Article
Cell Biology
Su Xiaoqing, Chen Yinghua, Yuan Xingxing
Summary: Ischemic stroke (IS) is a neurological disorder caused by acute focal trauma, with high mortality and disability, posing a heavy burden on family and society. Autophagy, a self-digesting process, plays a pivotal role in IS by maintaining cellular homeostasis. Non-coding RNAs (ncRNAs), including microRNA, long non-coding RNA, and circular RNA, have been extensively studied for their regulatory role in autophagy in human diseases. Recent studies suggest that ncRNAs-mediated autophagy is involved in the pathophysiological processes of IS, such as cell apoptosis, inflammation, oxidative stress, blood-brain barrier damage, and glial activation, indicating the potential benefit of regulating autophagy by ncRNAs in IS treatment.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Asmaa Mohammed, Olfat G. G. Shaker, Mahmoud A. F. Khalil, Mohammed Gomaa, Shaimaa A. A. Fathy, Abeer K. K. Abu-El-Azayem, Amira Samy, Mahmoud I. I. Aboelnor, Mohamed S. S. Gomaa, Othman M. M. Zaki, Randa Erfan
Summary: This study investigated the potential roles of four ncRNAs (TUG1 and miR-21, NBAT1, and miR-335) as diagnostic biomarkers for acute ischemic stroke (AIS) and their involvement in the disease pathogenesis. The results suggest that these biomarkers could be promising molecular diagnostic markers for AIS, as they were able to discriminate between AIS patients and healthy controls.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Clinical Neurology
Abdullah Al Mamun, Conelius Ngwa, Shaohua Qi, Pedram Honarpisheh, Saumil Datar, Romana Sharmeen, Yan Xu, Louise D. McCullough, Fudong Liu
Summary: The study found that neuronal CD200 can inhibit proinflammatory responses and has a protective effect against stroke injury. Knockout of neuronal CD200 resulted in larger infarct volumes and more severe behavioral deficits after middle cerebral artery occlusion. In CD200 knockout mice, increased lymphocytic infiltration and enhanced microglial proinflammatory responses were observed at 3 and 7 days post-stroke, accompanied by increased microglial phagocytosis activity.
Article
Geriatrics & Gerontology
Miaomiao Liu, Xiaolin Liu, Maorong Zhou, Kai Sun
Summary: circRNA, a recently discovered class of endogenous non-coding RNA, acts as a molecular sponge to alter miRNA levels and is closely related to stroke. This summary focuses on the biological characteristics and expression of circRNA, as well as its impact on outcomes after ischemic stroke.
Review
Biochemistry & Molecular Biology
Xiaobin Shang, Kai Oliver Boeker, Shahed Taheri, Thelonius Hawellek, Wolfgang Lehmann, Arndt F. Schilling
Summary: Osteoarthritis is a chronic disease that lacks disease-modifying treatment, with the Wnt/beta-catenin pathway playing a key role in its pathophysiology. Both excessive stimulation and suppression of this pathway can contribute to the development of OA. MicroRNAs have been found to regulate various cellular processes in different diseases, offering potential insights for the development of miRNA-based therapeutics for OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Diana Amantea, Daniele La Russa, Marialaura Frisina, Francesca Giordano, Chiara Di Santo, Maria Luisa Panno, Giuseppe Pignataro, Giacinto Bagetta
Summary: This study found that severe cerebral ischemia caused by transient MCAo in adult male mice increased inflammatory neutrophils and monocytes in the blood, as well as miRNA-329-5p levels in the plasma. These inflammatory responses could be prevented by ischemic PC induced by 15 min MCAo before the severe insult.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cristina Rodriguez, Jesus Agulla, Maria Delgado-Esteban
Summary: The emerging era of neuroprotective strategies in ischemia/reperfusion has led to a reevaluation of existing studies on neuroprotection against oxidative stress. While exogenous administration of neuroprotective compounds has shown efficacy in various studies, further research is needed to successfully apply these treatments in clinical settings.
NEUROCHEMICAL RESEARCH
(2021)
Article
Cell Biology
Fei Yan, Pinxiao Wang, Xiaojian Yang, Fuli Wang
Summary: This study reveals a new mechanism of Dex neuroprotection in ischemic stroke by regulating the long non-coding RNA HOXA11-AS through the miR-337-3p/Ybx1 signaling pathway, which protects neuronal cells from ischemia/reperfusion-induced apoptosis.
Article
Immunology
Yueman Zhang, Yunlu Guo, Ruqi Li, Tingting Huang, Yan Li, Wanqin Xie, Chen Chen, Weijie Chen, Jieqing Wan, Weifeng Yu, Peiying Li
Summary: Using single-cell RNA sequencing, researchers identified two microglia subclusters, CH25H(+) and OASL(+), that play important roles in ischemic stroke. CH25H(+) microglia exhibits increased phagocytosis and neuroprotective properties, while OASL(+) microglia is associated with neuroinflammation.
JOURNAL OF NEUROINFLAMMATION
(2023)
Review
Biochemistry & Molecular Biology
Carmen del Rio, Joan Montaner
Summary: Current therapies for stroke management are limited, as neuroprotective treatments have shown limited success due to the complex mechanisms of brain cell death in humans. However, various animal species demonstrate prolonged survival of neurons in hypoxic conditions, suggesting potential for new therapeutic approaches for stroke treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yamei Li, Bo Peng, Yi Li, Airu Huang, Yinian Peng, Qian Yu, Yufeng Li
Summary: This study found that miR-203a-3p and miR-153-3p confer neuroprotective effects against ischemic stroke by inhibiting the SRC-dependent MAPK signaling pathway in vivo and in vitro. These microRNAs can alleviate cognitive deficits, apoptosis, oxidative stress, and inflammation.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Cell & Tissue Engineering
Arshi Waseem, Abdul Quaiyoom Khan, Mohsin Ali Khan, Rehan Khan, Shahab Uddin, Johannes Boltze, Syed Shadab Raza
Summary: Stroke is a leading cause of death and disability worldwide, but effective treatments are limited. Abnormal expression of various non-coding RNAs (ncRNAs) has been found after stroke, affecting processes such as neurogenesis, angiogenesis, apoptosis, and autophagy. Understanding the roles and mechanisms of ncRNAs holds promise for future stroke treatments, as they can modify the impact and progression of stroke on a molecular level. Exploring the functions and underlying mechanisms of ncRNAs after stroke may reveal new therapeutic targets and improve diagnostics for stroke.
Review
Biochemistry & Molecular Biology
Yue Liu, Liuding Wang, Guang Yang, Xiansu Chi, Xiao Liang, Yunling Zhang
Summary: Stroke is a leading cause of death and disability worldwide, with the majority of cases being ischemic stroke. The review highlights the role of sirtuins (SIRTs), a group of enzymes involved in various biological processes, in ischemic stroke. The review discusses the distribution, subcellular location, enzyme activity, and regulatory roles of SIRTs in ischemic stroke, as well as potential therapeutic methods based on SIRTs. The data collected in this study has potential implications for clinical and fundamental research on SIRTs and the development of effective therapeutic candidates for ischemic stroke treatment.
Review
Clinical Neurology
Zongyan Cai, Shuo Li, Tianci Yu, Jiahui Deng, Xinran Li, Jiaxin Jin
Summary: This paper outlines the current understanding of the ncRNA regulatory network and reviews the recent evidence for the contribution of ncRNAs in the experimental ischemic stroke model.
FRONTIERS IN NEUROLOGY
(2022)
Article
Oncology
Pingxin Zhang, Hang Gao, Ruping Yan, Lu Yu, Chengxing Xia, Delin Yang
Summary: This study investigates the effect of hsa_circ_0070512 on the function and mechanism of prostate cancer (PCa). Hsa_circ_0070512 was found to be increased in PCa tissues and cells, predominantly located in the cytoplasm of PCa cells. Overexpression of hsa_circ_0070512 significantly increased PCa cell proliferation and migration, while silencing it decreased these processes. Mechanistically, hsa_circ_0070512 acts as a sponge for miR-338-3p, and its regulation of the hedgehog signaling pathways through miR-338-3p enhances PCa growth and migration.
Article
Biochemistry & Molecular Biology
Ashutosh Dharap, Courtney Pokrzywa, Shruthi Murali, Balarama Kaimal, Raghu Vemuganti
JOURNAL OF NEUROCHEMISTRY
(2015)
Article
Neurosciences
Sunil Bhattarai, Fabrizio Pontarelli, Emily Prendergast, Ashutosh Dharap
NEUROBIOLOGY OF DISEASE
(2017)
Article
Neurosciences
Sunil Bhattarai, Ahmed Aly, Kristy Garcia, Diandra Ruiz, Fabrizio Pontarelli, Ashutosh Dharap
MOLECULAR NEUROBIOLOGY
(2019)
Article
Neurosciences
Ashutosh Dharap, Courtney Pokrzywa, Raghu Vemuganti
Article
Endocrinology & Metabolism
Ashuthosh Dharap, Kellie Bowen, Robert Place, Long-Cheng Li, Raghu Vemuganti
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2009)
Article
Biochemical Research Methods
Irina V. Novikova, Ashutosh Dharap, Scott P. Hennelly, Karissa Y. Sanbonmatsu
Article
Biochemistry & Molecular Biology
Suresh L. Mehta, Ashutosh Dharap, Raghu Vemuganti
NEUROCHEMISTRY INTERNATIONAL
(2014)
Article
Multidisciplinary Sciences
Ashutosh Dharap, Courtney Pokrzywa, Shruthi Murali, Gopal Pandi, Raghu Vemuganti
Article
Clinical Neurology
Ashutosh Dharap, Venkata Prasuja Nakka, Raghu Vemuganti
Article
Clinical Neurology
Ashutosh Dharap, Venkata Prasuja Nakka, Raghu Vemuganti
Review
Neurosciences
Aparna Akella, Sunil Bhattarai, Ashutosh Dharap
NEUROMOLECULAR MEDICINE
(2019)
Article
Neurosciences
Sunil Bhattarai, Aparna Akella, Atish Gandhi, Ashutosh Dharap
Summary: Recent studies have shown the widespread transcription of mammalian enhancers into noncoding eRNAs, some of which play central roles in regulating gene expression during development and disease. In cerebral ischemia, the functions of eRNAs are largely unknown. This study identified stroke-induced eRNAs in the post-stroke cortex and demonstrated the involvement of eRNA in modulating post-stroke cortical brain damage, as well as sex-dependent patterns in eRNA expression that may underlie physiological differences in response to stroke between males and females.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Diandra Ruiz, Sunil Bhattarai, Ashutosh Dharap
Summary: Enhancer-derived RNAs (eRNAs) are a new class of long noncoding RNA that modulate enhancer-mediated gene transcription and influence phenotypic outcomes. This study found that a subset of 77 eRNAs exhibit altered expression patterns in the female cortex during stroke, with eRNA_06347 playing a similar role in modulating post-stroke pathophysiology in both males and females. These findings suggest a common underlying role for eRNA_06347 in the two sexes, with potential implications for sex-specific responses to stroke.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)