Article
Multidisciplinary Sciences
Salem Al-Mosleh, Ajay Gopinathan, Christian D. Santangelo, Kerwyn Casey Huang, Enrique R. Rojas
Summary: The growth rate of bacteria can adapt to environmental fluctuations through the softening of the cell envelope. This adaptation is only sensitive to local information such as enzyme concentrations and cell envelope curvature. It was also found that the softening of the cell envelope does not occur above a certain magnitude of osmotic shock, and there is direct feedback between cell envelope curvature and envelope expansion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Acoustics
Ziga Pandur, Matevz Dular, Rok Kostanjsek, David Stopar
Summary: This study systematically investigated the resistance of bacterial cells to ultrasonic irradiation by altering the material properties of E. coli cells. The results showed that peptidoglycan, a major component of the cell wall, was the most important determinant of bacterial resistance to sonolysis. Cells without peptidoglycan exhibited high sensitivity to sonolysis.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jianhua Yin, Dan Cheng, Yiling Zhu, Yanqun Liang, Zhiliang Yu
Summary: Developing novel antibiotics to treat infections caused by multi-drug-resistant bacteria is an urgent need. Whole-cell biosensors, which have been extensively studied for environmental monitoring, show promise for detecting cell envelope-acting antibiotics. In this study, a whole-cell biosensor was constructed using the sigma(M)-mediated regulatory system of Bacillus subtilis and successfully detected cell envelope-acting antibiotics with high sensitivity and specificity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andres de Sandozequi, Juan Jose Salazar-Cortes, Iran Tapia-Vazquez, Claudia Martinez-Anaya
Summary: Expansins are a group of proteins found in various organisms, including bacteria and plants, with different roles among different kingdoms. In plants, they remodel the cell wall, while their function in bacteria is challenging to determine. However, genetic evidence from bacterial mutants suggests their involvement in interactions with plants, and the presence of expansin genes in free-living bacteria indicates independent roles. This analysis provides insights into the association of bacterial expansins with the bacterial cell exterior and their potential impact on bacteria-plant interactions and other cell-cell or cell-substrate interactions in free-living species, suggesting a re-evaluation of the current understanding of expansin functions.
Article
Biology
David Ranava, Yiying Yang, Luis Orenday-Tapia, Francois Rousset, Catherine Turlan, Violette Morales, Lun Cui, Cyril Moulin, Carine Froment, Gladys Munoz, Jerome Rech, Julien Marcoux, Anne Caumont-Sarcos, Cecile Albenne, David Bikard, Raffaele Ieva
Summary: Integral outer membrane proteins are crucial for maintaining envelope permeability in Proteobacteria, and DolP, a sigma(E)-upregulated outer membrane lipoprotein, is essential for fitness under envelope stress by interacting with the BAM complex and supporting OMP biogenesis. DolP's association with the mid-cell is linked to regulating septal peptidoglycan remodeling, and during envelope stress, this association is lost, potentially affecting cell division regulation.
Article
Chemistry, Physical
Huanxin Zhang, Huabin Wang, Jonathan J. Wilksch, Richard A. Strugnell, Michelle L. Gee, Xi-Qiao Feng
Summary: This study established a nanoindentation method to measure the turgor pressure and envelope elasticity of live bacteria, and found that the Young's modulus of bacterial envelope is sensitive to the external osmotic environment, and turgor pressure is significantly dependent on the external osmotic stress. This method can not only quantify these mechanical properties of bacteria, but also help understand their behaviors in different environments.
Article
Immunology
Victoria Alves Santos Lunardelli, Bianca da Silva Almeida, Juliana de Souza Apostolico, Thais Rezende, Marcio Massao Yamamoto, Samuel Santos Pereira, Maria Fernanda Campagnari Bueno, Lennon Ramos Pereira, Karina Inacio Carvalho, Renata Dezengrini Slhessarenko, Luis Carlos de Souza Ferreira, Silvia Beatriz Boscardin, Daniela Santoro Rosa
Summary: This study evaluated the features of different recombinant forms of Zika virus proteins produced in bacterial or insect cells. The results showed that proteins produced in bacterial cells had better sensitivity and specificity, and were able to induce higher levels of Zika virus neutralizing antibodies. In addition, proteins produced in insect cells also induced specific cellular immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Sophie McKenna, Kristin Krohn Huse, Sean Giblin, Max Pearson, Mohammed Said Majid Al Shibar, Shiranee Sriskandan, Stephen Matthews, James Edward Pease
Summary: Bacteria, such as streptococci, have evolved sophisticated strategies to evade the host immune system, including the use of proteases like SpyCEP and C5a peptidase to neutralize key immune response molecules such as CXCL8 and C5a. Targeting these proteases through vaccination or small-molecule antagonists may provide protection against streptococcal infections.
JOURNAL OF INNATE IMMUNITY
(2022)
Review
Cell Biology
Sabrina Giacometti, Mark R. MacRae, Kristen Dancel-Manning, Gira Bhabha, Damian C. Ekiert
Summary: Lipid movement within and between membranes is crucial for building and maintaining the bacterial cell envelope. Bacteria have evolved protein-mediated transport systems to facilitate the transport of lipids across membranes.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Eric Mandela, Christopher J. Stubenrauch, David Ryoo, Hyea Hwang, Eli J. Cohen, Von L. Torres, Pankaj Deo, Chaille T. Webb, Cheng Huang, Ralf B. Schittenhelm, Morgan Beeby, J. C. Gumbart, Trevor Lithgow, Iain D. Hay
Summary: The cell envelope of Gram-negative bacteria is composed of two membranes and plays a crucial role in spatial constraints and load-bearing forces. This study focuses on the mechanism behind spatial constraints in Escherichia coli, specifically the role of the coiled-coil lipoprotein Lpp. The findings demonstrate that E. coli can accommodate elongated Lpp through various factors and genetic adaptations, highlighting the importance of maintaining spatial constraints in bacterial cell envelopes.
Article
Genetics & Heredity
Rabia Ladjouzi, Matthieu Duban, Anca Lucau-Danila, Djamel Drider
Summary: This study investigated the role of polynucleotide phosphorylase (PNPase) in the biology of Enterococcus faecalis 14. By obtaining a mutant strain deficient in PNPase synthesis, the researchers found that differentially expressed genes, altered cell envelope ultrastructure, enhanced adhesion capacity on eukaryotic cells, and increased proteolytic activity were observed in the delta pnpA mutant compared to the wild-type strain. These findings suggest that PNPase may directly or indirectly control various biological functions in E. faecalis 14.
Review
Microbiology
Lucy Troman, Ian Collinson
Summary: The article examines the transport process of Gram-negative bacteria inner membrane proteins, as well as the delivery and folding into the periplasm or outer membrane. The mechanism involves interactions between Sec and BAM translocons, possibly with other factors, to provide a conduit to the periplasm and outer membrane.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Alexis G. Sommerfield, Andrew J. Darwin
Summary: Proteases play essential roles in various processes, and the focus of this review is on the carboxyl-terminal processing proteases (CTPs) in bacteria. CTPs have emerged as important enzymes involved in regulation, stress response, remodeling, and virulence in bacteria. The review provides an overview of the roles, structures, and regulation of CTPs in different bacterial species.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Cell Biology
Brandt Warecki, William Sullivan
Summary: A conserved feature of higher eukaryotes is that centromeres are embedded in heterochromatin. This tight association is essential for proper cell division and nuclear envelope formation.
Article
Microbiology
Zhu Zhu, Rui Meng, Walker O. Smith, Hai Doan-Nhu, Lam Nguyen-Ngoc, Xinjun Jiang
Summary: The harmful algal blooms formed by cosmopolitan algae Phaeocystis globosa have been occurring frequently in tropical and subtropical coastal regions in the past two decades. Through high-throughput sequencing, researchers found that the intracolonial fluid of P. globosa possesses a unique microbial environment with lower levels of richness and diversity compared to ambient seawater. The dominant bacterial consortia in the intracolonial fluid were Balneola and Labrezia, involved in degradation of aromatic benzenoid compounds and synthesis of antibacterial compounds.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biology
Andreas Diepold, Judith P. Armitage
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2015)
Editorial Material
Microbiology
Judith P. Armitage
JOURNAL OF BACTERIOLOGY
(2016)
Editorial Material
Microbiology
Judith P. Armitage
JOURNAL OF BACTERIOLOGY
(2016)
Editorial Material
Microbiology
Judith P. Armitage
JOURNAL OF BACTERIOLOGY
(2016)
Article
Microbiology
Amy E. Baker, Andreas Diepold, Sherry L. Kuchma, Jessie E. Scott, Dae Gon Ha, Giulia Orazi, Judith P. Armitage, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2016)
Article
Microbiology
Christopher W. Jones, Judith P. Armitage
JOURNAL OF BACTERIOLOGY
(2017)
Editorial Material
Microbiology
Judith P. Armitage, Anke Becker, Peter J. Christie, Piet A. J. de Boer, Victor J. DiRita, Richard L. Gourse, Tina M. Henkin, William Margolin, William W. Metcalf, Conrad W. Mullineaux, George A. O'Toole, John S. Parkinson, Olaf Schneewind, Thomas J. Silhavy, Ann M. Stock, Igor B. Zhulin
JOURNAL OF BACTERIOLOGY
(2017)
Article
Microbiology
Jessica M. Morgan, Miles C. Duncan, Kevin S. Johnson, Andreas Diepold, Hanh Lam, Allison J. Dupzyk, Lexi R. Martin, Weng Ruh Wong, Judith P. Armitage, Roger G. Linington, Victoria Auerbuch
Article
Microbiology
Amy E. Baker, Shanice S. Webster, Andreas Diepold, Sherry L. Kuchma, Eric Bordeleau, Judith P. Armitage, George A. O'Toole
JOURNAL OF BACTERIOLOGY
(2019)
Article
Microbiology
Nelly Dubarry, Clare R. Willis, Graeme Ball, Christian Lesterlin, Judith P. Armitage
Article
Microbiology
Jia Hui Khoo, Helen Miller, Judith P. Armitage
Summary: Crowding in bacterial cytoplasm can affect cellular processes such as protein folding and molecular diffusion. This study investigates the effects of crowding induced by intracytoplasmic membrane vesicles on Rhodobacter sphaeroides using fluorescence resonance energy transfer (FRET) and photoactivated localization microscopy (PALM). The results show that increased crowding leads to more condensed protein conformation and decreased protein diffusion rates. The findings suggest that physiological activities in bacteria can exhibit different kinetics depending on intracellular membrane organization.
Article
Microbiology
Jia Hui Khoo, Helen Miller, Judith P. Armitage
Summary: The study examined the effects of intracytoplasmic membrane (ICM) vesicles on cellular crowding in Rhodobacter sphaeroides and found that increasing the number of ICM vesicles in low light conditions altered the crowded state of the cytoplasm and decreased protein diffusion rates. This suggests that changes in intracellular membrane organization can impact physiological activities in bacterial species.
