Article
Engineering, Environmental
Xiaolong Yang, Yonghong Bi, Xiaofei Ma, Wei Dong, Xun Wang, Shoubing Wang
Summary: The study highlights the physiological and transcriptomic changes of Microcystis aeruginosa supplied with different nitrogen forms (nitrate and ammonium). The results show that ammonium stimulates initial growth better than nitrate, but excess absorbed light energy under high ammonium leads to cellular damage. Adequate nitrate supply promotes efficient photosynthesis and higher biomass yield. The different nitrogen forms also induce different transcriptomic patterns in M. aeruginosa. These findings provide important clues for understanding the effects of nitrogen forms on cyanobacterial outbreaks.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Multidisciplinary Sciences
Wei-Yue Xing, Jing Liu, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Summary: In this study, a new negative regulator called PatU3 was identified in the prokaryote Anabaena, which regulates cell division and heterocyst development. The results showed that PatU3 is a specific substrate of the protease activity of HetF, and HetF controls cell division and heterocyst differentiation by controlling the inhibitory effects of PatU3.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Di Wu, Ting Wang, Jing Wang, Lijuan Jiang, Ying Yin, Hongyan Guo
Summary: The study reveals that 1 μm polystyrene microplastics have a greater impact on freshwater cyanobacteria compared to 100 nm microplastics, promoting algal growth and inhibiting photosynthesis. Additionally, 1 μm microplastics increase intracellular microcystins content and inhibit their release, while 100 nm microplastics only promote microcystin production.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Daichi Morimoto, Naohiro Yoshida, Aya Sasaki, Satoshi Nakagawa, Yoshihiko Sako, Takashi Yoshida
Summary: The bloom-forming toxic cyanobacterium Microcystis aeruginosa interacts with diverse broad- and narrow-host-range viruses. However, the dynamics of the Microcystis population (at the intraspecies level) and viruses with different host ranges remain unknown.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Soon-Jin Hwang, Yun-Ju Lee, Min-Seob Kim, Baik-Ho Kim
Summary: The study found that Unio douglasiae can effectively intake toxic Microcystis cells, but can only assimilate small amounts of nutrients into tissues within three days, despite a non-flowing system.
APPLIED SCIENCES-BASEL
(2021)
Article
Limnology
Jacqueline Lange, Amelie Cara Berges, Eric von Elert
Summary: Mass developments of toxic cyanobacteria have increased due to global warming and eutrophication, impacting the abundance of herbivory species of Daphnia. The negative effects on Daphnia are attributed to cyanobacterial secondary metabolites, including hepatotoxic microcystins and protease inhibitors. Evidence of local adaptation of Daphnia to cyanobacteria is limited, and this study provides evidence of adaptive tolerance to protease inhibitor-producing cyanobacteria.
LIMNOLOGY AND OCEANOGRAPHY
(2023)
Article
Chemistry, Organic
Akira Ebihara, Arihiro Iwasaki, Youhei Miura, Ghulam Jeelani, Tomoyoshi Nozaki, Kiyotake Suenaga
Summary: The new polyhalogenated aryl sulfates, Bromoiesol sulfates A and B, were isolated from a marine cyanobacterium and their structures were elucidated using Small Molecule Accurate Recognition Technology and X-ray diffraction analysis. Total synthesis of Bromoiesol A sulfate and Bromoiesol A was achieved, and their inhibitory effects on Trypanosoma brucei rhodesiense were confirmed.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Cell Biology
Xiaolong Li, Ge Yang, Wenyao Zhang, Biying Qin, Zifan Ye, Huijing Shi, Xinmeng Zhao, Yihang Chen, Bowei Song, Ziqing Mei, Qi Zhao, Feng Wang
Summary: This article provides an overview of the recent advances in the structure and function of USP13, its relationship with diseases, and the discovery and development of inhibitors. These studies contribute to a better understanding of the molecular mechanisms of USP13 and facilitate the development of more potent inhibitors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Yannik K. -H. Schneider, Anton Liaimer, Johan Isaksson, Oda S. B. Wilhelmsen, Jeanette H. H. Andersen, Kine O. Hansen, Espen H. H. Hansen
Summary: The suomilide and banyasides are non-ribosomal peptides produced by cyanobacteria, with highly modified structures and functionalities. Four suomilides, named suomilide B - E, were isolated and their structures determined. These compounds have different glycosyl group functionalization compared to suomilide A. However, no significant anti-proliferative, anti-biofilm, or anti-bacterial activities were found. Genomic sequencing allowed the proposal of a biosynthetic gene cluster for suomilides, indicating the prephenate-derived azabicyclononane core is most likely incorporated by a proline specific non-ribosomal peptide synthetase-unit.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Jia-Yi Lin, I. -Son Ng
Summary: The halo-tolerant Cyanobacterium PCC 10605 was used for the first time to produce high-level C-phycocyanin (C-PC) with optimized culture conditions. The purified C-PC showed significant antioxidant and antibacterial activities, confirming the potential of PCC 10605 for biotechnological applications.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Poonam Bansal, Raman Kumar, Jasbir Singh, Suman Dhanda
Summary: An alkaline serine protease from Pediococcus acidilactici NCDC 252 was purified and characterized, exhibiting high hydrolyzing activity and stability. Its in-silico characterization revealed similarity with other serine proteases, indicating potential industrial applications.
Article
Biochemistry & Molecular Biology
Caihong Zhang, Jizhen Wei, Zaw Lin Naing, Ei Thinzar Soe, Gemei Liang
Summary: The study identified a serpin-e gene from Helicoverpa armigera, which is widely expressed and highly expressed in fourth-instar larvae and larval hemolymph. It can be induced by Cry1Ac protoxin and inhibits midgut proteases to activate Cry1Ac, contributing to the reduction of Cry1Ac insecticide activity in cotton bollworm. This suggests that serpin-e is involved in the toxicity of Cry1Ac to cotton bollworm by blocking serine protease activation of the protoxin.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Engineering, Chemical
Bo Xu, Lihong Miao, Jing Yu, Lipeng Ji, Hao Lu, Jiangke Yang, Suqin Gao, Yun Kong
Summary: The anti-cyanobacterial microorganism Bacillus amyloliquefaciens T1 produces amino acids Lys and Phe which have been purified and shown to have good anti-cyanobacterial effects with inhibition efficiencies of 94.1% and 80.8% respectively against Microcystis aeruginosa. These amino acids secreted by T1 could potentially be used as a biological agent to control harmful cyanobacterial blooms.
DESALINATION AND WATER TREATMENT
(2021)
Article
Biochemistry & Molecular Biology
Kohta Unno, Hiroyuki Nakagawa, Shinya Kodani
Summary: In this study, a new analogous peptide named marinostatin E was successfully produced using Escherichia coli, exhibiting inhibitory activities against chymotrypsin and subtilisin. The structure of marinostatin E was determined through chemical treatments and tandem mass spectrometry experiments.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Immunology
Mayuko Sakai-Yonaha, Chika Miyagi-Shiohira, Kazuho Kuwae, Yoshihito Tamaki, Kai Nishime, Tasuku Yonaha, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Summary: The study found that preserving porcine pancreata in the MHL solution provided better protection for islet function compared with the UW solution, and the effect of the MHL solution was similar to that of the MK solution.
Article
Chemistry, Organic
Simon D. Schnell, Mauro Schilling, Jan Sklyaruk, Anthony Linden, Sandra Luber, Karl Gademann
Summary: The nucleophilic addition of silyl-enol ethers to nitrogen in 3-monosubstituted s-tetrazines mediated by BF3 was reported, showing preference over commonly observed inverse electron demand Diels-Alder reactions. The substrate dependency of this unusual reaction was rationalized through determination of activation barriers and employing the activation strain model using density functional theory.
Article
Chemistry, Medicinal
Inga S. Shchelik, Andrea Tomio, Karl Gademann
Summary: The study successfully investigated two light-responsive antibiotic derivatives, achieving spatiotemporal control over Gram-positive and Gram-negative bacterial strains, thereby enhancing drug activity and reducing side effects.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
David Dailler, Andrea Dorst, Daniel Schafle, Peter Sander, Karl Gademann
Summary: Fidaxomicin is a marketed antibiotic for CDI treatment, with semi-synthetic modifications presenting a promising strategy to improve its pharmacokinetic properties and circumvent resistance development.
COMMUNICATIONS CHEMISTRY
(2021)
Article
Chemistry, Organic
Simon D. Schnell, Jorge A. Gonzalez, Jan Sklyaruk, Anthony Linden, Karl Gademann
Summary: This study achieved the selective preparation of 3-bromo-pyridazines and successfully synthesized functionalized pyridazines with excellent control over the substitution pattern. Utilizing Lewis acid-mediated inverse electron demand Diels-Alder reaction, the research demonstrated an effective synthetic method for downstream functionalization of 3-bromo-pyridazines.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Gleb A. Chesnokov, Karl Gademann
Summary: The marine-derived compound Peyssonnoside A was successfully synthesized for the first time in a concise, efficient, scalable, and highly diastereoselective manner. The aglucone peyssonnosol was achieved in 21% overall yield after 15 steps, with key reactions including Simmons-Smith cyclopropanation and Mukaiyama hydration controlled by the spatial structure of the substrates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Organic
Lukas Hoff, Simon D. Schnell, Andrea Tomio, Anthony Linden, Karl Gademann
Summary: A silver-mediated Pd-catalyzed cross-coupling method for 3-bromo-1,2,4,5-tetrazine with boronic acids was presented in this study. It was found that electronic modification of the dppf ligand played a crucial role in achieving good turnover. Using this fast method, a variety of alkyl-, heteroatom-, and halide-substituted aryl- and heteroaryl-tetrazines were successfully prepared, with yields up to 87%.
Article
Biochemistry & Molecular Biology
Mathieu Szponarski, Karl Gademann
Summary: Stimulating the immune system against tumor cells is a promising strategy in cancer therapy. Small molecule-based approaches are gaining attention as alternatives to direct immune response, but targeting the balance between immune factors remain challenging. This study demonstrates the use of non-biological hapten to recruit antibodies to cancer cells and shows that metabolic glycoengineering and small molecule stimulators can be combined to direct antibody recognition.
Article
Green & Sustainable Science & Technology
Marcel Mathis, Judith F. Blom, Thomas Nemecek, Esther Bravin, Philippe Jeanneret, Otto Daniel, Laura de Baan
Summary: Plant protection products (PPP) are essential for apple production, but their use and associated risks can be reduced through alternative measures. This study compared different apple production systems based on crop protection strategies in Switzerland. The results showed that no single strategy performed better than others in all indicators assessed.
SUSTAINABLE PRODUCTION AND CONSUMPTION
(2022)
Article
Chemistry, Physical
Lukas Hoff, Gleb A. Chesnokov, Anthony Linden, Karl Gademann
Summary: This study presents a thorough mechanistic investigation of the Pd-catalyzed and Ag-mediated cross-coupling reaction between monosubstituted tetrazine and boronic acids. Through kinetic analyses, isolation of intermediates, and computational methods, insights into the mechanism were obtained. The study also demonstrated the experimental confirmation of a highly electron-deficient ligand and the impact of mechanistic understanding on the generality of the reaction scope through a data-driven workflow.
Article
Chemistry, Organic
Lukas V. Hoff, Joana M. Hauser, Karl Gademann
Summary: An efficient method utilizing Pd catalysis for cross-coupling of 5-bromo-1,2,3-triazine is described. Optimization of reaction conditions enables the preparation of a diverse range of (hetero)aryl-1,2,3-triazines in high yields (up to 97%). Assessment of the reaction scope using data science techniques and subsequent diversification of products allows for the efficient synthesis of pyrimidines and pyridines.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Inga S. Shchelik, Karl Gademann
Summary: This study investigates the effect of newly synthesized cephalosporin derivatives with cyclic disulfide modifications on various strains of bacteria and biofilm formation. The incorporation of asparagusic acid was found to improve the activity of the drug against Gram-negative strains and reduce biofilm formation for Staphylococcus aureus and Pseudomonas aeruginosa. This suggests that incorporating cyclic disulfides may be an additional strategy to enhance antibiotic activity and combat bacterial infections.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Lukas V. Hoff, Simon D. Schnell, Elie Benchimol, Fabio Pereira Foutinho, Andrea Tomio, Michel Rickhaus, Karl Gademann
Summary: This study presents a seven-step protocol for the preparation of monosubstituted 3-bromo-1,2,4,5tetrazine. The procedure starts from commercially readily available starting materials and employs efficient transformations and purification methods. The title compound is obtained on a gram scale with 13% overall yield in reliable purity (>97%), allowing its application in various fields such as bioconjugation, protein labeling, bio-orthogonal chemistry, heterocycle syntheses, high energy materials, and drug release.
HELVETICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Tetiana Studer, Daria Morina, Inga S. Shchelik, Karl Gademann
Summary: This study presents the design and engineering of biohybrid microswimmers that release antibiotics through a thiol-mediated self-immolative mechanism. The microalgae Chlamydomonas reinhardtii were modified with a vancomycin conjugate using a disulfide-based linker. The addition of a thiol-based reducing agent triggered the release of the antibiotic, leading to inhibition of bacterial growth. This is the first example of a microalgae-based drug delivery system utilizing a thiol-mediated, reductive release strategy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Erik Jung, Karl Gademann
Summary: This review discusses small-molecule antibiotics approved for clinical use from 2010 to 2022, including four synthetic antibiotics (bedaquiline, pretomanid, delafloxacin, tedizolid), nine natural product derivatives (ceftaroline fosamil, cefiderocol, plazomicin, omadacycline, eravacycline, sarecycline, lefamulin, dalbavancin, oritavancin), and one natural product (fidaxomicin).
Article
Chemistry, Multidisciplinary
Gleb A. Chesnokov, Karl Gademann
Summary: Terpenoids are a class of natural compounds with diverse structures and bioactivities, which has attracted significant interest from the chemical community in the past 150 years. The presence of strained small rings in terpenoids makes their chemical synthesis challenging due to limited methods and stability issues. This feature article discusses recent examples of total syntheses of terpenoids with complex carbon frameworks featuring small rings, with a focus on the new developments in strategic and tactical approaches to constructing such systems.
CHEMICAL COMMUNICATIONS
(2022)