Article
Medicine, General & Internal
Huiying Jin, Haifeng Li, Shu Qiang
Summary: This study reports two cases of Coffin-Lowry Syndrome in identical twin brothers and investigates their causative mutations. The results show that a mutation in the RPS6KA3 gene is the causative factor for familial CLS, and this variant was detected for the first time in the Chinese population.
MEDICINA-LITHUANIA
(2022)
Article
Neurosciences
Mariateresa Di Stazio, Stefania Bigoni, Nicola Iuso, Josef Vuch, Rita Selvatici, Sheila Ulivi, Pio Adamo d'Adamo
Summary: Unreported missense genetic variant of the ribosomal S6 kinase 2 (RSK2) gene identified in two related males with different phenotypes of intellectual disability (ID) and peculiar facial dysmorphisms. Functional studies demonstrated significant impairment in kinase activity for the two variants involving residue 189.
Article
Biochemistry & Molecular Biology
Eric B. Wright, Shinji Fukuda, Mingzong Li, Yu Li, George A. O'Doherty, Deborah A. Lannigan
Summary: The study identified a natural product, SL0101, that binds specifically to RSK1/2 by inducing conformational rearrangement, but not to RSK3/4 due to a single amino acid difference. Kinetic analysis revealed that regions outside of the N-terminal kinase domain also contribute to stable inhibitor binding towards RSK2. Additionally, a modification on SL0101 was discovered to form a highly stable inhibitor complex with RSK2, suggesting potential for identifying RSK2-specific inhibitors.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Review
Pathology
Junpeng Xu, Qingge Jia, Yan Zhang, Yuan Yuan, Tianqi Xu, Kangjie Yu, Jia Chai, Kaijing Wang, Ligang Chen, Tian Xiao, Mingyang Li
Summary: RSK4 is a Ser/Thr protein kinase that regulates cell survival and proliferation in various types of cancer. Its role as a tumor suppressor gene or an oncogene is controversial, and specific inhibitors for RSK4 have not been found.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Biochemistry & Molecular Biology
Jocelyne Lopez, Daniel A. Bonsor, Matthew J. Sale, Anatoly Urisman, Jennifer L. Mehalko, Miranda Cabanski-Dunning, Pau Castel, Dhirendra K. Simanshu, Frank McCormick
Summary: SPRED proteins negatively regulate the RAS-MAPK pathway and their mutations lead to unique disease phenotypes. Through affinity purification mass spectrometry, the specific interaction between SPRED2 and 90-kDa ribosomal S6 kinase 2 (RSK2) was identified. The structure and functional determinants of this interaction were determined.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Cardiac & Cardiovascular Systems
Muna Suleiman, Afnan Al Najjar, Zain Z. Zakaria, Rashid Ahmed, Huseyin C. Yalcin, Hesham M. Korashy, Shahab Uddin, Sadaf Riaz, Nabeel Abdulrahman, Fatima Mraiche
Summary: Targeted therapy, such as TKIs, has been approved for managing cancer, but TKI-induced cardiotoxicity limits their use. Investigating potential cardioprotective techniques to combine with TKIs has become necessary.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Article
Neurosciences
Samira Ztaou, Soo Jung Oh, Sophia Tepler, Sixtine Fleury, Miriam Matamales, Jesus Bertran-Gonzalez, Nao Chuhma, Stephen Rayport
Summary: This study demonstrates that a single dose of amphetamine has delayed regionally heterogeneous effects on cholinergic interneuron activity, which likely involves extra-striatal synaptic input.
Article
Biochemistry & Molecular Biology
Fabian Arechavaleta-Velasco, Pablo Dominguez-Lopez, Ernesto Acosta-Jimenez, Laura Diaz-Cueto
Summary: RSK4 promoter methylation is increased in malignant ovarian tumors, but this does not regulate its expression in ovarian cancer. Reactivation of RSK4 only reduces cell proliferation in the endometrioid histological subtype.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Oncology
Corinna Kosnopfel, Simone Wendlinger, Heike Niessner, Johannes Siewert, Tobias Sinnberg, Angelika Hofmann, Jonas Wohlfarth, David Schrama, Marion Berthold, Claudia Siedel, Birgit Sauer, Aarthi Jayanthan, Georg Lenz, Sandra E. Dunn, Bastian Schilling, Birgit Schittek
Summary: Inhibition of p90 ribosomal S6 kinases (RSK) has demonstrated anti-tumor effects in malignant melanoma with hyperactivated MAPK signaling, regardless of the genetic subgroups. RSK inhibition not only suppresses tumor cell growth and survival, but also promotes tumor cell differentiation and immunogenicity, leading to enhanced T-cell activation and melanoma cell killing.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Physiology
Jaspreet Kalra, Mykhaylo Artamonov, Hua Wang, Aaron Franke, Zaneta Markowska, Li Jin, Zygmunt S. Derewenda, Ramon J. Ayon, Avril Somlyo
Summary: Phosphorylation of smooth muscle myosin regulatory light chain is a critical switch leading to smooth muscle contraction. Previous studies have focused on the role of myosin light chain kinase (MLCK1) in this process, but our research reveals that p90 ribosomal S6 kinase (RSK2) can also function as an MLCK and play a significant role in regulating smooth muscle contractility. Our findings suggest that RSK2 may be a new target for therapeutic interventions aimed at modulating vascular tone and blood pressure.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Anita Alexa, Peter Sok, Fridolin Gross, Krisztian Albert, Evan Kobori, Adam L. Poti, Gergo Gogl, Isabel Bento, Ersheng Kuang, Susan S. Taylor, Fanxiu Zhu, Andrea Ciliberto, Attila Remenyi
Summary: The herpesvirus protein ORF45 can bind to host kinases ERK and RSK, affecting cell signaling by interfering with the host kinases' docking systems. It uses optimized linear binding motifs to hijack the ERK-RSK complex and manipulate ERK-RSK signaling, resulting in enhanced kinase protection and selective up-regulation of RSK substrate phosphorylation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jie Yang, Daojie Guan, Jiaping Wei, Huichen Ge, Xiaoli Cao, Susu Lv, Xiaoyang Zhou, Yang Zheng, Xiangkun Meng, Jianjun Wang, Kun Qian
Summary: Low concentrations of the insecticide chlorantraniliprole have negative effects on the development and reproduction of fall armyworm. It prolongs larvae duration, decreases larvae and pupae weight, lowers pupation and emergence rates, and reduces female adult fecundity. These effects are caused by the disruption of hormone levels and protein expression in fall armyworm.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Virology
Maxim Cherneha, Johannes Korth, Meike Kaulfuss, Mirko Trilling, Marek Widera, Hana Rohn, Sebastian Dolff, Nina Babel, Andre Hoerning, Andreas Kribben, Oliver Witzke
Summary: Reactivations of BK polyoma virus and human cytomegalovirus can cause complications in renal transplant recipients. The phosphorylation of P70S6k in peripheral lymphocytes is associated with BKPyV reactivations and, to a lesser extent, with HCMV infections in these recipients, independent of immunosuppressive drug levels.
Article
Chemistry, Medicinal
Alejandro M. S. Mayer, Mary L. Hall, Joseph Lach, Jonathan Clifford, Kevin Chandrasena, Caitlin Canton, Maria Kontoyianni, Yeun-Mun Choo, Dev Karan, Mark T. Hamann
Summary: This study revealed that Manzamine A (MZA) selectively inhibited the 90 kDa ribosomal protein kinase S6 (RSK1) with 10-fold higher potency compared to RSK2; the inhibitory effect of MZA on cellular RSK1 and RSK2 protein expression was confirmed in human cervical carcinoma cell lines; computational docking experiments supported MZA's differential binding and selectivity towards RSK1 and RSK2.
Article
Endocrinology & Metabolism
Julia Rehnitz, Birgitta Messmer, Ulrike Bender, Xuan Phuoc Nguyen, Ariane Germeyer, Katrin Hinderhofer, Thomas Strowitzki, Edison Capp
Summary: This study found that the activation of the AKT/mTOR signaling pathway is significantly increased in patients with premature ovarian insufficiency, and it may be associated with the FMR1/mTOR/S6K linkage mechanism.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nicolas Foos, Corinne Maurel-Zaffran, Maria Jesus Mate, Renaud Vincentelli, Matthieu Hainaut, Helene Berenger, Jacques Pradel, Andrew J. Saurin, Miguel Ortiz-Lombardia, Yacine Graba
Article
Biochemical Research Methods
Sebastian Klinke, Nicolas Foos, Jimena J. Rinaldi, Gaston Paris, Fernando A. Goldbaum, Pierre Legrand, Beatriz G. Guimaraes, Andrew Thompson
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2015)
Article
Biochemical Research Methods
Ulrich Zander, Michele Cianci, Nicolas Foos, Catarina S. Silva, Luca Mazzei, Chloe Zubieta, Alejandro de Maria, Max H. Nanao
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2016)
Article
Physics, Multidisciplinary
A. Coati, L. M. G. Chavas, P. Fontaine, N. Foos, B. Guimaraes, P. Gourhant, P. Legrand, J. -P. Itie, P. Fertey, W. Shepard, T. Isabet, S. Sirigu, P. -L. Solari, D. Thiaudiere, A. Thompsona
EUROPEAN PHYSICAL JOURNAL PLUS
(2017)
Article
Biochemical Research Methods
Nicolas Foos, Carolin Seuring, Robin Schubert, Anja Burkhardt, Olof Svensson, Alke Meents, Henry N. Chapman, Max H. Nanao
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Maria J. Mate, Renaud Vincentelli, Nicolas Foos, Didier Raoult, Christian Cambillau, Miguel Ortiz-Lombardia
NUCLEIC ACIDS RESEARCH
(2012)
Article
Multidisciplinary Sciences
Stephanie Hutin, Gianluca Santoni, Ulrich Zander, Nicolas Foos, Sylvain Aumonier, Guillaume Gotthard, Antoine Royant, Christoph Mueller-Dieckmann, Gordon Leonard
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Biochemical Research Methods
Nicolas Foos, Michele Cianci, Max H. Nanao
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2019)
Article
Instruments & Instrumentation
Max Nanao, Shibom Basu, Ulrich Zander, Thierry Giraud, John Surr, Matias Guijarro, Mario Lentini, Franck Felisaz, Jeremy Sinoir, Christian Morawe, Amparo Vivo, Antonia Beteva, Marcus Oscarsson, Hugo Caserotto, Fabien Dobias, David Flot, Didier Nurizzo, Jonathan Gigmes, Nicolas Foos, Ralf Siebrecht, Thomas Roth, Pascal Theveneau, Olof Svensson, Gergely Papp, Bernard Lavault, Florent Cipriani, Ray Barrett, Carole Clavel, Gordon Leonard
Summary: ID23-2 is a beamline at ESRF dedicated to macromolecular crystallography. Its recent upgrades include the use of compound refractive lenses and multilayer mirrors for intense focused microbeam and the improvement of sample environment with advanced sample changer/storage system and high-precision multi-axis diffractometer. It also provides automatic data reduction and analysis for advanced protocols.
JOURNAL OF SYNCHROTRON RADIATION
(2022)
Article
Biochemical Research Methods
Nicolas Foos, Mahmoud Rizk, Max H. Nanao
Summary: The use of single isomorphous replacement (SIR) has decreased due to difficulties in sample preparation and identification of isomorphous native and derivative data sets. This study presents a method that can address these issues and simplify the SIR experiment. By soaking a single heavy atom into a microcrystalline slurry and performing automated serial data collection, a set of data collections with varying heavy-atom occupancies is obtained. Differential merging statistics are utilized to segregate the data sets into 'native' and 'derivative' groups, enabling successful determination of phases experimentally by SIR.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Miguel Ortiz-Lombardia, Nicolas Foos, Corinne Maurel-Zaffran, Andrew J. Saurin, Yacine Graba
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)