Article
Neurosciences
Andrea Ketschek, Sabrina M. Holland, Gianluca Gallo
Summary: This study reveals a novel function of SARM1 in regulating axon branching and cytoskeletal dynamics in sensory neurons. Knockout of SARM1 leads to excessive axon branching and filopodia formation, along with increased rates of axonal actin patch formation and microtubule polymerization. These findings highlight the negative regulatory role of SARM1 in axon morphogenesis.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Chen Chen, Chien-Hsi Chu, Ying Chu, Ting-Ya Chang, Sheng-Wen Chen, Shu-Yang Liang, Yun-Chi Tsai, Bi-Chang Chen, Hsiung-Lin Tu, Pei-Lin Cheng
Summary: Soft tissue environments play an important role in neuronal morphogenesis. This study reveals that drebrin, a growth cone T-zone protein, modulates growth cone turning in response to brain-derived neurotrophic factor (BDNF) on soft substrates. Structurally, axonal growth cones on soft hydrogels have an expanded T zone with integrated drebrin, F-actin and microtubules. Biochemically, paxillin interacts with drebrin specifically on soft hydrogels. Functionally, BDNF-induced growth cone turning and force generation on soft substrates require phosphorylation of drebrin and association of paxillin with drebrin.
Article
Cell Biology
Tanushree Kundu, Priyanka Dutta, Dhriti Nagar, Sankar Maiti, Aurnab Ghose
Summary: The study reveals that Fmn2 plays a crucial role in regulating microtubule stability and dynamic association with actin filaments in growth cones of neurons, thereby influencing cell chemotaxis and chemosensory capabilities of growth cones.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Xubin Hou, Motohiro Nozumi, Harukazu Nakamura, Michihiro Igarashi, Sayaka Sugiyama
Summary: In this study, the researchers investigated the role of the ADF-H domain-containing protein coactosin in axon growth, finding that coactosin promotes the assembly of protrusive actin filament arrays at the leading edge of growth cones. The promotion of axonal growth by coactosin was shown to be dependent on the activity of cofilin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Xi Gu, Chunhong Jia, Junhao Wang
Summary: The establishment and maintenance of neuronal polarity are crucial for neural development and function, and abnormal polarity can lead to neurodevelopmental disorders. Recent studies have made significant progress in understanding the molecular mechanisms of neuronal polarity through positive and negative feedback signals and actin waves. These mechanisms drive the transport and aggregation of key molecules, regulate the interactions of actin filaments and microtubules, promote axon specialization and growth, and inhibit the formation of multiple axons. This review focuses on recent findings regarding neuronal polarity in two classical models, primary hippocampal/cortical neurons in vitro and cortical pyramidal neurons in vivo, and discusses the current understanding of neuronal polarity.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ryan K. Hylton, Jessica E. Heebner, Michael A. Grillo, Matthew T. Swulius
Summary: In this study, the authors demonstrate that filopodial actin filaments switch between bundles of fascin-crosslinked actin and cofilin-decorated filaments. The authors also observe that cofilactin is associated with various dynamic events within filopodia. Furthermore, the hyper-twisting of actin due to cofilin binding leads to a rearrangement of filament packing, which excludes fascin from the base of filopodia.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Joseph Atherton, Melissa Stouffer, Fiona Francis, Carolyn A. Moores
Summary: We used cryo-electron tomography to investigate the subcellular architecture of mouse hippocampal neuron growth cones. Our findings revealed the penetration of hexagonal arrays of actin bundles and the modulation of actin bundles by altering individual F-actin helical structures. Microtubules with different end structures and lattice defects were observed in the growth cone. Absence of the cytoskeleton regulator doublecortin had no major effect on growth cone organization but highlighted the importance of microtubule integrity during growth cone migration.
JOURNAL OF CELL SCIENCE
(2022)
Article
Neurosciences
Rita Pinto-Costa, Monica Mendes Sousa
Summary: Cytolinkers play a crucial role in ensuring the integration of different cytoskeleton components in neurons, which is essential for proper function and connectivity of high order circuits. Several cytoskeleton regulatory proteins with actin-microtubule crosslinking activity have been identified as important players in axon outgrowth and guidance.
NEUROSCIENCE LETTERS
(2021)
Review
Neurosciences
Felix Schneider, Thuy-An Duong, Isabell Metz, Jannik Winkelmeier, Christian A. Hubner, Ulrike Endesfelder, Marco B. Rust
Summary: The study identified CAP1 as a novel actin regulator in growth cones that is crucial for neuron connectivity. Functional interdependence of CAP1 and cofilin1 in neuronal actin dynamics and growth cone function was demonstrated through rescue experiments in double KO neurons.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Cell Biology
Yong-Woo Jun, Soojin Lee, Byung-Kwan Ban, Jin-A Lee, Fen-Biao Gao
Summary: Partial knockdown of MYH10 rescues neurodegeneration in both Drosophila and human iPSC-derived cortical neurons expressing mutant CHMP2B, and MYH10 interacts with ESCRT-III to regulate phagophore closure during mitophagy, revealing novel roles of MYH10 in the autophagy pathway and in ESCRT-related FTD pathogenesis.
Article
Biochemistry & Molecular Biology
Asuncion Carmona, Si Chen, Florelle Domart, Daniel Choquet, Richard Ortega
Summary: Neurons form growth cones during neurodevelopment to establish synaptic connections, and the distributions of F-actin and various biologically active elements in the growth cones play important roles. Calcium is crucial in this process, but other elements are also involved. Deficiencies in these elements, such as iron or zinc, may lead to learning and memory deficits in children.
Article
Neurosciences
Min-Jue Xie, Hideshi Yagi, Tokuichi Iguchi, Hiroyuki Yamazaki, Kenji Hanamura, Hideo Matsuzaki, Tomoaki Shirao, Makoto Sato
Summary: Phldb2 plays an important role in dendritic spine morphology and possibly synaptic plasticity in mature animals, by regulating the localization of drebrin A.
NEUROSCIENCE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Thanushiyan Poobalasingam, Francesca Bianco, Fazal Oozeer, Phillip R. Gordon-Weeks
Summary: The drebrin/EB3 pathway plays a crucial role in neuritogenesis, with loss-of-function leading to reduced dynamic microtubules at the cell periphery and delayed initiation of neuritogenesis, while overexpression inducing supernumerary neurites. EB3 is more closely located to dynamic microtubule plus-ends than EB1 in growth cone filopodia, facilitating continuous microtubule elongation and neuritogenesis.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Gary W. Mathern, Edward H. Bertram
Summary: This study found that neuronal loss associated with limbic epilepsy precedes the onset of seizures and is not a consequence of recurrent seizures. However, intermittent seizures do cause structural changes in the brain, the functional consequences of which are unclear.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Biophysics
Hadrien Oliveri, Alain Goriely
Summary: The establishment of a functioning neuronal network involves the migration, growth, and morphogenesis of neurites, which rely on multiple biophysical effects and sensory cues. Mathematical models are useful for testing hypotheses and understanding the underlying mechanisms of neuron development. This review focuses on different mathematical models for neurite growth and morphogenesis, emphasizing mechanics and mechanisms and simple analytical treatment.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Neurosciences
Yuki Kajita, Nobuhiko Kojima, Noriko Koganezawa, Hiroyuki Yamazaki, Kenji Sakimura, Tomoaki Shirao
EUROPEAN JOURNAL OF NEUROSCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Koichi Uegaki, Haruko Kumanogoh, Toshiyuki Mizui, Takatsugu Hirokawa, Yasuyuki Ishikawa, Masami Kojima
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Review
Biochemistry & Molecular Biology
Tomoaki Shirao, Kenji Hanamura, Noriko Koganezawa, Yuta Ishizuka, Hiroyuki Yamazaki, Yuko Sekino
JOURNAL OF NEUROCHEMISTRY
(2017)
Article
Neurosciences
Noriko Koganezawa, Kenji Hanamura, Yuko Sekino, Tomoaki Shirao
MOLECULAR AND CELLULAR NEUROSCIENCE
(2017)
Article
Cell Biology
Toshiyuki Mizui, Koji Ohira, Masami Kojima
NEURAL REGENERATION RESEARCH
(2017)
Article
Multidisciplinary Sciences
Bin Li, Siyuan Ding, Ningguo Feng, Nancie Mooney, Yaw Shin Ooi, Lili Ren, Jonathan Diep, Marcus R. Kelly, Linda L. Yasukawa, John T. Patton, Hiroyuki Yamazaki, Tomoaki Shirao, Peter K. Jackson, Harry B. Greenberg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemistry & Molecular Biology
Hiroyuki Yamazaki, Yoshio Sasagawa, Hideyuki Yamamoto, Haruhiko Bito, Tomoaki Shirao
JOURNAL OF NEUROCHEMISTRY
(2018)
Article
Neurosciences
Kenji Hanamura, Yousuke Kamata, Hiroyuki Yamazaki, Nobuhiko Kojima, Tomoaki Shirao
Article
Neurosciences
Hiroki Yasuda, Nobuhiko Kojima, Kenji Hanamura, Hiroyuki Yamazaki, Kenji Sakimura, Tomoaki Shirao
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Xiao Li Yin, Qiu Fang Jia, Guang Ya Zhang, Jian Ping Zhang, Tomoaki Shirao, Cai Xia Jiang, Xu Yuan Yin, Yan Song Liu, Peng Chen, Xiao Chu Gu, Zheng Kang Qian, Guang Zhong Yin, Hai Sen Xia, Li Hui
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Daisuke Miki, Yuki Kobayashi, Tomoya Okada, Tatuso Miyamoto, Nobuyuki Takei, Yuko Sekino, Noriko Koganezawa, Tomoaki Shirao, Yumiko Saito
NEUROCHEMICAL RESEARCH
(2019)
Article
Pharmacology & Pharmacy
Toshinari Mitsuoka, Kenji Hanamura, Noriko Koganezawa, Ruri Kikura-Hanajiri, Yuko Sekino, Tomoaki Shirao
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2019)
Article
Pharmacology & Pharmacy
Kenji Hanamura, Noriko Koganezawa, Kazumi Kamiyama, Natsume Tanaka, Takero Oka, Mai Yamamura, Yuko Sekino, Tomoaki Shirao
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2019)
Article
Biochemistry & Molecular Biology
Noriko Koganezawa, Kenji Hanamura, Manuela Schwark, Dilja Krueger-Burg, Hiroshi Kawabe
Summary: The study used 3D-STED microscopy to analyze the morphology of synapses and found an increase in excitatory synapses in specific regions in a mouse model of autism spectrum disorder. This analysis method will open up new areas in developmental neuroscience in the future.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Mai Yamamura, Kenji Hanamura, Noriko Koganezawa, Senami Furubayashi, Tomoaki Shirao, Hiroshi Kawabe
Summary: Methotrexate (MTX) is a commonly used drug for the treatment of acute lymphocytic leukemia or non-Hodgkin lymphoma. However, it can cause neurological side effects such as seizures and cognitive dysfunctions in some patients. The mechanisms behind these side effects, especially on developing neurons, are not well understood.
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)