Article
Biochemistry & Molecular Biology
Tomoo Suzuki, Yidan Dai, Masanori Ono, Junya Kojima, Toru Sasaki, Hiroshi Fujiwara, Naoaki Kuji, Hirotaka Nishi
Summary: Uterine leiomyomas, the most common pelvic tumors in women of reproductive age, can cause various symptoms and reproductive issues. This study found that UCHL1 expression is significantly higher in leiomyomas than in the myometrium. Inhibition of UCHL1 resulted in downregulation of COL1A1 and COL3A1 expression and decreased migration and contractility of leiomyoma cells. Targeting UCHL1 activity may be a potential therapeutic strategy for uterine leiomyoma.
Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Dan-Dan Wu, Yan-Ming Xu, De-Ju Chen, Zhan-Ling Liang, Xu-Li Chen, Machteld N. Hylkema, Marianne G. Rots, Sheng-Qing Li, Andy T. Y. Lau
Summary: Chronic exposure to cadmium can lead to a variety of diseases including cancer. By studying cadmium-transformed human lung epithelial cells, researchers identified differentially expressed proteins in five subcellular fractions and found that UCHL1 plays a crucial role in suppressing EMT. This suggests that UCHL1 could be a potential therapeutic target for chronic cadmium-induced carcinogenesis.
CELL BIOLOGY AND TOXICOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Penglong Wu, Yifan Li, Mingqi Cai, Bo Ye, Bingchuan Geng, Faqian Li, Hua Zhu, Jinbao Liu, Xuejun Wang
Summary: This study found that UCHL1 plays a crucial protective role in cardiomyocytes by promoting autophagy and reducing cardiac dysfunction after myocardial infarction.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Vyacheslav Akimov, Mirjam Fehling-Kaschek, Inigo Barrio-Hernandez, Michele Puglia, Jakob Bunkenborg, Mogens M. Nielsen, Jens Timmer, Joern Dengjel, Blagoy Blagoev
Summary: Receptor tyrosine kinases (RTK) play crucial roles in cell proliferation and differentiation by binding growth factors, with EGFR being a prototypic RTK that can bind multiple ligands. A proteomics-based workflow combined with mathematical modeling revealed that ligand-specific ubiquitination events on EGFR play a significant role in signal attenuation and termination. The absolute magnitude of EGFR ubiquitination, rather than distinctly regulated ubiquitination sites, is a major determinant for signal attenuation and subsequent cellular outcomes.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Medicine, General & Internal
Sawsan Abuhamdah, Tahia H. Saleem, Bakheet E. M. Elsadek, Omyma Ashraf, Ali R. Hamdan, Eslam El Sayed El-Khateeb, Saeda M. Abd Elwahab, Mohammed H. Hassan
Summary: The study aimed to assess the role of serum UCH-L1 and NGB in predicting severity and outcome of traumatic spinal cord injury (TSCI). The results showed that UCH-L1 could be a helpful marker in reflecting the degree of TSCI and predicting its outcome, while NGB needs further assessment.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Meghan C. H. Ozcan, Lisa Cruz, Morgan F. F. Woodman, Fusun Gundogan, Kathryn J. J. Grive
Summary: The unique deubiquitinating enzyme UCHL1 regulates protein turnover only in oocytes, spermatogonia, and neurons. This study investigates the variation in UCHL1 expression across fetal maturation of oocytes to assess the lifelong ovarian reserve. The study found that UCHL1 expression in oocytes increases during ovarian development and reaches a plateau at 27 weeks gestational age, maintaining elevated levels until 36 weeks. This increase in expression is correlated with the increase in oocyte area, particularly during the transition of oocytes into primordial follicles.
REPRODUCTIVE SCIENCES
(2023)
Article
Medicine, General & Internal
Qing-Hua Zhu, Jing-Kun Wu, Gao-Lei Hou
Summary: This study assessed the changes in glial fibrillary acidic protein (GFAP) and ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) levels in patients with glioma before and after surgery. It was found that UCH-L1 and GFAP levels significantly decreased 3 days after surgery, especially in patients with WHO grade III-IV tumors and tumors larger than 5 cm in diameter. The area under the curve for predicting glioma recurrence was 0.785 and 0.775 for preoperative UCH-L1 and GFAP levels, respectively. These findings suggest that UCH-L1 and GFAP could serve as potential indicators for glioma recurrence and prognosis.
WORLD JOURNAL OF CLINICAL CASES
(2023)
Article
Oncology
Juliana Mueller Bark, Lucas Trevisan Franca de Lima, Xi Zhang, Daniel Broszczak, Paul J. Leo, Rosalind L. Jeffree, Benjamin Chua, Bryan W. Day, Chamindie Punyadeera
Summary: This study demonstrates the feasibility of isolating and characterizing small extracellular vesicles (EVs) from pre- and postoperative saliva samples from glioblastoma (GBM) patients for the first time. Preliminary findings encourage further large cohort validation studies on salivary small EVs to evaluate prognosis in GBM.
Article
Biochemical Research Methods
Sunkyu Choi, Atilio Reyes Romero, Fathima Mashood, Neha Goswami, Lotfi Chouchane, Frank Schmidt
Summary: Protein turnover, the balance between protein synthesis and degradation, is essential for cellular protein homeostasis. This study aimed to analyze protein turnover in adipocytes using time-dependent SILAC labeling. The results revealed associations between protein half-lives and various cellular processes, including energy metabolism, RNA splicing, and Golgi complex. These findings contribute to a better understanding of adipocyte proteome dynamics and protein homeostasis maintenance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Pediatrics
Shuying Zeng, Yubo Huang, Tao Zhong, Tao Huang, Xianyan Dong, Huadong Zhu, Fulian Ouyang
Summary: This study found that the expression levels of serum UCH-L1 were significantly elevated in newborns with HIE, correlated with the severity of brain injury, and negatively associated with the development quotient, intelligence index, and NBNA score of newborns.
TRANSLATIONAL PEDIATRICS
(2021)
Article
Biochemical Research Methods
Rifat Shahriar Sajid, Lennart J. J. van Winden, Phedias Diamandis
Summary: Glioblastoma (GBM) is the most common and severe form of brain cancer among adults, and its aggressiveness is due to its complex and heterogeneous biology. Traditional large-scale profiling approaches have contributed to the understanding of inter-tumoral differences in GBM, but it is now clear that intra-tumoral heterogeneity is also important in treatment resistance and recurrence. The growing geospatial model of GBM layers intra-tumoral heterogeneity on different levels and discusses potential discordances between genotypes and phenotypes in GBM.
Article
Reproductive Biology
Morgan F. Woodman, Meghan C. H. Ozcan, Megan A. Gura, Payton De La Cruz, Alexis K. Gadson, Kathryn J. Grive
Summary: UCHL1 is highly expressed in developing oocytes and plays a crucial role in proper ovarian folliculogenesis, estrous cyclicity, and fertility in female mice. The deficiency of UCHL1 results in reduced litter numbers and sizes, altered folliculogenesis, morphologically abnormal oocytes, disrupted estrous cyclicity, and apparent endocrine dysfunction.
BIOLOGY OF REPRODUCTION
(2022)
Article
Chemistry, Analytical
Qingqing Li, Yang Zhang, Zhen Wu, Jingnan Huang, Ningning Yue, Lin Huang, Xumin Zhang
Summary: The carbodiimide-catalyzed amidation can lead to a +155 Da side modification on tyrosine residues, significantly impacting the identification of tyrosine-containing peptides. Consideration and removal of this side modification can greatly increase the identification rate of tyrosine-containing peptides in proteomic studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Sunkyu Choi, Rudolf Engelke, Neha Goswami, Frank Schmidt
Summary: This study used SILAC-based proteomic profiling to investigate the effects of metformin on 3T3-L1 adipocytes. The results showed that metformin not only affects glucose and lipid metabolism, but also has an impact on protein folding, endoplasmic reticulum stress, negative regulation of appetite, and one-carbon folate metabolism in adipocytes.
Article
Clinical Neurology
Ye Yoon Suh, Kwanghoon Lee, Yu-Mi Shim, Ji Hoon Phi, Chul-Kee Park, Seung-Ki Kim, Seung Hong Choi, Hongseok Yun, Sung-Hye Park
Summary: The MYB/MYBL1::QKI fusion activates the oncogene MYB and deletes the tumor suppressor gene QKI. Previously, this fusion was only reported in angiocentric glioma (AG) and diffuse low-grade glioma. This study compares two tumors with the MYB/MYBL1::QKI fusion: a diffuse pediatric-type high-grade glioma (DPedHGG) in an 11-year-old boy and an AG in a 46-year-old woman. Characterization using immunohistochemistry, next-generation sequencing, and methylation profiling reveals their molecular characteristics and supports the presence of MYB/MYBL1 alterations in higher-grade gliomas.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2023)
Editorial Material
Clinical Neurology
M. E. Buckland, C. Sarkar, V. Santosh, M. Al-Hussaini, S. H. Park, T. Tihan, H. K. Ng, T. Komori
Article
Clinical Neurology
Tae-Hwan Park, Kyung Hyun Kim, Seung-Ki Kim, Kyu-Chang Wang, Sung-Hye Park, Ji Yeoun Lee
Summary: Fibrous hamartoma of infancy (FHI) is a rare benign soft tissue lesion that usually occurs in infants and young children within the first 2 years of life. FHI in the central nervous system (CNS) is extremely rare, with only two cases of spinal cord FHI reported so far. We present a unique case of FHI in a 1-month-old girl with a skin dimple in the coccygeal area, where both a skin lesion and an intramedullary mass were diagnosed as FHI. Despite being diagnosed with a neurogenic bladder, the patient maintained normal neurological function and showed good mobility during the 5.5-year follow-up period.
CHILDS NERVOUS SYSTEM
(2023)
Review
Clinical Neurology
Hyunhee Kim, Kwanghoon Lee, Ji Hoon Phi, Sun Ha Paek, Hongseok Yun, Seung Hong Choi, Sung-Hye Park
Summary: This study presents the clinicopathological and molecular genetic characteristics of a neuroepithelial tumor (NET) with EWSR1::PATZ1 fusion, which has recently been discovered in rare central nervous system tumors and soft tissue sarcomas. Two cases of this fusion were identified, with the pediatric case showing a high-grade tumor and longer survival compared to conventional glioblastoma. The TERT promoter mutation may serve as an indicator of high-grade behavior in PATZ1 fusion NET.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2023)
Article
Medicine, Research & Experimental
Hyunhee Kim, Kwanghoon Lee, Yu -Mi Shim, Eric Eunshik Kim, Seung-Ki Kim, Ji Hoon Phi, Chul-Kee Park, Seung Hong Choi, Sung-Hye Park
Summary: Central neurocytoma is a low-grade neuronal tumor that mainly occurs in the lateral ventricle, and the genetic abnormalities that cause this tumor are poorly understood. Through the study of immunomarkers in fetal and adult brains, as well as supratentorial periventricular tumors, the biomarkers, cell of origin, and tumorigenesis of central neurocytoma were characterized. Immunohistochemical analysis showed that central neurocytomas were positive for certain markers, including FGFR3, SSTR2, TTF-1, GLUT-1, and L1CAM.
LABORATORY INVESTIGATION
(2023)
Article
Oncology
Jung Oh Lee, Sung Soo Ahn, Kyu Sung Choi, Junhyeok Lee, Joon Jang, Jung Hyun Park, Inpyeong Hwang, Chul-Kee Park, Sung Hye Park, Jin Wook Chung, Seung Hong Choi
Summary: This study investigated the prognostic value of spatial features from whole-brain MRI using a three-dimensional convolutional neural network for adult-type diffuse gliomas. The results showed that the MRI-based prognostic index model had good predictive performance, and combining clinical, molecular genetic, and imaging data improved the performance of the prediction model.
Article
Oncology
Jee Ye Kahng, Byung-Hee Kang, Soon-Tae Lee, Seung Hong Choi, Tae Min Kim, Chul-Kee Park, Jae-Kyung Won, Sung-Hye Park, Jaeman Son, Joo Ho Lee
Summary: This study investigated the relationship between neural stem cells (NSCs) in the subventricular zone (SVZ) and glioblastoma (GBM), as well as the impact of radiotherapeutic strategies. The results showed that GBM patients without SVZ involvement had longer progression-free survival, while patients with GBM in contact with NSCs showed better prognosis after NSC irradiation. This study is of great significance for understanding the origin and treatment of GBM.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Oncology
Seung Won Choi, Hyun Ae Jung, Hee-Jin Cho, Tae Min Kim, Chul-Kee Park, Do-Hyun Nam, Se-Hoon Lee
Summary: This study evaluated the therapeutic efficacy of the novel anti-EGFR monoclonal antibody GC1118 in recurrent GBM patients with EGFR amplification. Although the study did not meet the primary endpoint, it was found that immune-related signatures were significantly upregulated in tumors with regression upon GC1118 therapy, indicating the potential immune-mediated antitumor efficacy of GC1118.
Correction
Multidisciplinary Sciences
Soon-Chan Kim, Young-Eun Cho, Young-Kyoung Shin, Hyeon Jong Yu, Tamrin Chowdhury, Sojin Kim, Kyung Sik Yi, Chi-Hoon Choi, Sang-Hoon Cha, Chul-Kee Park, Ja-Lok Ku
Article
Multidisciplinary Sciences
Soon-Chan Kim, Young-Eun Cho, Young-Kyoung Shin, Hyeon Jong Yu, Tamrin Chowdhury, Sojin Kim, Kyung Sik Yi, Chi-Hoon Choi, Sang-Hoon Cha, Chul-Kee Park, Ja-Lok Ku
Summary: Glioblastoma (GBM) is a deadly intracranial tumor. Sequencing technologies have helped in personalized therapy for GBM, but in vitro and in vivo models are needed to validate in silico evaluations of genomic features. GBM cell lines with whole exome sequencing (WES) datasets of matched tumor tissues and blood can be used to investigate molecular markers and validate druggable targets. A complete WES dataset of 26 GBM patient-derived cell lines along with their matched tumor tissues and blood is provided to show that cell lines can largely recapitulate genomic profiles of original tumors.
Article
Biochemistry & Molecular Biology
Hyeongsun Jeong, Hyo Eun Moon, Seongmin Yun, Seung Woo Cho, Hye Ran Park, Sung-Hye Park, Kyungjae Myung, Taejoon Kwon, Sun Ha Paek
Summary: In this study, 13 PDX models derived from 11 brain tumor patients were established. It was found that PDX samples were enriched with brain tumor-related genes and had distinct mutation profiles compared to the original tumors, indicating mutational alterations occurring in the xenograft environment.
Article
Engineering, Biomedical
Yun-Sik Dho, Byeong Cheol Lee, Hyeong Cheol Moon, Kyung Min Kim, Ho Kang, Eun Jung Lee, Min-Sung Kim, Jin Wook Kim, Yong Hwy Kim, Sang Joon Park, Chul-Kee Park
Summary: Concomitant with advances in computing technology, augmented reality-based navigation in clinical applications is being researched. Novel object tracking and depth realization technologies were developed for augmented reality-based neuronavigation in brain surgery. The results validate the efficacy of these technologies in overcoming critical obstacles in the development of clinically applicable augmented reality neuronavigation.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2023)
Review
Clinical Neurology
Chul-Kee Park, Jong Hee Chang
Summary: Recent updates in genomic-integrated glioma classification have caused confusion in clinical practice, but the Korean Brain Tumor Society conducted a questionnaire survey to gain consensus on risk group categorization and treatment preferences. They found agreement on dichotomic definition of risk groups and consistent decisions based on WHO grade and risk group for treatment protocols. Temporary consensus and available options will aid management until more evidence is accumulated under the new classification system.
JOURNAL OF KOREAN NEUROSURGICAL SOCIETY
(2023)
Review
Medicine, General & Internal
Jin Woo Park, Kwanghoon Lee, Eric Eunshik Kim, Seong-Ik Kim, Sung-Hye Park
Summary: The methylation classifier is a tool used to accurately classify brain tumors based on their methylation profiles. It helps healthcare professionals predict tumor prognosis and establish personalized treatment plans. However, it has limitations in classifying all types of brain tumors and faces challenges in obtaining reference data and charging patients for its services.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
