Article
Chemistry, Multidisciplinary
Christoph Bookmeyer, Ulrich Roehling, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. The study introduces a novel post-ionization scheme based on single-photon induced chemical ionization using pulsed RF-Kr lamps, which effectively improves the ionization efficiency for analytes. Through the interplay with chemical dopants, the signal of various lipid molecules from mammalian tissue sections was significantly boosted.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Kimberly C. Fabijanczuk, James W. Hager, Scott A. McLuckey
Summary: Conventional electrospray ionization (ESI) of mixtures can complicate the analysis of individual components due to the overlap of singly and multiply charged analyte species. To address this challenge, a gas-phase approach was employed to separate singly charged ions from a population of ions of mixed charge states. The method allows for improved signal-to-noise ratio and retention of spatially separated ions within a mass spectrometer.
ANALYTICAL CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Chemistry, Analytical
Bin Hu, Zhong-Ping Yao
Summary: This article reviews the technical aspects and important applications of wooden-tip ESI-MS in various fields. Wooden-tip ESI-MS has shown significant achievements in the past 10 years and has the potential for further development and application.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Michael Goetze, Lukasz Polewski, Leila Bechtella, Kevin Pagel
Summary: This article introduces a 3D-printable nano-electrospray ionization (nano-ESI) source for Bruker instruments, which can be assembled by anyone with access to a 3D printer. The source can be fitted to any Bruker mass spectrometer with an ionBooster ESI source and only requires minor, reversible changes to the original Bruker hardware. The general utility of the source was demonstrated by recording high-resolution MS spectra of small molecules, intact proteins, as well as complex biological samples in negative and positive ion mode on two different Bruker instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Xuetong Qu, Tao Wang, Xingyue Liu, Xinrong Jiang, Xiao Liang, Jianmin Wu
Summary: A dual-mechanism-driven strategy for high-coverage detection of serum lipids was proposed, using a novel SALDI-MS target composed of composite nanostructures. The strategy enabled simultaneous detection of multiple lipid types in a fast analysis. Furthermore, the method demonstrated high sensitivity and specificity in diagnosing hepatocellular carcinoma.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Amanda Gisler, Kapil Dev Singh, Jiafa Zeng, Martin Osswald, Mo Awchi, Fabienne Decrue, Felix Schmidt, Noriane A. Sievi, Xing Chen, Jakob Usemann, Urs Frey, Malcolm Kohler, Xue Li, Pablo Sinues
Summary: Exhaled breath analysis provides valuable molecular-level information and has promising potential for precision medicine. However, the lack of validation in multicenter trials hinders the transition of breath tests into routine clinical practice. In this study, an interoperability framework was developed and applied, revealing a technical variability of approximately 20% and identifying a core signature of the human exhaled metabolome. The results showed high inter-subject variability for certain metabolic classes but relative stability in other regions, such as the TCA cycle. This framework and the overview of metabolic coverage will pave the way for future large-scale multicenter trials.
Review
Spectroscopy
Changfeng Hu, Wenqing Luo, Jie Xu, Xianlin Han
Summary: Lipid research is gaining more attention, and mass spectrometry-based lipidomics approaches are powerful tools for analyzing cellular lipidomes. However, artifacts generated from in-source fragmentation can cause problems in lipidomics analysis, leading to misannotation and false identification. This article summarizes the manifestations of ion source-generated artifacts in three representative lipidomics approaches and discusses strategies to avoid/reduce these artifacts.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Jun Fu, Jixun Li, Yuzhen Sun, Shu Liu, Fengrui Song, Zhongying Liu
Summary: This study investigated the therapeutic effects and mechanism of action of the polysaccharide SCP2 from Schisandra chinensis on AD rats. The results showed that SCP2 improved cognitive dysfunction, oxidative damage, and A beta deposition in the hippocampus. Metabolomics analysis revealed that SCP2 reversed the metabolic profile disorder in AD rats, identifying key metabolites involved in fatty acid metabolism. These findings provide new insights into the potential of SCP2 for treating AD and lay a foundation for future structure-activity studies.
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Trevor B. Romsdahl, Shrikaar Kambhampati, Somnath Koley, Umesh P. Yadav, Ana Paula Alonso, Doug K. Allen, Kent D. Chapman
Summary: The combination of C-13-isotopic labeling and mass spectrometry imaging offers a method to analyze metabolic flux, but presents technical challenges. Results from studying embryos of oilseed crops show greater C-13-isotopic labeling in cotyledons, with isotopic enrichment in PC molecular species suggesting different flux patterns related to fatty acid pathways.
Editorial Material
Biochemistry & Molecular Biology
Rafal Franski, Monika Beszterda-Buszczak
Summary: Villalva et al. evaluated the potential of yarrow extract as a treatment for H. pylori infection. They used the agar-well diffusion bioassay to test the extract's antimicrobial activity. The extract was fractionated into two fractions containing polar phenolic compounds and monoterpenes and sesquiterpenes, respectively. The phenolic compounds were identified using HPLC-ESIMS, but there were some disputed product ions.
Article
Chemistry, Analytical
Xiaoping Zhang, Xiang Ren, Yuan Zhong, Konstantin Chingin, Huanwen Chen
Summary: The study detected ketones in exhaled breath using mass spectrometry and developed a fast, sensitive, noninvasive method for ketone analysis. Results showed higher levels of acetone in the breath of smokers compared to non-smokers.
Article
Multidisciplinary Sciences
Tanja Bien, Krischan Koerfer, Jan Schwenzfeier, Klaus Dreisewerd, Jens Soltwisch
Summary: Molecular analysis on the single-cell level is rapidly growing in the life sciences. This study presents a sensitive approach to single-cell mass spectrometry based on high-resolution imaging and optical microscopy. The multimodal datasets allow for statistical analysis and direct connection of MS data with microscopy information, providing insights into intracellular lipid distributions and subcellular molecular profiles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Ibraam E. Mikhail, Masoomeh Tehranirokh, Andrew A. Gooley, Rosanne M. Guijt, Michael C. Breadmore
Summary: This review summarizes various sample clean-up and analyte enrichment strategies that can be directly coupled with ESI-MS and nano-ESI-MS for the analysis of biological fluids, including solid phase extraction, solid phase micro-extraction, slug flow micro-extraction/nano-extraction, and more.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Medicinal
Karolina Mikulska-Ruminska, Indira Shrivastava, James Krieger, She Zhang, Hongchun Li, Hulya Bayir, Sally E. Wenzel, Andrew P. VanDemark, Valerian E. Kagan, Ivet Bahar
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2019)
Article
Critical Care Medicine
Nahmah Kim-Campbell, Catherine Gretchen, Vladimir B. Ritov, Patrick M. Kochanek, Goundappa K. Balasubramani, Elizabeth Kenny, Mahesh Sharma, Melita Viegas, Clifton Callaway, Valerian E. Kagan, Huelya Bayir
PEDIATRIC CRITICAL CARE MEDICINE
(2020)
Review
Biochemistry & Molecular Biology
V. E. Kagan, Y. Y. Tyurina, W. Y. Sun, I. I. Vlasova, H. Dar, V. A. Tyurin, A. A. Amoscato, R. Mallampalli, P. C. A. van der Wel, R. R. He, A. A. Shvedova, D. Gabrilovich, H. Bayir
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Alexandr A. Kapralov, Qin Yang, Haider H. Dar, Yulia Y. Tyurina, Tamil S. Anthonymuthu, Rina Kim, Claudette M. St Croix, Karolina Mikulska-Ruminska, Bing Liu, Indira H. Shrivastava, Vladimir A. Tyurin, Hsiu-Chi Ting, Yijen L. Wu, Yuan Gao, Galina V. Shurin, Margarita A. Artyukhova, Liubov A. Ponomareva, Peter S. Timashev, Rosario M. Domingues, Detcho A. Stoyanovsky, Joel S. Greenberger, Rama K. Mallampalli, Ivet Bahar, Dmitry I. Gabrilovich, Hulya Bayir, Valerian E. Kagan
NATURE CHEMICAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
George S. Hussey, Catalina Pineda Molina, Madeline C. Cramer, Yulia Y. Tyurina, Vladimir A. Tyurin, Yoojin C. Lee, Salma O. El-Mossier, Mark H. Murdock, Peter S. Timashev, Valerian E. Kagan, Stephen F. Badylak
Article
Environmental Sciences
E. R. Kisin, N. Yanamala, D. Rodin, A. Menas, M. Farcas, M. Russo, S. Guppi, T. O. Khaliullin, I. Iavicoli, M. Harper, A. Star, V. E. Kagan, A. A. Shvedova
Review
Neurosciences
Andrew M. Lamade, Tamil S. Anthonymuthu, Zachary E. Hier, Yuan Gao, Valerian E. Kagan, Hulya Bayir
EXPERIMENTAL NEUROLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Thomas Uray, Cameron Dezfulian, Abigail A. Palmer, Kristin M. Miner, Rehana K. Leak, Jason P. Stezoski, Keri Janesko-Feldman, Patrick M. Kochanek, Tomas Drabek
Summary: The study found that there are differences in cytokine responses in serum, heart, and brain between asphyxial cardiac arrest (ACA) and ventricular fibrillation cardiac arrest (VFCA), but the changes in cytokine levels and secondary injuries caused by the two types of cardiac arrest are similar.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Critical Care Medicine
Kate F. Kernan, Rachel P. Berger, Robert S. B. Clark, R. Scott Watson, Derek C. Angus, Ashok Panigrahy, Clifton W. Callaway, Michael J. Bell, Patrick M. Kochanek, Ericka L. Fink, Dennis W. Simon
Summary: This study investigated the association between serum biomarkers of inflammation and neurological outcomes in children following cardiac arrest. Elevated levels of CNTF and IL-17 were found to be independently associated with unfavorable 6-month neurological outcomes, suggesting a potential role in post-cardiac arrest syndrome pathophysiology.
Article
Critical Care Medicine
Jeremy R. Herrmann, Ericka L. Fink, Anthony Fabio, Alicia K. Au, Rachel P. Berger, Keri Janesko-Feldman, Robert S. B. Clark, Patrick M. Kochanek, Travis C. Jackson
Summary: This study investigated the serum levels of FGF21 and GDF-15 in pediatric cardiac arrest patients and found that both levels increased after cardiac arrest. Additionally, therapeutic hypothermia appeared to augment the effects of FGF21.
Article
Biochemistry & Molecular Biology
Travis C. Jackson, Cameron Dezfulian, Vincent A. Vagni, Jason Stezoski, Keri Janesko-Feldman, Patrick M. Kochanek
Summary: This study evaluated the neuroprotective effects of several unexplored PHLPP inhibitors and found that NSC74429 has neuroprotective effects across multiple injury mechanisms. Further research and optimization of drug dosing are necessary.
Editorial Material
Critical Care Medicine
Patrick M. Kochanek, Dennis W. Simon, Amy K. Wagner
Letter
Cardiac & Cardiovascular Systems
Jeremy R. R. Herrmann, Travis C. C. Jackson, Anthony Fabio, Robert S. B. Clark, Rachel P. P. Berger, Keri L. L. Janesko-Feldman, Patrick M. M. Kochanek, Ericka L. L. Fink, POCCA Investigators
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Cell Biology
Kara Snyder, Kiersten Gorse, Patrick M. Kochanek, Travis C. Jackson
Summary: The inhibition of the pro-death gene RBM5 does not affect neuronal survival in primary neurons, but it does regulate intracellular responses to injury in a sex-dependent manner.
CELL DEATH DISCOVERY
(2023)
Review
Biochemistry & Molecular Biology
Hulya Bayir, Tamil S. Anthonymuthu, Yulia Y. Tyurina, Sarju J. Patel, Andrew A. Amoscato, Andrew M. Lamade, Qin Yang, Georgy K. Vladimirov, Caroline C. Philpott, Valerian E. Kagan
CELL CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)