期刊
JOURNAL OF NEUROSCIENCE
卷 28, 期 48, 页码 12798-12807出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4101-08.2008
关键词
tau phosphorylation; microtubules; Alzheimer's disease; anesthesia; hypothermia; tubulin
资金
- National Institute of Neurological Disorders and Stroke [NS048447]
- National Institute of Aging [AG017216]
In Alzheimer's disease, tau is hyperphosphorylated, which is thought to detach it from microtubules (MTs), induce MT destabilization, and promote aggregation. Using a previously described in vivo model, we investigated whether hyperphosphorylation impacts tau function in wild-type and transgenic mice. We found that after anesthesia-induced hypothermia, MT-free tau was hyperphosphorylated, which impaired its ability to bind MTs and promote MT assembly. MT-bound tau was more resistant to hyperphosphorylation compared with free tau and tau did not dissociate from MTs in wild-type mice. However, 3-repeat tau detached from MT in the transgenic mice. Surprisingly, dissociation of tau from MTs did not lead to overt depolymerization of tubulin, and there was no collapse, or disturbance of axonal MT networks. These results indicate that, in vivo, a subpopulation of tau bound to MTs does not easily dissociate under conditions that extensively phosphorylate tau. Tau remaining on the MTs under these conditions is sufficient to maintain MT network integrity.
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