期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 105, 期 5, 页码 2260-2274出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00516.2010
关键词
supersensitivity; medium spiny neurons; D(3)nf; 6-hydroxydopamine; Parkinson's disease
资金
- Programa de Investigacion Multidisciplinaria de Proyectos Universitarios de Liderazgo y Superacion Academica (IMPULSA) [03]
- Direccion General de Asuntos del Personal Academico (DGAPA) [IN-205610, IN-206010]
- The Miguel Aleman A. C. Foundation
- Mexico-Germany Agreement Consejo Nacional de Ciencia y Tecnologia-Deutsche Forschungsgemeinschaft (CONACyT-DFG) [I0110/193/10 FON.INST.-29-10, 98004]
- CONACYT (Mexico)
- Fundacion Alberto y Dolores Andrade
Prieto GA, Perez-Burgos A, Palomero-Rivero M, Galarraga E, Drucker-Colin R, Bargas J. Upregulation of D-2-class signaling in dopamine-denervated striatum is in part mediated by D-3 receptors acting on Ca(V)2.1 channels via PIP2 depletion. J Neurophysiol 105: 2260-2274, 2011. First published March 9, 2011; doi:10.1152/jn.00516.2010.-The loss of dopaminergic neurons in the substantia nigra compacta followed by striatal dopamine depletion is a hallmark of Parkinson's disease. After dopamine depletion, dopaminergic D-2 receptor (D2R)-class supersensitivity develops in striatal neurons. The supersensitivity results in an enhanced modulation of Ca2+ currents by D2R-class receptors. However, the relative contribution of D2R, D3R, and D4R types to the supersensitivity, as well as the mechanisms involved, have not been elucidated. In this study, whole cell voltage-clamp recordings were performed to study Ca2+ current modulation in acutely dissociated striatal neurons obtained from rodents with unilateral 6-hydroxydopamine lesions in the substantia nigra compacta. Selective antagonists for D2R, D3R, and D4R types were used to identify whether the modulation by one of these receptors experiences a selective change after dopaminergic denervation. It was found that D3R-mediated modulation was particularly enhanced. Increased modulation targeted Ca(V)2.1 (P/Q) Ca2+ channels via the depletion of phosphatidylinositol 4,5-bisphosphate, an intracellular signaling cascade hard to detect in control neurons and hypothesized as being amplified by dopamine depletion. An imbalance in the striatal expression of D3R and its splice variant, D(3)nf, accompanied enhanced D3R activity. Because Ca(V)2.1 Ca2+ channels mediate synaptic GABA release from the terminals of striatal neurons, reinforcement of their inhibition by D3R may explain in part the profound decrease in synaptic strength in the connections among striatal projection neurons observed in the dopamine-depleted striatum.
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