Article
Ophthalmology
Jianguo Fan, Dinusha Rajapakse, Katherine Peterson, Joshua Lerner, Shabnam Parsa, Arjun Ponduri, Vatsala Sagar, Todd Duncan, Lijin Dong, Graeme Wistow
Summary: Vision requires the transport and recycling of the pigment 11-cis retinaldehyde between the retinal pigment epithelium and photoreceptors. Retbindin, a photoreceptor-specific protein, plays an important role in mediating light damage and regeneration of retinal function. Rtbdn-KO mice lacking retbindin show delayed regeneration of retinal function and resistance to light-induced photoreceptor death, suggesting a crucial role of retbindin in the mammalian visual cycle.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Editorial Material
Ophthalmology
Siddharth Patel, Devesh Kumawat, Shrinkhal, Nisheeta Patnaik, Arnab Garai, Ramanuj Samanta, Anupam Singh
Summary: This case reports a rare case of bilateral extensive serous pigment epithelium detachments (PEDs) with blister-like elevations. The patient presented with sudden onset diminution of vision in the left eye. After treatment, the patient's visual acuity improved.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daniela F. Santos, Mariana Pais, Claudia N. Santos, Gabriela A. Silva
Summary: Studies have shown that the pyrogallol-O-sulfate metabolite can reduce the expression of pro-angiogenic VEGF in diabetic retinopathy, serving as a potential preventive approach against disease progression. Additionally, it also decreases the expression of inflammatory marker Iba1, highlighting its dual role in targeting molecules involved in both inflammation and neovascularization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Anatomy & Morphology
Debora Napoli, Enrica Strettoi
Summary: Retinal pigment epithelium (RPE) is a specialized cell layer dedicated to supporting and protecting the retina. In retinitis pigmentosa (RP), degeneration of the photoreceptors can lead to abnormal organization and compromised barrier function of RPE cells. The biological mechanisms behind these changes are largely unknown. A study using a mouse model of RP suggests that inflammatory responses may be involved in remodeling of RPE, and further investigation is needed.
JOURNAL OF ANATOMY
(2023)
Article
Biotechnology & Applied Microbiology
Huili Li, Ming Zhang, Dahong Wang, Guojun Dong, Zhiwei Chen, Suilin Li, Xiaohong Sun, Min Zeng, Haiyang Liao, Huifang Chen, Shengyan Xiao, Xiaodan Li
Summary: The clinical and animal experiments indicate that low-intensity blue light can cause structural and functional damage to the retina, warranting attention to chronic damage caused by low-intensity light.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Kai-Chun Cheng, Yun-Tzu Hsu, Wangta Liu, Huey-Lan Huang, Liang-Yu Chen, Chen-Xi He, Shwu-Jiuan Sheu, Kuo-Jen Chen, Po-Yen Lee, Yi-Hsiung Lin, Chien-Chih Chiu
Summary: The study revealed that blue light exposure leads to decreased cell proliferation, increased apoptosis, oxidative stress, DNA damage activation, and autophagy in age-related macular degeneration. Zebrafish larvae model demonstrated the unfavorable impact of blue light on retinal tissue development. RNA-Seq results confirmed that blue light induced cell death and participated in tissue growth inhibition and maturation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Qin Ke, Lili Gong, Xingfei Zhu, Ruili Qi, Ming Zou, Baoxin Chen, Wei Liu, Shan Huang, Yizhi Liu, David Wan-Cheng Li
Summary: This study reveals an association between multinucleated RPE cells and nocturnal vision, and finds that these cells exhibit increased ROS production and DNA damage after X-ray irradiation. Furthermore, the study shows that changes in p53 dosage affect the DNA damage response, with mononucleated RPE cells being more sensitive to these changes.
Article
Biochemistry & Molecular Biology
Debora Napoli, Martina Biagioni, Federico Billeri, Beatrice Di Marco, Noemi Orsini, Elena Novelli, Enrica Strettoi
Summary: The study reveals structural abnormalities in the RPE of RP patients, leading to dysfunction of the inner barrier. Findings also suggest a correlation between the extent of photoreceptor loss and interruption zones of ZO-1. Treatment with dexamethasone in RP mice not only rescues cones but also restores RPE structure, highlighting a potential feedback loop for simultaneous protection of cones and the RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Liling Ou, Xiujuan Lv, Zixia Wu, Weibo Xia, Yida Huang, Luya Chen, Wenjie Sun, Yao Qi, Mei Yang, Lei Qi
Summary: The study investigated the genotoxicity of graphene oxide (GO) and reduced graphene oxide (RGO) towards human retinal pigment epithelium (RPE) cells, showing that both materials induced ROS-dependent DNA damage. The results also suggested that reducing the saturated C-O bonds in GO enhanced DNA damage, indicating the potential of reducing the toxicity of graphene-based materials through chemical modification.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Dai Le, Soyeon Lim, Kwang Wook Min, Joon Woo Park, Youjoung Kim, Taejeong Ha, Kyeong Hwan Moon, Kay-Uwe Wagner, Jin Woo Kim
Summary: The distribution of membrane proteins in RPE varies during different developmental stages, indicating developmental regulation of protein trafficking. Deletion of Tsg101 disrupts RPE polarity, leading to irregular aggregates and non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling, highlighting the importance of ESCRT-mediated protein trafficking for RPE cell polarity development and maintenance.
MOLECULES AND CELLS
(2021)
Article
Geriatrics & Gerontology
Nan Su, Uwe Hansen, Tanja Plagemann, Karin Gaeher, M. Dominik Leclaire, Jeannette Konig, Annika Hoehn, Tilman Grune, Constantin E. Uhlig, Nicole Eter, Peter Heiduschka
Summary: LF injection into the subretinal space of mice leads to activation and migration of microglial cells, increased expression of inflammatory cytokines, and gradual degradation of RPE cells. These features are consistent with those found in an aging retina, suggesting that LF injection could serve as a model for intermediate and late AMD.
Article
Multidisciplinary Sciences
Jacob S. Heng, Amir Rattner, Genevieve L. Stein-O'Brien, Briana L. Winer, Bryan W. Jones, Hilary J. Vernon, Loyal A. Goff, Jeremy Nathans
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biology
Chris Cho, Yanshu Wang, Philip M. Smallwood, John Williams, Jeremy Nathans
Article
Biology
Yanshu Wang, Mark F. Sabbagh, Xiaowu Gu, Amir Rattner, John Williams, Jeremy Nathans
Article
Multidisciplinary Sciences
Xi Peng, John Williams, Philip M. Smallwood, Jeremy Nathans
Article
Medicine, Research & Experimental
Amir Rattner, John Williams, Jeremy Nathans
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Biochemistry & Molecular Biology
Yanshu Wang, Philip M. Smallwood, John Williams, Jeremy Nathans
HUMAN MOLECULAR GENETICS
(2020)
Article
Multidisciplinary Sciences
Jacob S. Heng, Sean F. Hackett, Genevieve L. Stein-O'Brien, Briana L. Winer, John Williams, Loyal A. Goff, Jeremy Nathans
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biology
Mark F. Sabbagh, Jeremy Nathans
Article
Multidisciplinary Sciences
Tao-Hsin Chang, Fu-Lien Hsieh, Philip M. Smallwood, Sandra B. Gabelli, Jeremy Nathans
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biology
Amir Rattner, Chantelle E. Terrillion, Claudia Jou, Tina Kleven, Shun Felix Hu, John Williams, Zhipeng Hou, Manisha Aggarwal, Susumu Mori, Gloria Shin, Loyal A. Goff, Menno P. Witter, Mikhail Pletnikov, Andre A. Fenton, Jeremy Nathans
Article
Biology
Jie Wang, Amir Rattner, Jeremy Nathans
Summary: This study provides foundational data on mouse iris cell types through single nucleus RNA sequencing, identifying new cell types and revealing transcriptome differences in different states. It could serve as a reference point for further investigations on iris development, disease, and pharmacology.
Review
Neurosciences
Amir Rattner, Yanshu Wang, Jeremy Nathans
Summary: This review discusses the mechanisms by which signals from neurons and glia control the vascular development in the central nervous system (CNS). The vascular endothelial growth factor (VEGF) and beta-catenin (canonical Wnt) signaling are highlighted as the two best-understood systems that regulate CNS vascular development. The review emphasizes foundational discoveries, interactions between different signaling systems, the integration of genetic and cell biological studies, advances that are of clinical relevance, and provides questions for future research.
ANNUAL REVIEW OF NEUROSCIENCE
(2022)
Article
Biology
Jie Wang, Amir Rattner, Jeremy Nathans
Summary: Bacterial meningitis, particularly in infants and the elderly, can lead to serious illness and death. This study investigates the response of different types of meningeal cells to early postnatal E. coli infection in mice. The findings show that the transcriptomes of major meningeal cell types, including endothelial cells, macrophages, and fibroblasts, undergo distinct changes upon infection. Additionally, the study reveals alterations in the distribution of certain proteins and reduced blood-brain barrier integrity in leptomeningeal capillaries. The response to infection is largely driven by TLR4 signaling, and the EC response to E. coli infection is independent of the interaction between macrophages and ECs.
Article
Multidisciplinary Sciences
Amir Rattner, Jacob S. Heng, Briana L. Winer, Loyal A. Goff, Jeremy Nathans
Summary: This study analyzed the cell types and gene expression patterns in the lacrimal gland of mice. It identified a new cell type, Car6+ cells, and found that acinar cells express multiple tear fluid components with sex differences in expression. The study also revealed heterogeneity in gene expression within cell types and identified interferon responses and immune cell infiltrates in Sjogren's syndrome models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
