期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 13, 期 1, 页码 417-422出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2013.6917
关键词
Quantum Dots; Nucleus Staining; Cytoplamic Staining; PEGylation; Multiplexing
类别
资金
- Korea Research Foundation
- Korean Government (MEST)
- Gyeonggi-do through National RD Program [K204EA000001-09E0100-00110, 2010-0019107]
- Basic Science Research Program [2010-0024880]
- National Research Foundation of Korea [2007-00559, 2010-0024880] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Developing highly selective probes for subcellular regions such as nucleus and cytoplamic organelles is of great interest for cellular imaging and high content screening analysis for biology and medicine. Cytoplasmic delivery of QDs has been well-understood, while nuclear delivery of QDs has been a challenge due to the unique structural characteristics of cell nucleus. In this study, we systematically investigated nucleus penetrating properties of small-sized ligand-exchanged QDs with either positive or negative surface charges in the similar size range of hydrodynamic diameter (7 similar to 10 nm). We found that the positively-charged QDs efficiently stain the nucleus in fixed He La cells as well as label nucleolar compartments in live He La cells. In contrast, the negatively charged QDs with the similar size range stain only the cytoplam in either fixed or live cells. The charge-dependent labeling pattern allowed us to simultaneously perform multiplex imaging of nuclues and cytoplasm. This study offers an insight into efficient nuclear delivery of nanoparticles such as QDs of which surface charge and size are critical for intracelllar localization and delivery.
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