Article
Chemistry, Applied
Ilya Prokopiev, Igor Sleptsov, Evgeny Serebryakov, Vladimir Sharoyko
Summary: Chemical investigation of the extracts from the red thallus of Cetraria laevigata led to the identification of five known quinoid pigments using FT-IR, UV, NMR, MS, and literature comparison. The antioxidant capacity of these compounds was evaluated and compared to quercetin using various assays. Compounds 2, 4, and 5 showed strong antioxidant activity, comparable to quercetin, while exhibiting weak cytotoxicity in human cancer cells.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Applied
Vinitha Rani, B. C. Revanasiddappa, G. S. Mahendra, Shridhar Deshpande, Jayachandran Venkatesan, Pandurang Appana Dalavi, Ashwini Prabhu
Summary: This study found that the hexane extract of Alkanna tinctoria has potential anticancer activity against glioblastoma cells, and molecular docking studies suggest that certain components in the extract can efficiently bind to brain tumor cell receptors.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Ana Laura Gadelha Castro, Jorddy Neves Cruz, Daniele Ferreira Sodre, Juliana Correa-Barbosa, Rufine Azonsivo, Mozaniel Santana de Oliveira, Jose Edson de Sousa Siqueira, Natasha Costa da Rocha Galucio, Marcelo de Oliveira Bahia, Rommel Mario Rodriguez Burbano, Andrey Moacir do Rosario Marinho, Sandro Percario, Maria Fani Dolabela, Valdicley Vieira Vale
Summary: This study evaluated the genotoxicity and mutagenicity of isoeleutherin and eleutherin in Eleutherine plicata Herb., showing that eleutherin is more genotoxic than isoeleutherin. The molecular docking and MD simulations demonstrated the compounds' ability to interact with TOPO II and form stable complexes, providing new insights into their genotoxic and mutagenic potential.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Saeed Soleimani Asl, Ali Asghar Karkhane, Javad Zamani Amirzakaria, Kambiz Akbari Noghabi, Jaleh Ghashghaie, Kamahldin Haghbeen
Summary: The study showed that the production of POX steadily increased in Alkanna frigida callus through specific culture conditions, leading to the purification of a cationic enzyme called POXalf with optimal activity over a wide pH range and high resistance to H2O2. The specific activity of POXalf in reaction with phenol was found to be over 20 times higher than that of HRP, and it showed 100 times faster reaction with guaiacol compared to phenol. The 3D structure of POXalf was constructed via homology modelling, revealing important differences in active site pockets compared to HRP that could explain the observed differences in substrate spectra and specific activities.
Article
Forestry
Luis Fonseca, Hugo Silva, Isabel Abrantes
Summary: In order to eliminate the pinewood nematode and other nematodes from maritime pine wood, the efficacy of vacuum pressure impregnation (VPI) with commercial wood preservative products was demonstrated through in vitro and industrial assays. The results showed that the three commercial wood preservatives achieved 100% nematode mortality in in vitro nematicidal activity assays. In the industrial assays, VPI treatment resulted in nematode mortality ranging from 99.9761 to 100%. Overall, the VPI process was found to be effective in eliminating nematodes from maritime pine wood.
EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS
(2023)
Article
Pharmacology & Pharmacy
Sethuraman Sakthi Priyadarsini, Vijayalakshmi Palanisami, Gopika Madhusoodanan, B. Velavan, A. M. Yusuf, P. R. Kumar
Summary: In this study, a rhinacanthin enriched extract was obtained from the leaves of Rhinacanthus nasutus by maceration. The extract showed potential cytotoxic activity against neuroblastoma cells in vitro. Preliminary chemical analysis revealed the presence of various secondary metabolites. These findings suggest that further investigation into the molecular mechanism of rhinacanthin as a potential cancer therapeutic is warranted.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Angelique Rat, Henry D. Naranjo, Liesbeth Lebbe, Margo Cnockaert, Nikos Krigas, Katerina Grigoriadou, Eleni Maloupa, Anne Willems
Summary: Two novel bacterial strains isolated from wild plants in Greece were identified as a new species of the genus Roseomonas. Whole genome analysis revealed the presence of genes involved in survival in the rhizosphere environment and plant colonization.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Review
Chemistry, Medicinal
Thais Barreto Santos, Leonardo Gomes Cavalieri de Moraes, Paulo Anastacio Furtado Pacheco, Douglas Galdino dos Santos, Rafaella Machado de Assis Cabral Ribeiro, Caroline dos Santos Moreira, David Rodrigues da Rocha
Summary: This review provides an overview of neuroprotective naphthoquinones, such as shikonin, plumbagin, and vitamin K, which have been found to prevent oxidative stress and exhibit neuroprotective effects through various mechanisms. Synthetic naphthoquinones with inhibitory activity on the P2X7 receptor have also shown neuroprotective effects on Neuro-2a cells. Naphthazarin has been identified as an inhibitor of the MAO-B enzyme, and vitamin K and synthetic naphthoquinones derivatives have demonstrated inhibition of alpha-synuclein aggregation. Synthetic derivatives of juglone and naphthazarin have also been shown to protect Neuro-2a cells against neurodegenerative effects. The review also discusses routes for producing synthetic derivatives, highlighting the potential of 1,4-naphthoquinones as a promising class in the treatment of Parkinson's disease.
Article
Plant Sciences
Jan Schlauer, Siegfried R. H. Hartmeyer, Irmgard Hartmeyer, Tuulikki Seppanen-Laakso, Heiko Rischer
Summary: Dihydronaphthoquinones have been identified as constituents of sundews, Venus flytraps, and dewy pines for the first time. The reduced derivatives may occur in two regio-isomeric series depending on the position of a methyl group, and their presence can distinguish different taxa within the same genus. The joint presence of quinones and hydroquinones allows for observations and predictions on the chemical structures and reactions of these natural products.
Article
Chemistry, Applied
Vanessa Winiewski, Maria Helena Verdan, Cristhian Santos Oliveira, Tayna Eloyane Barreto Rodrigues, Marcos Jose Salvador, Maria Elida Alves Stefanello
Summary: Two new naphthoquinones were isolated from the tubers of Sinningia conspicua, along with nine known compounds. Cytotoxic activity tests showed that all samples were inactive.
NATURAL PRODUCT RESEARCH
(2023)
Article
Environmental Sciences
Shahid Adeel, Shumaila Kiran, Mehboob Alam, Tahir Farooq, Nimra Amin, Tahsin Gulzar
Summary: This study focuses on ultrasonic-assisted dyeing of wool using extracts of Alkanna tinctoria, aiming to enhance color strength and fastness rating. The use of herbal-based extracts as bio-mordant instead of chemical mordants contributes to sustainable dyeing process with good to excellent fastness rating.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agricultural Engineering
Fazal-ur-Rehman, Shahid Adeel, Salman Liaqat, Muhammad Hussaan, Rony Mia, Bulbul Ahmed, Haddar Wafa
Summary: This study investigates the microwave-assisted isolation of Alkannin dye from Alkanna tinctoria and the dyeing process of silk with bio-mordants. The results show that microwave treatment and the use of bio-mordants improve the dyeing performance and colorfastness of silk fabric dyed with Alkannin.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Saud Bawazeer, Abdur Rauf
Summary: This study identified several compounds isolated from Diospyros lotus Linn with in vitro antiglycation and lipoxygenase inhibitory activity, with compounds 4 and 5 showing the highest activity. These compounds demonstrate potential for drug development.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Magdalena Wojciak, Marcin Feldo, Piotr Stolarczyk, Bartosz J. Plachno
Summary: Carnivorous plants use specialized traps to attract and capture small animals or protozoa, which are then killed and digested. The plants absorb the nutrients from the captured organisms to support their growth and reproduction. This review focuses on the secondary metabolites found in Nepenthaceae and Droseraceae, which have been studied using advanced identification techniques. The identified compounds, including phenolic acids, flavonoids, anthocyanins, naphthoquinones, and volatile organic compounds, have shown significant biological activity, highlighting the potential pharmaceutical applications of carnivorous plants.
Article
Biochemistry & Molecular Biology
Mohamad Fawzi Mahomoodally, Gokhan Zengin, Kouadio Ibrahime Sinan, Gunes Ak, Nabeelah Bibi Sadeer, Simone Angeloni, Ahmed M. Mustafa, Giovanni Caprioli, Filippo Maggi, Ugur Cakilcioglu, Alevcan Kaplan, Ebru Yuce Babacan, Abdelhakim Bouyahya, Ekrem Darendelioglu
Summary: The study aimed to validate the phytochemical composition and bioactivity of Alkanna trichophylla and Convolvulus galaticus extracts, finding that homogenizer-assisted extraction was the most efficient technique with high compound yield and promising bioactivities. Types of solvents influenced compound recovery and biological activities, laying the groundwork for further research challenges in cytotoxicity evaluation and molecular docking analysis.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Chemistry, Physical
Fadime Aydogan, El Hassane Anouar, Muhittin Aygun, Hasan Yusufoglu, Canan Karaalp, Erdal Bedir
Summary: The study isolated multiple known compounds and new compounds from the roots and aerial parts of Teucrium sandrasicum plant, elucidating their structures through various analysis methods and testing their cytotoxic activities experimentally.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Biochemical Research Methods
Nilgun Yakubogullari, Furkan Ozan Coven, Nusin Cebi, Fethiye Coven, Nejdet Coven, Rukan Genc, Erdal Bedir, Ayse Nalbantsoy
Summary: AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines.
Article
Plant Sciences
Cigdem Karakoyun, Melis Kucuksolak, Eyup Bilgi, Gamze Dogan, Yigit Ege Comlekci, Erdal Bedir
Summary: Endophytic fungi were found to biotransform oleandrin and nerigoside, leading to the isolation of five new metabolites with two exhibiting potent cytotoxic activity. This study provides important clues for investigating the toxicity mechanisms of these compounds.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Seda Duman, Guner Ekiz, Sinem Yilmaz, Hasan Yusufoglu, Petek Ballar Kirmizibayrak, Erdal Bedir
Summary: Biotransformation of 20(27)-octanor-cycloastragenol by endophytic fungi from Astragalus genus resulted in 15 new transformation products, some of which showed significant telomerase activation effects.
BIOORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Erdal Bedir, Cigdem Karakoyun, Gamze Dogan, Gulten Kuru, Melis Kucuksolak, Hasan Yusufoglu
Summary: Microbial biotransformation of a cytotoxic cardenolide using the endophytic fungus Alternaria eureka resulted in the generation of new metabolites with lower cytotoxicity than the substrate. Among these metabolites, compound 8 exhibited the highest activity against lung cancer cell lines, indicating its potential therapeutic value.
Article
Cell Biology
Lokman Varisli, Aadil Javed, Bilge Esin Ozturk, Gencer Kaan Akyuz, Gulevin Takir, Fani-Marlen Roumelioti, Sarantis Gagos, Kutsal Yorukoglu, Kemal Sami Korkmaz
Summary: Prostate cancer is often associated with alterations in the HN1 gene, which plays a critical role in cell proliferation and cycle regulation. Overexpression of HN1 can lead to differential responses in prostate cancer cells, affecting centrosome clustering and potentially contributing to carcinogenesis.
Article
Plant Sciences
Semiha Cetinel Aksoy, Melis Kucuksolak, Atac Uzel, Erdal Bedir
Summary: A new natural product with pyrrolobenzodiazepine (PBD) framework, 7-methoxy-8-hydroxy cycloanthranilylproline (2), was isolated from marine-derived actinobacterium Streptomyces cacaoi 14CM034, along with cycloanthranilylproline (1). The compounds were structurally elucidated using various analytical techniques and showed notable antimicrobial activity. This study demonstrated the presence of PBD derivatives in S. cacaoi for the first time.
RECORDS OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Emre Gezer, Goklem Uner, Melis Kucuksolak, Mustafa Unver Kurt, Gamze Dogan, Petek Ballar Kirmizibayrak, Erdal Bedir
Summary: Polyether ionophores are a group of naturally occurring compounds mainly produced by Streptomyces species with various bioactivities. In this study, four undescribed and nine known polyether compounds were identified from Streptomyces cacaoi. The antimicrobial activity assays showed strong inhibitory effects of these compounds, including an undescribed compound, on the growth of Bacillus subtilis and methicillin-resistant Staphylococcus aureus (MRSA). Additionally, two compounds displayed significant cytotoxicity, with the C15-demethoxy derivative showing higher selectivity towards cancer cells than K41-A. Mechanistic experiments revealed that the uninvestigated compound acts as a multitarget compound, inhibiting autophagic flux, inducing reactive oxygen species formation, abolishing proteasome activity, and stimulating ER stress.
Article
Microbiology
Fakhra Liaqat, Ismail Hakki Akgun, Mahammed Ilyas Khazi, Rengin Eltem
Summary: This study characterized different chitosanases found in various Bacillus strains and discovered that the chitosanase from B. thuringiensis EGE-B-3.5m showed the best characteristics and COS production potential.
JOURNAL OF BASIC MICROBIOLOGY
(2023)
Article
Plant Sciences
Gulten Kuru, Goklem Uner, Erdal Bedir
Summary: There is a high demand for novel approaches to treat age-related diseases, which are a major cause of death worldwide. Dysfunctional telomeres, which accelerate the aging process, are associated with many of these diseases. Telomerase, an enzyme responsible for maintaining healthy telomeres, has shown promise as a target for treatment. Natural products containing telomerase activators have protective and therapeutic effects against age-related diseases, particularly neurodegenerative, cardiovascular, and osteodegenerative disorders. However, the role of telomerase activation is often overlooked in studies.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Aadil Javed, Mahdieh Yarmohammadi, Kemal Sami Korkmaz, Teresa Rubio-Tomas
Summary: Gastric cancer is characterized by uncontrolled growth and metastasis of gastric epithelial cells, which is predominantly in adenocarcinoma form. The regulation of gastric cell growth is influenced by key cell cycle regulators, including Cyclins and Cyclin-dependent kinases (CDKs). Dysregulation of these molecules can lead to disturbances in cell cycle dynamics and the development of gastric cancer. Numerous studies have examined the roles of Cyclins and CDKs in gastric cancer, and this review aims to compile evidence of upstream regulators that activate or inhibit Cyclins and CDKs. It serves as a comprehensive resource for future studies and hypothesis generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Aadil Javed, Guelseren Ozduman, Lokman Varisli, Bilge Esin Ozturk, Kemal Sami Korkmaz
Summary: HN1 is highly expressed in prostate cancer compared to normal prostate cells. It is involved in the regulation of cell cycle progression at the S-phase and mitotic exit. HN1 interacts with Cdh1 to stabilize and degrade Cyclin B1 through increased ubiquitination.
Article
Plant Sciences
Fadime Aydogan, Zulfiqar Ali, Fazila Zulfiqar, Canan Karaalp, Ikhlas A. Khan, Erdal Bedir
Summary: Fifteen new neo-clerodane diterpenoids, including two undescribed glycosides, as well as a known iridoid glycoside and a phenylpropanoid glycoside, were isolated from the whole plant of Teucrium divaricatum subsp. divaricatum. Their structures were determined through spectral data analysis, and their anti-inflammatory and antimicrobial activities were evaluated. The compounds showed no significant antimicrobial activity against various bacterial and fungal strains, and were inactive up to the highest tested concentration of 50 μM on iNOS inhibitory activity.
PHYTOCHEMISTRY LETTERS
(2023)
Article
Immunology
Nilgun Yakubogullari, Ali Cagir, Erdal Bedir, Duygu Sag
Summary: Astragaloside VII (AST VII), a triterpenic saponin, has potential as a vaccine adjuvant due to its ability to promote a balanced Th1/Th2 immune response. However, the underlying mechanisms of its adjuvant activity have not been fully understood. This study investigated the effects of AST VII and its analogs on human whole blood cells and mouse bone marrow-derived dendritic cells (BMDCs). The results demonstrate that AST VII and its derivatives enhance pro-inflammatory responses, support dendritic cell maturation, and activate T cells in vitro.
Proceedings Paper
Cell & Tissue Engineering
Gulsah Sunal, Gunnur Pulat, Ismail Hakki Akgun, Sinan Guven, Umit Hakan Yildiz, Ozan Karaman, Nesrin Horzum
Summary: The corneal endothelium is a single layer of specialized endothelial cells that protect and nourish the inner surface of the cornea. Damage or loss of these cells can lead to corneal edema and vision loss. Tissue engineering approaches, combined with peptide-modified scaffold surfaces, offer promising alternatives for the regeneration of corneal endothelium tissue.
2022 MEDICAL TECHNOLOGIES CONGRESS (TIPTEKNO'22)
(2022)
