期刊
PHARMACOLOGY
卷 95, 期 3-4, 页码 105-110出版社
KARGER
DOI: 10.1159/000375452
关键词
Manganese superoxide dismutase; AMP-activated protein kinase; Mitochondrial superoxide; Endothelial cells; Prenylchalcone
资金
- Specialized Research Fund for the National Natural Science Foundation of China [81274130]
- National Natural Science Foundation of China Youth Fund [81102532]
- Doctoral Program of Higher Education of China [20113107110006]
- Innovation Program of the Shanghai Municipal Education Commission of China [12ZZ122]
- Shanghai 085 Project of Higher Education Connotation Construction [085ZY1202]
- Development Program of Key Disciplines of Traditional Chinese Medicine of the Shanghai Municipal Education Commission of China [ZYX-CXYJ-005]
Mitochondrial oxidative stress has been suggested as a major etiological factor in cardiovascular diseases. Manganese superoxide dismutase (MnSOD) is an essential antioxidant mitochondrial enzyme. Although polyphenols can induce MnSOD expression, their mechanism of action remains unclear. We examined the effect of bavachalcone, a bioactive compound isolated from Psoralea corylifolia, on MnSOD protein expression and explored whether this effect is mediated through the AMP-activated protein kinase (AMPK) signaling pathway. Our data showed that bavachalcone enhanced the luciferase activity of the MnSOD promoter and increased MnSOD mRNA and protein expressions. Moreover, bavachalcone suppressed the mitochondrial superoxide production in endothelial cells. Conversely, bavachalcone stimulated liver kinase B1 and AMPK alpha phosphorylation. mRNA interference by using short hairpin RNA (shRNA) of AMPK inhibited bavachalcone-induced MnSOD expression. A-769662, an AMPK activator, also stimulated AMPK activity and increased MnSOD expression. Furthermore, AMPK knockdown by shRNA-AMPK reversed the inhibitory effects of bavachalcone on mitochondrial superoxide production in endothelial cells. These findings indicate that bavachalcone can protect the endothelial function by increasing AMPK activity and MnSOD expression and reducing mitochondrial oxidative stress. (C) 2015 S. Karger AG, Basel
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