期刊
JOURNAL OF NEUROSCIENCE
卷 29, 期 31, 页码 9668-9682出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0362-09.2009
关键词
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资金
- Canadian Institutes of Health Research
- Tier 1 Canada Research Chair in Biotechnology and Genomics-Neurobiology
- Centre National pour la Recherche Scientifique
- L'Ecole Normale Superieure
- L'Agence Nationale pour La Recherche and Human Frontier Science Program
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [17023021, 17100004]
- Natural Sciences and Engineering Research Council of Canada
- Michael Smith Foundation for Health Research
- Grants-in-Aid for Scientific Research [17100004, 17023021] Funding Source: KAKEN
T-type voltage-gated calcium channels are expressed in the dendrites of many neurons, although their functional interactions with postsynaptic receptors and contributions to synaptic signaling are not well understood. We combine electrophysiological and ultrafast two-photon calcium imaging to demonstrate that mGluR1 activation potentiates cerebellar Purkinje cell Ca(v)3.1 T-type currents via a G-protein-and tyrosine-phosphatase-dependent pathway. Immunohistochemical and electron microscopic investigations on wild-type and Ca(v)3.1 gene knock-out animals show that Ca(v)3.1 T-type channels are preferentially expressed in Purkinje cell dendritic spines and colocalize with mGluR1s. We further demonstrate that parallel fiber stimulation induces fast subthreshold calcium signaling in dendritic spines and that the synaptic Ca(v)3.1-mediated calcium transients are potentiated by mGluR1 selectively during bursts of excitatory parallel fiber inputs. Our data identify a new fast calcium signaling pathway in Purkinje cell dendritic spines triggered by short burst of parallel fiber inputs and mediated by T-type calcium channels and mGluR1s.
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