期刊
JOURNAL OF NEUROCHEMISTRY
卷 113, 期 3, 页码 761-771出版社
WILEY
DOI: 10.1111/j.1471-4159.2010.06643.x
关键词
A beta peptide; Alzheimer's disease; amyloid precursor protein; cytoskeleton network; neuritic trafficking; secretory vesicular pathway
资金
- European Union
- Fundacao para a Ciencia e Tecnologia of the Portuguese Ministry of Science and Technology [REEQ/1023/BIO/2005, POCTI/NSE/33520, PTDC/QUI-BIQ/101317/2008, BD/16071/2004, BPD/44604/2008]
- Center for Cell Biology at University of Aveiro
- Fundação para a Ciência e a Tecnologia [PTDC/QUI-BIQ/101317/2008] Funding Source: FCT
P>A beta is proteolytically produced from the Alzheimer's amyloid precursor protein (APP). Major properties attributed to A beta include neurotoxic effects that contribute to Alzheimer's disease neurodegeneration. However, A beta can also affect APP processing and trafficking that, in neurons, is anterogradelly transported via microtubules in a kinesin-associated manner. Herein we show that A beta can induce accumulation of intracellular sAPP in primary neuronal cultures. Subcellular fractionation studies and immunofluorescence analysis revealed that upon A beta exposure sAPP retention was localized to cytoskeleton associated vesicular structures along the neurite processes, positive for an APP N-terminal antibody and negative for an APP C-terminal antibody. These vesicular structures were also positive for kinesin light chain 1 (KLC). We confirm that A beta alters both actin and microtubule networks. It increases F-actin polymerization and we report for the first time that A beta decreases alpha-tubulin acetylation. The use of cytoskeleton associated drugs partially reversed the A beta-induced effects on sAPP secretion. The data here presented show that A beta causes intracellular sAPP retention by inducing alterations in the cytoskeleton network, thus contributing to impaired APP/sAPP vesicular transport. Moreover, the data strengthens the hypothesis that A beta-induces neurodegeneration and provides a potential mechanism of action, as impaired vesicular and axonal transport have been linked to Alzheimer's disease pathology.
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