期刊
JOURNAL OF NEUROCHEMISTRY
卷 108, 期 2, 页码 486-497出版社
WILEY
DOI: 10.1111/j.1471-4159.2008.05780.x
关键词
differentiation; odorant receptor; regulation; transcription
资金
- National Institutes of Health [R01-DC006267]
- NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS [R01DC006267] Funding Source: NIH RePORTER
We have investigated two clonal mouse olfactory placode (OP) cell lines as a model system for studying endogenous odorant receptor (OR) regulation. Both lines can be differentiated into bipolar neurons with transcriptional profiles consistent with mature sensory neurons. We show that single cells exhibit monogenic OR expression like sensory neurons in vivo. Monogenic OR expression is established in undifferentiated cells and persists through differentiation, but OR gene choice is not a clonal property of either cell line. Interestingly, OR RNA shifts from predominantly nuclear to cytoplasma during differentiation of both cell lines. Finally, our data indicate that a restricted subset of OR genes and OR clusters are over-represented in cell populations, suggesting either a pre-existing intrinsic bias in OP founder cells or extrinsic influences arising from culture conditions.
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