期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 106, 期 6, 页码 3056-3066出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00544.2011
关键词
transient receptor potential melastatin 8; phosphatidylinositol 4,5-bisphosphate; menthol; rat
资金
- National Institute of Dental and Craniofacial Research [DE-018661]
Sarria I, Ling J, Zhu MX, Gu JG. TRPM8 acute desensitization is mediated by calmodulin and requires PIP 2 : distinction from tachyphylaxis. J Neurophysiol 106: 3056-3066, 2011. First published September 7, 2011; doi: 10.1152/jn.00544.2011.-The cold-sensing channel transient receptor potential melastatin 8 (TRPM8) features Ca2+-dependent downregulation, a cellular process underlying somatosensory accommodation in cold environments. The Ca2+-dependent functional downregulation of TRPM8 is manifested with two distinctive phases, acute desensitization and tachyphylaxis. Here we show in rat dorsal root ganglion neurons that TRPM8 acute desensitization critically depends on phosphatidylinositol 4,5-bisphosphate (PIP2) availability rather than PIP2 hydrolysis and is triggered by calmodulin activation. Tachyphylaxis, on the other hand, is mediated by phospholipase hydrolysis of PIP2 and protein kinase C/phosphatase 1,2A. We further demonstrate that PIP2 switches TRPM8 channel gating to a high-open probability state with short closed times. Ca2+-calmodulin reverses the effect of PIP2, switching channel gating to a low-open probability state with long closed times. Thus, through gating modulation, Ca2+-calmodulin provides a mechanism to rapidly regulate TRPM8 functions in the somatosensory system.
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