arzamendi, Esther Jorge, Alvaro Garcia-Osuna, Yolanda Macias, Damian Sanchez-Quintana, Javier Rosell-Ferrer, Jose M. Guerra, Juan Cinca
Summary: A model of atrial infarction was established by selective occlusion of atrial coronary branches. Multifrequency impedance mapping can recognize atrial infarct scarring with lower data variability, which has potential application in the study of atrial arrhythmia mechanisms.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Ana C. Abbott, Isaac E. Garcia, Felipe Villanelo, Carolina Flores-Munoz, Ricardo Ceriani, Jaime Maripillan, Joel Novoa-Molina, Cindel Figueroa-Cares, Tomas Perez-Acle, Juan C. Saez, Helmuth A. Sanchez, Agustin D. Martinez
Summary: It has been demonstrated that a genetic mutation in the gap junction protein Connexin 26 (Cx26) can lead to hyperactivity and damage of hearing cells in the cochlea, resulting in syndromic deafness. The specific mutation Cx26S17F, when co-expressed with wild-type connexins like Cx30, forms hyperactive hemichannels in supporting cells of the organ of Corti. These hyperactive hemichannels are insensitive to blockers and ions, leading to abnormal calcium influx and cellular damage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Lukasz Januszkiewicz, Sergio Barra, Rui Providencia, Julian K. R. Chun, Giulio Conte, Michal M. Farkowski, Jose M. Guerra, Eloi Marijon, Carlo de Asmundis, Serge Boveda
Summary: The aim of this study was to identify potential regional disparities in characteristics of ICD recipients, patient perceptions and perspectives after implantation and level of information provided to patients. The study found that patients in Western Europe reported higher satisfaction after ICD placement, while patients in Central/Eastern Europe felt more optimally informed at the time of device implantation. Therefore, novel strategies are needed to address regional differences in patients' quality of life and provision of information.
Article
Biochemistry & Molecular Biology
Carmen Tarifa, Veronica Jimenez-Sabado, Rafael Franco, Jose Montiel, Jose Guerra, Francisco Ciruela, Leif Hove-Madsen
Summary: Increased activation of adenosine A(2A) receptors (A(2A)Rs) leads to higher incidence of spontaneous calcium release in atrial fibrillation (AF). Adenosine A(3) receptors (A(3)Rs) can counteract excessive A(2A)R activation, but their role in the atrium is not well understood. This study found that A(3)Rs inhibit spontaneous calcium release in human atrial myocytes and can attenuate pathological elevations in calcium release events, suggesting their potential therapeutic importance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Valerie Samouillan, Eduardo Garcia, Aleyda Benitez-Amaro, Maria Teresa La Chica Lhoest, Jany Dandurand, Virginia Actis Dato, Jose Maria Guerra, Joan Carles Escola-Gil, Gustavo Chiabrando, Carlos Enrich, Vicenta Llorente-Cortes
Summary: The accumulation of lipids in cardiomyocytes leads to cardiac dysfunction. Blocking cardiomyocyte cholesteryl ester (CE) loading specifically with antibodies against the P3 sequence of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 antibodies on biophysical alterations was analyzed.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Clinical Neurology
Jorge Arriagada-Diaz, Carolina Flores-Munoz, Barbara Gomez-Soto, Marjorie Labrana-Allende, Michelle Mattar-Araos, Lorena Prado-Vega, Fernando Hinostroza, Ivana Gajardo, Maria Jose Guerra-Fernandez, Jorge A. Bevilacqua, Ana M. Cardenas, Marc Bitoun, Alvaro O. Ardiles, Arlek M. Gonzalez-Jamett
Summary: This study investigates the effect of the dynamin-2 p.R465W mutation on CNS function and suggests that it disrupts synaptic and cognitive function. The mutation leads to changes in neuronal morphology and impaired excitatory synaptic transmission.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
Article
Medicine, General & Internal
Leslie P. Zuniga-Macias, Ricardo E. Ramirez-Orozco, Francisco J. Avelar-Gonzalez, Rosalba Perez-Villalva, Norma A. Bobadilla, Jose M. Arreola-Guerra
Summary: The state of Aguascalientes, Mexico has a high prevalence of chronic kidney disease, particularly persistent albuminuria (pA). This study aimed to identify potential biomarkers of early renal injury in pA patients. The results showed that SerpinA3 was significantly associated with pA and could be a biomarker for early kidney injury.
REVISTA DE INVESTIGACION CLINICA-CLINICAL AND TRANSLATIONAL INVESTIGATION
(2023)
Review
Biochemistry & Molecular Biology
Claudia Garcia-Rodriguez, Paula Mujica, Javiera Illanes-Gonzalez, Araceli Lopez, Camilo Vargas, Juan C. Saez, Arlek Gonzalez-Jamett, Alvaro O. Ardiles
Summary: Probenecid, an old uricosuric agent, has been used to treat gout and reduce renal excretion of antibiotics. Recent studies have shown that Probenecid has the ability to interact with membrane proteins, indicating potential therapeutic applications in medicine. It has been found to have neuroprotective, antiepileptic, and anti-inflammatory properties, making it useful in the treatment of neurological and neurodegenerative diseases. Despite its declining clinical use, Probenecid shows promise in preclinical research as a strategy to enhance drug bioavailability in the central nervous system.
Article
Medicine, General & Internal
Carolina C. Barajas-Garcia, Alma L. Guerrero-Barrera, Jose M. Arreola-Guerra, Francisco J. Avelar-Gonzalez, Flor Y. Ramirez-Castillo
Summary: We present two cases of postrenal transplant patients with large biofilm structures covering the double-J stents, without urinary tract infections. The biofilms consisted of cocci integrated in a net structure and overlapping bacilli cells. This is the first time high-quality images of noncrystalline biofilms in renal transplant recipients with long-term stenting have been reported.
JOURNAL OF MEDICAL CASE REPORTS
(2023)
Article
Fisheries
M. Castilla-Gavilan, J. M. Guerra-Garcia, J. M. Moreno-Oliva, I. Hachero-Cruzado
Summary: Marine amphipods, specifically Gammarus insensibilis, have shown potential as a live feed resource for Integrated Multi-Trophic Aquaculture. This study examined the waste removal capabilities of G. insensibilis at different densities and fed on dry or wet detritus. The species exhibited high survival rates and showed the highest tolerance for nitrate, followed by nitrite and ammonium. Low-density individuals were found to remove significantly higher amounts of detritus, regardless of its dry or wet state. Wet detritus is recommended for better handling and scaled-up trials in aquaculture systems.
Article
Cardiac & Cardiovascular Systems
Gerard Amoros-Figueras, Sergi Casabella-Ramon, Zoraida Moreno-Weidmann, Antoni Ivorra, Jose M. Guerra, Tomas Garcia-Sanchez
Summary: This study characterizes the dynamics of electrograms after pulsed field ablation (PFA) in areas of irreversible electroporation (IRE) and reversible electroporation. The results show that electrograms can differentiate between reversible electroporation and IRE areas during the first 30 minutes post ablation, and provide valuable information for immediate lesion assessment after PFA.
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2023)
Article
Clinical Neurology
Jorge Arriagada-Diaz, Carolina Flores-Munoz, Barbara Gomez-Soto, Marjorie Labrana-Allende, Michelle Mattar-Araos, Lorena Prado-Vega, Fernando Hinostroza, Ivana Gajardo, Maria Jose Guerra-Fernandez, Jorge A. Bevilacqua, Ana M. Cardenas, Marc Bitoun, Alvaro O. Ardiles, Arlek M. Gonzalez-Jamett
Summary: This study investigated the effect of a mutation in the dynamin-2 gene on central nervous system function. The mutation was found to reduce dendritic arborisation and spine density, as well as impair excitatory synaptic transmission and recognition memory in a mouse model. These findings highlight the role of dynamin-2 in regulating neuronal morphology and synaptic transmission in the hippocampus.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
