Article
Neurosciences
Sung Jin Park, Kirk D. Haan, Yoshikazu Nakamura, Kiyoko Fukami, Thomas E. Fisher
Summary: The study revealed that the PLC delta 1 isoform plays a crucial role in the activation of Delta N-TRPV1 channels and osmosensory transduction in MNCs, with its absence leading to impaired MNC function. This research advances our understanding of the molecular mechanisms of osmoregulation in mammals.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Hao-Hua Wei, Xiang-Shan Yuan, Ze-Ka Chen, Pei-Pei Chen, Zhe Xiang, Wei-Min Qu, Rui-Xi Li, Guo-Min Zhou, Zhi-Li Huang
Summary: The AVP neurons in SON and PVN receive similar input patterns from multiple brain regions, with PVNAVP neurons having broader and denser inputs compared to SONAVP neurons. Additionally, differentiated input sources were found, with SONAVP neurons receiving more projections from thermoregulatory nuclei and PVNAVP neurons receiving more afferent inputs from circadian rhythm-regulating nuclei. The study provides insights into the distinct afferent framework of AVP neurons in SON and PVN, laying the foundation for further research on their diverse roles.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Neurosciences
Mehwish Abbasi, Michael R. Perkinson, Alexander J. Seymour, Richard Piet, Rebecca E. Campbell, Karl J. Iremonger, Colin H. Brown
Summary: The research found that kisspeptin can directly activate oxytocin neurons during late pregnancy by increasing post-spike excitability. This study is important for understanding the mechanism of kisspeptin's role in pregnancy.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Neurosciences
Yun-Hao Jiang, Tong Li, Yang Liu, Xiaoyu Liu, Shuwei Jia, Chunmei Hou, Guichuan Chen, Hongyang Wang, Shuo Ling, Qiang Gao, Xiao-Ran Wang, Yu-Feng Wang
Summary: Astrocytic morphological plasticity and modulation of adjacent neuronal activity depend on astrocytic volume regulation, which is controlled by glial fibrillary acidic protein (GFAP), aquaporin 4 (AQP4), and potassium channels including inwardly rectifying K+ channel 4.1 (Kir4.1). This study reveals the associations between Kir4.1 and other molecules in astrocytic volume regulation and their impact on neuronal activity. The results suggest that hypotonic challenge (HOC) leads to astrocytic retraction and decreased volume, accompanied by increased Kir4.1 activity. Kir4.1 involvement in HOC-induced astrocytic retraction depends on AQP4 activity and GFAP plasticity, which ultimately affects the excitability of vasopressin (VP) neurons.
Article
Biology
Panjiao Lin, Benjamin T. Gillard, Audrys G. Pauza, Fernando A. Iraizoz, Mahmoud A. Ali, Andre S. Mecawi, Fatma Z. Djazouli Alim, Elena Romanova, Pamela A. Burger, Michael P. Greenwood, Abdu Adem, David Murphy
Summary: This study focuses on the mechanisms of adaptive response to osmotic stress in the camel supraoptic nucleus, aiming to reveal the central control of water homeostasis in camels. By building three-dimensional models and comparing transcriptomic data, we identified unique adaptive genes in camels and common elements in the water deprivation transcriptomic response network.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
David Levi, Joshua C. Wyrosdic, Amirah-Iman Hicks, Mary Ann Andrade, Glenn M. Toney, Masha Prager-Khoutorsky, Charles W. Bourque
Summary: High dietary salt increases arterial pressure partly through activating MNCVP cells that secrete the antidiuretic and vasoconstrictor hormone vasopressin. In two rat models of salt-dependent hypertension, the intrinsic and synaptic excitation of MNCVP caused by hypertonicity was differentially potentiated. Dietary salt increases the excitability of MNCVP through effects on the cell-autonomous and synaptic osmoresponsiveness of MNCVP.
Article
Multidisciplinary Sciences
Matthew K. Kirchner, Ferdinand Althammer, Kevin J. Donaldson, Daniel N. Cox, Javier E. Stern
Summary: This study investigated the trafficking dynamics of large dense core vesicles (LDCVs) in vasopressin neurons using two-photon imaging. The results revealed differential regulation of axonal and dendritic LDCV trafficking and suggested that changes in trafficking dynamics constitute a novel mechanism for peptidergic neurons to efficiently adapt to increased hormonal demand.
Article
Cell Biology
Theresa A. Chmiel, Margaret L. Gardel
Summary: Epithelial cell volume regulation is crucial for tissue stability and dynamics. While individual mammalian cells use NKCC channels to maintain volume homeostasis under hyperosmotic stress, mature epithelial tissue behaves differently, with cell volume decreasing when subjected to hyperosmotic stress. The tight junction protein ZO-1 and Rho-associated kinase ROCK are essential for osmotic regulation of cell volume in mature epithelium.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Endocrinology & Metabolism
V. Trujillo, F. R. Mir, M. M. Su, L. Vivas
Summary: The study shows that early maternal separation has long-term effects on basal and hyperosmolarity-induced Avp hypothalamic expression, as well as on the intrinsic excitability of magnocellular supraoptic neurons.
PSYCHONEUROENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Jessica R. Sudbury, Cristian Zaelzer, Eric Trudel, Anna Bumagin, Charles W. Bourque
Summary: Increases in core body temperature lead to the secretion of antidiuretic hormones to promote water absorption and reduce water loss. Our study found that the OVLT acts as an important thermosensory nucleus and plays a crucial role in activating MNCs during physiological heating.
JOURNAL OF NEUROENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bibiana Torok, Peter Csikota, Anna Fodor, Diana Balazsfi, Szilamer Ferenczi, Kornel Demeter, Zsuzsanna E. Toth, Katalin Konczol, Judith Camats Perna, Imre Farkas, Krisztina J. Kovacs, Jozsef Haller, Mario Engelmann, Dora Zelena
Summary: The study demonstrates that rescuing magnocellular AVP synthesis with AVP-AAV treatment affects endocrine stress response and social behavior, potentially suggesting a key role of AVP in regulating the stress axis and modulating social behavior, particularly in response to stressors and social interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Michael R. Perkinson, Rachael A. Augustine, Gregory T. Bouwer, Emily F. Brown, Isaiah Cheong, Alexander J. Seymour, Martin Fronius, Colin H. Brown
Summary: Oxytocin and vasopressin secretion are crucial for pregnancy and lactation, with oxytocin promoting uterus contractions and milk ejection, while vasopressin helps retain water for the demands of pregnancy and lactation. The excitability of oxytocin neurons is increased in late pregnancy and lactation, leading to higher frequency firing, while the activity of vasopressin neurons remains consistent despite lower osmolality. Delta N-TRPV1 channels may play a role in maintaining vasopressin neuron activity for water retention during pregnancy and lactation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Daan Vorselen, Sarah R. Barger, Yifan Wang, Wei Cai, Julie A. Theriot, Nils C. Gauthier, Mira Krendel
Summary: The study reveals that during macrophage phagocytosis of antibody-opsonized targets, there is a prominent constriction driven by Arp2/3-mediated assembly of discrete actin protrusions. Contractile myosin-II activity contributes to late-stage phagocytic force generation and target ingestion. Observations of partial target eating attempts and sudden target release suggest that constriction may play a critical role in resolving complex in vivo target encounters.
Article
Neurosciences
Akie Toya, Masahide Fukada, Eiko Aoki, Tohru Matsuki, Masashi Ueda, Shima Eda, Yoshio Hashizume, Akio Iio, Shigeo Masaki, Atsuo Nakayama
Summary: NLGN4X has been identified as the causative gene for familial nonsyndromic autism. Its function is not fully understood due to the lack of a similar gene in rodents. Recent studies have shown that NLGN4 is abundantly expressed in the cerebral cortex, mainly at excitatory synapses. However, the detailed histological distribution of NLGN4, which may be important for its relationship with autistic phenotypes, has not been clarified.
Article
Chemistry, Multidisciplinary
Martin R. Gill, Paul J. Jarman, Vanessa Hearnden, Simon D. Fairbanks, Marcella Bassetto, John Palmer, Kathryn R. Ayscough, Jim A. Thomas, Carl Smythe
Summary: RuRuPhen blocks the transformation of G-actin monomers to F-actin filaments and disrupts the organization of actin stress fibers in cells. It interferes with actomyosin contractility, cell motility, and late-stage cytokinesis. RuRuPhen also interferes with the recruitment of endosomal sorting complexes required for transport (ESCRT) complex, leading to cytokinetic abscission failure.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Physiology
Masha Prager-Khoutorsky, Charles W. Bourque
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2015)
Article
Endocrinology & Metabolism
M. Prager-Khoutorsky, C. W. Bourque
JOURNAL OF NEUROENDOCRINOLOGY
(2015)
Article
Cell Biology
Tina Djogo, Sarah C. Robins, Sarah Schneider, Darya Kryzskaya, Xiaohong Liu, Andrew Mingay, Colleen J. Gillon, Joo Hyun Kim, Kai-Florian Storch, Ulrich Boehm, Charles W. Bourque, Thomas Stroh, Leda Dimou, Maia V. Kokoeva
Article
Endocrinology & Metabolism
K. Y. Choe, M. Prager-Khoutorsky, W. T. Farmer, K. K. Murai, C. W. Bourque
JOURNAL OF NEUROENDOCRINOLOGY
(2016)
Article
Multidisciplinary Sciences
C. Gizowski, C. Zaelzer, C. W. Bourque
Article
Multidisciplinary Sciences
Arkady Khoutorsky, Robert E. Sorge, Masha Prager-Khoutorsky, Sophie Anne Pawlowski, Geraldine Longo, Seyed Mehdi Jafarnejad, Soroush Tahmasebi, Loren J. Martin, Mark H. Pitcher, Christos G. Gkogkas, Reza Sharif-Naeini, Alfredo Ribeiro-da-Silva, Charles W. Bourque, Fernando Cervero, Jeffrey S. Mogil, Nahum Sonenberg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
W. Todd Farmer, Therese Abrahamsson, Sabrina Chierzi, Christopher Lui, Cristian Zaelzer, Emma V. Jones, Blandine Ponroy Bally, Gary G. Chen, Jean-Francois Theroux, Jimmy Peng, Charles W. Bourque, Frederic Charron, Carl Ernst, P. Jesper Sjoestroem, Keith K. Murai
Review
Urology & Nephrology
Claire Gizowski, Charles W. Bourque
NATURE REVIEWS NEPHROLOGY
(2018)
Editorial Material
Multidisciplinary Sciences
Claire Gizowski, Charles W. Bourque
Review
Endocrinology & Metabolism
C. Gizowski, C. Zaelzer, C. W. Bourque
JOURNAL OF NEUROENDOCRINOLOGY
(2018)
Article
Neurosciences
Cristian Zaelzer, Claire Gizowski, Christopher K. Salmon, Keith K. Murai, Charles W. Bourque
JOURNAL OF NEUROPHYSIOLOGY
(2018)
Article
Cell Biology
Sorana Ciura, Masha Prager-Khoutorsky, Zahra S. Thirouin, Joshua C. Wyrosdic, James E. Olson, Wolfgang Liedtke, Charles W. Bourque
Review
Physiology
Claire Gizowski, Charles W. Bourque
CURRENT OPINION IN PHYSIOLOGY
(2018)
Article
Endocrinology & Metabolism
Claire Gizowski, Eric Trudel, Charles W. Bourque
BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM
(2017)
Review
Peripheral Vascular Disease
Masha Prager-Khoutorsky, Katrina Y. Choe, David I. Levi, Charles W. Bourque
CURRENT HYPERTENSION REPORTS
(2017)