Article
Neurosciences
Che-Wei Chang, Yu-Tien Hsiao, Meyer B. Jackson
Summary: This study demonstrates that Synaptophysin (Syp) and synaptogyrin (Syg) play a regulatory role in dense-core vesicle function at various stages, affecting fusion initiation, mode selection between full fusion and kiss-and-run, and influencing the dynamics of both initial and late-stage fusion pores. The transmembrane domain (TMD) of Syp influences small initial fusion pores, while the C-terminal domain influences large late-stage fusion pores, possibly through an interaction with dynamin.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Plant Sciences
Martiniano Maria Ricardi, Niklas Wallmeroth, Cecilia Cermesoni, Dietmar Gerald Mehlhorn, Sandra Richter, Lei Zhang, Josephine Mittendorf, Ingeborg Godehardt, Kenneth Wayne Berendzen, Edda von Roepenack-Lahaye, York-Dieter Stierhof, Volker Lipka, Gerd Juergens, Christopher Grefen
Summary: The final step in secretion is membrane fusion facilitated by SNARE proteins that reside in opposite membranes. The formation of a trans-SNARE complex between one R and three Q coiled-coiled SNARE domains drives the final approach of the membranes providing the mechanical energy for fusion. Biological control of this mechanism is exerted by additional domains within some SNAREs.
Article
Biology
Sebastien Houy, Joana S. Martins, Noa Lipstein, Jakob Balslev Sorensen
Summary: This study investigated the relationship between phorbolesters and Munc13-1 and ubMunc13-2, and found that phorbolesters only stimulated secretion when ubMunc13-2 was dominant. The results showed that DAG/phorbolesters, ubMunc13-2, and Syt7 are important factors for stimulating dense-core vesicle priming.
Article
Chemistry, Multidisciplinary
Mette Galsgaard Malle, Philipp M. G. Loeffler, Soren S-R Bohr, Magnus Berg Sletfjerding, Nikolaj Alexander Risgaard, Simon Bo Jensen, Min Zhang, Per Hedegard, Stefan Vogel, Nikos S. Hatzakis
Summary: In this study, a single-particle combinatorial multiplexed liposome fusion mediated by DNA is presented, enabling multistep and non-deterministic fusion of nanocontainers. Efficient and leakage-free stochastic fusion sequences were observed for arrays of target liposomes functionalized with individual DNA strands and labeled with distinct chromophores. The method allows high-throughput combinatorial multiplex screens using minimal material.
Article
Neurosciences
Maddalena Comini, Juan Sierra-Marquez, Gustavo Guzman, Arne Franzen, Antje Willuweit, Istvan Katona, Patricia Hidalgo, Christoph Fahlke, Raul E. Guzman
Summary: This study investigates the role of two Clcn3 splice variants, ClC-3b and ClC-3c, in granular exocytosis and catecholamine accumulation in adrenal chromaffin cells. The findings show that ClC-3c regulates catecholamine accumulation and contributes to the formation of readily releasable vesicles, while ClC-3b has low efficiency in vesicle priming.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Yueyue Zhang, Jiangbo Liu, Xiuhai Mao, Hongxuan Fan, Fan Li, Shaopeng Wang, Jiang Li, Min Li, Xiaolei Zuo
Summary: This study successfully reconstructed vesicle assemblies that mimic vesicle clusters in living cells using synthetic vesicles and DNA nanorulers, and monitored them using a carbon fiber nanoelectrode. The results showed that the exocytosis of vesicle clusters is critically dependent on the size of clusters.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Keke Hu, Kim Long Le Vo, Fan Wang, Xin Zhang, Chaoyi Gu, Ning Fang, Nhu T. N. Phan, Andrew G. Ewing
Summary: In multicellular organisms, cells communicate through chemical signals, and recent research has discovered the important role of exosomes, which carry cell-dependent molecules, in cellular communication. This study uses amperometry with microelectrodes to monitor the real-time release of single exosomes from living cells and differentiate them from other extracellular vesicles. The results reveal that exosomes released by neuroendocrine cells contain catecholamine transmitters, challenging the traditional view of chemical communication and providing new insights into the molecular biology of exosomes in the neuroendocrine and central nervous systems.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Neurosciences
Amanda Jimenez-Pompa, Sara Sanz-Lazaro, Arik J. Hone, Lola Rueda-Ruzafa, Jose Medina-Polo, Carmen Gonzalez-Enguita, Jesus Blazquez, Cristobal de los Rios, J. Michael McIntosh, Almudena Albillos
Summary: The study found that varenicline increased the exocytosis of secretory vesicles in human and rat chromaffin cells, especially in the presence of nicotine. These results should be taken into consideration when using varenicline in nicotine addiction therapies.
Review
Plant Sciences
Changxin Luo, Yumei Shi, Yun Xiang
Summary: SNARE proteins play essential roles in maintaining cellular homeostasis and regulating root growth and development in Arabidopsis. Recent studies have shown that SNAREs are involved in various fundamental processes and signaling networks related to vesicle trafficking.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Shiyi Wei, Fei Wu, Jing Liu, Wenliang Ji, Xiulan He, Ran Liu, Ping Yu, Lanqun Mao
Summary: This study provides the first direct evidence of neurotoxicity induced by nanoplastics, using single-vesicle electrochemistry. The researchers observed cellular uptake of polystyrene nanoplastics, leading to cell viability loss and reductions in vesicular catecholamine content, exocytotic activity, neurotransmitter release, and fusion pore opening-closing speed. Mechanistic investigations suggest that nanoplastics disrupt F-actin assemblies. This research offers important insights into how nanoplastics affect neurotransmitter secretion and neural functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Rosalia Gonzalez Brito, Pablo Montenegro, Alicia Mendez, Valentina Carabelli, Giulia Tomagra, Ramtin E. Shabgahi, Alberto Pasquarelli, Ricardo Borges
Summary: Platelets are a good material to study exocytosis and have been used to investigate neurological and psychiatric diseases. The release of serotonin by platelets can be observed directly using carbon fiber electrodes. However, this method is tedious due to the limited number of secretory granules released by each platelet.
Review
Biotechnology & Applied Microbiology
Liang Zhang, Xinlin Liang, Tomas Takac, George Komis, Xiaojuan Li, Yuan Zhang, Miroslav Ovecka, Yanmei Chen, Jozef Samaj
Summary: In plants, membrane compartmentalization relies on vesicle trafficking for organelle communication. Membrane proteins involved in vesicle trafficking are dynamic and responsive to environmental changes and cellular signals. Therefore, understanding the localization and dynamics of these proteins is crucial for dissecting vesicular trafficking pathways.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Maria C. Maldifassi, Fanny Momboisse, Maria J. Guerra, Alex H. Vielma, Jaime Maripillan, Ximena Baez-Matus, Carolina Flores-Munoz, Barbara Cadiz, Oliver Schmachtenberg, Agustin D. Martinez, Ana M. Cardenas
Summary: This study proposes a novel mechanism for Panx1 channel activation through functional interactions with the highly calcium-permeable alpha 7 nicotinic acetylcholine receptor (nAChR), and demonstrates the physiological relevance of Panx1 channels and P2X7 receptors in neurosecretion.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Cell Biology
Guoyi Yang, Liuju Li, Yanmei Liu, Kuo Liang, Lisi Wei, Liangyi Chen
Summary: Impaired insulin release in type 2 diabetes is closely related to chronically elevated glucose concentrations. This study found that high glucose delays fusion events, reduces full fusion, and decreases the size of fusion pores, all contributing to compromised insulin secretion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Physiology
Luis M. Gutierrez, Jose Villanueva
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2018)
Article
Biology
Gerardo J. Felix-Martinez, Amparo Gil, Javier Segura, Jose Villanueva, Luis M. Gutierrez
COMPUTERS IN BIOLOGY AND MEDICINE
(2018)
Review
Biochemistry & Molecular Biology
Virginia Garcia-Martinez, Yolanda Gimenez-Molina, Jose Villanueva, Frederic D. Darios, Bazbek Davletov, Luis M. Gutierrez
Article
Medical Laboratory Technology
Inmaculada Lopez-Font, Guillermo Iborra-Lazaro, Raquel Sanchez-Valle, Jose-Luis Molinuevo, Inmaculada Cuchillo-Ibanez, Javier Saez-Valero
CLINICA CHIMICA ACTA
(2019)
Article
Neurosciences
Inmaculada Lopez-Font, Aitana Sogorb-Esteve, Miriam Javier-Torrent, Gunnar Brinkmalm, Mireia Herrando-Grabulosa, Belen Garcia-Lareu, Janina Turon-Sans, Ricardo Rojas-Garcia, Alberto Lleo, Carlos A. Saura, Henrik Zetterberg, Kaj Blennow, Assumpcio Bosch, Xavier Navarro, Javier Saez-Valero
NEUROBIOLOGY OF DISEASE
(2019)
Article
Medical Laboratory Technology
Eline A. J. Willemse, Yannick Vermeiren, Maria-Salud Garcia-Ayllon, Claire Bridel, Peter P. De Deyn, Sebastiaan Engelborghs, Wiesje M. van der Flier, Erwin E. W. Jansen, Inmaculada B. Lopez-Font, Vera Mendes, Bruno Manadas, Naomi de Roeck, Javier Saez-Valero, Eduard A. Struys, Eugeen Vanmechelen, Ulf Andreasson, Charlotte E. Teunissen
CLINICA CHIMICA ACTA
(2019)
Article
Neurosciences
Inmaculada Lopez-Font, Claudia P. Boix, Henrik Zetterberg, Kaj Blennow, Javier Saez-Valero
MOLECULAR NEUROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Yolanda Gimenez-Molina, Virginia Garcia-Martinez, Jose Villanueva, Bazbek Davletov, Luis M. Gutierrez
SCIENTIFIC REPORTS
(2019)
Article
Clinical Neurology
Claudia P. Boix, Inmaculada Lopez-Font, Inmaculada Cuchillo-Ibanez, Javier Saez-Valero
ALZHEIMERS RESEARCH & THERAPY
(2020)
Article
Physiology
Johanna Guadalupe Pena del Castillo, Pedro Segura-Chama, Ruth Rincon-Heredia, Diana Millan-Aldaco, Yolanda Gimenez-Molina, Jose Villanueva, Luis Miguel Gutierrez, Arturo Hernandez-Cruz
Summary: The hypersecretory phenotype of adrenal chromaffin cells in early spontaneously hypertensive rats is mainly due to enhanced Ca2+-induced Ca2+-release. These abnormalities can be traced back to the prehypertensive stage, with an increase in catecholamine exocytosis in prehypertensive SHR compared to age-matched WKY rats. The mechanism responsible for the heterogeneous population of prehypertensive SHR chromaffin cells and the bias towards secreting more medium and large granules remains unexplained.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jose Villanueva, Yolanda Gimenez-Molina, Bazbek Davletov, Luis M. Gutierrez
Summary: Membrane fusion plays a crucial role in intracellular transport and secretion. Traditionally, proteins have been considered the key components in fusion machinery, while lipids were considered structural elements. However, recent studies have shown that intracellular signaling lipid sphingosine and its synthetic derivatives influence neurotransmitter release and organelle fusion, potentially providing new therapeutic approaches for neurodegenerative disorders and neuronal cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jose Villanueva, Manuel Criado, Yolanda Gimenez-Molina, Virginia Gonzalez-Velez, Amparo Gil, Luis Miguel Gutierrez
Summary: This study reveals the association of nAChRs with the secretory active zones in bovine chromaffin cells and demonstrates the dynamic behavior of these receptors during the secretory process. The findings suggest that the mobility of α3β4 nAChR clusters may enhance the efficiency of the secretory response in chromaffin cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)