Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Review
Spectroscopy
Wendy H. Mueller, Alexandre Verdin, Edwin De Pauw, Cedric Malherbe, Gauthier Eppe
Summary: Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a technique that uses nanostructured substrates to promote the desorption/ionization of analytes, allowing for both untargeted and targeted analysis. This review focuses on SALDI-MS imaging (SALDI-MSI), discussing the nomenclature, fundamental mechanisms, analytical strategies, and applications in various fields. SALDI-MSI has strengths and limitations, but shows promising prospects for future development.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Gabriel R. Martins, Felipe L. Brum, Davi Marconi Miranda Carvalho da Silva, Livia C. Barbosa, Ronaldo Mohana-Borges, Ayla Sant'Ana da Silva
Summary: Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a valuable technique for identifying compounds and understanding their physiological information in plant tissues. Researchers have developed a sample preparation protocol to obtain thin sections of hard seeds, enabling molecular mapping by MALDI-IMS. This method has the potential to be a valuable tool for studying the morphology, anatomy, and physiology of botanical materials.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Dong Chen, Mingyi Du, Yudi Huang, Yizhu Xu, Yingying Chen, Lianlian Ma, Qingrong Xie, Xinhai Zhu, Zilong Chen, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: Mass spectrometry imaging (MSI) enables the characterization of small-molecule metabolites in biological tissues. However, existing techniques suffer from background interference and inhomogeneous matrix deposition. This study presents a new composite substrate that overcomes these limitations and demonstrates superior performance for the detection and imaging of small biomolecules.
Article
Chemistry, Physical
Dong Chen, Mingyi Du, Yudi Huang, Yizhu Xu, Yingying Chen, Lianlian Ma, Qingrong Xie, Xinhai Zhu, Zilong Chen, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: Mass spectrometry imaging (MSI) enables the characterization of small-molecule metabolites within biological tissues. However, current matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has limitations. Surface-assisted LDI-MS (SALDI-MS) using the AuNP-hPDA-TDNT substrate shows superior performance for the detection and imaging of small biomolecules. The substrate has reduced background interference and allows visualization of various metabolites and lipids.
Article
Chemistry, Analytical
Ge Ma, Xiaoyong Zhao, Mengzhe Guo, Yaqin Liu, Keqiang Shi, Cheng Guo, Yuanjiang Pan
Summary: In this study, a matrix glycosylation strategy was proposed to improve the sensitivity and signal reproducibility of MALDI MS analysis. The glycosylated matrices, especially 6-GAQ, exhibited remarkably-improved performance in detecting metabolites and imaging small molecules in complex samples. The glycosylation strategy showed great potential in the field of mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Chi-Sheng Chen, Wen-Chi Chen, Sarah Y. Chang
Summary: A new mode of electromembrane extraction (EME) has been developed for detection via matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS). The method successfully extracted and analyzed posaconazole, and was applied to human serum samples.
Article
Nanoscience & Nanotechnology
Mingyi Du, Dong Chen, Yingying Chen, Yudi Huang, Lianlian Ma, Qingrong Xie, Yizhu Xu, Xinhai Zhu, Zilong Chen, Zhibin Yin, Hanhong Xu, Xinzhou Wu
Summary: In this study, SiO2@Au nanoshells were optimized for high-sensitivity LDI-MS analysis and a wide range of MSI applications, demonstrating superior performance in the detection and imaging of various small molecular metabolites. The method showed significant reduction in matrix interference, increased analyte coverage, lower detection limits, and good repeatability.
ACS APPLIED NANO MATERIALS
(2022)
Article
Neurosciences
Srinivas Koutarapu, Junyue Ge, Durga Jha, Kaj Blennow, Henrik Zetterberg, Tammaryn Lashley, Wojciech Michno, Jorg Hanrieder
Summary: Alzheimer's disease is the most common neurodegenerative disease, and its underlying pathogenic mechanisms are not fully understood. The study focuses on the main pathological hallmarks of the disease, particularly beta-amyloid plaque pathology. By using advanced imaging techniques, the research identified distinct A beta signatures and key A beta species associated with neuritic dystrophy.
BRAIN CONNECTIVITY
(2023)
Article
Clinical Neurology
Charlotte Johansson, Steinunn Thordardottir, Jose Laffita-Mesa, Elena Rodriguez-Vieitez, Henrik Zetterberg, Kaj Blennow, Caroline Graff
Summary: The study describes plasma biomarkers in a Swedish cohort of patients with monogenic Alzheimer's disease. Plasma GFAP increases before P-tau181 and NfL, indicating that it reflects early Alzheimer's disease pathology. Plasma biomarkers may be non-invasive tools to detect Alzheimer's disease-related abnormalities. However, further validation is needed.
Article
Medicine, General & Internal
Enrico Ripamonti, Arvid Eden, Staffan Nilsson, Anders Sonnerborg, Henrik Zetterberg, Magnus Gisslen
Summary: This retrospective follow-up study investigates the longitudinal change of plasma neurofilament light (NfL) levels after antiretroviral therapy (ART) initiation in people living with HIV. The results show that plasma NfL levels are higher at baseline and decline faster during the follow-up in patients with CD4(+) count 100 cells/μl or higher. There is no significant correlation between baseline HIV RNA levels, treatment regimen, and sex with plasma NfL levels.
JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Microbiology
Laura P. Kincer, Sarah Beth Joseph, Maria M. Gilleece, Blake M. Hauser, Sabrina Sizemore, Shuntai Zhou, Clara Di Germanio, Henrik Zetterberg, Dietmar Fuchs, Steven G. Deeks, Serena Spudich, Magnus Gisslen, Richard W. Price, Ronald Swanstrom
Summary: HIV-1 can persist in a latent reservoir in individuals receiving antiretroviral therapy, and rebound virus can be detected in the cerebrospinal fluid during treatment interruption. In this study, we found that high viral loads and clonally amplified viral lineages in the cerebrospinal fluid were correlated with the influx of white blood cells. Additionally, we did not observe rebound macrophage-tropic virus in the cerebrospinal fluid, indicating that the CNS may not be a source of this virus. We propose a model in which R5 T cell-tropic virus is released from infected T cells in the CNS during treatment interruption.
NATURE MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Junyue Ge, Srinivas Koutarapu, Durga Jha, Maciej Dulewicz, Henrik Zetterberg, Kaj Blennow, Joerg Hanrieder
Summary: Beta-amyloid plaque pathology is a prominent feature of Alzheimer's disease, but the mechanisms linking it to the disease are not fully understood. A novel imaging approach was used to investigate the correlation between lipids and amyloid plaques in mouse models of AD. The results identified characteristic patterns of lipid-and peptide localizations in plaques and revealed different roles for specific lipids in plaque growth and amyloid fibrillation.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Per Kristian Eide, Aslan Lashkarivand, Are Pripp, Lars Magnus Valnes, Markus Herberg Hovd, Geir Ringstad, Kaj Blennow, Henrik Zetterberg
Summary: In this study, associations between plasma neurodegeneration biomarker concentrations and measures of glymphatic and meningeal lymphatic functions were examined in individuals with neurological disorders. The results suggest that plasma concentrations of neurodegeneration biomarkers are associated with CSF clearance functions.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Patrick M. Wehrli, Junyue Ge, Wojciech Michno, Srinivas Koutarapu, Ambra Dreos, Durga Jha, Henrik Zetterberg, Kaj Blennow, Joerg Hanrieder
Summary: We propose a novel correlative chemical imaging strategy that integrates multimodal matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI), hyperspectral microscopy, and spatial chemometrics. Our approach overcomes challenges associated with data acquisition and alignment by using evolutionary image registration and multiblock orthogonal component analysis. This allows for the identification of covariations of biochemical signatures between and within imaging modalities. We demonstrate the potential of our method in delineating chemical traits of Alzheimer's disease pathology and improving image fusion for correlative MSI and fluorescence microscopy.
Article
Geriatrics & Gerontology
Mariola Zapater-Fajari, Patricia Diaz-Galvan, Nira Cedres, Therese Rydberg Sterner, Lina Ryden, Simona Sacuiu, Margda Waern, Anna Zettergren, Henrik Zetterberg, Kaj Blennow, Silke Kern, Vanesa Hidalgo, Alicia Salvador, Eric Westman, Ingmar Skoog, Daniel Ferreira
Summary: This study found a significant association between depressive symptomatology and SCD-memory and SCD-concentration. Depressive symptomatology was not associated with AD and CVD biomarkers. The phosphorylated tau biomarker and depressive symptomatology predicted SCD-memory, while the A beta 42/40 ratio and depressive symptomatology predicted SCD-concentration.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Cell Biology
Fangfei Qu, Siqi C. Brough, Wojciech Michno, Chioma J. Madubata, Griffin G. Hartmann, Alyssa Puno, Alexandros P. Drainas, Debadrita Bhattacharya, Erwin Tomasich, Myung Chang Lee, Dian Yang, Jun Kim, Maria Peiris-Pages, Kathryn L. Simpson, Caroline Dive, Matthias Preusser, Angus Toland, Christina Kong, Millie Das, Monte M. Winslow, Anca M. Pasca, Julien Sage
Summary: Small-cell lung cancer cells recruit reactive astrocytes to the brain metastases, mimicking the interactions between neurons and astrocytes during brain development. The secreted factor Reelin from SCLC cells plays a crucial role in recruiting astrocytes, which promote the growth of SCLC by secreting neuronal pro-survival factors.
NATURE CELL BIOLOGY
(2023)
Article
Psychiatry
Marco Bucci, Marina Bluma, Irina Savitcheva, Nicholas J. J. Ashton, Konstantinos Chiotis, Anna Matton, Miia Kivipelto, Guglielmo Di Molfetta, Kaj Blennow, Henrik Zetterberg, Agneta Nordberg
Summary: Plasma biomarkers showed promise in discriminating different stages of Alzheimer's disease in research cohorts, but studies in clinical populations are needed. This study investigated the performance of plasma biomarkers in a cohort of memory clinic patients, with GFAP showing the highest discriminatory ability for identifying A & β; + and A & β; - individuals.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Multidisciplinary Sciences
Stefanie Fruhwurth, Line S. Reinert, Carl Oberg, Marcelina Sakr, Marcus Henricsson, Henrik Zetterberg, Soren R. Paludan
Summary: Immunological control of viral infections in the brain, especially through the TREM2 pathway, is crucial for protection against herpes simplex virus type 1 (HSV1) infection and maintenance of brain integrity. The down-regulation of TREM2 expression in HSV1-infected microglia leads to impaired antiviral response and decreased ability to phagocytose infected neurons. This study highlights the importance of TREM2 in the immune defense against viral infections and raises questions about its role in neurological pathologies.
Article
Chemistry, Multidisciplinary
Ambra Dreos, Junyue Ge, Francisco Najera, Behabitu Ergette Tebikachew, Ezequiel Perez-Inestrosa, Kasper Moth-Poulsen, Kaj Blennow, Henrik Zetterberg, Joerg Hanrieder
Summary: This study discovered a new fluorescent probe, NBD1, which can effectively diagnose beta amyloid plaques in Alzheimer's disease and studied its spectral and switching properties. This finding opens up new avenues for using NBD-based fluorescent probes for super-resolution or dual-color imaging and multifunctional microscopy to study the heterogeneity of amyloid plaques.
Article
Clinical Neurology
Tracy Butler, Liangdong Zhou, Ilker Ozsahin, Xiuyuan Hugh Wang, Jacob Garetti, Henrik Zetterberg, Kaj Blennow, Keith Jamison, Mony J. de Leon, Yi Li, Amy Kuceyeski, Sudhin A. Shah
Summary: Using diffusion tensor imaging along perivascular spaces, the study shows reduced glymphatic clearance in subjects with traumatic brain injury compared to healthy controls and its association with injury severity biomarker. The glymphatic system plays a crucial role in clearing waste products and toxins from the brain during sleep. Failure of glymphatic system is thought to contribute to protein deposition in neurodegenerative disorders. The study suggests that understanding glymphatic functioning after traumatic brain injury could lead to novel therapies for better recovery and reduced risk of neurodegeneration.
BRAIN COMMUNICATIONS
(2023)
Review
Clinical Neurology
Elisa Lancini, Lena Haag, Franziska Bartl, Maren Ruehling, Nicholas J. Ashton, Henrik Zetterberg, Emrah Duezel, Dorothea Haemmerer, Matthew J. Betts
Summary: The noradrenergic system undergoes pathological modifications in aging and neurodegenerative diseases, and significant neuronal loss in Alzheimer's disease and Parkinson's disease. A meta-analysis found that individuals with Parkinson's disease had decreased levels of CSF noradrenaline and 3-methoxy-4-hydroxyphenylglycol, as well as noradrenaline transporters availability in the hypothalamus. In Alzheimer's disease dementia, only 3-methoxy-4-hydroxyphenylglycol levels were increased compared to controls. Further studies are needed to understand the association between these biomarkers and clinical symptoms and pathology.
BRAIN COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)
